Changes in version 1.15.02 (2021-09-14)
- Update type: minor.
- Various error message updates

Changes in version 1.15.01 (2021-09-01)
- Update type: minor.
- Version bump due to Bioconductor release.
- preFilter() now applies the gene expression cutoff to both conditions instead of the overall average.
- analyzePFAM() was updated to reflect recent updates to the tidyverse read_fwf function. It furhtermore now better distinguishes tap seperated and fixed with files.

Changes in version 1.13.07 (2021-05-06)
- Update type: minor.
- importGTF() and importRdata() was updated to handle the rare cases of mixed stranded and unstranded isoforms (unstanded are now discareded).
- addORFfromGTF() was updated to better repport if no or only small number of ORFs were added.
- Various maintainance updates.

Changes in version 1.13.06 (2021-04-09)
- Update type: minor.
- The runtimes repported by isoformSwitchTestDEXSeq() was updated to also consider the number of transcripts analysed.
- analyzeORF() was updated to enable analysis with analyzeNovelIsoformORF() when no overlaps were found.
- switchPlot was fixed so the alphas argument now work.
- Various updates of warning, descriptions and error messages.
- extractSequence() was updated to remove the terminal stop codon if it is included in the annoation.
- extractSequence() was updated to produce evenly sized files when alsoSplitFastaFile=TRUE.
- analysORF no longer allows identification of truncated ORFs.

Changes in version 1.13.05 (2021-01-07)
- Update type: Major.
- analyseORF was updated with the orfMethod "longest.AnnotatedWhenPossible" a hybrid between "longes" and "longestAnnotated". See ?analyseORF for details.
- importGTF, importRdata and analyseORF was updated to also annoate the source of the ORF annoations. analyzeCPAT and analyzeCPC2 was updated to also changes these if removeNoncodinORFs = TRUE.
- To enable better ORF analysis the addORFfromGTF() and analyzeNovelIsoformORF() functions were added to IsoformSwitchAnalyzeR. These should be used instead of analyzeORF(). These function also annotate the source of the ORF annoations. See vignette for description of why these are preferable.
- analyseORF() was updated with an additional method for ORF detection: "longest.AnnotatedWhenPossible".
- the getCDS() function and CDSSet class was removed for the user as addORFfromGTF() + analyzeNovelIsoformORF() provides a better way to analyse ORFs.
- Downstream functions relying on ORF data now checks that all isoforms have been assessed for ORFs. These are extractSequence(), analyzeSwitchConsequences(), switchPlotTranscript() and switchPlot().
- isoformSwitchAnalysisPart1() and isoformSwitchAnalysisPart2() was also updated to support the new ORF annotation scheme.
- The usage of isoformSwitchAnalysisPart1() and isoformSwitchAnalysisPart2() was made less complex by removing many arguments passed to sub-functions thereby relying more on default arguments.
- importRdata() was updated to import the "refrence gene_ids" instead of StringTie gene_ids (for all annotated genes).
- the StringTie annotation rescue in importRdata() was updated to use "refrence gene_ids" instead of "refrence gene_names" thereby fixing problems with closely spaced genes, that have the same gene name, which was merged by StringTie.
- importGTF() now also imports ref_gene_id from StringTie gtf to enable the above mentioned updates to importRdata(). If not pressent it will duplicate gene_name instead.
- extractGeneExpression() was updated to allow easy output of gene annoation.
- isoformToGeneExp() was updated to use rowsum() instead of a tidyverse implementation as it is much faster for large datasets.
- The result of importRdata()'s estimateDifferentialGeneRange option now repports the condition names in accordance with the rest of IsoformSwitchAnalyzeR.
- Removed mentions of StringTie2 as it has been merged into StringTie.
- Documentation and vignette was updated accordingly.

Changes in version 1.13.04 (2020-12-10)
- Update type: minor.
- Vignette update.

Changes in version 1.13.03 (2020-12-08)
- Update type: minor.
- Vignette update.

Changes in version 1.13.02 (2020-12-07)
- Update type: minor.
- Description update.
- Update of vignette with regards to running on analysis Gallaxy.

Changes in version 1.13.01 (2020-10-29)
- Update type: minor.
- Version bump due to Bioconductor release.
- Fixed an error in importRdata() that could cause trouble when fixing StringTie annotation. Thanks to @yaccos for identifying the problem.
- Fixed an edge-case senario where the estimation of DTU in importRdata() caused an error.
- analyseSignalP() was updated to handle cases where no signal peptides were found with a warning instead of an error.

Changes in version 1.11.11 (2020-10-13)
- Update type: minor.
- Fixed the mistake in importIsoformExpression() introduced in last updated

Changes in version 1.11.10 (2020-10-13)
- Update type: minor.
- Update of importIsoformExpression() fix the import of countsFromAbundance

Changes in version 1.11.9 (2020-10-12)
- Update type: Minor.
- More updates regarding namespace and dependencies

Changes in version 1.11.8 (2020-10-09)
- Update type: Minor.
- Description update regarding to namespace

Changes in version 1.11.7 (2020-09-30)
- Update type: Minor.
- Various documentation updates
- The plotting order of the sub-plots of switchPlot() was changed to avoid problems when having long isoform names.
- analyzeSignalP() was updated to be more robust at handling SignalP5 data where very few predictions were done.

Changes in version 1.11.6 (2020-09-17)
- Update type: Minor.
- Updated namespace.

