ID   MYSA_HUMAN     STANDARD;      PRT;  1939 AA.
AC   P13533;
DT   01-JAN-1990 (REL. 13, CREATED)
DT   01-JUN-1994 (REL. 29, LAST SEQUENCE UPDATE)
DT   01-JUN-1994 (REL. 29, LAST ANNOTATION UPDATE)
DE   MYOSIN HEAVY CHAIN, CARDIAC MUSCLE ALPHA ISOFORM.
GN   MYH6 OR MYHCA.
OS   HOMO SAPIENS (HUMAN).
OC   EUKARYOTA; METAZOA; CHORDATA; VERTEBRATA; TETRAPODA; MAMMALIA;
OC   EUTHERIA; PRIMATES.
RN   [1]
RP   SEQUENCE FROM N.A.
RX   MEDLINE; 92133665.
RA   MATSUOKA R., BEISEL K.W., FURUTANI M., ARAI S., TAKAO A.;
RL   AM. J. MED. GENET. 41:537-547(1991).
RN   [2]
RP   SEQUENCE OF 1407-1939 FROM N.A.
RX   MEDLINE; 88299163.
RA   KURABAYASHI M., TSUCHIMOCHI H., KOMURO I., TAKAKU F., YAZAKI Y.;
RL   J. CLIN. INVEST. 82:524-531(1988).
CC   -!- FUNCTION: MUSCLE CONTRACTION.
CC   -!- SUBUNIT: MUSCLE MYOSIN IS A HEXAMERIC PROTEIN THAT CONSISTS OF 2
CC       HEAVY CHAIN SUBUNITS (MHC), 2 ALKALI LIGHT CHAIN SUBUNITS (MLC)
CC       AND 2 REGULATORY LIGHT CHAIN SUBUNITS (MLC-2).
CC   -!- SUBCELLULAR LOCATION: THICK FILAMENTS OF THE MYOFIBRILS.
CC   -!- DOMAIN: THE RODLIKE TAIL SEQUENCE IS HIGHLY REPETITIVE, SHOWING
CC       CYCLES OF A 28-RESIDUE REPEAT PATTERN COMPOSED OF 4 HEPTAPEPTIDES,
CC       CHARACTERISTIC FOR ALPHA-HELICAL COILED COILS.
CC   -!- PTM: TWO CYSTEINE RESIDUES IN THE S1 DOMAIN ARE SELECTIVELY
CC       ALKYLATED AND ARE REQUIRED FOR MYOSIN ATPASE ACTIVITY.
CC   -!- EACH MYOSIN HEAVY CHAIN CAN BE SPLIT INTO 1 LIGHT MEROMYOSIN (LMM)
CC       AND 1 HEAVY MEROMYOSIN (HMM). IT CAN LATTER BE SPLIT FURTHER INTO
CC       2 GLOBULAR SUBFRAGMENTS (S1) AND 1 ROD-SHAPED SUBFRAGMENT (S2).
CC   -!- THE CARDIAC ALPHA ISOFORM IS A 'FAST' ATPASE MYOSIN, WHILE THE
CC       BETA ISOFORM IS A 'SLOW' ATPASE.
CC   -!- SIMILARITY: THE PERIODICITIES OF HYDROPHOBIC AND CHARGED RESIDUES,
CC       WHICH DICTATE THE ALPHA-HELICAL COILED-COIL STRUCTURE ARE
CC       CONSERVED.
DR   EMBL; D00943; G219524; -.
DR   EMBL; M21664; G386972; -.
DR   PIR; A46762; A46762.
DR   PIR; A28908; A28908.
DR   HSSP; P24733; 1SCM.
DR   MIM; 160710; -.
KW   MYOSIN; MUSCLE PROTEIN; COILED COIL; THICK FILAMENT; ACTIN-BINDING;
KW   ATP-BINDING; METHYLATION; ALKYLATION; HEPTAD REPEAT PATTERN;
KW   MULTIGENE FAMILY.
FT   DOMAIN        1    840       GLOBULAR HEAD (S1).
FT   DOMAIN      841   1939       RODLIKE TAIL (S2 AND LMM DOMAINS).
FT   NP_BIND     178    185       ATP.
FT   DOMAIN      657    679       ACTIN-BINDING.
FT   DOMAIN      759    773       ACTIN-BINDING.
FT   MOD_RES     129    129       METHYLATION (TRI-) (POTENTIAL).
FT   MOD_RES     697    697       ALKYLATION (SH-1) (POTENTIAL).
FT   MOD_RES     707    707       ALKYLATION (SH-2) (POTENTIAL).
FT   CONFLICT   1533   1533       K -> N (IN REF. 2).
FT   CONFLICT   1540   1540       L -> M (IN REF. 2).
FT   CONFLICT   1577   1578       KL -> NV (IN REF. 2).
FT   CONFLICT   1705   1706       DR -> EQ (IN REF. 2).
FT   CONFLICT   1763   1763       H -> D (IN REF. 2).
FT   CONFLICT   1871   1871       D -> N (IN REF. 2).
