#!/usr/bin/perl -w

#
# Copyright 2011, Ben Langmead <langmea@cs.jhu.edu>
#
# This file is part of Bowtie 2.
#
# Bowtie 2 is free software: you can redistribute it and/or modify
# it under the terms of the GNU General Public License as published by
# the Free Software Foundation, either version 3 of the License, or
# (at your option) any later version.
#
# Bowtie 2 is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
# GNU General Public License for more details.
#
# You should have received a copy of the GNU General Public License
# along with Bowtie 2.  If not, see <http://www.gnu.org/licenses/>.
#

##
# Give simple tests with known results to bowtie2.
#

use strict;
use warnings;
use Getopt::Long;
use FindBin qw($Bin);
use lib $Bin;
use List::Util qw(max min);
use Data::Dumper;
use DNA;
use Clone qw(clone);
use Test::Deep;
use File::Which qw(which);

my $bowtie2 = "";
my $bowtie2_build = "";
my $compressed;

GetOptions(
	"compressed-reads" => \$compressed,
	"bowtie2=s"        => \$bowtie2,
	"bowtie2-build=s"  => \$bowtie2_build) || die "Bad options";

if(! -x $bowtie2 || ! -x $bowtie2_build) {
	my $bowtie2_dir = `dirname $bowtie2`;
	my $bowtie2_exe = `basename $bowtie2`;
	my $bowtie2_build_exe = `basename $bowtie2_build`;
	chomp($bowtie2_dir);
	chomp($bowtie2_exe);
	chomp($bowtie2_build_exe);
	system("make -C $bowtie2_dir $bowtie2_exe $bowtie2_build_exe") && die;
}

(-x $bowtie2)       || die "Cannot run '$bowtie2'";
(-x $bowtie2_build) || die "Cannot run '$bowtie2_build'";

my $compiled_with_sra = (`$bowtie2 --version` =~ /USE_SRA/) && defined(which "latf-load");
my $should_test_bam = defined(which "samtools");

my @cases = (

	# File format cases

	# -F: FASTA continuous

	{ name   => "FASTA-continuous 1",
		ref    => [ "AGCATCGATCAGTATCTGA" ],
		#            0123456789012345678
		#            AGCATCGATC
		#                     CAGTATCTGA
		cont_fasta_reads => ">seq1\nAGCATCGATCAGTATCTGA\n",
		idx_map => { "seq1_0" => 0, "seq1_9" => 1 },
		args   => "-F 10,9",
		hits   => [{ 0 => 1 }, { 9 => 1 }] },

	{ name   => "FASTA-continuous 2",
		ref    => [ "AGCATCGATCAGTATCTGA" ],
		#            0123456789012345678
		#      seq1: AGCATCGATCAGTATCTG
		#            AGCATCGATC
		#      seq2: AGCATCGATCAGTATCTGA
		#            AGCATCGATC
		#                     CAGTATCTGA
		cont_fasta_reads => ">seq1\nAGCATCGATCAGTATCTG\n".
		                    ">seq2\nAGCATCGATCAGTATCTGA\n",
		idx_map => { "seq1_0" => 0, "seq2_0" => 1, "seq2_9" => 2 },
		args   => "-F 10,9",
		hits   => [{ 0 => 1 }, { 0 => 1 }, { 9 => 1 }] },

	{ name   => "FASTA-continuous 3",
		ref    => [ "AGCATCGATCAGTATCTGA" ],
		#            0123456789012345678
		#            AGCATCGATC
		#                     CAGTATCTGA
		cont_fasta_reads => ">seq1\nAGCATCGATCAGTATCTGA\n",
		idx_map => { "seq1_0" => 0 },
		args   => "-F 10,9 -u 1",
		hits   => [{ 0 => 1 }] },

	{ name   => "FASTA-continuous 4",
		ref    => [ "AGCATCGATCAGTATCTGA" ],
		#            0123456789012345678
		#            AGCATCGATC
		#                     CAGTATCTGA
		cont_fasta_reads => ">seq1\nAGCATCGATCAGTATCTGA\n",
		idx_map => { "seq1_9" => 0 },
		args   => "-F 10,9 -s 1",
		hits   => [{ 9 => 1 }] },

	{ name   => "FASTA-continuous 5",
		ref    => [ "AGCATCGATCAGTATCTGA" ],
		#            0123456789012345678
		#      seq1: AGCATCGATCAGTATCTG
		#            AGCATCGATC
		#      seq2: AGCATCGATCAGTATCTGA
		#            AGCATCGATC
		#                     CAGTATCTGA
		cont_fasta_reads => ">seq1\nAGCATCGATCAGTATCTG\n".
		                    ">seq2\nAGCATCGATCAGTATCTGA\n",
		idx_map => { "seq2_0" => 0 },
		args   => "-F 10,9 -u 1 -s 1",
		hits   => [{ 0 => 1 }] },

	{ name   => "FASTA-continuous 6",
		ref    => [ "AGCATCGATCAG" ],
		#            012345678901
		#      seq1: AGCATCGATC
		#             GCATCGATCA
		#              CATCGATCAG
		cont_fasta_reads => ">seq1\nAGCATCGATCAG\n",
		idx_map => { "seq1_0" => 0, "seq1_1" => 1, "seq1_2" => 2 },
		args   => "-F 10,1",
		hits   => [{ 0 => 1 }, { 1 => 1 }, { 2 => 1 }] },

	# -c

	{ name   => "Align should not fail when first seq in ref is all Ns",
		ref    => [ "NNNNNNNNNNNNNNN", "AGCATCGATCAGTATCTGA" ],
		cline_reads => "CATCGATCAGTATCTG",
		hits   => [{ 2 => 1 }] },

	{ name   => "Align should not fail when multiple seqs are all Ns",
		ref    => [ "NNNNNNNNNNNNNNN", "NNNNNNNNNNNNNNN", "AGCATCGATCAGTATCTGA" ],
		cline_reads => "CATCGATCAGTATCTG",
		hits   => [{ 2 => 1 }] },

	{ name   => "Should account for stretch of Ns at beginning",
		ref    => [ "NNNNNNNNNNNNNNNAGCATCGATCAGTATCTGA" ],
		cline_reads => "CATCGATCAGTATCTG",
		hits   => [{ 17 => 1 }] },

	{ name   => "Cline 1",
		ref    => ["AGCATCGATCAGTATCTGA" ],
		cline_reads => "CATCGATCAGTATCTG",
		hits   => [{ 2 => 1 }] },

	{ name   => "Cline 2",
		ref    => [ "AGCATCGATCAGTATCTGA" ],
		cline_reads => "CATCGATCAGTATCTG:IIIIIIIIIIIIIIII",
		hits   => [{ 2 => 1 }] },

	{ name   => "Cline 2",
		ref    => [ "AGCATCGATCAGTATCTGA" ],
		cline_reads => "CATCGATCAGTATCTG:ABCDEDGHIJKLMNOP",
		hits   => [{ 2 => 1 }] },

	{ name   => "Cline 4",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  cline_reads  => "CATCGATCAGTATCTG:ABCDEDGHIJKLMNO", # qual too short
	  should_abort => 1},

	{ name   => "Cline 5",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  cline_reads  => "CATCGATCAGTATCTG:ABCDEDGHIJKLMNOPQ", # qual too long
	  should_abort => 1},

	{ name   => "trim-to: trim from 5'-end",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  cline_reads => "CATCGATCAGTATCTG:IIIIIIIIIIIIIIII\n",
	  args   => "--trim-to 5:12",
	  norc   => 1,
	  hits   => [{ 6 => 1 }] },

	{ name   => "trim-to: trim from 3'-end",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  cline_reads => "CATCGATCAGTATCTG:IIIIIIIIIIIIIIII\n",
	  args   => "--trim-to 3:12",
	  norc   => 1,
	  hits   => [{ 2 => 1 }] },

	{ name   => "trim-to: trim from 3'-end (default)",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  cline_reads => "CATCGATCAGTATCTG:IIIIIIIIIIIIIIII\n",
	  args   => "--trim-to 12",
	  norc   => 1,
	  hits   => [{ 2 => 1 }] },

	{ name   => "trim-to: invalid position",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  cline_reads => "CATCGATCAGTATCTG:IIIIIIIIIIIIIIII\n",
	  args   => "--trim-to 4:12",
	  norc   => 1,
	  should_abort   => 1 },

