
                                 pepstats 



Function

   Protein statistics

Description

   pepstats outputs a report of simple protein sequence information
   including:

     * Molecular weight
     * Number of residues
     * Average residue weight
     * Charge
     * Isoelectric point
     * For each type of amino acid: number, molar percent, DayhoffStat
     * For each physico-chemical class of amino acid: number, molar
       percent
     * Probability of protein expression in E. coli inclusion bodies
     * Molar extinction coefficient (A280)
     * Extinction coefficient at 1 mg/ml (A280)

   DayhoffStat is the amino acid's molar percentage divided by the
   Dayhoff statistic. The Dayhoff statistic is the amino acid's relative
   occurence per 1000 aa normalised to 100 by rls@ebi.ac.uk (original
   work from 1993)

   The probability of expression in inclusion bodies is sometimes
   referred to as a type of solubility measure. If, however, a
   recombinant protein is expressed in Escherichia coli, it can be
   expressed as soluble in the cytosol or insoluble in inclusion bodies.
   If the Harrison model predicts a given protein to be probably
   expressed in includion bodies, this doesn't mean that it is not
   possible to get it soluble in the cytosol. One example: Thermatoga
   maritima cell divison protein FtsA with a C-terminal His-Tag has a 58%
   Harrison probability of being expressed in inclusion bodies. However,
   there was plenty of soluble protein in the E. coli cytosol (F. van den
   Ent and J. Lowe, EMBO J. 19, 5300-5307 2000). If the protein is
   expressed in inclusion bodies or not is not only dependent on the
   sequence, but also on many other factors, such as E. coli strain,
   incubation temperature, type of expression vector, strength of
   promoter and medium.

Usage

   Here is a sample session with pepstats


% pepstats 
Protein statistics
Input protein sequence(s): tsw:laci_ecoli
Pepstats program output file [laci_ecoli.pepstats]: 

   Go to the input files for this example
   Go to the output files for this example

Command line arguments

   Standard (Mandatory) qualifiers:
  [-sequence]          seqall     Protein sequence(s) filename and optional
                                  format, or reference (input USA)
  [-outfile]           outfile    [*.pepstats] Pepstats program output file

   Additional (Optional) qualifiers: (none)
   Advanced (Unprompted) qualifiers:
   -aadata             datafile   [Eamino.dat] Molecular weight data for amino
                                  acids
   -[no]termini        boolean    [Y] Include charge at N and C terminus

   Associated qualifiers:

   "-sequence" associated qualifiers
   -sbegin1            integer    Start of each sequence to be used
   -send1              integer    End of each sequence to be used
   -sreverse1          boolean    Reverse (if DNA)
   -sask1              boolean    Ask for begin/end/reverse
   -snucleotide1       boolean    Sequence is nucleotide
   -sprotein1          boolean    Sequence is protein
   -slower1            boolean    Make lower case
   -supper1            boolean    Make upper case
   -sformat1           string     Input sequence format
   -sdbname1           string     Database name
   -sid1               string     Entryname
   -ufo1               string     UFO features
   -fformat1           string     Features format
   -fopenfile1         string     Features file name

   "-outfile" associated qualifiers
   -odirectory2        string     Output directory

   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write standard output
   -filter             boolean    Read standard input, write standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages

Input file format

   pepstats reads a normal protein sequence USA.

  Input files for usage example

   'tsw:laci_ecoli' is a sequence entry in the example protein database
   'tsw'

  Database entry: tsw:laci_ecoli

ID   LACI_ECOLI              Reviewed;         360 AA.
AC   P03023; O09196; P71309; Q2MC79; Q47338;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2003, sequence version 3.
DT   20-MAR-2007, entry version 87.
DE   Lactose operon repressor.
GN   Name=lacI; OrderedLocusNames=b0345, JW0336;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=78246991; PubMed=355891; DOI=10.1038/274765a0;
RA   Farabaugh P.J.;
RT   "Sequence of the lacI gene.";
RL   Nature 274:765-769(1978).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Chen J., Matthews K.K.S.M.;
RL   Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Marsh S.;
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains


  [Part of this file has been deleted for brevity]

DR   Pfam; PF00532; Peripla_BP_1; 1.
DR   PRINTS; PR00036; HTHLACI.
DR   SMART; SM00354; HTH_LACI; 1.
DR   PROSITE; PS00356; HTH_LACI_1; 1.
DR   PROSITE; PS50932; HTH_LACI_2; 1.
KW   3D-structure; Complete proteome; Direct protein sequencing;
KW   DNA-binding; Repressor; Transcription; Transcription regulation.
FT   CHAIN         1    360       Lactose operon repressor.
FT                                /FTId=PRO_0000107963.
FT   DOMAIN        1     58       HTH lacI-type.
FT   DNA_BIND      6     25       H-T-H motif.
FT   VARIANT     282    282       Y -> D (in T41 mutant).
FT   MUTAGEN      17     17       Y->H: Broadening of specificity.
FT   MUTAGEN      22     22       R->N: Recognizes an operator variant.
FT   CONFLICT    286    286       L -> S (in Ref. 1, 4 and 7).
FT   STRAND       63     69
FT   HELIX        74     89
FT   STRAND       93     98
FT   STRAND      101    103
FT   HELIX       104    115
FT   TURN        116    118
FT   STRAND      122    126
FT   HELIX       130    139
FT   TURN        140    142
FT   STRAND      145    150
FT   STRAND      154    156
FT   STRAND      158    161
FT   HELIX       163    177
FT   STRAND      181    186
FT   HELIX       192    207
FT   STRAND      213    217
FT   HELIX       222    234
FT   STRAND      240    246
FT   HELIX       247    259
FT   TURN        265    267
FT   STRAND      268    271
FT   HELIX       277    281
FT   STRAND      282    284
FT   STRAND      287    290
FT   HELIX       293    308
FT   STRAND      314    319
FT   STRAND      322    324
FT   HELIX       354    356
SQ   SEQUENCE   360 AA;  38590 MW;  347A8DEE92D736CB CRC64;
     MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ
     SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS
     GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ
     QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT
     AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS
     VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ
//