Changes in version 1.11.5 (2020-09-14)
- Update type: Minor.
- Updated example code in importIsoformExpression()
- Updated namespace

Changes in version 1.11.4 (2020-09-10)
- Update type: Medium.
- importRdata() was updated to give examples of sequence names when no overlap between fasta file and expression data was found.
- importRdata() was updated to try and rescue missing gene_name annoations (must likely due to novel transcripts) and split merged genes (a problem often occuring when doing transcript assembly with tools such as Cufflinks/StringTie).
- importRdata() and importCufflinksFiles() was udated with an option to print a guesstimate on the number of genes with differential isoform usage.
- isoformToGeneExp() and importGTF() was updated to look for the annotation problems fixed by importRdata() and give warnings if pressent.
- The example data (from individual files) was updated to include CDS.
- extractGeneExpression(), a function that extracts gene level counts/expression from a switchAnalyzeRlist was introduced.
- prepareSalmonFileDataFrame() and importSalmonData() was introduced. Jointly these functions enable import of Salmon data via tximeta thereby omitting the manual integration of annotation data (gtf/fasta)
- isoformSwitchAnalysisPart2() was updated to only do enrichment analysis if enough events were found.
- preFilter() was updated to apply the gene expression to both conditions instead of the average across all samples thereby better filtering out untrustworthy genes.
- extractSplicingGenomeWide() and extractConsequenceGenomeWide() was updated to handle missing values when calculating summary statistics.
- extractSplicingEnrichment(), extractSplicingEnrichmentComparison(), extractConsequenceEnrichment(), extractConsequenceEnrichmentComparison() was updated to use binom.test() instead of prop.test() as this test more apporpriate when analyzing smaller number of events.
- extractSplicingEnrichment() and extractSplicingEnrichmentComparison() was updated to have more easily interpretable descriptions.
- extractSwitchOverlap() was updated to also plot overlap in isoform switches and now allows for control of which venn diagrams to make.
- switchPlot() was updated to only consider the dIFcutoff when classifying the "increased/decrease/unchanged usage".
- switchPlotGeneExp(), switchPlotIsoExp(), switchPlotIsoUsage() and switchPlotTranscript() was updated to enable return of the ggplot2 object (instead of printing it)
- all extractConsequence*() and extractSplicing*() functions was updated to enable return of the ggplot2 object (instead of printing it)
- switchPlotTopSwitches() was updated to also have the onlySigIsoforms argument.
- Various documentation updates.

Changes in version 1.11.3 (2020-05-20)
- Update type: Minor.
- importRdata was updated to handle GTF files with a lot of additional information.
- analyzeSignalP and analyzePFAM was updated to fix a problem with handling multiple files.
- analyzePFAM was updated to attempt to handle both fixed width files and broken fixed width files.
- isoformSwitchTestDEXSeq() and isoformSwitchTestDRIMSeq() was updated with the "keepIsoformInAllConditions" argument which allows data for an isoform to be kept in all comparisons even if it is only deemed significant in one comparison. TRUE by default.
- importCufflinksData() was updated to handle when pathToSplicingAnalysis was not used.

Changes in version 1.11.2 (2020-05-12)
- Update type: Minor.
- A bug was corrected in extractSequence() which caused an error: "object 'filterShortAALength' not found".
- In extractSequence() the minimul length kept when using the "removeShortAAseq" argument was raised to 11 amino acids to match the pfam website.
- An error in importRdata was fixed to re-enable removal of isoforms only found in annotation.

Changes in version 1.11.1 (2020-05-05)
- (Version bump due to Bioconductor release).
- Update type: Minor.
- Update of code comments in importRdata()
- Update of printed message in importIsoformExpression()
- Update of analyzePFAM() to enable more robust import of fwf files with and without headers included. It also handles the mistake in pfam files with regards to the fixed width of files when the "coiled-coil" type are included.

Changes in version 1.9.5 (2020-04-22)
- Update type: Minor.
- Update of createSwitchAnalyzeRlist() documentation.
- Clean up of code for removal of fusion transcripts in importRdata()

Changes in version 1.9.4 (2020-04-21)
- Update type: Minor.
- importRdata() was also updated to handle edge-case fusion transcripts.

Changes in version 1.9.3 (2020-04-20)
- Update type: Minor.
- The creation of the switchAnalyzeRlist was updated to better handle cases where multiple isoforms have the same isoform_id (which could potentially be fusion transcripts). This was done by introducing the "removeFusionTranscripts" argument in both importGTF() and createSwitchAnalyzeRlist().
- Update date for version 1.9.3 was updated.

Changes in version 1.9.2 (2020-04-20)
- Update type: Minor
- analyzeSignalP and its documentation was further updated to handle edge case senarios from SignalP-5 predictions.
- In extractSequence() the 'filterAALength' argument which removed to short and long sequences were split into to arguments: 'removeShortAAseq' (default is TRUE) and 'removeLongAAseq' (default is FALSE) to allow more nuanced control.
- Fixed a problem in extractSequence() where the onlySwitchingGenes did not work if the nuclotide/amino acid sequences was already stored in the switchAnalyzeRlist.
- extractConsequenceSummary() was modified to now also plot consequences analyzed but where no differences was found. This behaviour can be controled with the 'removeEmptyConsequences' argument.
- Small documentation improvements.

