Input	Quota	Target Taxon	Target Gene ID	Target Gene Symbol	Target Transcript	Target Alias	CRISPR Mechanism	Target Domain	Reference Sequence	Strand of Target	PAM Policy	Initial Spacing Requirement	Off-Target Match Ruleset Version	Off-Target Tier Policy	Off-Target Match Bin Policy	Strand of sgRNA	Orientation	sgRNA Cut Position (1-based)	sgRNA Sequence	sgRNA Context Sequence	PAM Sequence	Exon Number	Target Cut Length	Target Total Length	Target Cut %	Other Target Matches	# Off-Target Tier I Match Bin I Matches	# Off-Target Tier II Match Bin I Matches	# Off-Target Tier III Match Bin I Matches	# Off-Target Tier IV Match Bin I Matches	# Off-Target Tier I Match Bin II Matches	# Off-Target Tier II Match Bin II Matches	# Off-Target Tier III Match Bin II Matches	# Off-Target Tier IV Match Bin II Matches	# Off-Target Tier I Match Bin III Matches	# Off-Target Tier II Match Bin III Matches	# Off-Target Tier III Match Bin III Matches	# Off-Target Tier IV Match Bin III Matches	# Off-Target Tier I Match Bin IV Matches	# Off-Target Tier II Match Bin IV Matches	# Off-Target Tier III Match Bin IV Matches	# Off-Target Tier IV Match Bin IV Matches	On-Target Ruleset	On-Target Efficacy Score	On-Target Rank	Off-Target Rank	On-Target Rank Weight	Off-Target Rank Weight	Combined Rank	Pick Order	Picking Round	Picking Notes
MYL111	100	9606				MYL111	CRISPRko	CDS	MYL111		enAsCas12a	5	1	1	5.20.100		sense	31	AGCTGATTGCTAGGACAGCTAGA	CCACCTTAAGCTGATTGCTAGGACAGCTAGAGGT	CTTA	1	30	37	81.1	NO_SITE (6 zeros)	0	0	0	0	0	0	0	0	0	7	4	7	10	1457	778	2410	enPAM+GB	0.6190	1	1	1.0	1.0	1	1	4	Previously skipped due to: Outside Target Window: 0-80%; Outside Target Window: 5-65%
MYL114	100	9606				MYL114	CRISPRko	CDS	MYL114		enAsCas12a	5	1	1	5.20.100		sense	31	AGCTGATTGCTAGGACAGCTAGA	CCACCGCAAGCTGATTGCTAGGACAGCTAGAGGT	CGCA	1	30	37	81.1	NO_SITE (6 zeros)	0	0	0	0	0	0	0	0	0	7	4	7	10	1457	778	2410	enPAM+GB	0.2940	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with AGCTGATTGCTAGGACAGCTAGA (for target MYL111)
MYL115	100	9606				MYL115	CRISPRko	CDS	MYL115		enAsCas12a	5	1	1	5.20.100		sense	31	AGCTGATTGCTAGGACAGCTAGA	CCACCTCAAGCTGATTGCTAGGACAGCTAGAGGT	CTCA	1	30	37	81.1	NO_SITE (6 zeros)	0	0	0	0	0	0	0	0	0	7	4	7	10	1457	778	2410	enPAM+GB	0.3512	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with AGCTGATTGCTAGGACAGCTAGA (for target MYL111)
MYL116	100	9606				MYL116	CRISPRko	CDS	MYL116		enAsCas12a	5	1	1	5.20.100		sense	30	AAGCTGATTGCTAGGACAGCTAG	ACCACCCCAAGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	1	0	0	0	9	3	5	11	906	510	1376	enPAM+GB	0.5258	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL127	100	9606				MYL127	CRISPRko	CDS	MYL127		enAsCas12a	5	1	1	5.20.100		sense	31	AGCTGATTGCTAGGACAGCTAGA	CCACCTTGAGCTGATTGCTAGGACAGCTAGAGGT	CTTG	1	30	37	81.1	NO_SITE (6 zeros)	0	0	0	0	0	0	0	0	0	7	4	7	10	1457	778	2410	enPAM+GB	0.4624	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with AGCTGATTGCTAGGACAGCTAGA (for target MYL111)
MYL132	100	9606				MYL132	CRISPRko	CDS	MYL132		enAsCas12a	5	1	1	5.20.100		sense	30	GAGCTGATTGCTAGGACAGCTAG	ACCACCCCGAGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	0	10	5	4	6	888	482	1327	enPAM+GB	0.5054	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL133	100	9606				MYL133	CRISPRko	CDS	MYL133		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCAATAGCTGATTGCTAGGACAGCTAGAGGTC	AATA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3300	1	1	1.0	1.0	1	1	4	Previously skipped due to: Outside Target Window: 0-80%; Outside Target Window: 5-65%