	{ name   => "trim-to: invalid count",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  cline_reads => "CATCGATCAGTATCTG:IIIIIIIIIIIIIIII\n",
	  args   => "--trim-to 5:-12",
	  norc   => 1,
	  should_abort   => 1 },

	# Part of sequence is trimmed
	{ name   => "Cline 7",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  cline_reads => "CATCGATCAGTATCTG:IIIIIIIIIIIIIIII\n",
	  args   => "--trim3 4",
	  norc   => 1,
	  hits   => [{ 2 => 1 }] },

	# Whole sequence is trimmed
	{ name   => "Cline 8",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  cline_reads => "CATCGATCAGTATCTG:IIIIIIIIIIIIIIII",
	  args   => "--trim5 16",
	  hits   => [{ "*" => 1 }] },

	# Sequence is skipped
	{ name   => "Cline 9",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  cline_reads => "CATCGATCAGTATCTG:IIIIIIIIIIIIIIII",
	  args   => "-s 1",
	  hits   => [{ }] },

	{ name   => "Cline multiread 1",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  cline_reads => "CATCGATCAGTATCTG:IIIIIIIIIIIIIIII,".
	                 "ATCGATCAGTATCTG:IIIIIIIIIIIIIII\n\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name   => "Cline multiread 2",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
		args   =>   "-u 1",
	  cline_reads => "CATCGATCAGTATCTG:IIIIIIIIIIIIIIII,".
	                 "ATCGATCAGTATCTG:IIIIIIIIIIIIIII\n\n",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Cline multiread 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 2",
	  cline_reads  => "CATCGATCAGTATCTG,".
	                  "ATCGATCAGTATCTG\r\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	# Paired-end reads that should align
	{ name     => "Cline paired 1",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  cline_reads1  => "AGCATCGATC:IIIIIIIIII,".
	                   "TCAGTTTTTGA",
	  cline_reads2  => "TCAGTTTTTGA,".
	                   "AGCATCGATC:IIIIIIIIII",
	  pairhits => [ { "0,8" => 1 }, { "0,8" => 1 } ] },

	# Paired-end reads that should align
	{ name     => "Cline paired 2",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-s 1",
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  cline_reads1  => "AGCATCGATC:IIIIIIIIII,".
	                   "TCAGTTTTTGA:IIIIIIIIIII",
	  cline_reads2  => "TCAGTTTTTGA:IIIIIIIIIII,".
	                   "AGCATCGATC:IIIIIIIIII",
	  pairhits => [ { }, { "0,8" => 1 } ] },

	# Paired-end reads that should align
	{ name     => "Cline paired 3",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-u 1",
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  cline_reads1  => "AGCATCGATC:IIIIIIIIII,".
	                   "TCAGTTTTTGA:IIIIIIIIIII",
	  cline_reads2  => "TCAGTTTTTGA:IIIIIIIIIII,".
	                   "AGCATCGATC:IIIIIIIIII",
	  pairhits => [ { "0,8" => 1 }, { } ] },

	# Paired-end reads with left end entirely trimmed away
	{ name     => "Cline paired 4",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-3 7",
	  #                  AGCATCG
	  #                        GATCAAAAACTGA
	  #                  0123456789012345678
	  cline_reads1  => "AGCATCG:IIIIIII",
	  cline_reads2  => "GATCAAAAACTGA:IIIIIIIIIIIII",
		#                               GATCAGTTTTTGA
	  pairhits => [ { "*,6" => 1 } ] },

	# -q

	{ name   => "Fastq 1",
	  ref    => [   "AGCATCGATCAGTATCTGA" ],
	  fastq  => "\@r0\nCATCGATCAGTATCTG\n+\nIIIIIIIIIIIIIIII",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Fastq 2",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fastq  => "\@r0\nCATCGATCAGTATCTG\n+\nIIIIIIIIIIIIIIII\n", # extra newline
	  hits   => [{ 2 => 1 }] },

	{ name   => "Fastq 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fastq  => "\@r0\nCATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIIII\n",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Fastq 4",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fastq  => "\@r0\nCATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIII\n", # qual too short
		#                                       CATCGATCAGTATCTG
	  should_abort => 1},

	# Name line doesn't start with @
	{ name   => "Fastq 5",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fastq  => "\@r0\nCATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIII\n",
	  should_abort => 1,
	  hits   => [{ }] },

	# Name line doesn't start with @ (2)
	{ name   => "Fastq 6",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fastq  => "r0\nCATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIII\n",
	  should_abort => 1,
	  hits   => [{ }] },

	# Part of sequence is trimmed
	{ name   => "Fastq 7",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fastq  => "\@r0\nCATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIIII\n",
	  args   => "--trim3 4",
	  norc   => 1,
	  hits   => [{ 2 => 1 }] },

	# Whole sequence is trimmed
	{ name   => "Fastq 8",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fastq  => "\@r0\nCATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIIII\n",
	  args   => "--trim5 16",
	  hits   => [{ "*" => 1 }] },

	# Sequence is skipped
	{ name   => "Fastq 9",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fastq  => "\@r0\nCATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIIII\n",
	  args   => "-s 1",
	  hits   => [{ }] },

	# Like Fastq 1 but with many extra newlines
	{ name   => "Fastq multiread 1",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fastq  => "\@r0\nCATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIIII\n".
	            "\@r1\nATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIII\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	# Like Fastq multiread 1 but with -u 1
	{ name   => "Fastq multiread 2",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 1",
	  fastq  => "\@r0\nCATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIIII\n".
	            "\@r1\nATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIII\n",
	  hits   => [{ 2 => 1 }] },

	# Like Fastq multiread 1 but with -u 2
	{ name   => "Fastq multiread 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 2",
	  fastq  => "\@r0\nCATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIIII\n".
	            "\@r1\nATCGATCAGTATCTG\r\n+\nIIIIIIIIIIIIIII\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	# Paired-end reads that should align
	{ name     => "Fastq paired 1",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  fastq1  => "\@r0\nAGCATCGATC\r\n+\nIIIIIIIIII\n".
	             "\@r1\nTCAGTTTTTGA\r\n+\nIIIIIIIIIII\n",
	  fastq2  => "\@r0\nTCAGTTTTTGA\n+\nIIIIIIIIIII\n".
	             "\@r1\nAGCATCGATC\r\n+\nIIIIIIIIII\n",
	  pairhits => [ { "0,8" => 1 }, { "0,8" => 1 } ] },

	# Paired-end reads that should align
	{ name     => "Fastq paired 2",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-s 1",
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  fastq1  => "\@r0\nAGCATCGATC\r\n+\nIIIIIIIIII\n".
	             "\@r1\nTCAGTTTTTGA\n+\nIIIIIIIIIII\n",
	  fastq2  => "\@r0\nTCAGTTTTTGA\n+\nIIIIIIIIIII\n".
	             "\@r1\nAGCATCGATC\r\n+\nIIIIIIIIII\n",
	  pairhits => [ { }, { "0,8" => 1 } ] },

	# Paired-end reads that should align
	{ name     => "Fastq paired 3",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-u 1",
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  fastq1  => "\@r0\nAGCATCGATC\r\n+\nIIIIIIIIII\n".
				 "\@r1\nTCAGTTTTTGA\r\n+\nIIIIIIIIIII\n",
	  fastq2  => "\@r0\nTCAGTTTTTGA\n+\nIIIIIIIIIII\n".
				 "\@r1\nAGCATCGATC\r\n+\nIIIIIIIIII\n",
	  pairhits => [ { "0,8" => 1 }, { } ] },

	# Paired-end reads with left end entirely trimmed away
	{ name     => "Fastq paired 4",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-3 7",
	  #                  AGCATCG
	  #                        GATCAAAAACTGA
	  #                  0123456789012345678
	  fastq1  => "\@r0\nAGCATCG\n+\nIIIIIII\n",
	  fastq2  => "\@r0\nGATCAAAAACTGA\n+\nIIIIIIIIIIIII\n",
		#                               GATCAGTTTTTGA
	  pairhits => [ { "*,6" => 1 } ] },

	# -f

	{ name   => "Fasta 1",
	  ref    => [  "AGCATCGATCAGTATCTGA" ],
	  fasta  => ">r0\nCATCGATCAGTATCTG",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Fasta 2",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fasta  => ">r0\nCATCGATCAGTATCTG\n",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Fasta 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fasta  => "\n\n\r\n>r0\nCATCGATCAGTATCTG\r\n\n",
	  hits   => [{ 2 => 1 }] },

