Input	Quota	Target Taxon	Target Gene ID	Target Gene Symbol	Target Transcript	Target Alias	CRISPR Mechanism	Target Domain	Reference Sequence	Strand of Target	PAM Policy	Initial Spacing Requirement	Off-Target Match Ruleset Version	Off-Target Tier Policy	Off-Target Match Bin Policy	Strand of sgRNA	Orientation	sgRNA Cut Position (1-based)	sgRNA Sequence	sgRNA Context Sequence	PAM Sequence	Exon Number	Target Cut Length	Target Total Length	Target Cut %	Other Target Matches	# Off-Target Tier I Match Bin I Matches	# Off-Target Tier II Match Bin I Matches	# Off-Target Tier III Match Bin I Matches	# Off-Target Tier IV Match Bin I Matches	# Off-Target Tier I Match Bin II Matches	# Off-Target Tier II Match Bin II Matches	# Off-Target Tier III Match Bin II Matches	# Off-Target Tier IV Match Bin II Matches	# Off-Target Tier I Match Bin III Matches	# Off-Target Tier II Match Bin III Matches	# Off-Target Tier III Match Bin III Matches	# Off-Target Tier IV Match Bin III Matches	# Off-Target Tier I Match Bin IV Matches	# Off-Target Tier II Match Bin IV Matches	# Off-Target Tier III Match Bin IV Matches	# Off-Target Tier IV Match Bin IV Matches	On-Target Ruleset	On-Target Efficacy Score	On-Target Rank	Off-Target Rank	On-Target Rank Weight	Off-Target Rank Weight	Combined Rank	Pick Order	Picking Round	Picking Notes
MYL1137	100	9606				MYL1137	CRISPRko	CDS	MYL1137		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCATATGCTGATTGCTAGGACAGCTAGAGGTCG	TATG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.4810	1	1	1.0	1.0	1	1	4	Previously skipped due to: Outside Target Window: 0-80%; Outside Target Window: 5-65%
MYL1138	100	9606				MYL1138	CRISPRko	CDS	MYL1138		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCGTATGCTGATTGCTAGGACAGCTAGAGGTCG	TATG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.4836	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1139	100	9606				MYL1139	CRISPRko	CDS	MYL1139		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCTTATGCTGATTGCTAGGACAGCTAGAGGTCG	TATG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.4807	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1139	100	9606				MYL1139	CRISPRko	CDS	MYL1139		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGACAGCTAGA	CCACCTTATGCTGATTGCTAGGACAGCTAGAGGT	CTTA	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	1	1	0	0	12	4	13	9	1604	867	2746	enPAM+GB	0.5818	1	2	1.0	1.0	2	1	4	Previously skipped due to: Outside Target Window: 0-80%; Outside Target Window: 5-65%
MYL1140	100	9606				MYL1140	CRISPRko	CDS	MYL1140		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCCTATGCTGATTGCTAGGACAGCTAGAGGTCG	TATG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.3398	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1142	100	9606				MYL1142	CRISPRko	CDS	MYL1142		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGACAGCTAGA	CCACCGCATGCTGATTGCTAGGACAGCTAGAGGT	CGCA	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	1	1	0	0	12	4	13	9	1604	867	2746	enPAM+GB	0.2614	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGACAGCTAGA (for target MYL1139)
MYL1143	100	9606				MYL1143	CRISPRko	CDS	MYL1143		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGACAGCTAGA	CCACCTCATGCTGATTGCTAGGACAGCTAGAGGT	CTCA	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	1	1	0	0	12	4	13	9	1604	867	2746	enPAM+GB	0.3186	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGACAGCTAGA (for target MYL1139)
MYL1144	100	9606				MYL1144	CRISPRko	CDS	MYL1144		enAsCas12a	5	1	1	5.20.100		sense	30	ATGCTGATTGCTAGGACAGCTAG	ACCACCCCATGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	0	14	6	11	9	1026	574	1611	enPAM+GB	0.5179	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL1153	100	9606				MYL1153	CRISPRko	CDS	MYL1153		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCATGTGCTGATTGCTAGGACAGCTAGAGGTCG	TGTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.4484	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1154	100	9606				MYL1154	CRISPRko	CDS	MYL1154		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCGTGTGCTGATTGCTAGGACAGCTAGAGGTCG	TGTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.4510	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1155	100	9606				MYL1155	CRISPRko	CDS	MYL1155		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCTTGTGCTGATTGCTAGGACAGCTAGAGGTCG	TGTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.4481	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1155	100	9606				MYL1155	CRISPRko	CDS	MYL1155		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGACAGCTAGA	CCACCTTGTGCTGATTGCTAGGACAGCTAGAGGT	CTTG	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	1	1	0	0	12	4	13	9	1604	867	2746	enPAM+GB	0.4544	1	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGACAGCTAGA (for target MYL1139)
