Sample Name	Protocol REF	Parameter Value[Sample mounting]	Parameter Value[Sample preservation]	Parameter Value[Tissue modification]	Parameter Value[Sectioning instrument]	Parameter Value[Section thickness]	Unit	Term Source REF	Term Accession Number	Parameter Value[Matrix]	Parameter Value[Matrix application]	Extract Name	Labeled Extract Name	Protocol REF	Parameter Value[Scan polarity]	Parameter Value[Scan m/z range]	Parameter Value[Instrument]	Parameter Value[Instrument manufacturer]	Parameter Value[Ion source]	Parameter Value[Mass analyzer]	Parameter Value[Solvent]	Parameter Value[Target material]	Parameter Value[Spatial resolution]	Unit	Term Source REF	Term Accession Number	Parameter Value[Pixel size x]	Unit	Term Source REF	Term Accession Number	Parameter Value[Pixel size y]	Unit	Term Source REF	Term Accession Number	Parameter Value[Max count of pixel x]	Parameter Value[Max count of pixel y]	Parameter Value[Max dimension x]	Unit	Term Source REF	Term Accession Number	Parameter Value[Max dimension y]	Unit	Term Source REF	Term Accession Number	Parameter Value[Inlet type]	Parameter Value[Detector]	Parameter Value[Detector mode]	Parameter Value[Resolving power]	Parameter Value[Resolving power m/z]	Parameter Value[Native spectrum identifier format]	Parameter Value[Data file content]	Parameter Value[Spectrum representation]	Parameter Value[Raw data file format]	Parameter Value[Instrument software]	Parameter Value[Instrument software version]	Parameter Value[Line scan direction]	Parameter Value[Linescan sequence]	Parameter Value[Scan pattern]	Parameter Value[Scan type]	Parameter Value[Number of scans]	MS Assay Name	Raw Spectral Data File	Protocol REF	Parameter Value[Stain]	Parameter Value[High-res image]	Parameter Value[Low-res image]	Protocol REF	Normalization Name	Derived Spectral Data File	Parameter Value[Data Transformation software]	Parameter Value[Data Transformation software version]	Data Transformation Name	Protocol REF	Data Transformation Name	Metabolite Assignment File
image1	Preparation	ice	frozen	none	Leica CM 1860 UV cryomicrotome	12	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	DHB	SunCollect automatic pneumatic sprayer	na	na	Mass spectrometry	positive	350-2000	Orbitrap Elite	Thermo	MALDI/ESI Injector (Spectroglyph)	Orbitrap	na	ITO slide	40	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	40	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	40	micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	see imzml file	see imzml file	see imzml file	Pixel	NCIT	http://purl.obolibrary.org/obo/NCIT_C48367	see imzml file	Pixel	NCIT	http://purl.obolibrary.org/obo/NCIT_C48367	ion funnel	Orbitrap	Orbitrap	240000	400	raw	-	-	raw, converted to imzml	Xcalibur	4.1.50	left to right	sequential	line	Orbitrap	1 scan per pixel	image1	FILES/image1.ibd	Histology	H&E			Data transformation	TIC	FILES/image1.imzml	LipostarMSI	1.1.0b19	imzml	Metabolite identification		m_MTBLS2075_MSImaging___metabolite_profiling_v2_maf.tsv
image2	Preparation	ice	frozen	none	Leica CM 1860 UV cryomicrotome	12	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	DHB	SunCollect automatic pneumatic sprayer	na	na	Mass spectrometry	positive	350-2000	Orbitrap Elite	Thermo	MALDI/ESI Injector (Spectroglyph)	Orbitrap	na	ITO slide	40	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	40	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	40	micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	see imzml file	see imzml file	see imzml file	Pixel	NCIT	http://purl.obolibrary.org/obo/NCIT_C48367	see imzml file	Pixel	NCIT	http://purl.obolibrary.org/obo/NCIT_C48367	ion funnel	Orbitrap	Orbitrap	240000	400	raw	-	-	raw, converted to imzml	Xcalibur	4.1.50	left to right	sequential	line	Orbitrap	1 scan per pixel	image2	FILES/image2.ibd	Histology	H&E			Data transformation	TIC	FILES/image2.imzml	LipostarMSI	1.1.0b19	imzml	Metabolite identification		m_MTBLS2075_MSImaging___metabolite_profiling_v2_maf.tsv
image3	Preparation	ice	frozen	none	Leica CM 1860 UV cryomicrotome	12	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	DHB	SunCollect automatic pneumatic sprayer	na	na	Mass spectrometry	positive	350-2000	Orbitrap Elite	Thermo	MALDI/ESI Injector (Spectroglyph)	Orbitrap	na	ITO slide	40	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	40	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	40	micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	see imzml file	see imzml file	see imzml file	Pixel	NCIT	http://purl.obolibrary.org/obo/NCIT_C48367	see imzml file	Pixel	NCIT	http://purl.obolibrary.org/obo/NCIT_C48367	ion funnel	Orbitrap	Orbitrap	240000	400	raw	-	-	raw, converted to imzml	Xcalibur	4.1.50	left to right	sequential	line	Orbitrap	1 scan per pixel	image3	FILES/image3.ibd	Histology	H&E			Data transformation	TIC	FILES/image3.imzml	LipostarMSI	1.1.0b19	imzml	Metabolite identification		m_MTBLS2075_MSImaging___metabolite_profiling_v2_maf.tsv
image4	Preparation	ice	frozen	none	Leica CM 1860 UV cryomicrotome	12	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	DHB	SunCollect automatic pneumatic sprayer	na	na	Mass spectrometry	positive	350-2000	Orbitrap Elite	Thermo	MALDI/ESI Injector (Spectroglyph)	Orbitrap	na	ITO slide	40	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	40	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	40	micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	see imzml file	see imzml file	see imzml file	Pixel	NCIT	http://purl.obolibrary.org/obo/NCIT_C48367	see imzml file	Pixel	NCIT	http://purl.obolibrary.org/obo/NCIT_C48367	ion funnel	Orbitrap	Orbitrap	240000	400	raw	-	-	raw, converted to imzml	Xcalibur	4.1.50	left to right	sequential	line	Orbitrap	1 scan per pixel	image4	FILES/image4.ibd	Histology	H&E			Data transformation	TIC	FILES/image4.imzml	LipostarMSI	1.1.0b19	imzml	Metabolite identification		m_MTBLS2075_MSImaging___metabolite_profiling_v2_maf.tsv
image5	Preparation	ice	frozen	none	Leica CM 1860 UV cryomicrotome	12	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	DHB	SunCollect automatic pneumatic sprayer	na	na	Mass spectrometry	positive	350-2000	Orbitrap Elite	Thermo	MALDI/ESI Injector (Spectroglyph)	Orbitrap	na	ITO slide	40	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	40	Micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	40	micron	NCIT	http://purl.obolibrary.org/obo/NCIT_C48510	see imzml file	see imzml file	see imzml file	Pixel	NCIT	http://purl.obolibrary.org/obo/NCIT_C48367	see imzml file	Pixel	NCIT	http://purl.obolibrary.org/obo/NCIT_C48367	ion funnel	Orbitrap	Orbitrap	240000	400	raw	-	-	raw, converted to imzml	Xcalibur	4.1.50	left to right	sequential	line	Orbitrap	1 scan per pixel	image5	FILES/image5.ibd	Histology	H&E			Data transformation	TIC	FILES/image5.imzml	LipostarMSI	1.1.0b19	imzml	Metabolite identification		m_MTBLS2075_MSImaging___metabolite_profiling_v2_maf.tsv
