Input	Quota	Target Taxon	Target Gene ID	Target Gene Symbol	Target Transcript	Target Alias	CRISPR Mechanism	Target Domain	Reference Sequence	Strand of Target	PAM Policy	Initial Spacing Requirement	Off-Target Match Ruleset Version	Off-Target Tier Policy	Off-Target Match Bin Policy	Strand of sgRNA	Orientation	sgRNA Cut Position (1-based)	sgRNA Sequence	sgRNA Context Sequence	PAM Sequence	Exon Number	Target Cut Length	Target Total Length	Target Cut %	Other Target Matches	# Off-Target Tier I Match Bin I Matches	# Off-Target Tier II Match Bin I Matches	# Off-Target Tier III Match Bin I Matches	# Off-Target Tier IV Match Bin I Matches	# Off-Target Tier I Match Bin II Matches	# Off-Target Tier II Match Bin II Matches	# Off-Target Tier III Match Bin II Matches	# Off-Target Tier IV Match Bin II Matches	# Off-Target Tier I Match Bin III Matches	# Off-Target Tier II Match Bin III Matches	# Off-Target Tier III Match Bin III Matches	# Off-Target Tier IV Match Bin III Matches	# Off-Target Tier I Match Bin IV Matches	# Off-Target Tier II Match Bin IV Matches	# Off-Target Tier III Match Bin IV Matches	# Off-Target Tier IV Match Bin IV Matches	On-Target Ruleset	On-Target Efficacy Score	On-Target Rank	Off-Target Rank	On-Target Rank Weight	Off-Target Rank Weight	Combined Rank	Pick Order	Picking Round	Picking Notes
MYC1_129	100	9606				MYC1_129	CRISPRko	CDS	MYC1_129		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATTTG	GGCTCTTAGCCCTCCTAGCAATCAGCATTTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	2	6	265	112	296	enPAM+GB	0.7449	1	1	1.0	1.0	1	1	1	
MYC1_130	100	9606				MYC1_130	CRISPRko	CDS	MYC1_130		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATTCG	GGCTCTTAGCCCTCCTAGCAATCAGCATTCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	2	2	6	264	112	296	enPAM+GB	0.7352	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_131	100	9606				MYC1_131	CRISPRko	CDS	MYC1_131		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATTAG	GGCTCTTAGCCCTCCTAGCAATCAGCATTAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7567	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_132	100	9606				MYC1_132	CRISPRko	CDS	MYC1_132		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATTGG	GGCTCTTAGCCCTCCTAGCAATCAGCATTGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7039	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_133	100	9606				MYC1_133	CRISPRko	CDS	MYC1_133		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATCTG	GGCTCTTAGCCCTCCTAGCAATCAGCATCTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	1	3	6	265	113	296	enPAM+GB	0.7918	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_134	100	9606				MYC1_134	CRISPRko	CDS	MYC1_134		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATCCG	GGCTCTTAGCCCTCCTAGCAATCAGCATCCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	1	2	6	265	113	296	enPAM+GB	0.7977	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_135	100	9606				MYC1_135	CRISPRko	CDS	MYC1_135		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATCAG	GGCTCTTAGCCCTCCTAGCAATCAGCATCAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	1	1	6	265	113	296	enPAM+GB	0.7991	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_136	100	9606				MYC1_136	CRISPRko	CDS	MYC1_136		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATCGG	GGCTCTTAGCCCTCCTAGCAATCAGCATCGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7678	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_137	100	9606				MYC1_137	CRISPRko	CDS	MYC1_137		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATATG	GGCTCTTAGCCCTCCTAGCAATCAGCATATGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	1	2	6	265	113	295	enPAM+GB	0.7865	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_137	100	9606				MYC1_137	CRISPRko	CDS	MYC1_137		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCATATGCTGATTGCTAGGAGGGCTAAGAGCCG	TATG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.4815	2	2	1.0	1.0	2	1	4	Previously skipped due to: Outside Target Window: 0-80%; Outside Target Window: 5-65%
MYC1_138	100	9606				MYC1_138	CRISPRko	CDS	MYC1_138		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATACG	GGCTCTTAGCCCTCCTAGCAATCAGCATACGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	1	2	6	265	113	295	enPAM+GB	0.7972	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_138	100	9606				MYC1_138	CRISPRko	CDS	MYC1_138		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCGTATGCTGATTGCTAGGAGGGCTAAGAGCCG	TATG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.4794	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_139	100	9606				MYC1_139	CRISPRko	CDS	MYC1_139		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGAGGGCTAAG	TGACCTTATGCTGATTGCTAGGAGGGCTAAGAGC	CTTA	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	8	2	11	6	259	113	286	enPAM+GB	0.5403	2	1	1.0	1.0	1	1	4	Previously skipped due to: Outside Target Window: 0-80%; Outside Target Window: 5-65%
MYC1_139	100	9606				MYC1_139	CRISPRko	CDS	MYC1_139		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATAAG	GGCTCTTAGCCCTCCTAGCAATCAGCATAAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	1	2	6	264	113	295	enPAM+GB	0.7944	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_139	100	9606				MYC1_139	CRISPRko	CDS	MYC1_139		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCTTATGCTGATTGCTAGGAGGGCTAAGAGCCG	TATG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.4812	3	3	1.0	1.0	3			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_140	100	9606				MYC1_140	CRISPRko	CDS	MYC1_140		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATAGG	GGCTCTTAGCCCTCCTAGCAATCAGCATAGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	2	6	265	112	295	enPAM+GB	0.7565	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_140	100	9606				MYC1_140	CRISPRko	CDS	MYC1_140		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCCTATGCTGATTGCTAGGAGGGCTAAGAGCCG	TATG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.3396	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_141	100	9606				MYC1_141	CRISPRko	CDS	MYC1_141		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATGTG	GGCTCTTAGCCCTCCTAGCAATCAGCATGTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	1	3	6	265	113	295	enPAM+GB	0.7571	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_142	100	9606				MYC1_142	CRISPRko	CDS	MYC1_142		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGAGGGCTAAG	TGACCGCATGCTGATTGCTAGGAGGGCTAAGAGC	CGCA	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	8	2	11	6	259	113	286	enPAM+GB	0.2273	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGAGGGCTAAG (for target MYC1_139)
MYC1_142	100	9606				MYC1_142	CRISPRko	CDS	MYC1_142		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATGCG	GGCTCTTAGCCCTCCTAGCAATCAGCATGCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	1	2	6	265	113	296	enPAM+GB	0.7748	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_143	100	9606				MYC1_143	CRISPRko	CDS	MYC1_143		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGAGGGCTAAG	TGACCTCATGCTGATTGCTAGGAGGGCTAAGAGC	CTCA	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	8	2	11	6	259	113	286	enPAM+GB	0.2845	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGAGGGCTAAG (for target MYC1_139)