FT   CONFLICT   1882   1882       R -> G (IN REF. 2).
FT   CONFLICT   1890   1890       Q -> R (IN REF. 2).
FT   CONFLICT   1933   1933       MISSING (IN REF. 2).
SQ   SEQUENCE   1939 AA;  223552 MW;  40812E5A CRC32;
     MTDAQMADFG AAAQYLRKSE KERLEAQTRP FDIRTECFVP DDKEEFVKAK ILSREGGKVI
     AETENGKTVT VKEDQVLQQN PPKFDKIEDM AMLTFLHEPA VLFNLKERYA AWMIYTYSGL
     FCVTVNPYKW LPVYNAEVVA AYRGKKRSEA PPHIFSISDN AYQYMLTDRE NQSILITGES
     GAGKTVNTKR VIQYFASIAA IGDRGKKDNA NANKGTLEDQ IIQANPALEA FGNAKTVRND
     NSSRFGKFIR IHFGATGKLA SADIETYLLE KSRVIFQLKA ERNYHIFYQI LSNKKPELLD
     MLLVTNNPYD YAFVSQGEVS VASIDDSEEL MATDSAFDVL GFTSEEKAGV YKLTGAIMHY
     GNMKFKQKQR EEQAEPDGTE DADKSAYLMG LNSADLLKGL CHPRVKVGNE YVTKGQSVQQ
     VYYSIGALAK AVYEKMFNWM VTRINATLET KQPRQYFIGV LDIAGFEIFD FNSFEQLCIN
     FTNEKLQQFF NHHMFVLEQE EYKKEGIEWT FIDFGMDLQA CIDLIEKPMG IMSILEEECM
     FPKATDMTFK AKLYDNHLGK SNNFQKPRNI KGKPEAHFSL IHYAGTVDYN ILGWLEKNKD
     PLNETVVGLY QKSSLKLMAT LFSSYATADT GDSGKSKGGK KKGSSFQTVS ALHRENLNKL
     MTNLRTTHPH FVRCIIPNER KAPGVMDNPL VMHQLRCNGV LEGIRICRKG FPNRILYGDF
     RQRYRILNPV AIPEGQFIDS RKGAEKLLSS LDIDHNQYKF GHTKVFFKAG LLGLLEEMRD
     ERLSRIITRI QAQARGQLMR IEFKKIVERR DALLVIQWNI RAFMGVKNWP WMKLYFKIKP
     LLKSAETEKE MATMKEEFGR IKETLEKSEA RRKELEEKMV SLLQEKNDLQ LQVQAEQDNL
     NDAEERCDQL IKNKIQLEAK VKEMNERLED EEEMNAELTA KKRKLEDECS ELKKDIDDLE
     LTLAKVEKEK HATENKVKNL TEEMAGLDEI IAKLTKEKKA LQEAHQQALD DLQAEEDKVN
     TLSKSKVKLE QQVDDLEGSL EQEKKVRMDL ERAKRKLEGD LKLTQESIMD LENDKLQLEE
     KLKKKEFDIN QQNSKIEDEQ VLALQLQKKL KENQARIEEL EEELEAERTA RAKVEKLRSD
     LSRELEEISE RLEEAGGATS VQIEMNKKRE AEFQKMRRDL EEATLQHEAT AAALRKKHAD
     SVAELGEQID NLQRVKQKLE KEKSEFKLEL DDVTSNMEQI IKAKANLEKV SRTLEDQANE
     YRVKLEEAQR SLNDFTTQRA KLQTENGELS RQLEEKEALI SQLTRGKLSY TQQMEDLKRQ
     LEEEGKAKNA LAHALQSARH DCDLLREQYE EETEAKAELQ RVLSKANSEV AQCRTKYETD
     AIQRTEELEE AKKKLAQRLQ DAEEAVEAVN AKCSSLEKTK HRLQNEIEDL MVDVERSNAA
     AAALDKKQRN FDKILAEWKQ KYEESQSELE SSQKEARSLS TELFKLKNAY EESLEHLETF
     KRENKNLQEE ISDLTEQLGE GGKNVHELEK VRKQLEVEKL ELQSALEEAE ASLEHEEGKI
     LRAQLEFNQI KAEIERKLAE KDEEMEQAKR NHQRVVDSLQ TSLDAETRSR NEVLRVKKKM
     EGDLNEMEIQ LSHANRMAAE AQKQVKSLQS LLKDTQIQLD DAVRANDDLK ENIAIVERRN
     NLLQAELEEL RAVVEQTERS RKLADRELIE TSERVQLLHS QNTSLINQKK KMDADLSQLQ
     SEVEEAVQEC RNAEEKAKKA ITHAAMMAEE LKKEQDTSAH LERMKKNMEQ TIKDLQHRLD
     EAEQIALKGG KKQLQKLEAR VRELEGELEA EQKRNAESVK GMRKSERRIK ELTYQTEEDK
     KNLLRLQDLV DKLQLKVKAY KRQAEEAEEQ ANTNLSKFRK VQHELDEAEE RADIAESQVN
     KLRAKSRDIG AKQKMHDEE
//