MYL1156	100	9606				MYL1156	CRISPRko	CDS	MYL1156		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCCTGTGCTGATTGCTAGGACAGCTAGAGGTCG	TGTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.3213	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1160	100	9606				MYL1160	CRISPRko	CDS	MYL1160		enAsCas12a	5	1	1	5.20.100		sense	30	GTGCTGATTGCTAGGACAGCTAG	ACCACCCCGTGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	1	0	0	0	14	3	3	10	1023	571	1640	enPAM+GB	0.5369	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL1161	100	9606				MYL1161	CRISPRko	CDS	MYL1161		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCAATTGCTGATTGCTAGGACAGCTAGAGGTCG	ATTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.5385	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1162	100	9606				MYL1162	CRISPRko	CDS	MYL1162		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCGATTGCTGATTGCTAGGACAGCTAGAGGTCG	ATTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.5411	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1163	100	9606				MYL1163	CRISPRko	CDS	MYL1163		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCTATTGCTGATTGCTAGGACAGCTAGAGGTCG	ATTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.5180	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1164	100	9606				MYL1164	CRISPRko	CDS	MYL1164		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCCATTGCTGATTGCTAGGACAGCTAGAGGTCG	ATTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.3445	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1165	100	9606				MYL1165	CRISPRko	CDS	MYL1165		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCAGTTGCTGATTGCTAGGACAGCTAGAGGTCG	GTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.5473	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1166	100	9606				MYL1166	CRISPRko	CDS	MYL1166		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCGGTTGCTGATTGCTAGGACAGCTAGAGGTCG	GTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.5499	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1167	100	9606				MYL1167	CRISPRko	CDS	MYL1167		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCTGTTGCTGATTGCTAGGACAGCTAGAGGTCG	GTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.5425	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1168	100	9606				MYL1168	CRISPRko	CDS	MYL1168		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCCGTTGCTGATTGCTAGGACAGCTAGAGGTCG	GTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.3853	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1169	100	9606				MYL1169	CRISPRko	CDS	MYL1169		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCATTTGCTGATTGCTAGGACAGCTAGAGGTCG	TTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.7834	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1170	100	9606				MYL1170	CRISPRko	CDS	MYL1170		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCGTTTGCTGATTGCTAGGACAGCTAGAGGTCG	TTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.7861	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1171	100	9606				MYL1171	CRISPRko	CDS	MYL1171		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCTTTTGCTGATTGCTAGGACAGCTAGAGGTCG	TTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.7832	1	2	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1171	100	9606				MYL1171	CRISPRko	CDS	MYL1171		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTTTTGCTGATTGCTAGGACAGCTAGAGGTC	TTTT	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.5973	2	1	1.0	1.0	2	1	4	Previously skipped due to: Outside Target Window: 0-80%; Outside Target Window: 5-65%
MYL1172	100	9606				MYL1172	CRISPRko	CDS	MYL1172		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCCTTTGCTGATTGCTAGGACAGCTAGAGGTCG	TTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.7174	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1173	100	9606				MYL1173	CRISPRko	CDS	MYL1173		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCACTTGCTGATTGCTAGGACAGCTAGAGGTCG	CTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.5385	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1174	100	9606				MYL1174	CRISPRko	CDS	MYL1174		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCGCTTGCTGATTGCTAGGACAGCTAGAGGTCG	CTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.5411	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1175	100	9606				MYL1175	CRISPRko	CDS	MYL1175		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCTCTTGCTGATTGCTAGGACAGCTAGAGGTCG	CTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.5337	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1176	100	9606				MYL1176	CRISPRko	CDS	MYL1176		