MYC1_143	100	9606				MYC1_143	CRISPRko	CDS	MYC1_143		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATGAG	GGCTCTTAGCCCTCCTAGCAATCAGCATGAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7655	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_144	100	9606				MYC1_144	CRISPRko	CDS	MYC1_144		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCATGGG	GGCTCTTAGCCCTCCTAGCAATCAGCATGGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.6966	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_144	100	9606				MYC1_144	CRISPRko	CDS	MYC1_144		enAsCas12a	5	1	1	5.20.100		sense	30	ATGCTGATTGCTAGGAGGGCTAA	ATGACCCCATGCTGATTGCTAGGAGGGCTAAGAG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	0	1	2	1	10	7	14	10	220	78	204	enPAM+GB	0.5207	2	2	1.0	1.0	2	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYC1_145	100	9606				MYC1_145	CRISPRko	CDS	MYC1_145		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACTTG	GGCTCTTAGCCCTCCTAGCAATCAGCACTTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	0	2	2	6	266	112	296	enPAM+GB	0.7497	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_146	100	9606				MYC1_146	CRISPRko	CDS	MYC1_146		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACTCG	GGCTCTTAGCCCTCCTAGCAATCAGCACTCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	3	2	2	6	263	112	296	enPAM+GB	0.7802	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_147	100	9606				MYC1_147	CRISPRko	CDS	MYC1_147		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACTAG	GGCTCTTAGCCCTCCTAGCAATCAGCACTAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	2	6	265	112	296	enPAM+GB	0.7972	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_148	100	9606				MYC1_148	CRISPRko	CDS	MYC1_148		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACTGG	GGCTCTTAGCCCTCCTAGCAATCAGCACTGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7818	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_149	100	9606				MYC1_149	CRISPRko	CDS	MYC1_149		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACCTG	GGCTCTTAGCCCTCCTAGCAATCAGCACCTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	0	1	3	6	266	113	296	enPAM+GB	0.7896	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_150	100	9606				MYC1_150	CRISPRko	CDS	MYC1_150		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACCCG	GGCTCTTAGCCCTCCTAGCAATCAGCACCCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	1	2	6	265	113	296	enPAM+GB	0.7896	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_151	100	9606				MYC1_151	CRISPRko	CDS	MYC1_151		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACCAG	GGCTCTTAGCCCTCCTAGCAATCAGCACCAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	2	6	265	112	296	enPAM+GB	0.7969	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_152	100	9606				MYC1_152	CRISPRko	CDS	MYC1_152		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACCGG	GGCTCTTAGCCCTCCTAGCAATCAGCACCGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7818	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_153	100	9606				MYC1_153	CRISPRko	CDS	MYC1_153		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACATG	GGCTCTTAGCCCTCCTAGCAATCAGCACATGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	1	3	6	265	113	295	enPAM+GB	0.7910	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_153	100	9606				MYC1_153	CRISPRko	CDS	MYC1_153		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCATGTGCTGATTGCTAGGAGGGCTAAGAGCCG	TGTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.4441	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_154	100	9606				MYC1_154	CRISPRko	CDS	MYC1_154		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACACG	GGCTCTTAGCCCTCCTAGCAATCAGCACACGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	3	6	265	112	295	enPAM+GB	0.7969	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_154	100	9606				MYC1_154	CRISPRko	CDS	MYC1_154		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCGTGTGCTGATTGCTAGGAGGGCTAAGAGCCG	TGTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.4468	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_155	100	9606				MYC1_155	CRISPRko	CDS	MYC1_155		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACAAG	GGCTCTTAGCCCTCCTAGCAATCAGCACAAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	2	2	6	264	112	295	enPAM+GB	0.7910	1	2	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_155	100	9606				MYC1_155	CRISPRko	CDS	MYC1_155		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGAGGGCTAAG	TGACCTTGTGCTGATTGCTAGGAGGGCTAAGAGC	CTTG	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	8	2	11	6	259	113	286	enPAM+GB	0.4381	3	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGAGGGCTAAG (for target MYC1_139)
MYC1_155	100	9606				MYC1_155	CRISPRko	CDS	MYC1_155		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCTTGTGCTGATTGCTAGGAGGGCTAAGAGCCG	TGTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.4438	2	3	1.0	1.0	3			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_156	100	9606				MYC1_156	CRISPRko	CDS	MYC1_156		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACAGG	GGCTCTTAGCCCTCCTAGCAATCAGCACAGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	2	6	265	112	295	enPAM+GB	0.7891	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_156	100	9606				MYC1_156	CRISPRko	CDS	MYC1_156		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCCTGTGCTGATTGCTAGGAGGGCTAAGAGCCG	TGTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.3211	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_157	100	9606				MYC1_157	CRISPRko	CDS	MYC1_157		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACGTG	GGCTCTTAGCCCTCCTAGCAATCAGCACGTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	3	6	265	112	295	enPAM+GB	0.7777	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_158	100	9606				MYC1_158	CRISPRko	CDS	MYC1_158		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACGCG	GGCTCTTAGCCCTCCTAGCAATCAGCACGCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	2	6	265	112	296	enPAM+GB	0.7748	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_159	100	9606				MYC1_159	CRISPRko	CDS	MYC1_159		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACGAG	GGCTCTTAGCCCTCCTAGCAATCAGCACGAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7821	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_160	100	9606				MYC1_160	CRISPRko	CDS	MYC1_160		enAsCas12a	5	1	1	5.20.100		sense	30	GTGCTGATTGCTAGGAGGGCTAA	ATGACCCCGTGCTGATTGCTAGGAGGGCTAAGAG	CCCC	1	29	37	78.4	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	11	2	6	11	254	95	217	enPAM+GB	0.5183	2	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYC1_160	100	9606				MYC1_160	CRISPRko	CDS	MYC1_160		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCACGGG	GGCTCTTAGCCCTCCTAGCAATCAGCACGGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7505	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_161	100	9606				MYC1_161	CRISPRko	CDS	MYC1_161		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAATTG	GGCTCTTAGCCCTCCTAGCAATCAGCAATTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	0	2	3	6	266	112	295	enPAM+GB	0.7565	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_161	100	9606				MYC1_161	CRISPRko	CDS	MYC1_161		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCAATTGCTGATTGCTAGGAGGGCTAAGAGCCG	ATTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5342	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_162	100	9606				MYC1_162	CRISPRko	CDS	MYC1_162		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAATCG	GGCTCTTAGCCCTCCTAGCAATCAGCAATCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	2	6	265	112	296	enPAM+GB	0.7859	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_162	100	9606				MYC1_162	CRISPRko	CDS	MYC1_162		