This is cutadapt 1.10 with Python 3.5.2
Command line parameters: -p Hm2_GTGAAA_L004/cutadapt/Hm2_GTGAAA_L004_R2_.cutadapt.fastq.gz Hm2_GTGAAA_L004/bwa_bam_to_fastq/Hm2_GTGAAA_L004_R1_.unmapped.fastq.gz Hm2_GTGAAA_L004/bwa_bam_to_fastq/Hm2_GTGAAA_L004_R2_.unmapped.fastq.gz -b file:/home/cokelaer/Work/github/sequana/sequana/resources/data/adapters/adapters_PCRFree_fwd.fa -B file:/home/cokelaer/Work/github/sequana/sequana/resources/data/adapters/adapters_PCRFree_revcomp.fa -m 20 -q 30 -O 6 --trim-n -o Hm2_GTGAAA_L004/cutadapt/Hm2_GTGAAA_L004_R1_.cutadapt.fastq.gz
Trimming 98 adapters with at most 10.0% errors in paired-end mode ...
Finished in 1.98 s (791 us/read; 0.08 M reads/minute).

=== Summary ===

Total read pairs processed:              2,500
  Read 1 with adapter:                     273 (10.9%)
  Read 2 with adapter:                     243 (9.7%)
Pairs that were too short:                 311 (12.4%)
Pairs written (passing filters):         2,189 (87.6%)

Total basepairs processed:       505,000 bp
  Read 1:       252,500 bp
  Read 2:       252,500 bp
Quality-trimmed:                  41,352 bp (8.2%)
  Read 1:        17,366 bp
  Read 2:        23,986 bp
Total written (filtered):        430,536 bp (85.3%)
  Read 1:       216,198 bp
  Read 2:       214,338 bp

=== First read: Adapter Universal_Adapter|name:universal ===

Sequence: AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT; Type: variable 5'/3'; Length: 58; Trimmed: 2 times.
2 times, it overlapped the 5' end of a read
0 times, it overlapped the 3' end or was within the read

No. of allowed errors:
0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40-49 bp: 4; 50-58 bp: 5

Overview of removed sequences (5')
length	count	expect	max.err	error counts
6	1	0.6	0	1
7	1	0.2	0	1


Overview of removed sequences (3' or within)
length	count	expect	max.err	error counts

=== First read: Adapter NextFlex_PCR_Free_adapter1|name:1|seq:CGATGT ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 45 times.
2 times, it overlapped the 5' end of a read
43 times, it overlapped the 3' end or was within the read

No. of allowed errors:
0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40-49 bp: 4; 50-59 bp: 5; 60-63 bp: 6

Overview of removed sequences (5')
length	count	expect	max.err	error counts
6	1	0.6	0	1
11	1	0.0	1	0 1


Overview of removed sequences (3' or within)
length	count	expect	max.err	error counts
6	25	0.6	0	25
11	1	0.0	1	1
18	1	0.0	1	1
22	1	0.0	2	1
25	2	0.0	2	2
26	1	0.0	2	1
28	2	0.0	2	2
33	2	0.0	3	2
34	3	0.0	3	0 3
35	1	0.0	3	0 0 1
36	4	0.0	3	0 0 3 1

=== First read: Adapter NextFlex_PCR_Free_adapter2|name:2|seq:TGACCA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter3|name:3|seq:ACAGTG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter4|name:4|seq:GCCAAT ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter5|name:5|seq:CAGATC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter6|name:6|seq:CTTGTA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter7|name:7|seq:ATCACG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter8|name:8|seq:TTAGGC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter9|name:9|seq:ACTTGA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter10|name:10|seq:GATCAG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter11|name:11|seq:TAGCTT ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter12|name:12|seq:GGCTAC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter13|name:13|seq:AGTCAA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter14|name:14|seq:AGTTCC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter15|name:15|seq:ATGTCA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter16|name:16|seq:CCGTCC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter17|name:17|seq:GTAGAG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 3 times.
0 times, it overlapped the 5' end of a read
3 times, it overlapped the 3' end or was within the read

No. of allowed errors:
0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40-49 bp: 4; 50-59 bp: 5; 60-63 bp: 6

Overview of removed sequences (5')
length	count	expect	max.err	error counts


Overview of removed sequences (3' or within)
length	count	expect	max.err	error counts
38	3	0.0	3	0 0 3

=== First read: Adapter NextFlex_PCR_Free_adapter18|name:18|seq:GTCCGC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter19|name:19|seq:GTGAAA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 223 times.
1 times, it overlapped the 5' end of a read
222 times, it overlapped the 3' end or was within the read

No. of allowed errors:
0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40-49 bp: 4; 50-59 bp: 5; 60-63 bp: 6

Overview of removed sequences (5')
length	count	expect	max.err	error counts
63	1	0.0	6	1