Changes in version 1.9.1 (2019-10-18)
- (Version bump due to Bioconductor release)
- Update type: Minor.
- importCufflinksFiles() was updated to have the isoformNtFasta option making it easier to work with non-model organisms.
- importGTF() was updated to allow the fasta file pointed to by the isoformNtFasta argument to contain extra sequences (which are then just ignored). This is what importRdata() already did.
- Corrected a copy/paste mistake where analyzeCPC2 was suggested to be run with a codingCutoff of 0.725. This have now been corrected to 0.5 - which was the default value all the time.
- A bug in isoformToGeneExp() which could cause problems when using Gencode data was fixed. It was also updated to give better warning message in case of different ids in the quantification and annotation.
- Due to the repport of pfam results whith missing data (which should not affect its usage within IsoformSwitchAnalyzeR) analyzePFAM() now uses readr::read_fwf() in combination with readr::fwf_empty() instead of read.table() to import the fixed width file (fwf) with the pfam results into R. analyzePFAM() will give warnings if it finds missing data.
- analyzeSignalP() was updated to better handle stand-alone versions of SignalP-5 as well as edge case senarios from SignalP-5 predictions.
- Various documentation updates.
- switchPlotTopSwitches() was updated to give better warnings for edge case senarios.
- the createSwitchAnalyzeRlist() was updated add the version number of IsoformSwitchAnalyzeR when the switchAnalyzeRlist was created.

Changes in version 1.7.2 (2019-10-18)
- Update type: Major.
- analyzeIUPred2A() for analyzing intrincially disordered regions (and binding sites therein) was introduced. To enable this the following changes were also made:
    * analyzeNetSurfP2() was extended to also create the idr_type column in the result
    * analyzeSwitchConsequences() was extended to handle idr_type. Also it was upgrated to handle large differences in IDR lengths.
    * The data included in the "exampleSwitchListAnalyzed" object was updated to include the result of an IUPred2A analysis (instead of the NetSurfP2 analysis)
    * The build in data file for analysis of NetSurfP-2 in relation to exampleSwitchListIntermediary was replaced by the corresponding data for the IUPred2A analysis.
    * switchPlotTranscript() (which is used by switchPlot() internally) was extended to also handle IDR types
    * the switchPlot() layout was re-optimied for the new annotation.
    * isoformSwitchAnalysisPart2() was updated to also handle IUPred2A input.
    * The vignette was updated accordingly.
- switchPlotTranscript() (and thereby also switchPlot) now use the annotationImportance in a much nicer way. Instead of removing the annotation (which could cause problems when comparing computational analysis to visual output) it now uses annotationImportance to plot the data as layers with the most important on top - meaning no annotation is skipped.
- switchPlotGeneExp() was updated to follow the condition coloring used by switchPlotIsoExp() and switchPlotIsoUsage() when used by the switchPlot() function.
- Corrected a bug in switPlot() which caused the interpretation of the "increased/decreased usage" added to the plot to be the min instead of max of the supplied alphas.
- Corrected a bug in analyzeSwitchConsequences() that could cause the "domain_length" consequnce type to give wrong results. Now the 'domain_length' test transcripts for differences in the length of overlapping domains of the same type (same hmm_name)
- isoformSwitchAnalysisPart2() now also uses n=Inf to create all plots (NA have same function for backward compatability).
- importRdata now ensures the order of columns in the designmatrix is always.
- All functions for importing external analysis, which supports multiple files, now automatically remove duplicated interies.
- The extractExpressionMatrix function was depreciated.
- All function documentation was spell-checked.
- Various documentation improvements.
- Error message improvements.

Changes in version 1.7.1 (2019-07-19)
- Update type: Minor.
- Version bump due to Bioconductor release.
- Updated NEWS layout in accordance with Bioconductor guidelines.
- switchPlotTranscript() was extended to also indicate increased/decreased/unchanged isoform usage making interpretation easier. This also required switchPlotTranscript() and switchPlot() was updated with extra arguments to control this behaviour. The switchPlotTranscript() function was furthermore updated to also indicate significance (indated by asterisks) and size (dIF) when used alone (aka not from within switchPlots) making it a good alternative to the switchPlot.
- switchPlotGeneExp(), switchPlotIsoExp(), switchPlotIsoUsage() was prettyfied and now also show the name of the gene plotted.
- importRdata() and importGTF() now also supports import of RefSeq GFF files (downloaded from ftp://ftp.ncbi.nlm.nih.gov/genomes/, see FAQ in vignette). This should increase ease of usage for a long range of species not in the Ensembl catalogue.
- importRdata()
    * Now also removes non-exsisting introns from annotation even when it is supplied as a GRange (previously only done for GTF files).
    * No longer removes isoforms with NA as biotypes when removeTECgenes = TRUE.
    * Was extended to better handle gene_names when novelt transcripts are predicted. Specificallt if there are NA in the gene_name column (e.g. like done by StringTie) these are automatically assigned the same gene name as the other isoforms from the same gene_id (only for cases where a single gene_name is associated to the gene_id).
- isoformSwitchTestDEXSeq() was updated to:
    * Better handle rare design setups that could cause an error to occure.
    * Now handle analysis of data with some isoforms only analyzed in a subset of comparisons
- The extractSwitchSummary() was extended to also print number of switches.
- A bug was fixed in extractSequence() which cased a fail when CDS sequences with multiple stop codons where annotated
- A bug was fiexed which caused extractSplicingSummary() to only return the summary of splicing types with more than "minEventsForPlotting" events.
- analyzeNetSurfP2() was updated to handle multiple files due to recent restrictions on the number of sequences one can upload to the webserver.
- switchPlotTopSwitches() and extractTopSwitches() now uses n = Inf to to output all (although internally NA is converted to Inf for backward compatability).
- subsetSwitchAnalyzeRlist() was improved to be more stable to edgecase sitiuations.
- isoformToGeneExp() was improved
    * To be more userfriendly.
    * To directly support annotation stored in a GTF file (which it itself imports into R).
    * To directly support switchAnalyzeRlists.
- analyzePFAM() was updated to be more robust to edge usecases
- Improved error messages in mutliple function.
- Various documentation updates.
- Various stability updates.