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCGATTGCTGATTGCTAGGAGGGCTAAGAGCCG	ATTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5369	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_163	100	9606				MYC1_163	CRISPRko	CDS	MYC1_163		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAATAG	GGCTCTTAGCCCTCCTAGCAATCAGCAATAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	2	6	265	112	296	enPAM+GB	0.7987	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_163	100	9606				MYC1_163	CRISPRko	CDS	MYC1_163		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCTATTGCTGATTGCTAGGAGGGCTAAGAGCCG	ATTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5186	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_164	100	9606				MYC1_164	CRISPRko	CDS	MYC1_164		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAATGG	GGCTCTTAGCCCTCCTAGCAATCAGCAATGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7546	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_164	100	9606				MYC1_164	CRISPRko	CDS	MYC1_164		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCCATTGCTGATTGCTAGGAGGGCTAAGAGCCG	ATTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.3443	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_165	100	9606				MYC1_165	CRISPRko	CDS	MYC1_165		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAACTG	GGCTCTTAGCCCTCCTAGCAATCAGCAACTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	0	1	3	6	266	113	295	enPAM+GB	0.7910	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_165	100	9606				MYC1_165	CRISPRko	CDS	MYC1_165		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCAGTTGCTGATTGCTAGGAGGGCTAAGAGCCG	GTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5381	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_166	100	9606				MYC1_166	CRISPRko	CDS	MYC1_166		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAACCG	GGCTCTTAGCCCTCCTAGCAATCAGCAACCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	1	3	6	265	113	295	enPAM+GB	0.7969	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_166	100	9606				MYC1_166	CRISPRko	CDS	MYC1_166		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCGGTTGCTGATTGCTAGGAGGGCTAAGAGCCG	GTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5408	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_167	100	9606				MYC1_167	CRISPRko	CDS	MYC1_167		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAACAG	GGCTCTTAGCCCTCCTAGCAATCAGCAACAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	3	6	265	112	295	enPAM+GB	0.7910	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_167	100	9606				MYC1_167	CRISPRko	CDS	MYC1_167		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCTGTTGCTGATTGCTAGGAGGGCTAAGAGCCG	GTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5333	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_168	100	9606				MYC1_168	CRISPRko	CDS	MYC1_168		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAACGG	GGCTCTTAGCCCTCCTAGCAATCAGCAACGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7891	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_168	100	9606				MYC1_168	CRISPRko	CDS	MYC1_168		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCCGTTGCTGATTGCTAGGAGGGCTAAGAGCCG	GTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.3801	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_169	100	9606				MYC1_169	CRISPRko	CDS	MYC1_169		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAAATG	GGCTCTTAGCCCTCCTAGCAATCAGCAAATGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	1	3	6	265	113	295	enPAM+GB	0.7803	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_169	100	9606				MYC1_169	CRISPRko	CDS	MYC1_169		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCATTTGCTGATTGCTAGGAGGGCTAAGAGCCG	TTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.7792	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_170	100	9606				MYC1_170	CRISPRko	CDS	MYC1_170		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAAACG	GGCTCTTAGCCCTCCTAGCAATCAGCAAACGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	1	3	6	264	113	295	enPAM+GB	0.7871	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_170	100	9606				MYC1_170	CRISPRko	CDS	MYC1_170		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCGTTTGCTGATTGCTAGGAGGGCTAAGAGCCG	TTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.7819	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_171	100	9606				MYC1_171	CRISPRko	CDS	MYC1_171		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAAAAG	GGCTCTTAGCCCTCCTAGCAATCAGCAAAAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	2	3	6	264	112	294	enPAM+GB	0.7803	1	2	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_171	100	9606				MYC1_171	CRISPRko	CDS	MYC1_171		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCTTTTGCTGATTGCTAGGAGGGCTAAGAGCC	TTTT	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.6108	3	1	1.0	1.0	2	1	4	Previously skipped due to: Outside Target Window: 0-80%; Outside Target Window: 5-65%
MYC1_171	100	9606				MYC1_171	CRISPRko	CDS	MYC1_171		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCTTTTGCTGATTGCTAGGAGGGCTAAGAGCCG	TTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.7789	2	3	1.0	1.0	3			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_172	100	9606				MYC1_172	CRISPRko	CDS	MYC1_172		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAAAGG	GGCTCTTAGCCCTCCTAGCAATCAGCAAAGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	2	2	6	264	112	295	enPAM+GB	0.7503	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_172	100	9606				MYC1_172	CRISPRko	CDS	MYC1_172		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCCTTTGCTGATTGCTAGGAGGGCTAAGAGCCG	TTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.7172	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_173	100	9606				MYC1_173	CRISPRko	CDS	MYC1_173		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAAGTG	GGCTCTTAGCCCTCCTAGCAATCAGCAAGTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	1	3	6	265	113	295	enPAM+GB	0.7645	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_173	100	9606				MYC1_173	CRISPRko	CDS	MYC1_173		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCACTTGCTGATTGCTAGGAGGGCTAAGAGCCG	CTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5342	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_174	100	9606				MYC1_174	CRISPRko	CDS	MYC1_174		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAAGCG	GGCTCTTAGCCCTCCTAGCAATCAGCAAGCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	1	3	6	265	113	295	enPAM+GB	0.7821	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_174	100	9606				MYC1_174	CRISPRko	CDS	MYC1_174		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCGCTTGCTGATTGCTAGGAGGGCTAAGAGCCG	CTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5369	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_175	100	9606				MYC1_175	CRISPRko	CDS	MYC1_175		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAAGAG	GGCTCTTAGCCCTCCTAGCAATCAGCAAGAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	2	3	6	264	112	295	enPAM+GB	0.7655	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_175	100	9606				MYC1_175	CRISPRko	CDS	MYC1_175		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCTCTTGCTGATTGCTAGGAGGGCTAAGAGCCG	CTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5294	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_176	100	9606				MYC1_176	CRISPRko	CDS	MYC1_176		enAsCas12a	5	1	1	5.20.100		sense	30	TTGCTGATTGCTAGGAGGGCTAA	