MYL135	100	9606				MYL135	CRISPRko	CDS	MYL135		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTATAGCTGATTGCTAGGACAGCTAGAGGTC	TATA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4667	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL137	100	9606				MYL137	CRISPRko	CDS	MYL137		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCAGTAGCTGATTGCTAGGACAGCTAGAGGTC	AGTA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3389	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL139	100	9606				MYL139	CRISPRko	CDS	MYL139		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTGTAGCTGATTGCTAGGACAGCTAGAGGTC	TGTA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4573	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL141	100	9606				MYL141	CRISPRko	CDS	MYL141		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCATTAGCTGATTGCTAGGACAGCTAGAGGTC	ATTA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.5461	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL142	100	9606				MYL142	CRISPRko	CDS	MYL142		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCGTTAGCTGATTGCTAGGACAGCTAGAGGTC	GTTA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.5975	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL143	100	9606				MYL143	CRISPRko	CDS	MYL143		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTTTAGCTGATTGCTAGGACAGCTAGAGGTC	TTTA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.8333	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL144	100	9606				MYL144	CRISPRko	CDS	MYL144		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCCTTAGCTGATTGCTAGGACAGCTAGAGGTC	CTTA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.5934	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL148	100	9606				MYL148	CRISPRko	CDS	MYL148		enAsCas12a	5	1	1	5.20.100		sense	30	TAGCTGATTGCTAGGACAGCTAG	ACCACCCCTAGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	1	0	0	1	11	3	16	3	1434	780	2409	enPAM+GB	0.5114	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL151	100	9606				MYL151	CRISPRko	CDS	MYL151		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTACAGCTGATTGCTAGGACAGCTAGAGGTC	TACA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3998	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL155	100	9606				MYL155	CRISPRko	CDS	MYL155		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTGCAGCTGATTGCTAGGACAGCTAGAGGTC	TGCA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3726	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL156	100	9606				MYL156	CRISPRko	CDS	MYL156		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCCGCAGCTGATTGCTAGGACAGCTAGAGGTC	CGCA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3150	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL157	100	9606				MYL157	CRISPRko	CDS	MYL157		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCATCAGCTGATTGCTAGGACAGCTAGAGGTC	ATCA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3518	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL158	100	9606				MYL158	CRISPRko	CDS	MYL158		enAsCas12a	5	1	1	5.20.100		sense	31	AGCTGATTGCTAGGACAGCTAGA	CCACCGTCAGCTGATTGCTAGGACAGCTAGAGGT	CGTC	1	30	37	81.1	NO_SITE (6 zeros)	0	0	0	0	0	0	0	0	0	7	4	7	10	1457	778	2410	enPAM+GB	0.3911	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with AGCTGATTGCTAGGACAGCTAGA (for target MYL111)
MYL158	100	9606				MYL158	CRISPRko	CDS	MYL158		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCGTCAGCTGATTGCTAGGACAGCTAGAGGTC	GTCA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3636	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL159	100	9606				MYL159	CRISPRko	CDS	MYL159		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTTCAGCTGATTGCTAGGACAGCTAGAGGTC	TTCA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.6316	1	2	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL159	100	9606				MYL159	CRISPRko	CDS	MYL159		enAsCas12a	5	1	1	5.20.100		sense	31	AGCTGATTGCTAGGACAGCTAGA	CCACCTTCAGCTGATTGCTAGGACAGCTAGAGGT	CTTC	1	30	37	81.1	NO_SITE (6 zeros)	0	0	0	0	0	0	0	0	0	7	4	7	10	1457	778	2410	enPAM+GB	0.5953	2	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with AGCTGATTGCTAGGACAGCTAGA (for target MYL111)
MYL160	100	9606				MYL160	CRISPRko	CDS	MYL160		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCCTCAGCTGATTGCTAGGACAGCTAGAGGTC	CTCA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3836	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL161	100	9606				MYL161	CRISPRko	CDS	MYL161		enAsCas12a	5	1	1	5.20.100		sense	31	AGCTGATTGCTAGGACAGCTAGA	CCACCACCAGCTGATTGCTAGGACAGCTAGAGGT	CACC	1	30	37	81.1	NO_SITE (6 zeros)	0	0	0	0	0	0	0	0	0	7	4	7	10	1457	778	2410	enPAM+GB	0.3727	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with AGCTGATTGCTAGGACAGCTAGA (for target MYL111)