	# Name line doesn't start with >
	{ name   => "Fasta 5",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fasta  => "\n\n\r\nr0\nCATCGATCAGTATCTG\r",
	  should_abort => 1,
	  hits   => [{ }] },

	# Name line doesn't start with > (2)
	{ name   => "Fasta 6",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fasta  => "r0\nCATCGATCAGTATCTG\r",
	  should_abort => 1,
	  hits   => [{ }] },

	{ name   => "Fasta 7",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fasta  => "\n\n\r\n\>r0\nCATCGATCAGTATCTG\r\n",
	  args   => "--trim3 4",
	  norc   => 1,
	  hits   => [{ 2 => 1 }] },

	{ name   => "Fasta 8",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fasta  => "\n\n\r\n\>r0\nCATCGATCAGTATCTG\r\n",
	  args   => "--trim3 16",
	  hits   => [{ "*" => 1 }] },

	{ name   => "Fasta 9",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fasta  => "\n\n\r\n>r0\nCATCGATCAGTATCTG\r\n",
	  args   => "-s 1",
	  hits   => [{ }] },

	{ name   => "Fasta multiread 1",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  fasta  => "\n\n\r\n>r0\nCATCGATCAGTATCTG\n\n".
	            "\n\n\r\n>r1\nATCGATCAGTATCTG\n\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name   => "Fasta multiread 2",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 1",
	  fasta  => "\n\n\r\n>r0\nCATCGATCAGTATCTG\r\n".
	            "\n\n\r\n>r1\nATCGATCAGTATCTG\r\n",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Fasta multiread 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 2",
	  fasta  => "\n\n\r\n>r0\nCATCGATCAGTATCTG\r\n".
	            "\n\n\r\n>r1\nATCGATCAGTATCTG\r\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name   => "Fasta multiline 1",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 2",
	  fasta  => "\n\n\r\n>r0\nCATCGAT\nCAGTATCTG\r\n".
	            "\n\n\r\n>r1\nATCGATCAGTATCTG\r\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name   => "Fasta multiline 2",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 2",
	  fasta  => "\n\n\r\n>r0\nCATCGAT\nCAGTATCTG\r\n".
	            "\n\n\r\n>r1\nATCGATC\nAGTATCTG\r\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name   => "Fasta multiline 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 2",
	  fasta  => "\n\n\r\n>r0\nCATCGAT\nCAGTATCTG\r\n".
	            "\n\n\r\n>\nATCGATC\nAGTATCTG\r\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name     => "Fasta paired 1",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  fasta1  => "\n\n\r\n>r0\nAGCATCGATC\r\n".
	             "\n\n>r1\nTCAGTTTTTGA\r\n",
	  fasta2  => "\n\n\r\n>r0\nTCAGTTTTTGA\n".
	             "\n\n\r\n>r1\nAGCATCGATC",
	  pairhits => [ { "0,8" => 1 }, { "0,8" => 1 } ] },

	{ name     => "Fasta paired 2",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-s 1",
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  fasta1  => ">r0\nAGCATCGATC\r\n".
	             "\n\n>r1\nTCAGTTTTTGA\n",
	  fasta2  => "\n\n\r\n>r0\nTCAGTTTTTGA\n".
	             "\n\n\r\n>r1\nAGCATCGATC",
	  pairhits => [ { }, { "0,8" => 1 } ] },

	{ name     => "Fasta paired 3",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-u 1",
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  fasta1  => "\n\n\r\n>r0\nAGCATCGATC\r\n".
	             "\n\n>r1\nTCAGTTTTTGA\r\n",
	  fasta2  => "\n\n\r\n>r0\nTCAGTTTTTGA\n".
	             "\n\n\r\n>r1\nAGCATCGATC",
	  pairhits => [ { "0,8" => 1 }, { } ] },

	# Paired-end reads with left end entirely trimmed away
	{ name     => "Fasta paired 4",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-3 7",
	  #                  AGCATCG
	  #                        GATCAAAAACTGA
	  #                  0123456789012345678
	  fasta1  => ">\nAGCATCG\n",
	  fasta2  => ">\nGATCAAAAACTGA\n",
		#                               GATCAGTTTTTGA
	  pairhits => [ { "*,6" => 1 } ] },

	# -r

	{ name   => "Raw 1",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  raw    =>     "CATCGATCAGTATCTG",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Raw 2",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  raw    => "CATCGATCAGTATCTG\n",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Raw 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  raw    => "\n\n\nCATCGATCAGTATCTG\n\n",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Raw 7",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  raw    => "\n\n\r\nCATCGATCAGTATCTG\r\n",
	  args   => "--trim3 4",
	  norc   => 1,
	  hits   => [{ 2 => 1 }] },

	{ name   => "Raw 8",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  raw    => "\n\n\r\nCATCGATCAGTATCTG\r\n",
	  args   => "--trim3 16",
	  hits   => [{ "*" => 1 }] },

	{ name   => "Raw 9",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  raw    => "CATCGATCAGTATCTG\n",
	  args   => "-s 1",
	  hits   => [{ }] },

	{ name   => "Raw multiread 1",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  raw    => "\n\n\r\nCATCGATCAGTATCTG\n\n".
	            "\n\n\r\nATCGATCAGTATCTG\n\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name   => "Raw multiread 2",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 1",
	  raw    => "\n\n\r\nCATCGATCAGTATCTG\r\n".
	            "\n\n\r\nATCGATCAGTATCTG\r\n",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Raw multiread 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 2",
	  raw    => "\n\n\r\nCATCGATCAGTATCTG\r\n".
	            "\n\n\r\nATCGATCAGTATCTG\r\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name     => "Raw paired 1",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  raw1    => "\n\n\r\nAGCATCGATC\r\n".
	             "\n\nTCAGTTTTTGA\r\n",
	  raw2    => "\n\n\r\nTCAGTTTTTGA\n".
	             "\n\n\r\nAGCATCGATC",
	  pairhits => [ { "0,8" => 1 }, { "0,8" => 1 } ] },

	{ name     => "Raw paired 2",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-s 1",
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  raw1    => "AGCATCGATC\r\n".
	             "\n\nTCAGTTTTTGA\n",
	  raw2    => "\n\n\r\nTCAGTTTTTGA\n".
	             "\n\n\r\nAGCATCGATC",
	  pairhits => [ { }, { "0,8" => 1 } ] },

	{ name     => "Raw paired 3",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-u 1",
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  raw1    => "\n\n\r\nAGCATCGATC\r\n".
	             "\n\nTCAGTTTTTGA\r\n",
	  raw2    => "\n\n\r\nTCAGTTTTTGA\n".
	             "\n\n\r\nAGCATCGATC",
	  pairhits => [ { "0,8" => 1 }, { } ] },

	# Paired-end reads with left end entirely trimmed away
	{ name     => "Raw paired 4",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-3 7",
	  #                  AGCATCG
	  #                        GATCAAAAACTGA
	  #                  0123456789012345678
	  raw1     => "\nAGCATCG\n",
	  raw2     => "\nGATCAAAAACTGA\n",
		#                               GATCAGTTTTTGA
	  pairhits => [ { "*,6" => 1 } ] },

	# --12 / --tab5 / --tab6

	{ name   => "Tabbed 1",
	  ref    => [   "AGCATCGATCAGTATCTGA" ],
	  tabbed => "r0\tCATCGATCAGTATCTG\tIIIIIIIIIIIIIIII",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Tabbed 2",
	  ref    => [   "AGCATCGATCAGTATCTGA" ],
	  tabbed => "r0\tCATCGATCAGTATCTG\tIIIIIIIIIIIIIIII\n",  # extra newline
	  hits   => [{ 2 => 1 }] },

	{ name   => "Tabbed 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  tabbed => "\n\n\r\nr0\tCATCGATCAGTATCTG\tIIIIIIIIIIIIIIII\n\n",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Tabbed 4",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  tabbed => "\n\n\r\nr0\tCATCGATCAGTATCTG\tIIIIIIIIIIIIIII\n\n", # qual too short
	  should_abort => 1},

	{ name   => "Tabbed 5",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  tabbed => "\n\n\r\nr0\tCATCGATCAGTATCTG\tIIIIIIIIIIIIIIIII\n\n", # qual too long
	  should_abort => 1},

	{ name   => "Tabbed 7",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  tabbed => "\n\n\r\nr0\tCATCGATCAGTATCTG\tIIIIIIIIIIIIIIII\n\n",
	  args   => "--trim3 4",
	  norc   => 1,
	  hits   => [{ 2 => 1 }] },