enAsCas12a	5	1	1	5.20.100		sense	30	TTGCTGATTGCTAGGACAGCTAG	ACCACCCCTTGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	0	76	47	147	10	1619	851	2662	enPAM+GB	0.4485	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL1176	100	9606				MYL1176	CRISPRko	CDS	MYL1176		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCCCTTGCTGATTGCTAGGACAGCTAGAGGTCG	CTTG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.3763	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1186	100	9606				MYL1186	CRISPRko	CDS	MYL1186		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGACAGCTAGA	CCACCGTCTGCTGATTGCTAGGACAGCTAGAGGT	CGTC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	1	1	0	0	12	4	13	9	1604	867	2746	enPAM+GB	0.3557	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGACAGCTAGA (for target MYL1139)
MYL1187	100	9606				MYL1187	CRISPRko	CDS	MYL1187		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTTCTGCTGATTGCTAGGACAGCTAGAGGTC	TTCT	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4526	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1187	100	9606				MYL1187	CRISPRko	CDS	MYL1187		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGACAGCTAGA	CCACCTTCTGCTGATTGCTAGGACAGCTAGAGGT	CTTC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	1	1	0	0	12	4	13	9	1604	867	2746	enPAM+GB	0.5599	1	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGACAGCTAGA (for target MYL1139)
MYL1189	100	9606				MYL1189	CRISPRko	CDS	MYL1189		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGACAGCTAGA	CCACCACCTGCTGATTGCTAGGACAGCTAGAGGT	CACC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	1	1	0	0	12	4	13	9	1604	867	2746	enPAM+GB	0.3373	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGACAGCTAGA (for target MYL1139)
MYL1190	100	9606				MYL1190	CRISPRko	CDS	MYL1190		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGACAGCTAGA	CCACCGCCTGCTGATTGCTAGGACAGCTAGAGGT	CGCC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	1	1	0	0	12	4	13	9	1604	867	2746	enPAM+GB	0.3422	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGACAGCTAGA (for target MYL1139)
MYL1191	100	9606				MYL1191	CRISPRko	CDS	MYL1191		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGACAGCTAGA	CCACCTCCTGCTGATTGCTAGGACAGCTAGAGGT	CTCC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	1	1	0	0	12	4	13	9	1604	867	2746	enPAM+GB	0.4373	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGACAGCTAGA (for target MYL1139)
MYL1192	100	9606				MYL1192	CRISPRko	CDS	MYL1192		enAsCas12a	5	1	1	5.20.100		sense	30	CTGCTGATTGCTAGGACAGCTAG	ACCACCCCCTGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	1	1	0	0	12	3	13	11	1039	565	1633	enPAM+GB	0.4906	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL1192	100	9606				MYL1192	CRISPRko	CDS	MYL1192		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGACAGCTAGA	CCACCCCCTGCTGATTGCTAGGACAGCTAGAGGT	CCCC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	1	1	0	0	12	4	13	9	1604	867	2746	enPAM+GB	0.3462	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGACAGCTAGA (for target MYL1139)
MYL1203	100	9606				MYL1203	CRISPRko	CDS	MYL1203		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGACAGCTAGA	CCACCTTACGCTGATTGCTAGGACAGCTAGAGGT	CTTA	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	5	7	15	6	1606	921	2928	enPAM+GB	0.5902	1	1	1.0	1.0	1	1	4	Previously skipped due to: Outside Target Window: 0-80%; Outside Target Window: 5-65%
MYL1206	100	9606				MYL1206	CRISPRko	CDS	MYL1206		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGACAGCTAGA	CCACCGCACGCTGATTGCTAGGACAGCTAGAGGT	CGCA	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	5	7	15	6	1606	921	2928	enPAM+GB	0.2775	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGACAGCTAGA (for target MYL1203)
MYL1207	100	9606				MYL1207	CRISPRko	CDS	MYL1207		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGACAGCTAGA	CCACCTCACGCTGATTGCTAGGACAGCTAGAGGT	CTCA	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	5	7	15	6	1606	921	2928	enPAM+GB	0.3140	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGACAGCTAGA (for target MYL1203)
MYL1208	100	9606				MYL1208	CRISPRko	CDS	MYL1208		enAsCas12a	5	1	1	5.20.100		sense	30	ACGCTGATTGCTAGGACAGCTAG	ACCACCCCACGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	1	0	0	0	6	0	4	4	1240	691	2113	enPAM+GB	0.5179	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL1219	100	9606				MYL1219	CRISPRko	CDS	MYL1219		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGACAGCTAGA	CCACCTTGCGCTGATTGCTAGGACAGCTAGAGGT	CTTG	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	5	7	15	6	1606	921	2928	enPAM+GB	0.4336	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGACAGCTAGA (for target MYL1203)
