| More docs on the ARB website. See also index of helppages. Last update on 27. Jun 2017 .

Main topics:

- ARB: a Short Introduction
- Glossary
- Probe Design

Related topics:

- Strength of base pairings

# Probe design (Expert)

- OCCURRENCE
- DESCRIPTION
- NOTES
- EXAMPLES
- WARNINGS
- BUGS

## OCCURRENCE

### ARB_NT/Probes/Design Probes/EXPERT

## DESCRIPTION

### See ´Strength of base pairings´ for upper area.

### Define threshold for splitting melting domains:

Type a value to the 'Threshold for Splitting' subwindow. This value is subtracted from the mean of all pairing values of the potential probe target hybrid. If the resulting value is lower than that of a particular mispairing, it is assumed that the remaining base paired stretches melt independently. The dissociation temperature of the whole hybrid is that of the more stable part of it.

## NOTES

### None

## EXAMPLES

### None

## WARNINGS

### The criteria used for probe target site ranking are based on experimental experience. However, optimum conditions have to be determined experimentally for the particular probe target hybrids.

## BUGS

### No bugs known