	{ name   => "Tabbed 8",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  tabbed => "\n\n\r\nr0\tCATCGATCAGTATCTG\tIIIIIIIIIIIIIIII\n\n",
	  args   => "--trim5 16",
	  hits   => [{ "*" => 1 }] },

	{ name   => "Tabbed 9",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  tabbed => "\n\n\r\nr0\tCATCGATCAGTATCTG\tIIIIIIIIIIIIIIII\n\n",
	  args   => "-s 1",
	  hits   => [{ }] },

	{ name   => "Tabbed multiread 1",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  tabbed => "\n\n\r\nr0\tCATCGATCAGTATCTG\tIIIIIIIIIIIIIIII\n\n".
	            "\n\n\r\nr1\tATCGATCAGTATCTG\tIIIIIIIIIIIIIII\n\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name   => "Tabbed multiread 2",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 1",
	  tabbed => "\n\n\r\nr0\tCATCGATCAGTATCTG\tIIIIIIIIIIIIIIII\n\n".
	            "\n\n\r\nr1\tATCGATCAGTATCTG\tIIIIIIIIIIIIIII\n\n",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Tabbed multiread 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 2",
	  tabbed => "\n\n\r\nr0\tCATCGATCAGTATCTG\tIIIIIIIIIIIIIIII\n\n".
	            "\n\n\r\nr1\tATCGATCAGTATCTG\tIIIIIIIIIIIIIII\n\n",
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name     => "Tabbed paired 1",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  tabbed  => "\n\n\r\nr0\tAGCATCGATC\tIIIIIIIIII\tTCAGTTTTTGA\tIIIIIIIIIII\n\n".
	             "\n\nr1\tTCAGTTTTTGA\tIIIIIIIIIII\tAGCATCGATC\tIIIIIIIIII\n\n",
	  paired => 1,
	  pairhits => [ { "0,8" => 1 }, { "0,8" => 1 } ] },

	{ name     => "Tabbed paired 2",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-s 1",
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  tabbed   => "r0\tAGCATCGATC\tIIIIIIIIII\tTCAGTTTTTGA\tIIIIIIIIIII\n\n".
	             "\nr1\tTCAGTTTTTGA\tIIIIIIIIIII\tAGCATCGATC\tIIIIIIIIII",
	  paired   => 1,
	  pairhits => [ { }, { "0,8" => 1 } ] },

	{ name     => "Tabbed paired 3",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-u 1",
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  tabbed   => "\n\n\r\nr0\tAGCATCGATC\tIIIIIIIIII\tTCAGTTTTTGA\tIIIIIIIIIII\n\n".
	              "\n\nr1\tTCAGTTTTTGA\tIIIIIIIIIII\tAGCATCGATC\tIIIIIIIIII",
	  paired   => 1,
	  pairhits => [ { "0,8" => 1 }, { } ] },

	# Paired-end reads with left end entirely trimmed away
	{ name     => "Tabbed paired 4",
	  ref      => [     "AGCATCGATCAAAAACTGA" ],
	  args     => "-3 7",
	  #                  AGCATCG
	  #                        GATCAAAAACTGA
	  #                  0123456789012345678
	  tabbed     => "\nr0\tAGCATCG\tIIIIIII\tGATCAAAAACTGA\tIIIIIIIIIIIII\n",
		paired   => 1,
	  pairhits => [ { "*,6" => 1 } ] },

	# --qseq

	{ name   => "Qseq 1",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  qseq   => join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "X", "Y",
						   "Index",
						   "0", # Mate
						   "CATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIIII",
						   "1"),
	  hits   => [{ 2 => 1 }] },

	{ name   => "Qseq 2",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  qseq   => join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "X", "Y",
						   "Index",
						   "0", # Mate
						   "CATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIIII",
						   "1")."\n",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Qseq 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  qseq   => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "X", "Y",
						   "Index",
						   "0", # Mate
						   "CATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIIII",
						   "1")."\n\n",
	  hits   => [{ 2 => 1 }] },

	{ name   => "Qseq 4",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  qseq   => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "X", "Y",
						   "Index",
						   "0", # Mate
						   "CATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIII",
						   "1")."\n\n",
	  should_abort => 1},

	{ name   => "Qseq 7",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  qseq   => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "X", "Y",
						   "Index",
						   "0", # Mate
						   "CATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIIII",
						   "1")."\n\n",
	  args   => "--trim3 4",
	  norc   => 1,
	  hits   => [{ 2 => 1 }] },

	{ name   => "Qseq 8",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  qseq   => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "X", "Y",
						   "Index",
						   "0", # Mate
						   "CATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIIII",
						   "1")."\n\n",
	  args   => "--trim3 16",
	  hits   => [{ "*" => 1 }] },

	{ name   => "Qseq 9",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  qseq   => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "X", "Y",
						   "Index",
						   "0", # Mate
						   "CATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIIII",
						   "1")."\n\n",
	  args   => "-s 1",
	  hits   => [{ }] },

	{ name   => "Qseq multiread 1",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  qseq   => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "10", "10",
						   "Index",
						   "0", # Mate
						   "CATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIIII",
						   "1")."\n\n".
						 join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "12", "15",
						   "Index",
						   "0", # Mate
						   "ATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIII",
						   "1")."\n\n",
	  idx_map => { "MachName_RunNum_Lane_Tile_10_10_Index" => 0,
	               "MachName_RunNum_Lane_Tile_12_15_Index" => 1 },
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name   => "Qseq multiread 2",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 1",
	  qseq   => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "10", "10",
						   "Index",
						   "0", # Mate
						   "CATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIIII",
						   "1")."\n\n".
						 join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "12", "15",
						   "Index",
						   "0", # Mate
						   "ATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIII",
						   "1")."\n\n",
	  idx_map => { "MachName_RunNum_Lane_Tile_10_10_Index" => 0,
	               "MachName_RunNum_Lane_Tile_12_15_Index" => 1 },
	  hits   => [{ 2 => 1 }] },

	{ name   => "Qseq multiread 3",
	  ref    => [ "AGCATCGATCAGTATCTGA" ],
	  args   =>   "-u 2",
	  qseq   => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "10", "10",
						   "Index",
						   "0", # Mate
						   "CATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIIII",
						   "1")."\n\n".
						 join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "12", "15",
						   "Index",
						   "0", # Mate
						   "ATCGATCAGTATCTG",
						   "IIIIIIIIIIIIIII",
						   "1")."\n\n",
	  idx_map => { "MachName_RunNum_Lane_Tile_10_10_Index" => 0,
	               "MachName_RunNum_Lane_Tile_12_15_Index" => 1 },
	  hits   => [{ 2 => 1 }, { 3 => 1 }] },

	{ name   => "Qseq paired 1",
	  ref    => [       "AGCATCGATCAAAAACTGA" ],
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  qseq1  => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "10", "10",
						   "Index",
						   "1", # Mate
						   "AGCATCGATC",
						   "ABCBGACBCB",
						   "1")."\n\n".
						 join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "12", "15",
						   "Index",
						   "1", # Mate
						   "TCAGTTTTTGA",
						   "95849456875",
						   "1")."\n\n",
	  qseq2  => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "10", "10",
						   "Index",
						   "2", # Mate
						   "TCAGTTTTTGA",
						   "ABCBGACBCBA",
						   "1")."\n\n".
						 join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "12", "15",
						   "Index",
						   "2", # Mate
						   "AGCATCGATC",
						   "AGGCBBGCBG",
						   "1")."\n\n",
	  idx_map => { "MachName_RunNum_Lane_Tile_10_10_Index" => 0,
	               "MachName_RunNum_Lane_Tile_12_15_Index" => 1 },
	  pairhits => [ { "0,8" => 1 }, { "0,8" => 1 } ] },

	{ name   => "Qseq paired 2",
	  ref    => [       "AGCATCGATCAAAAACTGA" ],
	  #                  AGCATCGATC
	  #                          TCAAAAACTGA
	  #                  0123456789012345678
	  args     => "-s 1",
	  qseq1  => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "10", "10",
						   "Index",
						   "1", # Mate
						   "AGCATCGATC",
						   "ABCBGACBCB",
						   "1")."\n\n".
						 join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "12", "15",
						   "Index",
						   "1", # Mate
						   "TCAGTTTTTGA",
						   "95849456875",
						   "1")."\n\n",
	  qseq2  => "\n\n\n".join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "10", "10",
						   "Index",
						   "2", # Mate
						   "TCAGTTTTTGA",
						   "ABCBGACBCBA",
						   "1")."\n\n".
						 join("\t", "MachName",
	                       "RunNum",
						   "Lane",
						   "Tile",
						   "12", "15",
						   "Index",
						   "2", # Mate
						   "AGCATCGATC",
						   "AGGCBBGCBG",
						   "1")."\n\n",
	  idx_map => { "MachName_RunNum_Lane_Tile_10_10_Index" => 0,
	               "MachName_RunNum_Lane_Tile_12_15_Index" => 1 },
	  pairhits => [ { }, { "0,8" => 1 } ] },