Overview of removed sequences (3' or within)
length	count	expect	max.err	error counts
38	1	0.0	3	0 0 1
39	4	0.0	3	3 1
40	3	0.0	4	3
41	2	0.0	4	2
43	1	0.0	4	1
44	3	0.0	4	3
45	5	0.0	4	4 1
46	1	0.0	4	1
47	3	0.0	4	3
48	1	0.0	4	1
50	1	0.0	5	1
51	3	0.0	5	3
52	3	0.0	5	3
53	2	0.0	5	2
54	1	0.0	5	1
55	2	0.0	5	2
56	3	0.0	5	3
57	1	0.0	5	1
58	5	0.0	5	5
59	2	0.0	5	2
61	4	0.0	6	4
62	9	0.0	6	9
63	8	0.0	6	8
64	9	0.0	6	9
65	7	0.0	6	5 2
66	14	0.0	6	11 3
67	20	0.0	6	19 1
68	30	0.0	6	29 1
69	54	0.0	6	53 1
70	12	0.0	6	12
71	3	0.0	6	3
73	1	0.0	6	1
98	1	0.0	6	0 1
100	3	0.0	6	2 1

=== First read: Adapter NextFlex_PCR_Free_adapter20|name:20|seq:GTGGCC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter21|name:21|seq:GTTTCG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter22|name:22|seq:CGTACG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter23|name:23|seq:GAGTGG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter24|name:24|seq:GGTAGC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter25|name:25|seq:ACTGAT ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter26|name:26|seq:ATGAGC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter27|name:27|seq:ATTCCT ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter28|name:28|seq:CAAAAG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter29|name:29|seq:CAACTA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter30|name:30|seq:CACCGG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter31|name:31|seq:CACGAT ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter32|name:32|seq:CACTCA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter33|name:33|seq:CAGGCG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter34|name:34|seq:CATGGC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter35|name:35|seq:CATTTT ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter36|name:36|seq:CCAACA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter37|name:37|seq:CGGAAT ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter38|name:38|seq:CTAGCT ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter39|name:39|seq:CTATAC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter40|name:40|seq:CTCAGA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter41|name:41|seq:GCGCTA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter42|name:42|seq:TAATCG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter43|name:43|seq:TACAGC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter44|name:44|seq:TATAAT ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter45|name:45|seq:TCATTC ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter46|name:46|seq:TCCCGA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter47|name:47|seq:TCGAAG ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== First read: Adapter NextFlex_PCR_Free_adapter48|name:48|seq:TCGGCA ===

Sequence: GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGCCGTCTTCTGCTTG; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter Universal_Adapter|name:universal ===

Sequence: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT; Type: variable 5'/3'; Length: 58; Trimmed: 243 times.
241 times, it overlapped the 5' end of a read
2 times, it overlapped the 3' end or was within the read

No. of allowed errors:
0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40-49 bp: 4; 50-58 bp: 5

Overview of removed sequences (5')
length	count	expect	max.err	error counts
6	2	0.6	0	2
7	1	0.2	0	1
8	1	0.0	0	1
10	1	0.0	1	1
11	1	0.0	1	1
15	1	0.0	1	1
17	1	0.0	1	1
20	3	0.0	2	3
21	1	0.0	2	1
23	2	0.0	2	2
24	3	0.0	2	3
30	4	0.0	3	3 1
31	1	0.0	3	0 1
33	2	0.0	3	2
34	1	0.0	3	1
36	1	0.0	3	1
37	3	0.0	3	3
38	1	0.0	3	1
39	1	0.0	3	1
40	2	0.0	4	2
42	5	0.0	4	5
43	1	0.0	4	1
44	5	0.0	4	5
45	7	0.0	4	7
47	2	0.0	4	2
48	5	0.0	4	5
49	2	0.0	4	2
50	4	0.0	5	4
51	4	0.0	5	4
53	1	0.0	5	1
54	1	0.0	5	1
55	2	0.0	5	2
57	3	0.0	5	0 3
58	166	0.0	5	164 2


Overview of removed sequences (3' or within)
length	count	expect	max.err	error counts
13	1	0.0	1	1
50	1	0.0	5	1

=== Second read: Adapter NextFlex_PCR_Free_adapter1|name:1|seq:ACATCG ===

Sequence: CAAGCAGAAGACGGCATACGAGATACATCGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter2|name:2|seq:TGGTCA ===

Sequence: CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter3|name:3|seq:CACTGT ===

Sequence: CAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter4|name:4|seq:ATTGGC ===

Sequence: CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter5|name:5|seq:GATCTG ===

Sequence: CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter6|name:6|seq:TACAAG ===

Sequence: CAAGCAGAAGACGGCATACGAGATTACAAGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter7|name:7|seq:CGTGAT ===

Sequence: CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter8|name:8|seq:GCCTAA ===

Sequence: CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter9|name:9|seq:TCAAGT ===

Sequence: CAAGCAGAAGACGGCATACGAGATTCAAGTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter10|name:10|seq:CTGATC ===