ATGACCCCTTGCTGATTGCTAGGAGGGCTAAGAG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	0	0	1	0	9	2	11	6	294	115	314	enPAM+GB	0.4829	2	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYC1_176	100	9606				MYC1_176	CRISPRko	CDS	MYC1_176		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAAGGG	GGCTCTTAGCCCTCCTAGCAATCAGCAAGGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7039	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_176	100	9606				MYC1_176	CRISPRko	CDS	MYC1_176		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCCCTTGCTGATTGCTAGGAGGGCTAAGAGCCG	CTTG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.3761	3	3	1.0	1.0	3			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_177	100	9606				MYC1_177	CRISPRko	CDS	MYC1_177		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGTTG	GGCTCTTAGCCCTCCTAGCAATCAGCAGTTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	0	2	2	6	266	112	296	enPAM+GB	0.7316	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_178	100	9606				MYC1_178	CRISPRko	CDS	MYC1_178		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGTCG	GGCTCTTAGCCCTCCTAGCAATCAGCAGTCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	2	6	265	112	296	enPAM+GB	0.7612	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_179	100	9606				MYC1_179	CRISPRko	CDS	MYC1_179		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGTAG	GGCTCTTAGCCCTCCTAGCAATCAGCAGTAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	2	6	265	112	296	enPAM+GB	0.7739	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_180	100	9606				MYC1_180	CRISPRko	CDS	MYC1_180		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGTGG	GGCTCTTAGCCCTCCTAGCAATCAGCAGTGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	2	6	265	112	296	enPAM+GB	0.7299	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_181	100	9606				MYC1_181	CRISPRko	CDS	MYC1_181		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGCTG	GGCTCTTAGCCCTCCTAGCAATCAGCAGCTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	0	1	3	6	266	113	296	enPAM+GB	0.7508	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_182	100	9606				MYC1_182	CRISPRko	CDS	MYC1_182		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGCCG	GGCTCTTAGCCCTCCTAGCAATCAGCAGCCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	0	1	3	6	266	113	296	enPAM+GB	0.7508	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_183	100	9606				MYC1_183	CRISPRko	CDS	MYC1_183		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGCAG	GGCTCTTAGCCCTCCTAGCAATCAGCAGCAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	1	2	6	265	113	296	enPAM+GB	0.7582	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_184	100	9606				MYC1_184	CRISPRko	CDS	MYC1_184		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGCGG	GGCTCTTAGCCCTCCTAGCAATCAGCAGCGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7209	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_185	100	9606				MYC1_185	CRISPRko	CDS	MYC1_185		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGATG	GGCTCTTAGCCCTCCTAGCAATCAGCAGATGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	1	4	6	265	113	295	enPAM+GB	0.7584	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_186	100	9606				MYC1_186	CRISPRko	CDS	MYC1_186		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGACG	GGCTCTTAGCCCTCCTAGCAATCAGCAGACGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	1	4	6	265	113	295	enPAM+GB	0.7750	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_186	100	9606				MYC1_186	CRISPRko	CDS	MYC1_186		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGAGGGCTAAG	TGACCGTCTGCTGATTGCTAGGAGGGCTAAGAGC	CGTC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	8	2	11	6	259	113	286	enPAM+GB	0.3394	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGAGGGCTAAG (for target MYC1_139)
MYC1_187	100	9606				MYC1_187	CRISPRko	CDS	MYC1_187		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCTTCTGCTGATTGCTAGGAGGGCTAAGAGCC	TTCT	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.4704	3	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_187	100	9606				MYC1_187	CRISPRko	CDS	MYC1_187		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGAGGGCTAAG	TGACCTTCTGCTGATTGCTAGGAGGGCTAAGAGC	CTTC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	8	2	11	6	259	113	286	enPAM+GB	0.5362	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGAGGGCTAAG (for target MYC1_139)
MYC1_187	100	9606				MYC1_187	CRISPRko	CDS	MYC1_187		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGAAG	GGCTCTTAGCCCTCCTAGCAATCAGCAGAAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	1	2	6	265	113	295	enPAM+GB	0.7584	1	3	1.0	1.0	3			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_188	100	9606				MYC1_188	CRISPRko	CDS	MYC1_188		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGAGG	GGCTCTTAGCCCTCCTAGCAATCAGCAGAGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.7344	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_189	100	9606				MYC1_189	CRISPRko	CDS	MYC1_189		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGAGGGCTAAG	TGACCACCTGCTGATTGCTAGGAGGGCTAAGAGC	CACC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	8	2	11	6	259	113	286	enPAM+GB	0.3209	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGAGGGCTAAG (for target MYC1_139)
MYC1_189	100	9606				MYC1_189	CRISPRko	CDS	MYC1_189		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGGTG	GGCTCTTAGCCCTCCTAGCAATCAGCAGGTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	0	1	2	6	266	113	296	enPAM+GB	0.7451	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_190	100	9606				MYC1_190	CRISPRko	CDS	MYC1_190		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGAGGGCTAAG	TGACCGCCTGCTGATTGCTAGGAGGGCTAAGAGC	CGCC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	8	2	11	6	259	113	286	enPAM+GB	0.3259	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGAGGGCTAAG (for target MYC1_139)
MYC1_190	100	9606				MYC1_190	CRISPRko	CDS	MYC1_190		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGGCG	GGCTCTTAGCCCTCCTAGCAATCAGCAGGCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	1	2	6	265	113	296	enPAM+GB	0.7530	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_191	100	9606				MYC1_191	CRISPRko	CDS	MYC1_191		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGAGGGCTAAG	TGACCTCCTGCTGATTGCTAGGAGGGCTAAGAGC	CTCC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	8	2	11	6	259	113	286	enPAM+GB	0.4210	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGAGGGCTAAG (for target MYC1_139)
MYC1_191	100	9606				MYC1_191	CRISPRko	CDS	MYC1_191		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGGAG	GGCTCTTAGCCCTCCTAGCAATCAGCAGGAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	2	2	6	265	112	296	enPAM+GB	0.7496	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_192	100	9606				MYC1_192	CRISPRko	CDS	MYC1_192		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCAGGGG	GGCTCTTAGCCCTCCTAGCAATCAGCAGGGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	2	1	6	265	112	296	enPAM+GB	0.6725	1	2	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCATTTG (for target MYC1_129)
MYC1_192	100	9606				MYC1_192	CRISPRko	CDS	MYC1_192		enAsCas12a	5	1	1	5.20.100		sense	31	TGCTGATTGCTAGGAGGGCTAAG	TGACCCCCTGCTGATTGCTAGGAGGGCTAAGAGC	CCCC	1	30	37	81.1	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	8	2	11	6	259	113	286	enPAM+GB	0.3299	3	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with TGCTGATTGCTAGGAGGGCTAAG (for target MYC1_139)