Output file format

  Output files for usage example

  File: laci_ecoli.pepstats

PEPSTATS of LACI_ECOLI from 1 to 360

Molecular weight = 38590.05             Residues = 360
Average Residue Weight  = 107.195       Charge   = 1.5
Isoelectric Point = 6.8820
A280 Molar Extinction Coefficient  = 21620
A280 Extinction Coefficient 1mg/ml = 0.56
Improbability of expression in inclusion bodies = 0.660

Residue         Number          Mole%           DayhoffStat
A = Ala         44              12.222          1.421
B = Asx         0               0.000           0.000
C = Cys         3               0.833           0.287
D = Asp         17              4.722           0.859
E = Glu         15              4.167           0.694
F = Phe         4               1.111           0.309
G = Gly         22              6.111           0.728
H = His         7               1.944           0.972
I = Ile         18              5.000           1.111
J = ---         0               0.000           0.000
K = Lys         11              3.056           0.463
L = Leu         41              11.389          1.539
M = Met         10              2.778           1.634
N = Asn         12              3.333           0.775
O = ---         0               0.000           0.000
P = Pro         14              3.889           0.748
Q = Gln         28              7.778           1.994
R = Arg         19              5.278           1.077
S = Ser         32              8.889           1.270
T = Thr         19              5.278           0.865
U = ---         0               0.000           0.000
V = Val         34              9.444           1.431
W = Trp         2               0.556           0.427
X = Xaa         0               0.000           0.000
Y = Tyr         8               2.222           0.654
Z = Glx         0               0.000           0.000

Property        Residues                Number          Mole%
Tiny            (A+C+G+S+T)             120             33.333
Small           (A+B+C+D+G+N+P+S+T+V)   197             54.722
Aliphatic       (A+I+L+V)               137             38.056
Aromatic        (F+H+W+Y)               21               5.833
Non-polar       (A+C+F+G+I+L+M+P+V+W+Y) 200             55.556
Polar           (D+E+H+K+N+Q+R+S+T+Z)   160             44.444
Charged         (B+D+E+H+K+R+Z)         69              19.167
Basic           (H+K+R)                 37              10.278
Acidic          (B+D+E+Z)               32               8.889

Data files

   The Dayhoff statistic is read from the EMBOSS data file
   'Edayhoff.freq'. You can inspect and modify this file by copying it
   into your current directory with the command: 'embossdata -fetch'.

   EMBOSS data files are distributed with the application and stored in
   the standard EMBOSS data directory, which is defined by the EMBOSS
   environment variable EMBOSS_DATA.

   To see the available EMBOSS data files, run:

% embossdata -showall

   To fetch one of the data files (for example 'Exxx.dat') into your
   current directory for you to inspect or modify, run:

% embossdata -fetch -file Exxx.dat

   Users can provide their own data files in their own directories.
   Project specific files can be put in the current directory, or for
   tidier directory listings in a subdirectory called ".embossdata".
   Files for all EMBOSS runs can be put in the user's home directory, or
   again in a subdirectory called ".embossdata".

   The directories are searched in the following order:
     * . (your current directory)
     * .embossdata (under your current directory)
     * ~/ (your home directory)
     * ~/.embossdata

Notes

   None.

References

    1. Roger G. Harrison "Expression of soluble heterologous proteins via
       fusion with NusA protein" in inNovations 11, June 2000, p 4 - 7.

Warnings

   None.

Diagnostic Error Messages

   None.

Exit status

   It always exits with a status of 0.

Known bugs

   None.

See also

   Program name                         Description
   backtranambig Back translate a protein sequence to ambiguous codons
   backtranseq   Back translate a protein sequence
   charge        Protein charge plot
   checktrans    Reports STOP codons and ORF statistics of a protein
   compseq       Count composition of dimer/trimer/etc words in a sequence
   emowse        Protein identification by mass spectrometry
   freak         Residue/base frequency table or plot
   iep           Calculates the isoelectric point of a protein
   mwcontam      Shows molwts that match across a set of files
   mwfilter      Filter noisy molwts from mass spec output
   octanol       Displays protein hydropathy
   pepinfo       Plots simple amino acid properties in parallel
   pepwindow     Displays protein hydropathy
   pepwindowall  Displays protein hydropathy of a set of sequences

Author(s)

   Alan Bleasby (ajb © ebi.ac.uk)
   European Bioinformatics Institute, Wellcome Trust Genome Campus,
   Hinxton, Cambridge CB10 1SD, UK

History

   Written (1999) - Alan Bleasby

Target users

   This program is intended to be used by everyone and everything, from
   naive users to embedded scripts.

Comments

   None