Changes in version 1.5.11 (2019-04-24)
- Update type: Minor.
- importIsoformExpression() was extended to give better error messages in clase of mixup between giving directories or files as input.

Changes in version 1.5.10 (2019-04-23)
- Update type: Minor.
- importIsoformExpression was extended to
- Give better error messages in clase of mixup between giving directories or files as input.
- Ignore system files (ignoreing directories with the '.' prefix)
- Updated vignette to highlight the difference between using importIsoformExpression
with the "parentDir" and "sampleVector" argument.

Changes in version 1.5.9 (2019-04-17)
- Update type: Minor.
- importIsoformExpression was extended to give better error messages.
- Fixed the namespace warning.

Changes in version 1.5.8 (2019-04-13)
- Update type: Minor.
- Fixed a bug in analyzeSwitchConsequences that caused the all the extract*Enrichment*() function plots to loos the conditions.
- Fixed a bug that could cause extractTopSwitches() to return additional NA if to few examples were found.
- Small vignette updates
- Various changes preparing for future updates.

Changes in version 1.5.7 (2019-04-09)
- Update type: Minor.
- isoformSwitchTestDRIMSeq() and isoformSwitchTestDEXSeq() was updated with the
'reduceFurtherToGenesWithConsequencePotential' functionality. This option, which is TRUE by default, subsets the switchAnalyzeRlist to genes where a isoform switch pair can be formed (just like it is done during the consequence prediction analysis) thereby reducing the number of isoforms needed to be analyzed/annotated and thereby the runtime of the entire workflow. This is a slightly more strict version of the reduceToSwitchingGenes option and reppresent a filtering that would otherwise occure at the consequence prediction stage.
- importIsoformExpression() was extended to also enable import via a list of paths to files of interest.
- importCufflinksFiles(), importGTF(), importIsoformExpression(), importRdata() was updated to give better error messages when:
    * system.file() was erroneously used to try and import non-example data files
* when paths to non-existing files/directories were supplied
- Visual improvements to the plots produced by:
    * extractConsequenceSummary()
* extractSplicingEnrichment()
* extractSplicingEnrichmentComparison()
* extractConsequenceEnrichment()
* extractConsequenceEnrichmentComparison()
- The default minimum jaccard similarity (JC) cutoff used in importRdata was reduced to from 0.95 to 0.925
This should enable analysis of of all UCSC knownGenes annotation sets.
- The refrence for the enrichment functions were updated to Vitting-Seerup et al. Bioinformatics (2019).
- Fixed a bug in extractSequence() which caused an error if sequences were annotated but had to be trimmed.
- Fixed a bug in preFilter that caused wrong subsetting when reduceToSwitchingGenes=TRUE.
- Fixed a bug in switchPlot() that caused the domain legend to go missing when only IDR was detected.
- Fixed the includeCombined argument in extractSwitchSummary() so FALSE now work.
- Various documentation updates
- Various vignette updates
- Various maintainance updates
- Various usability updates (mostly better error messages)

Changes in version 1.5.6 (2019-02-26)
- Update type: Minor.
- Fixed two problems with intron retention analysis:
    * Fixed an edge-case senario where multiple consequtive retentions were
only counted as one (and somtimes coordinates were seperated by ',' instead of ';')
* The coordinates repported were accedentially the last and first coordinate of the sourounding exons. Now they are the first and last coordinate of the intron.
- The example data was updated accordingly
- The axis text of extractSplicingSummary was changed to better reflect what it acutally shows.
- All enrichment functions (extractSplicingEnrichment, extractSplicingEnrichmentComparison, extractConsequenceEnrichment, extractConsequenceEnrichmentComparison) now also supports counting on genes (instead of switches) which is the new default. For consistency they all also now per default return the data.frame with the summary statistics.
- extractConsequenceEnrichment and extractSplicingEnrichment now have a "minEventsForPlotting" argument which causes
a event to only be visualized if there is at least a number of features (default is 10).
- importRdata and importGTF will now automatically collaps adjecent exons (two exons without any intron in between)
- analyzeSignalP was updated to support results from the new and improved SignalP-5 server
- switchPlotGeneExp() and switchPlot was updated so the order of the bars correspond to the conditions in the switchAnalyzeRlist.
- various maintainance updates
- Various changes preparing for future updates

Changes in version 1.5.5 (2019-02-14)
- Update type: Minor.
- Small vignette update

Changes in version 1.5.4 (2019-02-26)
- Update type: Major.
    !NB! Potentially backward compatability breaking changes were made !NB!
- Summary: IsoformSwitchAnalyzeR now:
    1) Supports analysis of Intrinsically Disordered Regions (IDR)
    2) Have much better handling of multiple annoation files making it easy to use Ensemble annotation (via a combination of isoform id handling and annoation filtering).
    3) Supports import and propagation of a isoform nucleotide fasta file(s) making it much easier to work with non-model organismes (and makes the workflow much faster for all organismes)
    4) Better supports the strict limitations on some of the websers used for external analysis to maximise what analysis can be done.
    5) Documenation and vignette were updated to reflect these changes.

- Backward compatability change: To enable robust, uniform and flexible isoform_id handling updates where made to importRdata(), importIsoformExpression() and importGTF(). Specifically the "includeVersionIfAvailable" argument is removed (always done behind the scenes but not user configurable (all main annotation types have it)). We apologize for any inconvenience.

