ID   AB031077; SV 1; circular; other DNA; STD; SYN; 5680 BP.
XX
AC   AB031077;
XX
DT   14-JAN-2000 (Rel. 62, Created)
DT   24-JAN-2004 (Rel. 78, Last updated, Version 3)
XX
DE   Cloning vector pMG103 DNA, complete sequence.
XX
KW   .
XX
OS   Cloning vector pMG103
OC   other sequences; artificial sequences; vectors.
XX
OS   synthetic construct
OC   other sequences; artificial sequences.
XX
OS   Rhodopseudomonas palustris
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Rhodopseudomonas.
OG   Plasmid pMG101
XX
RN   [1]
RP   1-5680
RA   Inui M.;
RT   ;
RL   Submitted (16-AUG-1999) to the INSDC.
RL   Masayuki Inui, Research Institute of Innovative Technology for the Earth,
RL   Molecular Microbiology and Genetics Laboratory; 9-2, Kizugawadai, Soraku,
RL   Kizu, Kyoto 619-0292, Japan (E-mail:inui@rite.or.jp, Tel:81-774-75-2308,
RL   Fax:81-774-75-2321)
XX
RN   [2]
RX   DOI; 10.1128/AEM.66.1.54-63.2000.
RX   PUBMED; 10618203.
RA   Inui M., Roh J.H., Zahn K., Yukawa H.;
RT   "Sequence analysis of the cryptic plasmid pMG101 from Rhodopseudomonas
RT   palustris and construction of stable cloning vectors";
RL   Appl. Environ. Microbiol. 66(1):54-63(2000).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..5680
FT                   /organism="Cloning vector pMG103"
FT                   /focus
FT                   /mol_type="other DNA"
FT                   /db_xref="taxon:102152"
FT   source          1..27
FT                   /organism="synthetic construct"
FT                   /mol_type="other DNA"
FT                   /note="derived from cloning vector pHSG298"
FT                   /db_xref="taxon:32630"
FT   source          28..3080
FT                   /organism="Rhodopseudomonas palustris"
FT                   /plasmid="pMG101"
FT                   /mol_type="other DNA"
FT                   /note="derived from 3.0-kb XhoI-SalI fragment of pMG101"
FT                   /db_xref="taxon:1076"
FT   source          3081..5680
FT                   /organism="synthetic construct"
FT                   /mol_type="other DNA"
FT                   /note="derived from cloning vector pHSG298"
FT                   /db_xref="taxon:32630"
FT   CDS             3331..4146
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /product="aminoglucoside phosphotransferase"
FT                   /note="kanamycin-resistance"
FT                   /protein_id="BAA89407.1"
FT                   /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
FT                   KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
FT                   TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
FT                   SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
FT                   ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
FT   CDS             complement(4147..4455)
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="lacZ alpha"
FT                   /protein_id="BAA89408.1"
FT                   /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
FT                   LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF"
FT   misc_feature    4385..4441
FT                   /note="multiple cloning sites"
XX
SQ   Sequence 5680 BP; 1278 A; 1452 C; 1604 G; 1346 T; 0 other;
     gaggtctgcc tcgtgaagaa ggtgttgctc gagcgggtca tgaacaccct gccagatcga        60
     gaatcccgaa cttggcgcgc ggtattcttc aaggccctgc ctgaggtgat ggaggatttt       120
     gggggcgact ggctacaagc aactggcgaa cggcgaaatc cgttcgagcg ggtggaacag       180
     acgaagccaa taggaaggcc ataggcgcgt ttttatagac ttgaagcccg gaagtccgtc       240
     cacacccaaa aaatcgctgt gcgaggcgct gtggctgtcc tgtgttgcct tcgatgaaag       300
     gcgaaacttg ccctcatcat ggcagtcccc cctagtctgg tccttaaagc ggtcaagggc       360
     gatgccggag ggagcccttg agggcttgtg gggactaggc ttctttccct ttttcaatcg       420
     tctttttgaa gactgcttgt tctgaagatt ctgaaaagtg ttaggaagaa gtgttctgca       480
     atgttctgta gtgttctgtt ctatatagcg ccaccaagtc tgcggtagag cgctaccaag       540
     tctgcggaaa gccgccacca agtctgcggt agaagaagcg aaaacgccac caagtctgcg       600
     gtagagtgcc accaagtctg cggtggcggc ggaataagcc cttgaaatta ttgagtcccg       660
     ggaacagtaa ggcgccacca agtctgcgga gctaagtggt tgcgtttgcg gctacttttt       720
     gcgcacacgg atcgcaggta atgccagcac ggcggggtcg gctggttcga tggaaatgcc       780
     gtattgcgtg agcttgagag gcagctttgg cagcacttcc ttgatgtgtg tcagctcctc       840
     gatcagctgc gcccggaacg cgcgaggccg agtgaaacct tggccaaatt gttcgctgag       900
     agccttccaa ctcatggtca ccgggtggcg aatattgtga aggcgatagc ctagccagaa       960
     atacaaatcg agcttgcgag ctgagcctgc gaaggctcga accgcattag cattcaaggg      1020
     gagcgcacgc tgctgtaggc tctcaaagaa taccgggttg aacgtgacgg tactgggcca      1080
     aagcgagcgt tggtcaagat gcggcggtag ccacacctca acctcatcga acggattgac      1140
     ggttttggtt tttgccctca ccccgtccca cacactcacc cgcattgtgc aagccgccag      1200
     tgcgttaatt tgttctttga aggcggtgag cgggcctttg cgtcccccgc tgtccgaaaa      1260
     tcccatctcg cgcacaaagg cagtaaagct ctcggcgatt tcgatagtag gtgttttctg      1320
     ccggatggct tccgaacaaa ggtgcatcat caccagtcgg gcttttggcc cgaaaggcag      1380
     cggttgaagg attttcttgc cttctccgtc tcgcaggaat ccggcggaca aatccagcgc      1440
     catattgccc tgcttccgtt cgaactcgcg cacttcgaga ggttgacggt cgtaaggcat      1500
