## ChangeLog - FASTA v35

     $Id: changes_v35.html 120 2010-01-31 19:42:09Z wrp $
     $Revision: 210 $

## Summary - Major Changes in FASTA version 35 (August, 2007)

1. Accurate shuffle based statistics for searches of small libraries (or pairwise comparisons).

2. Inclusion of lalign35 (SIM) into FASTA3. Accurate statistics for lalign35 alignments. plalign has been replaced by lalign35 and lav2ps.

3. Two new global alignment programs: ggsearch35 and glsearch35.

### February 7, 2008

Allow annotations in library, as well as query sequences. Currently, annotations are only available within sequences (i.e., they are not read from the feature table), but they should be available in FASTA format, or any of the other ascii text formats (EMBL/Swissprot, Genbank, PIR/GCG). If annotations are present in a library and the annotation characters includes '*', then the -V '*' option MUST be used. However, special characters other than '*' are ignored, so annotations of '@', '%', or '@' should be transparent.

In translated sequence comparisons, annotations are only available for the protein sequence.

### January 25, 2007

Support protein queries and sequence libraries that contain 'O' (pyrrolysine) and 'U' (selenocysteine). ('J' was supported already). Currently, 'O' is mapped automatically to 'K' and 'U' to 'C'.

### Dec. 13, 2007 CVS fa35_03_02m

Add ability to search a subset of a library using a file name and a list of accession/gi numbers. This version introduces a new filetype, 10, which consists of a first line with a target filename, format, and accession number format-type, and optionally the accession number format in the database, followed by a list of accession numbers. For example:

    	  </slib2/blast/swissprot.lseg 0:2 4|
    	  3121763
    	  51701705
    	  7404340
    	  74735515
    	  ...

Tells the program that the target database is swissprot.lseg, which is in FASTA (library type 0) format.

The accession format comes after the ":". Currently, there are four accession formats, two that require ordered accessions (:1, :2), and two that hash the accessions (:3, :4) so they do not need to be ordered. The number and character after the accession format (e.g. "4|") indicate the offset of the beginning of the accession and the character that terminates the accession. Thus, in the typical NCBI Fasta definition line:

     >gi|1170095|sp|P46419|GSTM1_DERPT Glutathione S-transferase (GST class-mu)

The offset is 4 and the termination character is '|'. For databases distributed in FASTA format from the European Bioinformatics Institute, the offset depends on the name of the database, e.g.

     >SW:104K_THEAN Q4U9M9 104 kDa microneme/rhoptry antigen precursor (p104).

and the delimiter is ' ' (space, the default).

Accession formats 1 and 3 expect strings; accession formats 2 and 4 work with integers (e.g. gi numbers).

### December 10, 2007

Provide encoded annotation information with -m 9c alignment summaries. The encoded alignment information makes it much simpler to highlight changes in critical residues.

### August 22, 2007

A new program is available, `lav2svg`, which creates SVG (Scalable Vector Graphics) output. In addition, `ps_lav`, which was introduced May 30, 2007, has been replaced by `lav2ps`. SVG files are more easily edited with Adobe Illustrator than postscript (`lav2ps`) files.

### July 25, 2007 CVS fa35_02_02

Change default gap penalties for OPTIMA5 matrix to -20/-2 from -24/-4.

### July 23, 2007

Add code to support to support sub-sequence ranges for "library" sequences - necessary for fully functional prss (ssearch35) and lalign35. For all programs, it is now possible to specify a subset of both the query and the library, e.g.

    lalign35 -q mchu.aa:1-74 mchu.aa:75-148

Note, however, that the subset range applied to the library will be applied to every sequence in the library - not just the first - and that the same subset range is applied to each sequence. This probably makes sense only if the library contains a single sequence (this is also true for the query sequence file).

### July 3, 2007 CVS fa35_02_01

Merge of previous `fasta34` with development version `fasta35`.

### June 26, 2007

Add amino-acid 'J' for 'I' or 'L'.

Add Mueller and Vingron (2000) J. Comp. Biol. 7:761-776 VT160 matrix, "-s VT160", and OPTIMA_5 (Kann et al. (2000) Proteins 41:498-503).

### June 7, 2007

`ggssearch35(_t)`, `glsearch35(_t)` can now use PSSMs.

### May 30, 2007 CVS fa35_01_04

Addition of `ps_lav` (now lav2ps or lav2svg) -- which can be used to plot the lav output of `lalign35 -m 11`.

    lalign35 -m 11 | lav2ps

replaces `plalign` (from `FASTA2`).

### May 2, 2007

The labels on the alignment scores are much more informative (and more diverse). In the past, alignment scores looked like:

    >>gi|121716|sp|P10649|GSTM1_MOUSE Glutathione S-transfer  (218 aa)
     s-w opt: 1497  Z-score: 1857.5  bits: 350.8 E(): 8.3e-97
    Smith-Waterman score: 1497; 100.0% identity (100.0% similar) in 218 aa overlap (1-218:1-218)
    ^^^^^^^^^^^^^^

where the highlighted text was either: "Smith-Waterman" or "banded Smith-Waterman". In fact, scores were calculated in other ways, including global/local for `fasts` and `fastf`. With the addition of `ggsearch35,` `glsearch35,` and `lalign35,` there are many more ways to calculate alignments: "Smith-Waterman" (ssearch and protein fasta), "banded Smith-Waterman" (DNA fasta), "Waterman-Eggert", "trans. Smith-Waterman", "global/local", "trans. global/local", "global/global (N-W)". The last option is a global global alignment, but with the affine gap penalties used in the Smith-Waterman algorithm.

### April 19, 2007 CVS fa34t27br_lal_3

Two new programs, `ggsearch35(_t)` and `glsearch35(_t)` are now available. `ggsearch35(_t)` calculates an alignment score that is global in the query and global in the library; `glsearch35(_t)` calculates an alignment that is global in the query and local, while local in the library sequence. The latter program is designed for global alignments to domains. Both programs assume that scores are normally distributed. This appears to be an excellent approximation for ggsearch35 scores, but the distribution is somewhat skewed for global/local (glsearch) scores. `ggsearch35(_t)` only compares the query to library sequences that are beween 80% and 125% of the length of the query; glsearch limits comparisons to library sequences that are longer than 80% of the query. Initial results suggest that there is relatively little length dependence of scores over this range (scores go down dramatically outside these ranges).

### March 29, 2007 CVS fa34t27br_lal_1

At last, the `lalign` (SIM) algorithm has been moved from FASTA21 to FASTA35. A `plalign` equivalent is also available using `lalign -m 11 | lav2ps` or `| lav2svg`. The statistical estimates for `lalign35` should be much more accurate than those from the earlier lalign, because lambda and K are estimated from shuffles. In addition, all programs can now generate accurate statistical estimates with shuffles if the library has fewer than 500 sequences. If the library contains more than 500 sequences and the sequences are related, then the -z 11 option should be used. p

FASTA v34 Change Log