	{ name   => "Left-align insertion",
	  ref    => [ "GCGATATCTACGACTGCTACGTACAAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGATCTGACAGC" ],
	  norc   => 1,
	  reads => [                      "ACAAAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGA" ],
	  # ref:  AC-AAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGA
	  # read: ACAAAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGA
	  #          0123456789012345678901234567890123456789
	  cigar  => [ "2M1I40M" ],
	  samoptflags => [ {
		"MD:Z:42" => 1,
		"YT:Z:UU" => 1,
		"NM:i:1" => 1,
		"XG:i:1" => 1,
		"XO:i:1" => 1,
		"AS:i:-8" => 1 } ],
	  report => "",
	  args   => ""
	},

	{ name   => "Left-align deletion",
	  ref    => [ "GCGATATCTACGACTGCTACGTACAAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGATCTGACAGC" ],
	  norc   => 1,
	  reads => [                    "ACGTACAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGA" ],
	  # ref:  ACGTACAAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGA
	  # read: ACGTAC-AAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGA
	  #              012345678901234567890123456789012345678
	  cigar  => [ "6M1D39M" ],
	  samoptflags => [ {
		"MD:Z:6^A39" => 1,
		"YT:Z:UU" => 1,
		"NM:i:1" => 1,
		"XG:i:1" => 1,
		"XO:i:1" => 1,
		"AS:i:-8" => 1 } ],
	  report => "",
	  args   => ""
	},

	{ name   => "Left-align insertion with mismatch at LHS",
	  ref    => [ "GCGATATCTACGACTGCTACGCCCAAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGATCTGACAGC" ],
	  norc   => 1,
	  reads => [       "TATCTACGACTGCTACGCCCTAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGATCTGAC" ],
	  # ref:  GCGATATCTACGACTGCTACGCCCAAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGATCTGACAGC
	  # read:     TATCTACGACTGCTACGCCC-TAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGATCTGAC
	  #           01234567890123456789-012345678901234567890123456789012345678901234
	  #           0         1          2         3         4         5         6
	  cigar  => [ "20M1D45M" ],
	  samoptflags => [ {
		"MD:Z:20^A0A44" => 1,
		"YT:Z:UU" => 1,
		"NM:i:2" => 1,
		"XG:i:1" => 1,
		"XO:i:1" => 1,
		"XM:i:1" => 1,
		"AS:i:-14" => 1 } ],
	  report => "",
	  args   => ""
	},

	# This won't necessarily pass because the original location of the deletion
	# might
	#{ name   => "Left-align deletion with mismatch at LHS",
	#  ref    => [ "GCGATATCTACGACTGCTACGCCCAAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGATCTGACAGC" ],
	#  norc   => 1,
	#  reads => [     "TATCTACGACTGCTACGCCAAAAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGATCTGAC" ],
	#  # ref:  GCGATATCTACGACTGCTACGCCC-AAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGATCTGACAGC
	#  # read:     TATCTACGACTGCTACGCCAAAAAAAAAAAAAAAAGTGTTTACGTTGCTAGACTCGATCGATCTGAC
	#  #           01234567890123456789-012345678901234567890123456789012345678901234
	#  #           0         1          0         1         2         3         4
	#  cigar  => [ "20M1I45M" ],
	#  samoptflags => [ {
	#	"MD:Z:19C45" => 1,
	#	"YT:Z:UU" => 1,
	#	"NM:i:2" => 1,
	#	"XG:i:1" => 1,
	#	"XO:i:1" => 1,
	#	"XM:i:1" => 1,
	#	"AS:i:-14" => 1 } ],
	#  report => "",
	#  args   => ""
	#},

	{ name   => "Flags for when mates align non-concordantly, with many alignments for one",
	#              012345678
	  ref    => [ "CAGCGGCTAGCTATCGATCGTCCGGCAGCTATCATTATGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGATAGATCGCTCGCCTGACCTATATCGCTCGCGATTACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGATCGAGGATAGATCGCTCGCCTGACCTATATCGCTCGCGATTACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGATCGAGGATAGATCGCTCGCCTGACCTATATCGCTCGCGATTACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGATCG" ],
	#              0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901
	#              0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9
	#              0                                                                                                   1                                                                                                   2                                                                                                   3                                                                                                   4                                                                                                   5                                                                                                   6                                                                                                   7                                                                                                   8                                                                                                   9                                                                                                   0                                                                                                   1
	#              0                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       1
	  norc => 1,
	  mate1s => [   "GCGGCTAGCTATCGATCGTCCGGCAGCTATCATTATGA" ],
	  mate2s => [                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      "ACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGA" ],
	  #                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 981                                                                                1064                                                                               1147
	  #                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 ACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGA                                         ACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGA                                         ACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGA
	  samflags_map => [{ 981 => (1 | 128), 1064 => (1 | 128), 1147 => (1 | 128), 2 => (1 | 64) }],
	  report => "",
	  args   => ""
	},

	{ name   => "Flags for when mates align non-concordantly, with many alignments for one",
	#              012345678
	  ref    => [ "CAGCGGCTAGCTATCGATCGTCCGGCAGCTATCATTATGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGATAGATCGCTCGCCTGACCTATATCGCTCGCGATTACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGATCGAG" ],
	#              01234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567
	#              0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2
	#              0                                                                                                   1                                                                                                   2                                                                                                   3                                                                                                   4                                                                                                   5                                                                                                   6                                                                                                   7                                                                                                   8                                                                                                   9                                                                                                   0                                                                                                   1
	#              0                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       1
	  norc => 1,
	  mate1s => [   "GCGGCTAGCTATCGATCGTCCGGCAGCTATCATTATGA" ],
	  mate2s => [                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      "ACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGA" ],
	  tlen_map     => [{ 2 => 1021, 981 => -1021 }],
	  samflags_map => [{ 981 => (1 | 128), 2 => (1 | 64) }],
	  report => "",
	  args   => ""
	},

	{ name   => "MREVERSE flag is set if mate does not align",
	  ref    => [ "TTCACCATTTTTCTTTTCGTTAACTTGCCGTCAGCCTTTTCTTTGACCTCTTCTTTCTGTTCATGTGTATTTGCTGTCTCTTAGCCCAGACTTCCCGTGTCCTTTCCACCGGGCCTTTGAGAGGTCACAGGGTCTTGATGCTGTGGTCTTCATCTGCAGGTGTCTGACTTCCAGCAACTGCTGGCCTGTGCCAGGGTGCAAGCTGAGCACTGGAGTGGAGTTTTCCTGTGGAGAGGAGCCATGCCTAGAG" ],
	  norc   => 1,
	  mate1s => [   "AAGACCACAGCATCAAGACCCTGTGACCTCTCAAAGGCCCGGTGGAAAGGACACGGGAAGTCTGGGCTAAGAGACAGCAAATACACATGAACAGAAAGAA" ],
	  mate2s => [   "CAGCGGCCCTGCCTCCTACCCTTGCGCCTCATGACCAGCTTGTTGAAGAGATCCGACATCAAGTGCCCACCTTGGCTCGTGGCTCTCACTGCAACGGGAA" ],
	  samflags_map => [{ 50 => (1 | 8 | 16 | 64), "*" => (1 | 4 | 32 | 128) }],
	  report => "",
	  args   => ""
	},