     accgcaaacg gcaagcacgc tgtgcaaatg ccgaacgtcg gcgggcgatg gcggtgagtt      1560
     ttcgataact tcgcggatga tggaacggcg gcggcgctcg cgtggcattg cgtcaagctc      1620
     agccttgcgc ttttcagcgg cggcctgttc ctcgtctcgc ttttgctggc gggtgatgag      1680
     cacgctgact tgggcatcaa acgcaaactt accccgcgct ttttcaagct cggcgcgcaa      1740
     atcttcatcc cggatcagcg attcgtcata cttgcccata ggcacagagt agctgatttg      1800
     ctgcggtctc cctaggcgcc tgcctgccta tagggcaatt acgtacagca ttatagcgtt      1860
     gtggcgttgt aacgctatag cattgcaatg ctaggacttg cgcttggccg tctcggagtg      1920
     gccgcgccta gcaatcacag catcgactcc ctcgacgatg aggtcatgca cctttttgcg      1980
     ctcgacaaag gcgatctctc gcagccgatc atgagccgct tggccaaata gatactggag      2040
     cgcactacag gagaggcttc agatggggga ggagcggctt ttggcgcgcg catcttcacc      2100
     accctggatt tcgacggagc cggagcgtca ggctccacct cgggggtgat ttctagccct      2160
     tcgatgatag gggggcgctt tttcggcggg ctcatgcgac ggctttcgtt ttgagtttgt      2220
     gctggatgga tctccagagt ccgcgggcct cctcggccgc cttgccgctg ggagcgtact      2280
     cggtgacccc ctgccctgcc gcgatggcat cctgaaagtc agccctagaa accatcaatg      2340
     gctctgcaag tgatcccagg gctgacaatc ccacggcggc ctcgctggcg cgcccgctgc      2400
     gggtgatcgg ggggcactgg ttgagaacaa acaggaatgg ccgctgcagc ttgagaagcg      2460
     cttggatggt cggccgggtt gcctgaatgt ctaggcgcgt cggcctagca ggcactaggg      2520
     agaggtcggc tgcctgcatg gcgagagagg tggcggtgct ggcgacaccc gggcaatcga      2580
     ggatggcgag ggtgtacccc ttccctgcca gcgccttcag gatttgaggg agctggggca      2640
     ccttgtcagg cggaagggca tcaacggcgg gcttctcccg ctgggcctga gcccgttgat      2700
     ctccccaagc tgcaagcgaa ccttgcgggt caaggtcgag ggcaatcacg gattcccctg      2760
     cctctgtggc cgcgacggca atggcagcag cgagggtagt ttttcccgcg ccgcccttct      2820
     gcgtgaccag agcaattgtc ttcatgcctg cactatagca ttaaggcact aaagcgtcaa      2880
     agcgccatag cggcatagcg gcatagcggc atagcgctaa aatgctatag cattattaaa      2940
     tacagcgcta cagcgctata atgctgcaac ggttaggacc gcaatttgcg ccccgggccg      3000
     gttgcgctat cgaccagctc aattaactgc tcgggctcgg acgcgaacca cgcgaagctg      3060
     ccccaagcca aggagtcgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag      3120
     ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg      3180
     atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa      3240
     aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct      3300
     gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc      3360
     tcgaagccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc      3420
     gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca      3480
     gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc      3540
     agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact      3600
     cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta      3660
     gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg      3720
     ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct      3780
     caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt      3840
     aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg      3900
     gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa      3960
     ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc      4020
     atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa      4080
     tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt      4140
     ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg      4200
     gacggcggct ttgttgaata aatcgcattc gccattcagg ctgcgcaact gttgggaagg      4260
     gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag      4320
     gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag      4380
     tgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt      4440
     cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca      4500
     acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca      4560
     cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc      4620
     attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggcgaa cttttgctga      4680
     gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa      4740
     gttcaaaatc agtaaccgtc agtgccgata agttcaaagt taaacctggt gttgatacca      4800
     acattgaaac gctgatcgaa aacgcgctga aaaacgctgc tgaatgtgcg agcttcttcc      4860
     gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct      4920
     cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg      4980
     tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc      5040
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga      5100
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct      5160
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg      5220
     gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag      5280
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat      5340
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac      5400
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac      5460
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc      5520
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt      5580
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc      5640
     ttttctacgg ggtctgacgc tcagtggaac gatccgtcga                            5680
//