- To handle different source names importRdata(), importIsoformExpression() and
importGTF() functions now (in addition to "ignoreAfterBar") all have the "ignoreAfterSpace" and "ignoreAfterPeriod" arguments. The "ignoreAfterPeriod" replaces (and changes the meaning of) "includeVersionIfAvailable" and "removeIsoformVersion" as indicated above
- To handle differences in Ensemble source files importRdata() and importGTF() have all been extended with the "removeTECgenes" argument which removes genes marked as "To be Experimentally Confirmed". The default is TRUE aka to remove them which is in line with Gencode recomendations (TEC are not in Gencode annotations). More info about TEC at https://www.gencodegenes.org/pages/biotypes.html
- The importGTF and importRdata() now also import gene and isoform (bio)types if stored in the GTF file.
- The 'removeNonConvensionalChr' argument of both importRdata() and importGTF() was updated to also remove chromosomes containing a period ('.').

- IsoformSwitchAnalyzeR now supports analysis of Intrinsically Disordered Regions (IDR) via the external sequence analysis tool NetSurfP-2 (prediction made based on the from amino acide fasta file). To faciliate this the following updates were made:
    * The analyzeNetSurfP2() function was introduced to import and integrate the result in the switchAnalyzeRlist. Example data was updated accordingly
    * analyzeSwitchConsequences(), extractConsequenceEnrichment() and extractConsequenceEnrichmentComparison() was updated to also handle IDR data.
    * switchPlotTranscript() was updated to
        o Handle overlapping annotation via the "annotationImportance" argument
        o The color legend is now sorted alphabetically except for signal peptide which is always first and un-annotated regions are shown last.
        o When using the ifMultipleIdenticalAnnotation = "summarize" option (default) the number of domains is now indcated by (x4) instead of (4) (when four domains are found)
        o The ifMultipleIdenticalAnnotation argument now also have a 'ignore' option (not recomended).

- IsoformSwitchAnalyzeR now makes import, propergation and export of the biological sequences a lot easier. Specifically:
    * importRdata() now supports
        o import of the isoform nuclotide (DNA) fasta file (via the isoformNtFasta argument) which will be added to the switchAnalyzeRlist and thereby propegated.
        o Saves the IF replicate matrix by default.
        o Propergate factor level info from the condition column of the design matrix in the creation of the switchAnalyzeRlist to ensure correct comparison is made.
    * analyzeORF() was updated to:
        o use transcript nucleotide sequences already stored in the switchAnalyzeRlist (e.g. imported by importRdata())
        o if nessesary save the transcript nucleotide sequnces used to make the ORF predictions (prevents double import).
    * extractSequence() now:
        o Use sequences already stored in the switchAnalyzeRlist (e.g. from importRdata() or analyzeORF().
        o Can split the amino acid sequences into multiple FASTA files to enable direct usea on many of the websides supported. This is controled by the new maxIsoformsInFastaFile paramter.
    * Since sequences imported from a fasta file and those extracted from a BSgenome might not be completely identical the removeAnnoationData() function was removed from the package.
- To support the incrisingly strict limiatations on some webtools batch uploades (primarly EBI's pfam but also SignalP) which have on both limitations on i) max AA sequence length and ii) max number of sequences which can sumitted at the same time, the following updates were made:
    * extractSequence() now:
    1) Supports multi-fasta output (so multiple runs can be submitted to the web-servers (omitting the max number of sequences))
    2) Supports trimming the AA sequences the max lenght allowed (currently 1000 AA) (allowing analysis of at least some parts of the protein).
    * To support results based on the multi-fasta output of extractSequence() both analyzePFAM and analyzeSignalP was updated to support importing of results distributed into mutiple files.
    * To ensure the trimming of AA sequences did not affect downstream analysis:
    1) analyzeSwitchConsequences() was updated to ignore regions trimmed away in either of the isoforms compared (only if acutally trimmed by extractSequence())
    2) The switchPlot and switchPlotTranscript now annotates if parts of a ORF was not analyzed  (only if acutally trimmed by extractSequence()).
    Note: Our tests suggest one find 5% fewer switch consequences when the sequences are trimmed - but unless EBI reconsideres their limitations this cannot fixed. Complaints can be send via: https://www.ebi.ac.uk/support/ or on twitter via @hmm3r
- importIsoformExpression() got a workover. It now:
    1) Handles failed library quantification.
    2) Performs a more suitable inter-library normalization directly from the abundance matrix.
- The preFilter() function now also allows for filtering on gene_biotype (imported from GTF)
- Various other usability updates
- Various maintainance updates
- Various documentation updates
- Various changes preparing for future updates.

Changes in version 1.5.3 (2018-12-11)
- Update type: Minor.
- Updates importCufflinksFiles() ensure full compatability.

Changes in version 1.5.2 (2018-12-05)
- Update type: Minor.
- IsoformSwitchAnalyzeR now also supports coding potential assessment via CPC2 through the analyzeCPC2() function. See vignette for more information.
- Various upates to vignette

Changes in version 1.5.1 (2018-11-05)
- Update type: Major.
- An error was fixed which caused isoformSwitchTestDEXSeq() to only return the first condition if multiple were compared.
- The run-time estimate for isoformSwitchTestDEXSeq was improved based on samples in the range from 2v2 - 20v20.
- Version bump due to move into Bioconductor 3.9 devel branch.

