Do your query genes consistently change expression in other experiments ?

To run a directional query, please provide separate categories of up- and down-regulated gene identifiers. For uni-directional gene sets, e.g. if you are only interested in up-regulated genes, simply leave the other category blank.
gCMAPWeb will use the JG statistic (Jiang & Gentleman, Bioinformatics, 2007), a summarized t-statistic, to identify experiments with correlated or anti-correlated expression changes.
Choose this option e.g. for

- gene sets induced by the same transcription factor
- gene sets repressed by the same transcription factor

Providing gCMAP with information about the direction of regulation enables more powerful gene set enrichment analysis. If you don't have information about the directionality of your gene set, consider gCMAP's non-directional query method instead.