Sequence: CAAGCAGAAGACGGCATACGAGATCTGATCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter11|name:11|seq:AAGCTA ===

Sequence: CAAGCAGAAGACGGCATACGAGATAAGCTAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter12|name:12|seq:GTAGCC ===

Sequence: CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter13|name:13|seq:TTGACT ===

Sequence: CAAGCAGAAGACGGCATACGAGATTTGACTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter14|name:14|seq:GGAACT ===

Sequence: CAAGCAGAAGACGGCATACGAGATGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter15|name:15|seq:TGACAT ===

Sequence: CAAGCAGAAGACGGCATACGAGATTGACATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter16|name:16|seq:GGACGG ===

Sequence: CAAGCAGAAGACGGCATACGAGATGGACGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter17|name:17|seq:CTCTAC ===

Sequence: CAAGCAGAAGACGGCATACGAGATCTCTACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter18|name:18|seq:GCGGAC ===

Sequence: CAAGCAGAAGACGGCATACGAGATGCGGACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter19|name:19|seq:TTTCAC ===

Sequence: CAAGCAGAAGACGGCATACGAGATTTTCACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter20|name:20|seq:GGCCAC ===

Sequence: CAAGCAGAAGACGGCATACGAGATGGCCACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter21|name:21|seq:CGAAAC ===

Sequence: CAAGCAGAAGACGGCATACGAGATCGAAACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter22|name:22|seq:CGTACG ===

Sequence: CAAGCAGAAGACGGCATACGAGATCGTACGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter23|name:23|seq:CCACTC ===

Sequence: CAAGCAGAAGACGGCATACGAGATCCACTCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter24|name:24|seq:GCTACC ===

Sequence: CAAGCAGAAGACGGCATACGAGATGCTACCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter25|name:25|seq:ATCAGT ===

Sequence: CAAGCAGAAGACGGCATACGAGATATCAGTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter26|name:26|seq:GCTCAT ===

Sequence: CAAGCAGAAGACGGCATACGAGATGCTCATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter27|name:27|seq:AGGAAT ===

Sequence: CAAGCAGAAGACGGCATACGAGATAGGAATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter28|name:28|seq:CTTTTG ===

Sequence: CAAGCAGAAGACGGCATACGAGATCTTTTGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter29|name:29|seq:TAGTTG ===

Sequence: CAAGCAGAAGACGGCATACGAGATTAGTTGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter30|name:30|seq:CCGGTG ===

Sequence: CAAGCAGAAGACGGCATACGAGATCCGGTGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter31|name:31|seq:ATCGTG ===

Sequence: CAAGCAGAAGACGGCATACGAGATATCGTGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter32|name:32|seq:TGAGTG ===

Sequence: CAAGCAGAAGACGGCATACGAGATTGAGTGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter33|name:33|seq:CGCCTG ===

Sequence: CAAGCAGAAGACGGCATACGAGATCGCCTGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter34|name:34|seq:GCCATG ===

Sequence: CAAGCAGAAGACGGCATACGAGATGCCATGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter35|name:35|seq:AAAATG ===

Sequence: CAAGCAGAAGACGGCATACGAGATAAAATGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter36|name:36|seq:TGTTGG ===

Sequence: CAAGCAGAAGACGGCATACGAGATTGTTGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter37|name:37|seq:ATTCCG ===

Sequence: CAAGCAGAAGACGGCATACGAGATATTCCGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter38|name:38|seq:AGCTAG ===

Sequence: CAAGCAGAAGACGGCATACGAGATAGCTAGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter39|name:39|seq:GTATAG ===

Sequence: CAAGCAGAAGACGGCATACGAGATGTATAGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter40|name:40|seq:TCTGAG ===

Sequence: CAAGCAGAAGACGGCATACGAGATTCTGAGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter41|name:41|seq:TAGCGC ===

Sequence: CAAGCAGAAGACGGCATACGAGATTAGCGCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter42|name:42|seq:CGATTA ===

Sequence: CAAGCAGAAGACGGCATACGAGATCGATTAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter43|name:43|seq:GCTGTA ===

Sequence: CAAGCAGAAGACGGCATACGAGATGCTGTAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter44|name:44|seq:ATTATA ===

Sequence: CAAGCAGAAGACGGCATACGAGATATTATAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter45|name:45|seq:GAATGA ===

Sequence: CAAGCAGAAGACGGCATACGAGATGAATGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter46|name:46|seq:TCGGGA ===

Sequence: CAAGCAGAAGACGGCATACGAGATTCGGGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter47|name:47|seq:CTTCGA ===

Sequence: CAAGCAGAAGACGGCATACGAGATCTTCGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

=== Second read: Adapter NextFlex_PCR_Free_adapter48|name:48|seq:TGCCGA ===

Sequence: CAAGCAGAAGACGGCATACGAGATTGCCGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC; Type: variable 5'/3'; Length: 63; Trimmed: 0 times.