MYL1224	100	9606				MYL1224	CRISPRko	CDS	MYL1224		enAsCas12a	5	1	1	5.20.100		sense	30	GCGCTGATTGCTAGGACAGCTAG	ACCACCCCGCGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	9	4	10	3	1217	677	2081	enPAM+GB	0.5054	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL1225	100	9606				MYL1225	CRISPRko	CDS	MYL1225		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCAATCGCTGATTGCTAGGACAGCTAGAGGTC	AATC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3313	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1227	100	9606				MYL1227	CRISPRko	CDS	MYL1227		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTATCGCTGATTGCTAGGACAGCTAGAGGTC	TATC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4823	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1229	100	9606				MYL1229	CRISPRko	CDS	MYL1229		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCAGTCGCTGATTGCTAGGACAGCTAGAGGTC	AGTC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3355	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1231	100	9606				MYL1231	CRISPRko	CDS	MYL1231		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTGTCGCTGATTGCTAGGACAGCTAGAGGTC	TGTC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4668	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1232	100	9606				MYL1232	CRISPRko	CDS	MYL1232		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCCGTCGCTGATTGCTAGGACAGCTAGAGGTC	CGTC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3613	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1233	100	9606				MYL1233	CRISPRko	CDS	MYL1233		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCATTCGCTGATTGCTAGGACAGCTAGAGGTCG	TTCG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.6015	1	2	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1233	100	9606				MYL1233	CRISPRko	CDS	MYL1233		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCATTCGCTGATTGCTAGGACAGCTAGAGGTC	ATTC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.5266	2	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1234	100	9606				MYL1234	CRISPRko	CDS	MYL1234		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCGTTCGCTGATTGCTAGGACAGCTAGAGGTCG	TTCG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.6042	1	2	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1234	100	9606				MYL1234	CRISPRko	CDS	MYL1234		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCGTTCGCTGATTGCTAGGACAGCTAGAGGTC	GTTC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.5532	2	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1235	100	9606				MYL1235	CRISPRko	CDS	MYL1235		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTTTCGCTGATTGCTAGGACAGCTAGAGGTC	TTTC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.8157	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1235	100	9606				MYL1235	CRISPRko	CDS	MYL1235		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCTTTCGCTGATTGCTAGGACAGCTAGAGGTCG	TTCG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.6012	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1236	100	9606				MYL1236	CRISPRko	CDS	MYL1236		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCCTTCGCTGATTGCTAGGACAGCTAGAGGTC	CTTC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.5572	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1236	100	9606				MYL1236	CRISPRko	CDS	MYL1236		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGACAGCTAGAGG	ACCCTTCGCTGATTGCTAGGACAGCTAGAGGTCG	TTCG	1	32	37	86.5	NO_SITE (4 zeros)	0	0	0	0	0	1	0	0	1	92	63	186	3	887	492	1519	enPAM+GB	0.5164	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGACAGCTAGAGG (for target MYL1137)
MYL1240	100	9606				MYL1240	CRISPRko	CDS	MYL1240		enAsCas12a	5	1	1	5.20.100		sense	30	TCGCTGATTGCTAGGACAGCTAG	ACCACCCCTCGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	1	0	0	1	579	357	995	2	1045	582	2009	enPAM+GB	0.5054	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL1241	100	9606				MYL1241	CRISPRko	CDS	MYL1241		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCAACCGCTGATTGCTAGGACAGCTAGAGGTC	AACC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3339	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1243	100	9606				MYL1243	CRISPRko	CDS	MYL1243		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTACCGCTGATTGCTAGGACAGCTAGAGGTC	TACC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4875	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1244	100	9606				MYL1244	CRISPRko	CDS	MYL1244		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCCACCGCTGATTGCTAGGACAGCTAGAGGTC	CACC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3819	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1245	100	9606				MYL1245	CRISPRko	CDS	MYL1245		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCAGCCGCTGATTGCTAGGACAGCTAGAGGTC	