MYC1_192	100	9606				MYC1_192	CRISPRko	CDS	MYC1_192		enAsCas12a	5	1	1	5.20.100		sense	30	CTGCTGATTGCTAGGAGGGCTAA	ATGACCCCCTGCTGATTGCTAGGAGGGCTAAGAG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	1	0	0	0	15	6	9	5	269	83	224	enPAM+GB	0.4933	2	3	1.0	1.0	3	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYC1_193	100	9606				MYC1_193	CRISPRko	CDS	MYC1_193		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTTTG	GGCTCTTAGCCCTCCTAGCAATCAGCGTTTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	1	3	688	399	1087	enPAM+GB	0.7366	1	1	1.0	1.0	1	1	1	
MYC1_194	100	9606				MYC1_194	CRISPRko	CDS	MYC1_194		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTTCG	GGCTCTTAGCCCTCCTAGCAATCAGCGTTCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	1	3	688	399	1087	enPAM+GB	0.7367	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_195	100	9606				MYC1_195	CRISPRko	CDS	MYC1_195		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTTAG	GGCTCTTAGCCCTCCTAGCAATCAGCGTTAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	1	3	688	399	1086	enPAM+GB	0.7586	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_196	100	9606				MYC1_196	CRISPRko	CDS	MYC1_196		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTTGG	GGCTCTTAGCCCTCCTAGCAATCAGCGTTGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	0	3	688	399	1087	enPAM+GB	0.7054	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_197	100	9606				MYC1_197	CRISPRko	CDS	MYC1_197		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTCTG	GGCTCTTAGCCCTCCTAGCAATCAGCGTCTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	2	3	689	399	1087	enPAM+GB	0.7933	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_198	100	9606				MYC1_198	CRISPRko	CDS	MYC1_198		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTCCG	GGCTCTTAGCCCTCCTAGCAATCAGCGTCCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	2	3	689	399	1087	enPAM+GB	0.7933	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_199	100	9606				MYC1_199	CRISPRko	CDS	MYC1_199		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTCAG	GGCTCTTAGCCCTCCTAGCAATCAGCGTCAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	1	3	689	399	1086	enPAM+GB	0.8006	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_200	100	9606				MYC1_200	CRISPRko	CDS	MYC1_200		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTCGG	GGCTCTTAGCCCTCCTAGCAATCAGCGTCGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	0	3	689	399	1087	enPAM+GB	0.7634	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_201	100	9606				MYC1_201	CRISPRko	CDS	MYC1_201		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTATG	GGCTCTTAGCCCTCCTAGCAATCAGCGTATGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	0	2	3	689	399	1086	enPAM+GB	0.7884	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_202	100	9606				MYC1_202	CRISPRko	CDS	MYC1_202		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTACG	GGCTCTTAGCCCTCCTAGCAATCAGCGTACGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	0	2	3	689	399	1086	enPAM+GB	0.8089	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_203	100	9606				MYC1_203	CRISPRko	CDS	MYC1_203		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGAGGGCTAAG	TGACCTTACGCTGATTGCTAGGAGGGCTAAGAGC	CTTA	1	30	37	81.1	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	2	0	2	3	689	396	1089	enPAM+GB	0.5665	2	1	1.0	1.0	1	1	4	Previously skipped due to: Outside Target Window: 0-80%; Outside Target Window: 5-65%
MYC1_203	100	9606				MYC1_203	CRISPRko	CDS	MYC1_203		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTAAG	GGCTCTTAGCCCTCCTAGCAATCAGCGTAAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	1	3	688	399	1086	enPAM+GB	0.7957	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_204	100	9606				MYC1_204	CRISPRko	CDS	MYC1_204		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTAGG	GGCTCTTAGCCCTCCTAGCAATCAGCGTAGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	1	3	689	399	1086	enPAM+GB	0.7683	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_205	100	9606				MYC1_205	CRISPRko	CDS	MYC1_205		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTGTG	GGCTCTTAGCCCTCCTAGCAATCAGCGTGTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	2	3	689	399	1087	enPAM+GB	0.7597	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_206	100	9606				MYC1_206	CRISPRko	CDS	MYC1_206		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGAGGGCTAAG	TGACCGCACGCTGATTGCTAGGAGGGCTAAGAGC	CGCA	1	30	37	81.1	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	2	0	2	3	689	396	1089	enPAM+GB	0.2612	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGAGGGCTAAG (for target MYC1_203)
MYC1_206	100	9606				MYC1_206	CRISPRko	CDS	MYC1_206		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTGCG	GGCTCTTAGCCCTCCTAGCAATCAGCGTGCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	0	2	3	689	399	1086	enPAM+GB	0.7704	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_207	100	9606				MYC1_207	CRISPRko	CDS	MYC1_207		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGAGGGCTAAG	TGACCTCACGCTGATTGCTAGGAGGGCTAAGAGC	CTCA	1	30	37	81.1	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	2	0	2	3	689	396	1089	enPAM+GB	0.2977	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGAGGGCTAAG (for target MYC1_203)
MYC1_207	100	9606				MYC1_207	CRISPRko	CDS	MYC1_207		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTGAG	GGCTCTTAGCCCTCCTAGCAATCAGCGTGAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	1	3	689	399	1086	enPAM+GB	0.7670	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_208	100	9606				MYC1_208	CRISPRko	CDS	MYC1_208		enAsCas12a	5	1	1	5.20.100		sense	30	ACGCTGATTGCTAGGAGGGCTAA	ATGACCCCACGCTGATTGCTAGGAGGGCTAAGAG	CCCC	1	29	37	78.4	NO_SITE (6 zeros)	0	0	0	0	0	0	0	0	0	1	2	5	4	94	33	96	enPAM+GB	0.5207	2	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYC1_208	100	9606				MYC1_208	CRISPRko	CDS	MYC1_208		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGTGGG	GGCTCTTAGCCCTCCTAGCAATCAGCGTGGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	1	3	689	399	1086	enPAM+GB	0.7457	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_209	100	9606				MYC1_209	CRISPRko	CDS	MYC1_209		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCTTG	GGCTCTTAGCCCTCCTAGCAATCAGCGCTTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	2	3	688	399	1086	enPAM+GB	0.7484	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_210	100	9606				MYC1_210	CRISPRko	CDS	MYC1_210		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCTCG	GGCTCTTAGCCCTCCTAGCAATCAGCGCTCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	2	3	688	399	1086	enPAM+GB	0.7730	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_211	100	9606				MYC1_211	CRISPRko	CDS	MYC1_211		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCTAG	GGCTCTTAGCCCTCCTAGCAATCAGCGCTAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	2	3	688	399	1086	enPAM+GB	0.7957	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_212	100	9606				MYC1_212	CRISPRko	CDS	MYC1_212		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCTGG	GGCTCTTAGCCCTCCTAGCAATCAGCGCTGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	1	3	688	399	1086	enPAM+GB	0.7524	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_213	100	9606				MYC1_213	CRISPRko	CDS	MYC1_213		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCCTG	GGCTCTTAGCCCTCCTAGCAATCAGCGCCTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	3	3	689	399	1086	enPAM+GB	0.7823	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_214	