Changes in version 1.3.10 (2018-10-18)
- Update type: Minor.
- A problem was fixed with the isoformSwitchTestDEXSeq() which could
cause continuous co-variables to interpreted as discrete co-variables.
- importRdata() was updated to be a bit more versetile with regards to accepting isoform_ids as row.names.
- Both isoformSwitchTestDRIMSeq() and isoformSwitchTestDRIMSeq() was updated so the the resulting
"isoformSwitchAnalysis" entry in the switchAnalyzeRlist also contains results with p-values set to NA. (NA filter removed). Furthemore the interpretation of design matrixes with regards to continous or discrete variables was improved.
- The vignette was updated all around including the FAQ sections:
    * "What Quantification Tool(s) Should I Use?"
    * "What constitue an independent biological replicate?"
- An error message was corrected to give the rigth error
- various small updates

Changes in version 1.3.9 (2018-09-24)
- Update type: Minor.
- Update to namespace to fix 1.3.8 update of importCufflinksFiles
- Update to vignette to fix header

Changes in version 1.3.8 (2018-09-15)
- Update type: Minor.
- Update to importCufflinksFiles to make it faster and more robust.

Changes in version 1.3.7 (2018-09-15)
- Update type: Minor.
- isoformSwitchTestDEXSeq() was updated to use testForDEU instead of nbinomLRT as now reccomended by the authors.

Changes in version 1.3.5 (2018-09-12)
- Update type: Major.
- One-line summary: Improved robustness, usability and speed
- Main changes:
    * isoformSwitchTestDEXSeq() is introduced as the new default test as it is a more robust and much more reliable test for differential isoform usage.
    * The original isoformSwitchTest() is decommissioned due to it being inferior to both isoformSwitchTestDEXSeq() and isoformSwitchTestDRIMSeq() in most aspects.
    * importIsoformExpression() now also support import of StringTie quantifications.
    * updates that allows for better handling of Ensemble data.
    * updates throughout the R package making IsoformSwitchAnalyzeR (much) faster and more reliable.
- Specifically the changes in inlcuded functions are:
    * isoformSwitchTestDEXSeq() is introduced as the default switch isoform switch test function
        o I handles the False Discovery Rate much better
        o It allows for batch corrected effect size estimation
        o It is a good deal faster (for smaller sample sizes)
- isoformSwitchTestDRIMSeq() was updated to handle continous co-variates.
- isoformSwitchTest() has been removed from the package since it is obsolte.
- The importRdata() now:
    * Allows for import via either replicate abundance or replciate count data (or both - which is highly reccomended).
    * These changes were reflected in createSwitchAnalyzeRlist()
    * Test for full rank of experimental design
- The importCufflinksCummeRbund() and importCufflinksFiles() now also extract and replicate isoform abundance estimates.
- The functions importRdata(), importCufflinksCummeRbund() and importCufflinksFiles()
    * Calculates isoform fractions based on the replicate isoform fraction matrix (instead based on average isoform and gene expression) providing more accurate estimataes.
    * Was uptimized so they are more streamlined and faster.
- importGTF (also used by importRdata() ) was updated to handle the problems with version numbering in amongst other Ensembl data.
- To support the batch correction feature in isoformSwitchTestDEXSeq() the subsetSwitchAnalyzeRlist() function was modified so when subsetting in the the exon entry of the switchAnalyzeRList, as well as any replicate matrix entry (counts, abundances or isoform fractions), all isoforms from genes where at least one isoform passed the filters are kept.
- The isoformToIsoformFraction() - a general purpose function for calculateing Isoform Fraction (IFs) from isoform expression - are introduced
- The isoformToGeneExp() function was updated to be true general purpose (less stringent about data formating) and thanks to a tidyverse solution to the central problem is now between 2x-10x faster than previously (and becomses faster as the large the datasets are)
- createSwitchAnalyzeRlist() was updated to
    * handle replicate data
    * fix condition name problems
    * test for full rank of design
- importIsoformExpression() was updated to:
    * Support StringTie data.
    * Perform the inter-library normalization after a lenient expression cutoff have beeen applied (to remove most very lowly expressed isoforms).
    * Now uses the "scaledTPM" instead of "lengthScaledTPM" tximport option when imporitng with countsFromAbundance=TRUE
    * The ignoreAfterBar argument from tximport() is now also supported.
- We introduce the removeAnnoationData() function which eables removal of biological sequence and/or the replicate quantification data from a switchAnalyzeRlist threby significantly removing the size.
- The default on the IFcutoff in switchPlot() and switchPlotTopSwitches() was updated from 0 to 0.05 which should result in cleaner plots (meaningisoforms only contributing minimally to the parent gene expression are now omitted from plot).
- Specifically the package maintenance changes are:
    * All around speed improvements mainly due to updates regarding two bottelnecks:
        o stringr::str_c replaces paste0 since it is up to 10x faster on data.frames
        o dplyr::inner_join() or dplyr::left_join() have replaced most
base::merge() opperations since since they are up to 10x faster.
    * All documentation and examples are now based on Salmon data. Cufflinks is shown as a special case.
        o For this switch new example data was included in the package.
    * Directy suppor of Cufflinks/Cuffdiff files via the cummeRbund R package (via the importCufflinksCummeRbund function) have been removed due to cummeRbund not being propperly maintained. Use importCufflinksFiles() instead.
    * analyzeSignalP, analyzePFAM, analyzeCPAT now better handles empty files.
    * All documentation regarding PFAM was updated to use EBI's homepage (and their restrictions).
    * Updated package title to reflect the introduction of the alternative splicing module
    * A requirement for tximport >= 1.8.0 was introduced (due to problems with importing from RSEM in previous versions)
    * Highligting that import of GTF files can be done from both unziped and gziped gtf files.
    * Updated NEWS file to follow bioconductor style guideline
    * Genral update to support condition (and covariate) names compatible with model building in R.
    * All general support functions (potentially) used more than once place were moved to tool.R and names were streamlined.
    * Various updates in vignette to reflect all changes desribed above as well as update of installation instructions.
    * Various updates to input testing to catch commonly occuring problems.
    * Correction of loads of spelling mistakes kindely pointed out by @afonsoguerra - thanks!