	{ name   => "Flags for when mates align non-concordantly, with many alignments for one",
	#              012345678
	  ref    => [ "CAGCGGCTAGCTATCGATCGTCCGGCAGCTATCATTATGATAGGATAGATCGCTCGCCTGACCTATATCGCTCGCGATTACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGATCGAGGATAGATCGCTCGCCTGACCTATATCGCTCGCGATTACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGATCGAGGATAGATCGCTCGCCTGACCTATATCGCTCGCGATTACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGATCG" ],
	#              01234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789
	#              0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8
	#              0                                                                              *                    1                                                             *                                     2                                            *
	#              0                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       1
	  norc => 1,
	  mate1s => [   "GCGGCTAGCTATCGATCGTCCGGCAGCTATCATTATGA" ],
	  mate2s => [   "TCGTCGTGATGCGTCAGCTCGGATAGCCAGTACGTAGCTCGT" ],
	  #                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 981                                                                                1064                                                                               1147
	  #                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 ACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGA                                         ACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGA                                         ACGAGCTACGTACTGGCTATCCGAGCTGACGCATCACGACGA
	  samflags_map => [{ 79 => (1 | 2 | 16 | 128), 162 => (1 | 2 | 16 | 128), 245 => (1 | 2 | 16 | 128), 2 => (1 | 2 | 32 | 64) }],
	  report => "",
	  args   => ""
	},

	# Checking MD:Z strings for alignment
	{ name   => "MD:Z 1",
	  ref    => [ "CACGATCGACTTGA"."C"."TCATCGACGCTATCATTAATATATATAAGCCCGCATCTA" ],
	  reads  => [ "CACGATCGACTTGG".    "TCATCGACGCTATCATTAATATATATAAGCCCGCATCTA" ],
	  hits   => [ { 0 => 1 } ],
	  samoptflags => [ {
		"AS:i:-14"      => 1, # alignment score
		"XM:i:1"        => 1, # num mismatches
		"XO:i:1"        => 1, # num gap opens
		"XG:i:1"        => 1, # num gap extensions
		"NM:i:2"        => 1, # num edits
		"MD:Z:13^A0C39" => 1, # mismatching positions/bases
		"YT:Z:UU"       => 1, # type of alignment (concordant/discordant/etc)
	} ] },
	{ name   => "MD:Z 2",
	  ref    => [ "CACGATCGACTTGA"."A"."TCATCGACGCTATCATTAATATATATAAGCCCGCATCTA" ],
	  reads  => [ "CACGATCGACTTGG".    "TCATCGACGCTATCATTAATATATATAAGCCCGCATCTA" ],
	  #            0123456789012        012345678901234567890123456789012345678
	  hits   => [ { 0 => 1 } ],
	  samoptflags => [ {
		"AS:i:-14"      => 1, # alignment score
		"XM:i:1"        => 1, # num mismatches
		"XO:i:1"        => 1, # num gap opens
		"XG:i:1"        => 1, # num gap extensions
		"NM:i:2"        => 1, # num edits
		"MD:Z:13^A0A39" => 1, # mismatching positions/bases
		"YT:Z:UU"       => 1, # type of alignment (concordant/discordant/etc)
	} ] },
	{ name   => "MD:Z 3",
	  ref    => [ "CACGATCGACTTGT"."AA"."TCATCGACGCTATCATTAATATATATAAGCCCGCATCTA" ],
	  reads  => [ "CACGATCGACTTGC".     "TCATCGACGCTATCATTAATATATATAAGCCCGCATCTA" ],
	  #            0123456789012        012345678901234567890123456789012345678
	  hits   => [ { 0 => 1 } ],
	  samoptflags => [ {
		"AS:i:-17"       => 1, # alignment score
		"XM:i:1"         => 1, # num mismatches
		"XO:i:1"         => 1, # num gap opens
		"XG:i:2"         => 1, # num gap extensions
		"NM:i:3"         => 1, # num edits
		"MD:Z:13^TA0A39" => 1, # mismatching positions/bases
		"YT:Z:UU"        => 1, # type of alignment (concordant/discordant/etc)
	} ] },
	{ name   => "MD:Z 4",
	  ref    => [ "CACGATCGACTTGN"."NN"."TCATCGACGCTATCATTAATATATATAAGCCCGCATCTA" ],
	  reads  => [ "CACGATCGACTTGC".     "TCATCGACGCTATCATTAATATATATAAGCCCGCATCTA" ],
	  #            0123456789012        012345678901234567890123456789012345678
	  hits   => [ { 0 => 1 } ],
	  samoptflags => [ {
		"AS:i:-12"       => 1, # alignment score
		"XN:i:3"         => 1, # num ambiguous ref bases
		"XM:i:1"         => 1, # num mismatches
		"XO:i:1"         => 1, # num gap opens
		"XG:i:2"         => 1, # num gap extensions
		"NM:i:3"         => 1, # num edits
		"MD:Z:13^NN0N39" => 1, # mismatching positions/bases
		"YT:Z:UU"        => 1, # type of alignment (concordant/discordant/etc)
	} ] },

	#
	# Local alignment
	#

	# Local alignment for a short perfect hit where hit spans the read
	{ name   => "Local alignment 1",
	  ref    => [ "TTGT" ],
	  reads  => [ "TTGT" ],
	  args   =>   "--local --policy \"MIN=L,1.0,0.75\"",
	  hits   => [ { 0 => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU,XC:4=" ],
	  cigar  => [ "4M" ],
	  samoptflags => [ {
		"AS:i:8"   => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:4"   => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YP:i:0"   => 1, # read aligned repetitively in paired fashion
		"YT:Z:UU"  => 1, # type of alignment (concordant/discordant/etc)
	} ] },

	#   T T G A     T T G A
	# T x         T   x
	# T   x       T
	# G     x     G
	# T           T

	# Local alignment for a short hit where hit is trimmed at one end
	{ name   => "Local alignment 2",
	  ref    => [ "TTGA" ],
	  reads  => [ "TTGT" ],
	  args   =>   "--local --policy \"MIN=L,1.0,0.75\\;SEED=0\\;SEEDLEN=3\\;IVAL=C,1,0\"",
	  report =>   "-a",
	  hits   => [ { 0 => 1 } ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:3=1S" ],
	  cigar  => [ "3M1S" ],
	  samoptflags => [ {
		"AS:i:6"   => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:3"   => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YP:i:0"   => 1, # read aligned repetitively in paired fashion
		"YT:Z:UU"  => 1, # type of alignment (concordant/discordant/etc)
	} ] },

	#     0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5
	#     T T G T T C G T T T G T T C G T
	# 0 T x
	# 1 T   x
	# 2 G     x
	# 3 T       x
	# 4 T         x
	# 5 C           x
	# 6 G             x
	# 7 T               x
	# 8 T                 x
	# 9 T                   x
	# 0 G                     x
	# 1 T                       x
	# 2 T                         x
	#
	# Score=130

	#     0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5
	#     T T G T T C G T T T G T T C G T
	# 0 T                 x
	# 1 T                   x
	# 2 G                     x
	# 3 T                       x
	# 4 T                         x
	# 5 C                           x
	# 6 G                             x
	# 7 T                               x
	# 8 T
	# 9 T
	# 0 G
	# 1 T
	# 2 T
	#
	# Score=80

	# Local alignment for a perfect hit
	{ name   => "Local alignment 3",
	  #            TTGTTCGT
	  #                    TTGTTCGT
	  ref    => [ "TTGTTCGTTTGTTCGT" ],
	  #            0123456789012345
	  #            TTGTTCGTTTGTT
	  #                    TTGTTCGT-----
	  reads  => [ "TTGTTCGTTTGTT" ],
	  args   =>   "--local -L 8 -i C,1,0 --score-min=C,12",
	  report =>   "-a",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  flags_map => [{
		0 => "XM:0,XP:0,XT:UU,XC:13=",
		8 => "XM:0,XP:0,XT:UU,XC:8="
	  }],
	  cigar_map => [{
		0 => "13M",
		8 => "8M5S"
	  }],
	  samoptflags_map => [{
		0 => { "AS:i:26" => 1, "XS:i:16" => 1, "YT:Z:UU" => 1, "MD:Z:13" => 1 },
		8 => { "AS:i:16" => 1, "XS:i:16" => 1, "YT:Z:UU" => 1, "MD:Z:8"  => 1 }
	  }]
	},

	#                          1
	#      0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5
	#      T T G T T C G T T T G T T C G T
	#  0 T                 x
	#  1 T                   x
	#  2 G                     x
	#  3 T                       x
	#  4 T                         x
	#  5 C                           x
	#  6 G                             x
	#  7 T                               x
	#  8 T
	#  9 T
	# 10 G
	#  1 T