100	9606				MYC1_214	CRISPRko	CDS	MYC1_214		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCCCG	GGCTCTTAGCCCTCCTAGCAATCAGCGCCCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	3	3	689	399	1086	enPAM+GB	0.7823	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_215	100	9606				MYC1_215	CRISPRko	CDS	MYC1_215		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCCAG	GGCTCTTAGCCCTCCTAGCAATCAGCGCCAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	1	3	688	399	1086	enPAM+GB	0.7896	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_216	100	9606				MYC1_216	CRISPRko	CDS	MYC1_216		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCCGG	GGCTCTTAGCCCTCCTAGCAATCAGCGCCGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	1	3	689	399	1086	enPAM+GB	0.7524	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_217	100	9606				MYC1_217	CRISPRko	CDS	MYC1_217		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCATG	GGCTCTTAGCCCTCCTAGCAATCAGCGCATGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	3	3	689	399	1086	enPAM+GB	0.7896	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_218	100	9606				MYC1_218	CRISPRko	CDS	MYC1_218		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCACG	GGCTCTTAGCCCTCCTAGCAATCAGCGCACGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	2	3	688	399	1086	enPAM+GB	0.7896	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_219	100	9606				MYC1_219	CRISPRko	CDS	MYC1_219		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGAGGGCTAAG	TGACCTTGCGCTGATTGCTAGGAGGGCTAAGAGC	CTTG	1	30	37	81.1	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	2	0	2	3	689	396	1089	enPAM+GB	0.4173	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGAGGGCTAAG (for target MYC1_203)
MYC1_219	100	9606				MYC1_219	CRISPRko	CDS	MYC1_219		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCAAG	GGCTCTTAGCCCTCCTAGCAATCAGCGCAAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	1	3	688	399	1086	enPAM+GB	0.7969	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_220	100	9606				MYC1_220	CRISPRko	CDS	MYC1_220		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCAGG	GGCTCTTAGCCCTCCTAGCAATCAGCGCAGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	1	3	688	399	1086	enPAM+GB	0.7596	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_221	100	9606				MYC1_221	CRISPRko	CDS	MYC1_221		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCGTG	GGCTCTTAGCCCTCCTAGCAATCAGCGCGTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	2	0	3	3	688	399	1086	enPAM+GB	0.7704	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_222	100	9606				MYC1_222	CRISPRko	CDS	MYC1_222		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCGCG	GGCTCTTAGCCCTCCTAGCAATCAGCGCGCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	2	0	3	3	688	399	1086	enPAM+GB	0.7676	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_223	100	9606				MYC1_223	CRISPRko	CDS	MYC1_223		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCGAG	GGCTCTTAGCCCTCCTAGCAATCAGCGCGAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	1	3	688	399	1086	enPAM+GB	0.7748	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_224	100	9606				MYC1_224	CRISPRko	CDS	MYC1_224		enAsCas12a	5	1	1	5.20.100		sense	30	GCGCTGATTGCTAGGAGGGCTAA	ATGACCCCGCGCTGATTGCTAGGAGGGCTAAGAG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	0	0	0	0	1	1	1	7	114	32	98	enPAM+GB	0.4933	2	1	1.0	1.0	1	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYC1_224	100	9606				MYC1_224	CRISPRko	CDS	MYC1_224		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGCGGG	GGCTCTTAGCCCTCCTAGCAATCAGCGCGGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	1	3	688	399	1086	enPAM+GB	0.7564	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_225	100	9606				MYC1_225	CRISPRko	CDS	MYC1_225		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCAATCGCTGATTGCTAGGAGGGCTAAGAGCC	AATC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.3448	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_225	100	9606				MYC1_225	CRISPRko	CDS	MYC1_225		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGATTG	GGCTCTTAGCCCTCCTAGCAATCAGCGATTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	1	3	688	399	1087	enPAM+GB	0.7492	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_226	100	9606				MYC1_226	CRISPRko	CDS	MYC1_226		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGATCG	GGCTCTTAGCCCTCCTAGCAATCAGCGATCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	2	3	688	399	1086	enPAM+GB	0.7845	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_227	100	9606				MYC1_227	CRISPRko	CDS	MYC1_227		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCTATCGCTGATTGCTAGGAGGGCTAAGAGCC	TATC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.4958	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_227	100	9606				MYC1_227	CRISPRko	CDS	MYC1_227		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGATAG	GGCTCTTAGCCCTCCTAGCAATCAGCGATAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	2	3	688	399	1086	enPAM+GB	0.7907	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_228	100	9606				MYC1_228	CRISPRko	CDS	MYC1_228		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGATGG	GGCTCTTAGCCCTCCTAGCAATCAGCGATGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	0	3	688	399	1087	enPAM+GB	0.7532	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_229	100	9606				MYC1_229	CRISPRko	CDS	MYC1_229		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGACTG	GGCTCTTAGCCCTCCTAGCAATCAGCGACTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	2	3	689	399	1087	enPAM+GB	0.7896	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_229	100	9606				MYC1_229	CRISPRko	CDS	MYC1_229		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCAGTCGCTGATTGCTAGGAGGGCTAAGAGCC	AGTC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.3538	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_230	100	9606				MYC1_230	CRISPRko	CDS	MYC1_230		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGACCG	GGCTCTTAGCCCTCCTAGCAATCAGCGACCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	3	3	689	399	1086	enPAM+GB	0.7896	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_231	100	9606				MYC1_231	CRISPRko	CDS	MYC1_231		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCTGTCGCTGATTGCTAGGAGGGCTAAGAGCC	TGTC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.4851	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_231	100	9606				MYC1_231	CRISPRko	CDS	MYC1_231		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGACAG	GGCTCTTAGCCCTCCTAGCAATCAGCGACAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	0	2	3	689	399	1086	enPAM+GB	0.7969	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_232	100	9606				MYC1_232	CRISPRko	CDS	MYC1_232		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCCGTCGCTGATTGCTAGGAGGGCTAAGAGCC	CGTC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.3796	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_232	100	9606				MYC1_232	CRISPRko	CDS	MYC1_232		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGACGG	GGCTCTTAGCCCTCCTAGCAATCAGCGACGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	1	3	689	399	1086	enPAM+GB	0.7818	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_233	100	9606				MYC1_233	CRISPRko	CDS	MYC1_233		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGAATG	GGCTCTTAGCCCTCCTAGCAATCAGCGAATGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	3	3	689	399	1086	enPAM+GB	0.7803	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_233	