MYL162	100	9606				MYL162	CRISPRko	CDS	MYL162		enAsCas12a	5	1	1	5.20.100		sense	31	AGCTGATTGCTAGGACAGCTAGA	CCACCGCCAGCTGATTGCTAGGACAGCTAGAGGT	CGCC	1	30	37	81.1	NO_SITE (6 zeros)	0	0	0	0	0	0	0	0	0	7	4	7	10	1457	778	2410	enPAM+GB	0.3776	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with AGCTGATTGCTAGGACAGCTAGA (for target MYL111)
MYL163	100	9606				MYL163	CRISPRko	CDS	MYL163		enAsCas12a	5	1	1	5.20.100		sense	31	AGCTGATTGCTAGGACAGCTAGA	CCACCTCCAGCTGATTGCTAGGACAGCTAGAGGT	CTCC	1	30	37	81.1	NO_SITE (6 zeros)	0	0	0	0	0	0	0	0	0	7	4	7	10	1457	778	2410	enPAM+GB	0.4727	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with AGCTGATTGCTAGGACAGCTAGA (for target MYL111)
MYL163	100	9606				MYL163	CRISPRko	CDS	MYL163		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTCCAGCTGATTGCTAGGACAGCTAGAGGTC	TCCA	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3814	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL164	100	9606				MYL164	CRISPRko	CDS	MYL164		enAsCas12a	5	1	1	5.20.100		sense	31	AGCTGATTGCTAGGACAGCTAGA	CCACCCCCAGCTGATTGCTAGGACAGCTAGAGGT	CCCC	1	30	37	81.1	NO_SITE (6 zeros)	0	0	0	0	0	0	0	0	0	7	4	7	10	1457	778	2410	enPAM+GB	0.3816	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with AGCTGATTGCTAGGACAGCTAGA (for target MYL111)
MYL164	100	9606				MYL164	CRISPRko	CDS	MYL164		enAsCas12a	5	1	1	5.20.100		sense	30	CAGCTGATTGCTAGGACAGCTAG	ACCACCCCCAGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	11	1	7	5	907	515	1376	enPAM+GB	0.4906	1	2	1.0	1.0	2	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL175	100	9606				MYL175	CRISPRko	CDS	MYL175		enAsCas12a	5	1	1	5.20.100		sense	31	GGCTGATTGCTAGGACAGCTAGA	CCACCTTAGGCTGATTGCTAGGACAGCTAGAGGT	CTTA	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	0	0	1	0	8	2	10	6	1408	808	2414	enPAM+GB	0.6324	1	1	1.0	1.0	1	1	4	Previously skipped due to: Outside Target Window: 0-80%; Outside Target Window: 5-65%
MYL178	100	9606				MYL178	CRISPRko	CDS	MYL178		enAsCas12a	5	1	1	5.20.100		sense	31	GGCTGATTGCTAGGACAGCTAGA	CCACCGCAGGCTGATTGCTAGGACAGCTAGAGGT	CGCA	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	0	0	1	0	8	2	10	6	1408	808	2414	enPAM+GB	0.3133	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GGCTGATTGCTAGGACAGCTAGA (for target MYL175)
MYL179	100	9606				MYL179	CRISPRko	CDS	MYL179		enAsCas12a	5	1	1	5.20.100		sense	31	GGCTGATTGCTAGGACAGCTAGA	CCACCTCAGGCTGATTGCTAGGACAGCTAGAGGT	CTCA	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	0	0	1	0	8	2	10	6	1408	808	2414	enPAM+GB	0.3706	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GGCTGATTGCTAGGACAGCTAGA (for target MYL175)
MYL180	100	9606				MYL180	CRISPRko	CDS	MYL180		enAsCas12a	5	1	1	5.20.100		sense	30	AGGCTGATTGCTAGGACAGCTAG	ACCACCCCAGGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	1	0	1	1	9	2	7	4	925	543	1400	enPAM+GB	0.5179	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL191	100	9606				MYL191	CRISPRko	CDS	MYL191		enAsCas12a	5	1	1	5.20.100		sense	31	GGCTGATTGCTAGGACAGCTAGA	CCACCTTGGGCTGATTGCTAGGACAGCTAGAGGT	CTTG	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	0	0	1	0	8	2	10	6	1408	808	2414	enPAM+GB	0.4707	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GGCTGATTGCTAGGACAGCTAGA (for target MYL175)
MYL196	100	9606				MYL196	CRISPRko	CDS	MYL196		enAsCas12a	5	1	1	5.20.100		sense	30	GGGCTGATTGCTAGGACAGCTAG	ACCACCCCGGGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	7	2	7	6	889	519	1367	enPAM+GB	0.4906	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL199	100	9606				MYL199	CRISPRko	CDS	MYL199		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTATGGCTGATTGCTAGGACAGCTAGAGGTC	TATG	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4202	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL1103	100	9606				MYL1103	CRISPRko	CDS	MYL1103		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTGTGGCTGATTGCTAGGACAGCTAGAGGTC	TGTG	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.3970	