	# Local alignment for a hit that should be trimmed from the right end
	{ name   => "Local alignment 4",
	  ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCGTTTGT" ],
	  args   =>   "--local --policy \"SEED=0\\;SEEDLEN=3\\;IVAL=C,1,0\" --score-min=C,12",
	  report =>   "-a",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  flags_map => [{
		0 => "XM:0,XP:0,XT:UU,XC:12=",
		8 => "XM:0,XP:0,XT:UU,XC:8=4S"
	  }],
	  cigar_map => [{
		0 => "12M",
		8 => "8M4S"
	  }],
	  samoptflags_map => [{
		0 => { "AS:i:24" => 1, "XS:i:16" => 1, "YT:Z:UU" => 1, "MD:Z:12" => 1 },
		8 => { "AS:i:16" => 1, "XS:i:16" => 1, "YT:Z:UU" => 1, "MD:Z:8"  => 1 }
	  }]
	},

	#
	# Test some common featuers for the manual.  E.g. when more than one
	# alignment is reported in -k mode, what order are they reported in?  They
	# should be in order by alignment score.
	#

	{ name   => "Alignment order -k",
	#              012345678
	  ref    => [ "GCGCATGCACATATCANNNNNGCGCATGCACATATCTNNNNNNNNGCGCATGCACATATTTNNNNNNNNNGCGCATGGTGTTATCA" ],
	  reads  => [ "GCGCATGCACATATCA" ],
	  quals  => [ "GOAIYEFGFIWDSFIU" ],
	  args   => "--min-score C,-24,0 -L 4",
	  report => "-k 4"
	},

	{ name   => "Alignment order -a",
	#              012345678
	  ref    => [ "GCGCATGCACATATCANNNNNGCGCATGCACATATCTNNNNNNNNGCGCATGCACATATTTNNNNNNNNNGCGCATGGTGTTATCA" ],
	  reads  => [ "GCGCATGCACATATCA" ],
	  quals  => [ "GOAIYEFGFIWDSFIU" ],
	  args   => "--min-score C,-24,0 -L 4",
	  report => "-a"
	},

	#
	# What order are mates reported in?  Should be reporting in mate1/mate2
	# order.
	#

	{ name   => "Mate reporting order, -a",
	#              012345678
	  ref    => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAAATAGACGACTCGATCGCGGATTAGGGGTAGACCCCCCCCCGACTNNNNNNNNNNAGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAAATAGACGACTCGATCGCGGATTAGGGGTAGACCCCCCCCCGACTNNNNNNNNNNAGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAAATAGACGACTCGATCGCGGATTAGGGGTAGACCCCCCCCCGACTNNNNNNNNCGGTAATACGGCCATCGCGGCGGCATTACTCGGCGACTGCACGAGCAGATATTGGGGGTCTAATATAACGTCTCATTAAAACGCTCTAGTCAGCTCATTGGCTCTA" ],
	  mate1s => [ "CTATCATCACGCGGATATT", "GGGGGGGGTCTACCCCTAA", "ATACGGCCATCGCGGCGGCATTACTCGGCG" ],
	  mate2s => [ "GGGGGGGGTCTACCCCTAA", "CTATCATCACGCGGATATT", "AGCCAATGAGCTGACTAGAGCGTTTT" ],
	  quals  => [ "GOAIYEFGFIWDSFIU" ],
	  args   => "",
	  report => "-a"
	},

	{ name   => "Mate reporting order, -M 1",
	#              012345678
	  ref    => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAAATAGACGACTCGATCGCGGATTAGGGGTAGACCCCCCCCCGACTNNNNNNNNNNAGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAAATAGACGACTCGATCGCGGATTAGGGGTAGACCCCCCCCCGACTNNNNNNNNNNAGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAAATAGACGACTCGATCGCGGATTAGGGGTAGACCCCCCCCCGACTNNNNNNNNCGGTAATACGGCCATCGCGGCGGCATTACTCGGCGACTGCACGAGCAGATATTGGGGGTCTAATATAACGTCTCATTAAAACGCTCTAGTCAGCTCATTGGCTCTA" ],
	  mate1s => [ "CTATCATCACGCGGATATT", "GGGGGGGGTCTACCCCTAA", "ATACGGCCATCGCGGCGGCATTACTCGGCG" ],
	  mate2s => [ "GGGGGGGGTCTACCCCTAA", "CTATCATCACGCGGATATT", "AGCCAATGAGCTGACTAGAGCGTTTT" ],
	  quals  => [ "GOAIYEFGFIWDSFIU" ],
	  args   => "",
	  report => "-M 1"
	},

	#
	# Test dovetailing, containment, and overlapping
	#
	{ name     => "Non-overlapping; no args",
	  ref      => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAA" ],
	  #              01234567890123456789012345678901234567890123456
	  mate1s   => [  "GCTATCATCACGCGGATA"                        ],
	  mate2s   => [                        "CGCATCGACATTAATATCC" ],
	  pairhits => [{ "1,23" => 1 }],
	  mate1fw  => 1, mate2fw => 1,
	  report   => "-M 1"
	},
	{ name     => "Non-overlapping; --no-discordant",
	  ref      => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAA" ],
	  #              01234567890123456789012345678901234567890123456
	  mate1s   => [  "GCTATCATCACGCGGATA"                        ],
	  mate2s   => [                        "CGCATCGACATTAATATCC" ],
	  pairhits => [{ "1,23" => 1 }],
	  mate1fw  => 1, mate2fw => 1,
	  report   => "-M 1 --no-discordant"
	},
	{ name     => "Non-overlapping; --no-discordant --no-mixed",
	  ref      => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAA" ],
	  #              01234567890123456789012345678901234567890123456
	  mate1s   => [  "GCTATCATCACGCGGATA"                        ],
	  mate2s   => [                        "CGCATCGACATTAATATCC" ],
	  pairhits => [{ "1,23" => 1 }],
	  mate1fw  => 1, mate2fw => 1,
	  report   => "-M 1 --no-discordant --no-mixed"
	},
	{ name     => "Non-overlapping; --no-discordant --no-mixed",
	  ref      => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAA" ],
	  #              01234567890123456789012345678901234567890123456
	  mate1s   => [  "GCTATCATCACGCGGATA"                        ],
	  mate2s   => [                        "CGCATCGACATTAATATCC" ],
	  pairhits => [{ "1,23" => 1 }],
	  mate1fw  => 1, mate2fw => 1,
	  report   => "-M 1 --no-discordant --no-mixed"
	},
	{ name     => "Non-overlapping; --no-dovetail",
	  ref      => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAA" ],
	  #              01234567890123456789012345678901234567890123456
	  mate1s   => [  "GCTATCATCACGCGGATA"                        ],
	  mate2s   => [                        "CGCATCGACATTAATATCC" ],
	  pairhits => [{ "1,23" => 1 }],
	  mate1fw  => 1, mate2fw => 1,
	  args     => "--no-dovetail",
	  report   => "-M 1"
	},
	{ name     => "Non-overlapping; --un-conc=.tmp.simple_tests.pl",
	  ref      => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAA" ],
	  #              01234567890123456789012345678901234567890123456
	  mate1s   => [  "GCTATCATCACGCGGATA"                        ],
	  mate2s   => [                        "CGCATCGACATTAATATCC" ],
	  pairhits => [{ "1,23" => 1 }],
	  mate1fw  => 1, mate2fw => 1,
	  args     => "--un-conc=.tmp.simple_tests.pl",
	  report   => "-M 1"
	},
	{ name     => "Non-overlapping; --no-overlap",
	  ref      => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAA" ],
	  #              01234567890123456789012345678901234567890123456
	  mate1s   => [  "GCTATCATCACGCGGATA"                        ],
	  mate2s   => [                        "CGCATCGACATTAATATCC" ],
	  pairhits => [{ "1,23" => 1 }],
	  mate1fw  => 1, mate2fw => 1,
	  args     => "--no-overlap",
	  report   => "-M 1"
	},