100	9606				MYC1_233	CRISPRko	CDS	MYC1_233		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCATTCGCTGATTGCTAGGAGGGCTAAGAGCCG	TTCG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5930	2	3	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_233	100	9606				MYC1_233	CRISPRko	CDS	MYC1_233		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCATTCGCTGATTGCTAGGAGGGCTAAGAGCC	ATTC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.5401	3	2	1.0	1.0	3			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_234	100	9606				MYC1_234	CRISPRko	CDS	MYC1_234		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGAACG	GGCTCTTAGCCCTCCTAGCAATCAGCGAACGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	3	3	689	399	1086	enPAM+GB	0.7969	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_234	100	9606				MYC1_234	CRISPRko	CDS	MYC1_234		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCGTTCGCTGATTGCTAGGAGGGCTAAGAGCCG	TTCG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5956	2	3	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_234	100	9606				MYC1_234	CRISPRko	CDS	MYC1_234		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCGTTCGCTGATTGCTAGGAGGGCTAAGAGCC	GTTC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.5715	3	2	1.0	1.0	3			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_235	100	9606				MYC1_235	CRISPRko	CDS	MYC1_235		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCTTTCGCTGATTGCTAGGAGGGCTAAGAGCC	TTTC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.8292	1	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_235	100	9606				MYC1_235	CRISPRko	CDS	MYC1_235		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGAAAG	GGCTCTTAGCCCTCCTAGCAATCAGCGAAAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	1	3	689	399	1086	enPAM+GB	0.7803	2	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_235	100	9606				MYC1_235	CRISPRko	CDS	MYC1_235		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCTTTCGCTGATTGCTAGGAGGGCTAAGAGCCG	TTCG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5927	3	3	1.0	1.0	3			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_236	100	9606				MYC1_236	CRISPRko	CDS	MYC1_236		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCCTTCGCTGATTGCTAGGAGGGCTAAGAGCC	CTTC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.5755	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_236	100	9606				MYC1_236	CRISPRko	CDS	MYC1_236		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGAAGG	GGCTCTTAGCCCTCCTAGCAATCAGCGAAGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	1	3	689	399	1086	enPAM+GB	0.7562	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_236	100	9606				MYC1_236	CRISPRko	CDS	MYC1_236		enAsCas12a	5	1	1	5.20.100		sense	33	CTGATTGCTAGGAGGGCTAAGAG	ACCCTTCGCTGATTGCTAGGAGGGCTAAGAGCCG	TTCG	1	32	37	86.5	NO_SITE (5 zeros)	0	0	0	0	0	2	0	0	0	10	2	15	5	151	55	141	enPAM+GB	0.5119	3	3	1.0	1.0	3			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CTGATTGCTAGGAGGGCTAAGAG (for target MYC1_137)
MYC1_237	100	9606				MYC1_237	CRISPRko	CDS	MYC1_237		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGAGTG	GGCTCTTAGCCCTCCTAGCAATCAGCGAGTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	0	2	3	689	399	1086	enPAM+GB	0.7670	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_238	100	9606				MYC1_238	CRISPRko	CDS	MYC1_238		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGAGCG	GGCTCTTAGCCCTCCTAGCAATCAGCGAGCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	1	0	2	3	689	399	1086	enPAM+GB	0.7748	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_239	100	9606				MYC1_239	CRISPRko	CDS	MYC1_239		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGAGAG	GGCTCTTAGCCCTCCTAGCAATCAGCGAGAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	1	3	688	399	1086	enPAM+GB	0.7714	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_240	100	9606				MYC1_240	CRISPRko	CDS	MYC1_240		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGAGGG	GGCTCTTAGCCCTCCTAGCAATCAGCGAGGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	1	3	689	399	1086	enPAM+GB	0.7529	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_240	100	9606				MYC1_240	CRISPRko	CDS	MYC1_240		enAsCas12a	5	1	1	5.20.100		sense	30	TCGCTGATTGCTAGGAGGGCTAA	ATGACCCCTCGCTGATTGCTAGGAGGGCTAAGAG	CCCC	1	29	37	78.4	NO_SITE (5 zeros)	0	0	0	0	0	1	0	0	0	7	2	10	5	703	404	1089	enPAM+GB	0.5082	2	2	1.0	1.0	2	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYC1_241	100	9606				MYC1_241	CRISPRko	CDS	MYC1_241		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCAACCGCTGATTGCTAGGAGGGCTAAGAGCC	AACC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.3565	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_241	100	9606				MYC1_241	CRISPRko	CDS	MYC1_241		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGTTG	GGCTCTTAGCCCTCCTAGCAATCAGCGGTTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	1	3	688	399	1087	enPAM+GB	0.7342	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_242	100	9606				MYC1_242	CRISPRko	CDS	MYC1_242		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGTCG	GGCTCTTAGCCCTCCTAGCAATCAGCGGTCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	1	3	688	399	1087	enPAM+GB	0.7459	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_243	100	9606				MYC1_243	CRISPRko	CDS	MYC1_243		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCTACCGCTGATTGCTAGGAGGGCTAAGAGCC	TACC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.5053	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_243	100	9606				MYC1_243	CRISPRko	CDS	MYC1_243		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGTAG	GGCTCTTAGCCCTCCTAGCAATCAGCGGTAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	2	3	688	399	1086	enPAM+GB	0.7683	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_244	100	9606				MYC1_244	CRISPRko	CDS	MYC1_244		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCCACCGCTGATTGCTAGGAGGGCTAAGAGCC	CACC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.4045	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_244	100	9606				MYC1_244	CRISPRko	CDS	MYC1_244		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGTGG	GGCTCTTAGCCCTCCTAGCAATCAGCGGTGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	0	3	688	399	1087	enPAM+GB	0.7057	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_245	100	9606				MYC1_245	CRISPRko	CDS	MYC1_245		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGCTG	GGCTCTTAGCCCTCCTAGCAATCAGCGGCTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	2	3	689	399	1087	enPAM+GB	0.7356	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_245	100	9606				MYC1_245	CRISPRko	CDS	MYC1_245		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCAGCCGCTGATTGCTAGGAGGGCTAAGAGCC	AGCC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.3614	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_246	100	9606				MYC1_246	CRISPRko	CDS	MYC1_246		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGCCG	GGCTCTTAGCCCTCCTAGCAATCAGCGGCCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	2	3	689	399	1087	enPAM+GB	0.7356	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_247	100	9606				MYC1_247	CRISPRko	CDS	MYC1_247		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGCAG	GGCTCTTAGCCCTCCTAGCAATCAGCGGCAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	3	3	689	399	1086	enPAM+GB	0.7428	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_247	100	9606				MYC1_247	CRISPRko	CDS	MYC1_247		