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL1105	100	9606				MYL1105	CRISPRko	CDS	MYL1105		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCATTGGCTGATTGCTAGGACAGCTAGAGGTC	ATTG	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4327	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL1106	100	9606				MYL1106	CRISPRko	CDS	MYL1106		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCGTTGGCTGATTGCTAGGACAGCTAGAGGTC	GTTG	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4638	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL1107	100	9606				MYL1107	CRISPRko	CDS	MYL1107		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTTTGGCTGATTGCTAGGACAGCTAGAGGTC	TTTG	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.7922	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL1108	100	9606				MYL1108	CRISPRko	CDS	MYL1108		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCCTTGGCTGATTGCTAGGACAGCTAGAGGTC	CTTG	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.4597	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL1112	100	9606				MYL1112	CRISPRko	CDS	MYL1112		enAsCas12a	5	1	1	5.20.100		sense	30	TGGCTGATTGCTAGGACAGCTAG	ACCACCCCTGGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	1	0	0	0	26	15	55	13	1475	817	2484	enPAM+GB	0.5054	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL1122	100	9606				MYL1122	CRISPRko	CDS	MYL1122		enAsCas12a	5	1	1	5.20.100		sense	31	GGCTGATTGCTAGGACAGCTAGA	CCACCGTCGGCTGATTGCTAGGACAGCTAGAGGT	CGTC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	0	0	1	0	8	2	10	6	1408	808	2414	enPAM+GB	0.3641	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GGCTGATTGCTAGGACAGCTAGA (for target MYL175)
MYL1123	100	9606				MYL1123	CRISPRko	CDS	MYL1123		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGACAGCTAGAG	CACCTTCGGCTGATTGCTAGGACAGCTAGAGGTC	TTCG	1	31	37	83.8	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	1	15	8	21	5	1423	763	2415	enPAM+GB	0.5863	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGACAGCTAGAG (for target MYL133)
MYL1123	100	9606				MYL1123	CRISPRko	CDS	MYL1123		enAsCas12a	5	1	1	5.20.100		sense	31	GGCTGATTGCTAGGACAGCTAGA	CCACCTTCGGCTGATTGCTAGGACAGCTAGAGGT	CTTC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	0	0	1	0	8	2	10	6	1408	808	2414	enPAM+GB	0.5683	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GGCTGATTGCTAGGACAGCTAGA (for target MYL175)
MYL1125	100	9606				MYL1125	CRISPRko	CDS	MYL1125		enAsCas12a	5	1	1	5.20.100		sense	31	GGCTGATTGCTAGGACAGCTAGA	CCACCACCGGCTGATTGCTAGGACAGCTAGAGGT	CACC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	0	0	1	0	8	2	10	6	1408	808	2414	enPAM+GB	0.3373	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GGCTGATTGCTAGGACAGCTAGA (for target MYL175)
MYL1126	100	9606				MYL1126	CRISPRko	CDS	MYL1126		enAsCas12a	5	1	1	5.20.100		sense	31	GGCTGATTGCTAGGACAGCTAGA	CCACCGCCGGCTGATTGCTAGGACAGCTAGAGGT	CGCC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	0	0	1	0	8	2	10	6	1408	808	2414	enPAM+GB	0.3422	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GGCTGATTGCTAGGACAGCTAGA (for target MYL175)
MYL1127	100	9606				MYL1127	CRISPRko	CDS	MYL1127		enAsCas12a	5	1	1	5.20.100		sense	31	GGCTGATTGCTAGGACAGCTAGA	CCACCTCCGGCTGATTGCTAGGACAGCTAGAGGT	CTCC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	0	0	1	0	8	2	10	6	1408	808	2414	enPAM+GB	0.4373	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GGCTGATTGCTAGGACAGCTAGA (for target MYL175)
MYL1128	100	9606				MYL1128	CRISPRko	CDS	MYL1128		enAsCas12a	5	1	1	5.20.100		sense	30	CGGCTGATTGCTAGGACAGCTAG	ACCACCCCCGGCTGATTGCTAGGACAGCTAGAGG	CCCC	1	29	37	78.4	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	3	0	2	4	747	466	1262	enPAM+GB	0.4819	1	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYL1128	100	9606				MYL1128	CRISPRko	CDS	MYL1128		enAsCas12a	5	1	1	5.20.100		sense	31	GGCTGATTGCTAGGACAGCTAGA	CCACCCCCGGCTGATTGCTAGGACAGCTAGAGGT	CCCC	1	30	37	81.1	NO_SITE (5 zeros)	0	0	0	0	0	0	0	1	0	8	2	10	6	1408	808	2414	enPAM+GB	0.3462	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GGCTGATTGCTAGGACAGCTAGA (for target MYL175)
