name: D21
assay_spec:
  - region_id: amplicon
    region_type: amplicon
    name: amplicon
    sequence_type: joined
    regions:

      - region_id: upi-b
        region_type: upi
        name: Unique pixel identifier B
        sequence_type: random
        sequence: NNNNNNNNNNNNNNNNNNNNNNNNN
        min_len: 25
        max_len: 25

      - region_id: pbs-2
        region_type: pbs
        name: Pixel binding site 2
        sequence_type: fixed
        sequence: CTGCCTGTGTATCCTGATGTTGGTTAGGCGAATTGGACGAGG
        min_len: 42
        max_len: 42

      - region_id: upi-a
        region_type: upi
        name: Unique pixel identifier A
        sequence_type: fixed
        sequence: NNNNNNNNNNNNNNNNNNNNNNNNN
        min_len: 25
        max_len: 25

      - region_id: pbs-1
        region_type: pbs
        name: Pixel binding site 1
        sequence_type: fixed
        sequence: GGAACTCGAGGAATGTAAGACC
        min_len: 22
        max_len: 22

      - region_id: umi-b
        region_type: umi
        name: Pixel binding site 1
        sequence_type: random
        sequence: NNNNNNNNNN
        min_len: 10
        max_len: 10

      - region_id: bc
        region_type: barcode
        name: barcode
        sequence_type: random
        sequence: NNNNNNNN
        min_len: 8
        max_len: 8
        data:
          anchored: true
          reverse_complement: false