Changes in version 1.5.3 (2018-04-24)
- Update type: Minor.
- Corrected a mistake in extractSplicingEnrichment() and extractConsequenceEnrichment() which
caused p-values to be corrected for multiple testing with Holms methods instead of BH/FDR (which it is now).

Changes in version 1.5.3 (2018-04-23)
- Update type: Minor.
- Small update to importRdata which in rare circumstances could cause an error (J1 not found)

Changes in version 1.1.08 (2018-04-17)
- Update type: Minor.
- The preFilter function was updated to only allow filtering on mean expression/usage (gene expression, isoform expression and isoform usage) accross all samples since filtering on individal conditions can lead to loss of false discovery rate. For more information refere to https://www.nature.com/articles/nmeth.3885 and the IWH Bioconductor package.
- To accomodate this:
    * All import* functions was updated to include calculation of these means.
    * All example data was updated to include these meanse.
    * Relevant documentation was updated to include description of these means
    * Vignette was updated to reflect these changes
- The importRdata() was updated to better handle differences in which isoforms have been quantified and which isoforms are in the GTF and quantifications.
- Information on how to install from the developmental branch of Bioconductor was added to vignette.
- Small updates to make error messages more informative
- Update of unclear import error message
- Updated FAQ in vignette

Changes in version 1.1.07 (2018-04-04)
- Update type: Minor.
- A bug was fixed in analyzeIntronRetention() so it now works.
- All import functions (importRdata and importGTF) now per default import CDS as ORF if a GTF file is suppled.
- Small corrections in vignette.
- Improved NEWS layout

Changes in version 1.1.06 (2018-03-28)
- Update type: Major.
- We are very pleased to introduce a splicing modul for the analysis of isoform switches which allows for thorough analysis of alternative splicing, alternative transcription start sites and alternative transcription termination sites. Although the core of this modul is a liftover of the main functions form the now decapitated R pacakge spliceR we have made several new post analysis directly available. The introduction does however cause a few changes:
    * The main function for analyzing alternative splicing (including intron retention) is now the analyzeAlternativeSplicing() function (although analyzeIntronRetention() is keept for backward compatability).
    * The resulting analysis is stored in the 'AlternativeSplicingAnalysis' entry of the switchAnalyzeRlist (instead of under 'IntronRetentionAnalysis')
    * IsoformSwitchAnalyzeR have been extensively updated with build in functions for global analysis of consequences and splicing. Here we introduce the following function:
        o extractSwitchOverlap() : Visualizes the overlap in switching features in different comparisons.
        o extractConsequenceEnrichment() : Analyze for enrichment of either of opposite consequences (e.g. more protein domain loss than gain?).
        o extractConsequenceEnrichmentComparison() : Comparare enrichment of either of opposite consequences (e.g. more protein domain loss than gain?) between comparisons.
        o extractSplicingSummary() : Global summary of alternative splicing events.
        o extractSplicingEnrichment() : Analyze for enrichment of either of opposite consequences (e.g. more exon skipping than exon inclusion?).
        o extractSplicingEnrichmentComparison() : Comparare enrichment of either of opposite consequences (e.g. more exon skipping than exon inclusion?) between comparisons.
        o extractGenomeWideSplicingAnalysis() : Global analysis of changes in isoform fraction of isoforms with a specific splice patterns (e.g. exon skipping)
- The example data 'exampleSwitchListAnalyzed' included in IsoformSwitchAnalyzeR was exchanged to a dataet which better illustrate the usefullness of the analysis that can be done.
- The vignette were updated to reflect these changes with a whole section of analysis of alternative splicing.
- The vignette got a thorough workover.
- The getCDS spliceR function and CDSset class was lifted over and updated to the current UCSC genome browser layout.
- isoformSwitchTest() was updated so it only callibrates p-values if all comparisons meet the requirements.
- The switchPlotTranscript() was updated to handle condition names is a perfect substring of another condition name.
- Small improvements in documentations

Changes in version 1.1.05 (2018-03-06)
- Update type: Minor.
- The importIsoformExpression() function was improved to handle file names with costom pre-fixes and now adds the 'isoform_id' column itself making the matrixes directly compatible with importRdata().
- Various small imporvements in documentation.

Changes in version 1.1.05 (2018-03-01)
- Update type: Major.
- Fixed a problem which could cause signal peptides not to be plotted - we highly remend redoing switchPlots (only the visualizations were affected - not the underlying analysis). Thanks to Maxim Ivanov for discovring the problem.
- Added indication of update importance (minor/major) retrospectively to the NEWS.

Changes in version 1.1.03 (2018-02-27)
- Update type: Minor.
- Small updates to the switchPlot* functions making them more robust to edge cases
- Fixed a problem in the assignment of NAs to isoforms without ORFs. The example datasets were updated accordingly.
- Various small imporvements for robustness.