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCTGCCGCTGATTGCTAGGAGGGCTAAGAGCC	TGCC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.4823	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_248	100	9606				MYC1_248	CRISPRko	CDS	MYC1_248		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCCGCCGCTGATTGCTAGGAGGGCTAAGAGCC	CGCC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.3998	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_248	100	9606				MYC1_248	CRISPRko	CDS	MYC1_248		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGCGG	GGCTCTTAGCCCTCCTAGCAATCAGCGGCGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	0	3	689	399	1087	enPAM+GB	0.6966	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_249	100	9606				MYC1_249	CRISPRko	CDS	MYC1_249		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGATG	GGCTCTTAGCCCTCCTAGCAATCAGCGGATGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	2	0	3	3	688	399	1086	enPAM+GB	0.7490	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_249	100	9606				MYC1_249	CRISPRko	CDS	MYC1_249		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCATCCGCTGATTGCTAGGAGGGCTAAGAGCC	ATCC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.4534	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_250	100	9606				MYC1_250	CRISPRko	CDS	MYC1_250		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGACG	GGCTCTTAGCCCTCCTAGCAATCAGCGGACGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	2	0	3	3	688	399	1086	enPAM+GB	0.7597	1	2	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_250	100	9606				MYC1_250	CRISPRko	CDS	MYC1_250		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGAGGGCTAAG	TGACCGTCCGCTGATTGCTAGGAGGGCTAAGAGC	CGTC	1	30	37	81.1	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	2	0	2	3	689	396	1089	enPAM+GB	0.3329	3	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGAGGGCTAAG (for target MYC1_203)
MYC1_250	100	9606				MYC1_250	CRISPRko	CDS	MYC1_250		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCGTCCGCTGATTGCTAGGAGGGCTAAGAGCC	GTCC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.4781	2	3	1.0	1.0	3			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_251	100	9606				MYC1_251	CRISPRko	CDS	MYC1_251		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCTTCCGCTGATTGCTAGGAGGGCTAAGAGCC	TTCC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.7332	2	2	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_251	100	9606				MYC1_251	CRISPRko	CDS	MYC1_251		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGAGGGCTAAG	TGACCTTCCGCTGATTGCTAGGAGGGCTAAGAGC	CTTC	1	30	37	81.1	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	2	0	2	3	689	396	1089	enPAM+GB	0.5297	3	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGAGGGCTAAG (for target MYC1_203)
MYC1_251	100	9606				MYC1_251	CRISPRko	CDS	MYC1_251		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGAAG	GGCTCTTAGCCCTCCTAGCAATCAGCGGAAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	2	3	688	399	1086	enPAM+GB	0.7563	1	3	1.0	1.0	3			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_252	100	9606				MYC1_252	CRISPRko	CDS	MYC1_252		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCCTCCGCTGATTGCTAGGAGGGCTAAGAGCC	CTCC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.5014	2	1	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_252	100	9606				MYC1_252	CRISPRko	CDS	MYC1_252		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGAGG	GGCTCTTAGCCCTCCTAGCAATCAGCGGAGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	2	0	1	3	688	399	1086	enPAM+GB	0.7101	1	2	1.0	1.0	2			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_253	100	9606				MYC1_253	CRISPRko	CDS	MYC1_253		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGGTG	GGCTCTTAGCCCTCCTAGCAATCAGCGGGTGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	2	3	689	399	1087	enPAM+GB	0.7298	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_253	100	9606				MYC1_253	CRISPRko	CDS	MYC1_253		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCACCCGCTGATTGCTAGGAGGGCTAAGAGCC	ACCC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.3487	2	3	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_253	100	9606				MYC1_253	CRISPRko	CDS	MYC1_253		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGAGGGCTAAG	TGACCACCCGCTGATTGCTAGGAGGGCTAAGAGC	CACC	1	30	37	81.1	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	2	0	2	3	689	396	1089	enPAM+GB	0.3061	3	2	1.0	1.0	3			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGAGGGCTAAG (for target MYC1_203)
MYC1_254	100	9606				MYC1_254	CRISPRko	CDS	MYC1_254		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGGCG	GGCTCTTAGCCCTCCTAGCAATCAGCGGGCGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	1	0	3	3	689	399	1086	enPAM+GB	0.7377	1	1	1.0	1.0	1			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_254	100	9606				MYC1_254	CRISPRko	CDS	MYC1_254		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGAGGGCTAAG	TGACCGCCCGCTGATTGCTAGGAGGGCTAAGAGC	CGCC	1	30	37	81.1	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	2	0	2	3	689	396	1089	enPAM+GB	0.3110	2	2	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGAGGGCTAAG (for target MYC1_203)
MYC1_255	100	9606				MYC1_255	CRISPRko	CDS	MYC1_255		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCTCCCGCTGATTGCTAGGAGGGCTAAGAGCC	TCCC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.5150	2	2	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_255	100	9606				MYC1_255	CRISPRko	CDS	MYC1_255		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGAGGGCTAAG	TGACCTCCCGCTGATTGCTAGGAGGGCTAAGAGC	CTCC	1	30	37	81.1	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	2	0	2	3	689	396	1089	enPAM+GB	0.4061	3	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGAGGGCTAAG (for target MYC1_203)
MYC1_255	100	9606				MYC1_255	CRISPRko	CDS	MYC1_255		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGGAG	GGCTCTTAGCCCTCCTAGCAATCAGCGGGAGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	1	0	2	0	2	3	688	399	1086	enPAM+GB	0.7342	1	3	1.0	1.0	3			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
MYC1_256	100	9606				MYC1_256	CRISPRko	CDS	MYC1_256		enAsCas12a	5	1	1	5.20.100		sense	32	GCTGATTGCTAGGAGGGCTAAGA	GACCCCCCGCTGATTGCTAGGAGGGCTAAGAGCC	CCCC	1	31	37	83.8	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	0	2	4	209	62	195	enPAM+GB	0.4134	3	2	1.0	1.0	1			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with GCTGATTGCTAGGAGGGCTAAGA (for target MYC1_171)
MYC1_256	100	9606				MYC1_256	CRISPRko	CDS	MYC1_256		enAsCas12a	5	1	1	5.20.100		sense	31	CGCTGATTGCTAGGAGGGCTAAG	TGACCCCCCGCTGATTGCTAGGAGGGCTAAGAGC	CCCC	1	30	37	81.1	NO_SITE (9 zeros)	0	0	0	0	0	0	0	0	0	2	0	2	3	689	396	1089	enPAM+GB	0.3150	4	1	1.0	1.0	2			Outside Target Window: 0-80%; Outside Target Window: 5-65%; Sequence Conflict with CGCTGATTGCTAGGAGGGCTAAG (for target MYC1_203)
MYC1_256	100	9606				MYC1_256	CRISPRko	CDS	MYC1_256		enAsCas12a	5	1	1	5.20.100		sense	30	CCGCTGATTGCTAGGAGGGCTAA	ATGACCCCCCGCTGATTGCTAGGAGGGCTAAGAG	CCCC	1	29	37	78.4	NO_SITE (7 zeros)	0	0	0	0	0	0	0	0	0	4	2	3	2	113	26	84	enPAM+GB	0.4984	2	3	1.0	1.0	3	1	3	Previously skipped due to: Outside Target Window: 5-65%
MYC1_256	100	9606				MYC1_256	CRISPRko	CDS	MYC1_256		enAsCas12a	5	1	1	5.20.100		antisense	8	GCCCTCCTAGCAATCAGCGGGGG	GGCTCTTAGCCCTCCTAGCAATCAGCGGGGGGTC	CTTA	1	7	37	18.9	NO_SITE (7 zeros)	0	0	0	0	0	0	0	2	0	1	0	1	3	689	399	1086	enPAM+GB	0.7135	1	4	1.0	1.0	4			Sequence Conflict with GCCCTCCTAGCAATCAGCGTTTG (for target MYC1_193)