AGCC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3388	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1247	100	9606				MYL1247	CRISPRko	CDS	MYL1247		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTGCCGCTGATTGCTAGGACAGCTAGAGGTC	TGCC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4598	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1248	100	9606				MYL1248	CRISPRko	CDS	MYL1248		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCCGCCGCTGATTGCTAGGACAGCTAGAGGTC	CGCC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3772	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1249	100	9606				MYL1249	CRISPRko	CDS	MYL1249		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCATCCGCTGATTGCTAGGACAGCTAGAGGTC	ATCC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4308	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1250	100	9606				MYL1250	CRISPRko	CDS	MYL1250		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCGTCCGCTGATTGCTAGGACAGCTAGAGGTC	GTCC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4555	1	2	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1250	100	9606				MYL1250	CRISPRko	CDS	MYL1250		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGACAGCTAGA	CCACCGTCCGCTGATTGCTAGGACAGCTAGAGGT	CGTC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	5	7	15	6	1606	921	2928	enPAM+GB	0.3493	2	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGACAGCTAGA (for target MYL1203)
MYL1251	100	9606				MYL1251	CRISPRko	CDS	MYL1251		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTTCCGCTGATTGCTAGGACAGCTAGAGGTC	TTCC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.7106	1	2	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1251	100	9606				MYL1251	CRISPRko	CDS	MYL1251		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGACAGCTAGA	CCACCTTCCGCTGATTGCTAGGACAGCTAGAGGT	CTTC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	5	7	15	6	1606	921	2928	enPAM+GB	0.5534	2	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGACAGCTAGA (for target MYL1203)
MYL1252	100	9606				MYL1252	CRISPRko	CDS	MYL1252		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCCTCCGCTGATTGCTAGGACAGCTAGAGGTC	CTCC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4788	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1253	100	9606				MYL1253	CRISPRko	CDS	MYL1253		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCACCCGCTGATTGCTAGGACAGCTAGAGGTC	ACCC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3261	1	2	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1253	100	9606				MYL1253	CRISPRko	CDS	MYL1253		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGACAGCTAGA	CCACCACCCGCTGATTGCTAGGACAGCTAGAGGT	CACC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	5	7	15	6	1606	921	2928	enPAM+GB	0.3224	2	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGACAGCTAGA (for target MYL1203)
MYL1254	100	9606				MYL1254	CRISPRko	CDS	MYL1254		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGACAGCTAGA	CCACCGCCCGCTGATTGCTAGGACAGCTAGAGGT	CGCC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	5	7	15	6	1606	921	2928	enPAM+GB	0.3274	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGACAGCTAGA (for target MYL1203)
MYL1255	100	9606				MYL1255	CRISPRko	CDS	MYL1255		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTCCCGCTGATTGCTAGGACAGCTAGAGGTC	TCCC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4924	1	2	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
MYL1255	100	9606				MYL1255	CRISPRko	CDS	MYL1255		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGACAGCTAGA	CCACCTCCCGCTGATTGCTAGGACAGCTAGAGGT	CTCC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	5	7	15	6	1606	921	2928	enPAM+GB	0.4225	2	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGACAGCTAGA (for target MYL1203)
MYL1256	100	9606				MYL1256	CRISPRko	CDS	MYL1256		enAsCas12a	5	1	1	5.20.100		sense	30	CCGCTGATTGCTAGGACAGCTAG	ACCACCCCCCGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	11	2	17	7	1225	686	2091	enPAM+GB	0.4956	1	2	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL1256	100	9606				MYL1256	CRISPRko	CDS	MYL1256		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGACAGCTAGA	CCACCCCCCGCTGATTGCTAGGACAGCTAGAGGT	CCCC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	5	7	15	6	1606	921	2928	enPAM+GB	0.3314	3	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGACAGCTAGA (for target MYL1203)
MYL1256	100	9606				MYL1256	CRISPRko	CDS	MYL1256		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCCCCCGCTGATTGCTAGGACAGCTAGAGGTC	CCCC	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3908	2	3	1.0	1.0	3			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL1171)