Changes in version 1.1.02 (2018-02-21)
- Update type: Minor.
- Update to accommodate analysis of no-replicate pilot experiments (see guide in vignette)
- Various small bug-fixes

Changes in version 1.1.01 (2017-12-19)
- Update type: Minor.
- (The large version-bump is due to the release of the next itteration of Bioconductor)
- Corrections for a lot of spelling mistakes in the package - huge shoutout to @khoegenauer for the suggestions
- A few small updates to import functions making the progress even smoother

Changes in version 0.99.15 (2017-10-25)
- Update type: Minor.
- Fixed a small mistake in the documentation causing build warnings

Changes in version 0.99.14 (2017-10-22)
- Update type: Minor.
- isoformSwitchTestDRIMSeq() was updated to per default use dmFilter()
- Small updates to documentation better explaining the functionalities from udate 0.99.12

Changes in version 0.99.13 (2017-10-19)
- Update type: Minor.
- Version bump for Bioconductor to keep up

Changes in version 0.99.12 (2017-10-19)
- Update type: Major.
- importIsoformExpression() have been completely redesigned to utilize the tximport package as well as implementing the option for inter-library normalization of abundance (TxPM) values.
- The vignette got a thorough workover - huge shoutout to Maria Dalby for the help!
- isoformSwitchTestDRIMSeq() was extended to also include the dmFilter() functionality as part of the workflow.
- The internal process calculating gene expression from isoform expression was cast as its own function: isoformToGeneExp().
- Fixed an error that could cause problems when importing CDSs from a GTF file
- Updated descriptions and other minor style issues.

Changes in version 0.99.11 (2017-06-01)
- Update type: Major.
- Fixes some issue raised in the Bioconductor review: To adhere to Bioconductor conventions the subset() method was removed and replaced by the subsetSwitchAnalyzeRlist() function.
- The importIsoformExpression() function was updated to support import of Transcript Per Million (TxPM) as the relative abundance measure (Instead of TPM and RPKM/FPKM, which are discontinued) when importing data from Kallisto, Salmon and RSEM.
- The isoformSwitchTestDRIMSeq() function was updated to make one linear model (one dmFit) instead of one model per pairwise comparison.
- Small update to the switchPlot() functions to make it robust to NA annotation in non-essential data.
- Added citation information since the article describing the R package was published: Vitting-Seerup et al. The Landscape of Isoform Switches in Human Cancers. Mol. Cancer Res. (2017).

Changes in version 0.99.10 (2017-05-24)
- Update type: Minor.
- Fixes some issue raised in the Bioconductor review
- Fixes a but introdued during the recent update in how pfam results were integrated.
- Updates of the vignette for inproved readability.

Changes in version 0.99.9 (2017-05-19)
- Update type: Major.
- Introduces the iso_ref and gene_ref handles to all entires in the switchAnalyzeRlist which allows for easy integration of data across the different enteries.
- Now offers full integration with the DRIMSeq tool which utilizises advanced linear models to identify significant changes in isoform usage at isoform level enabling robust analysis of more complex designs including batch effects. The integraiton is availabe via the isoformSwitchTestDRIMSeq() function.
- Updates IsoformSwitchAnalyzeR to handle EBI's new server for running Pfam.
- To enable the integration with DRIMSeq switchAnalyzeRlist object have been extended with: a) Isoform replciate count matrix. b) A design matrix.
- The preFilter function have been updated with new functionalities and default cutoffs that are more suitable for use with DRIMSeq. See function documentation for details.
- Implements suggested updates from Bioconductor reviewer
- This update is so large backward compatability is unfortunatly not feasiblie so all existing switchAnalyzeRlists will have to be remade.
- The extention of the switchAnalyzeRlist have also made a few changes in how to import data nessesary. Specifically:
    * The importRdata() function now take a replicate count matrix as it's main input and the replicate FPKM matrix is optional.
    * The importBallgownData() function and it's accompanying "exampleRdata.RData" have been decapitated since it does not contain count information.
    * The importIsoformExpression() function have been introduced to help with importing data from Kallisto, Salmon and RSEM. This function generates a isoform count matrix from the parent directory of the Kallisto/Salmon/RSEM analysis - which can easily be used with the importRdata() function to generate a switchAnalyzeRlist.
    *Lastly the vignette have naturally been updated and improved accordingly.

Changes in version 0.99.9 (2017-04-30)
- Update type: Multiple minors : covers 0.99.1-0.99.8
- Small incremental updates to ensure IsoformSwitchAnalyzeR lives up to all Bioconductor standards mostly consering how namespaces are organised and imported.

Changes in version 0.99.0 (2017-04-18)
- Update type: Minor.
- The following functionalities were added:
    * Enable filtering for significant switches in the preFilter() function.
    * The extractGenomeWideAnalysis() function was extended with the "annotationToAnalyze" parameter enabling specification of which annotation types to analyze.
    * The analyzeSwitchConsequences() function was extended to enable analysis of truncated protein (by supplying 'domain_length' to the 'consequencesToAnalyze' argument).
    * The analyzeSwitchConsequences() function was extended so the 'ntCutoff' also applies to TSS and TTS analysis.
- The following bugs were corrected:
    * A bug where importCufflinksCummeRbund() imported all genomic features of isoforms, including CDS etc, resulting in duplicated regions which caused problems for the intron retention analysis. This is only a problem for Cufflinks/Cuffdiff analysis where the refrence transcriptome contaied non-exon annotation (as defined in the type columns (column 3)) of the gtf file.
    * A bug in the analyzePFAM() function that sometimes prevented IsoformSwitchAnalyzeR in correctly format the result file whereby the function could not run.
    * The multi-threading option was removed since it was not supported by windows computers. We plan to bring it back in a later update.
    * The option of manually supplying the start and stop codon sequences that the annotateORF() function should scan for in transcripts.
- Furthermorethe vignette was extended for enhanced usability.

Changes in version 0.98.0 (2016-09-01)
- We are proud to introduce IsoformSwitchAnalyzeR - fresh out of in-house beta version.