	{ name     => "Overlapping; no args",
	  ref      => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAA" ],
	  #              01234567890123456789012345678901234567890123456
	  mate1s   => [  "GCTATCATCACGCGGATATTA"                        ],
	  mate2s   => [                    "TTAGCGCATCGACATTAATATCC" ],
	  pairhits => [{ "1,19" => 1 }],
	  mate1fw  => 1, mate2fw => 1,
	  args     => "",
	  report   => "-M 1"
	},
	{ name     => "Overlapping; --no-dovetail",
	  ref      => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAA" ],
	  #              01234567890123456789012345678901234567890123456
	  mate1s   => [  "GCTATCATCACGCGGATATTA"                        ],
	  mate2s   => [                    "TTAGCGCATCGACATTAATATCC" ],
	  pairhits => [{ "1,19" => 1 }],
	  mate1fw  => 1, mate2fw => 1,
	  args     => "--no-dovetail",
	  report   => "-M 1"
	},
	{ name     => "Overlapping; --no-contain",
	  ref      => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAA" ],
	  #              01234567890123456789012345678901234567890123456
	  mate1s   => [  "GCTATCATCACGCGGATATTA"                        ],
	  mate2s   => [                    "TTAGCGCATCGACATTAATATCC" ],
	  pairhits => [{ "1,19" => 1 }],
	  mate1fw  => 1, mate2fw => 1,
	  args     => "--no-contain",
	  report   => "-M 1"
	},
	{ name     => "Overlapping; --no-overlap",
	  ref      => [ "AGCTATCATCACGCGGATATTAGCGCATCGACATTAATATCCCCAAA" ],
	  #              01234567890123456789012345678901234567890123456
	  mate1s   => [  "GCTATCATCACGCGGATATTA"                        ],
	  mate2s   => [                    "TTAGCGCATCGACATTAATATCC" ],
	  pairhits => [],
	  mate1fw  => 1, mate2fw => 1,
	  args     => "--no-overlap",
	  report   => "-M 1"
	},

	#
	# Test XS:i with quality scaling
	#

	{ name   => "Scoring params 1",
	#              012345678
	  ref    => [ "ACTATTGCGCGCATGCACATATCAATTAAGCCGTCTCTCTAAAGAGACCCCAATCTCGCGCGCTAGACGTCAGTAGTTTAATTTTATAAACACCTCGCTGCGGGG" ],
	  reads  => [         "GCGCATGCACATATCAATTAAGCCGTCTCTCTAAAGAGACCCCAATCTCGCGCGCTAGACGTCAGTAGTTTAATTTTATAAACACCTC" ],
	  quals  => [         "GOAIYEFGFIWDSFIUYWEHRIWQWLFNSLDKkjdfglduhiuevhsiuqkAUHFIUEHGIUDJFHSKseuweyriwfskdgbiuuhh" ],
	  #                    0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567
	  #                    0         1         2         3         4         5         6         7         8
	  args   => "",
	  report => "-M 1",
	  hits   => [ { 8 => 1 } ],
	  cigar  => [ "88M" ],
	  samoptflags => [ {
		  "AS:i:0"   => 1,
		  "YT:Z:UU"  => 1,
		  "MD:Z:88" => 1 } ],
	},

	{ name   => "Scoring params 2",
	#              012345678
	  ref    => [ "ACTATTGCGCGCATGCACATATCAATTAAGCCGTCTCTCTAAAGAGACCCCAATCTCGCGCGCTAGACGTCAGTAGTTT"."TTTATAAACACCTCGCTGCGGGG" ],
	  reads  => [         "NCGCATGCACATtTCAATTAAGCCGTCTCTCTAAAGA". "CCAATCTCGCGCGCTAGACGTCAGTAGTTTAAATTTATAAACACCTC" ],
	  #                    * -1        * -6                     **** -5 -3 -3 -3 -3               *** -5 -3 -3 -3
	  quals  => [         "GOAIYEFGFIWDSFIUYWEHRIWQWLFNSLDKkjdfg". "iuevhsiuqkAUHFIUEHGIUDJFHSKseuweyriwfskdgbiuuhh" ],
	  #                    0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567
	  #                    0         1         2         3         4         5         6         7         8
	  args   => "--ignore-quals --score-min C,-40,0 -N 1 -L 20",
	  report => "-M 1",
	  hits   => [ { 8 => 1 } ],
	  cigar  => [ "37M4D30M3I14M" ],
	  #            37M4D30M13I4M
	  samoptflags => [ {
		  "AS:i:-38" => 1,
		  "YT:Z:UU"  => 1,
		  "MD:Z:0G11A24^GACC44" => 1,
		  "NM:i:9"   => 1,
		  "XM:i:2"   => 1,
		  "XG:i:7"   => 1,
		  "XO:i:2"   => 1 } ],
	},

	{ name   => "Scoring params 3",
	#              012345678
	  ref    => [ "ACTATTGCGCGCATGCACATATCAATTAAGCCGTCTCTCTAAAGAGACCCCAATCTCGCGCGCTAGACGTCAGTAGTTT"."TTTATAAACACCTCGCTGCGGGG" ],
	  reads  => [         "NCGCATGCACATtTCAATTAAGCCGTCTCTCTAAAGA". "CCAATCTCGCGCGCTAGACGTCAGTAGTTTAAATTTATAAACACCTC" ],
	  #                    * -1        * -6                     **** -5 -3 -3 -3 -3               *** -1 -2 -2 -2
	  quals  => [         "GOAIYEFGFIWDSFIUYWEHRIWQWLFNSLDKkjdfg". "iuevhsiuqkAUHFIUEHGIUDJFHSKseuweyriwfskdgbiuuhh" ],
	  #                    0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567
	  #                    0         1         2         3         4         5         6         7         8
	  args   => "--ignore-quals --rfg 1,2 --score-min C,-40,0 -N 1 -L 20",
	  report => "-M 1",
	  hits   => [ { 8 => 1 } ],
	  cigar  => [ "37M4D30M3I14M" ],
	  samoptflags => [ {
		  "AS:i:-31" => 1,
		  "YT:Z:UU"  => 1,
		  "MD:Z:0G11A24^GACC44" => 1,
		  "NM:i:9"   => 1,
		  "XM:i:2"   => 1,
		  "XG:i:7"   => 1,
		  "XO:i:2"   => 1 } ],
	},

	{ name   => "Scoring params 4",
	#              012345678
	  ref    => [ "ACTATTGCGCGCATGCACATATCAATTAAGCCGTCTCTCTAAAGAGACCCCAATCTCGCGCGCTAGACGTCAGTAGTTT"."TTTATAAACACCTCGCTGCGGGG" ],
	  reads  => [         "NCGCATGCACATtTCAATTAAGCCGTCTCTCTAAAGA". "CCAATCTCGCGCGCTAGACGTCAGTAGTTTAAATTTATAAACACCTC" ],
	  #                    * -1        * -6                     **** -1 -2 -2 -2 -2               *** -5 -3 -3 -3
	  quals  => [         "GOAIYEFGFIWDSFIUYWEHRIWQWLFNSLDKkjdfg". "iuevhsiuqkAUHFIUEHGIUDJFHSKseuweyriwfskdgbiuuhh" ],
	  #                    0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567
	  #                    0         1         2         3         4         5         6         7         8
	  args   => "--ignore-quals --rdg 1,2 --score-min C,-40,0 -N 1 -L 20",
	  report => "-M 1",
	  hits   => [ { 8 => 1 } ],
	  cigar  => [ "37M4D30M3I14M" ],
	  samoptflags => [ {
		  "AS:i:-30" => 1,
		  "YT:Z:UU"  => 1,
		  "MD:Z:0G11A24^GACC44" => 1,
		  "NM:i:9"   => 1,
		  "XM:i:2"   => 1,
		  "XG:i:7"   => 1,
		  "XO:i:2"   => 1 } ],
	},

	{ name   => "Scoring params 5",
	#              012345678
	  ref    => [ "ACTATTGCGCGCATGCACATATCAATTAAGCCGTCTCTCTAAAGAGACCCCAATCTCGCGCGCTAGACGTCAGTAGTTT"."TTTATAAACACCTCGCTGCGGGG" ],
	  reads  => [         "NCGCATGCACATtTCAATTAAGCCGTCTCTCTAAAGA". "CCAATCTCGCGCGCTAGACGTCAGTAGTTTAAATTTATAAACACCTC" ],
	  #                    * -1        * -8                     **** -5 -3 -3 -3 -3               *** -5 -3 -3 -3
	  quals  => [         "GOAIYEFGFIWDSFIUYWEHRIWQWLFNSLDKkjdfg". "iuevhsiuqkAUHFIUEHGIUDJFHSKseuweyriwfskdgbiuuhh" ],
	  #                    0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567
	  #                    0         1         2         3         4         5         6         7         8
	  args   => "--ignore-quals --mp 8 --score-min C,-40,0 -N 1 -L 20",
	  report => "-M 1",
	  hits   => [ { 8 => 1 } ],
	  cigar  => [ "37M4D30M3I14M" ],
	  samoptflags => [ {
		  "AS:i:-40" => 1,
		  "YT:Z:UU"  => 1,
		  "MD:Z:0G11A24^GACC44" => 1,
		  "NM:i:9"   => 1,
		  "XM:i:2"   => 1,
		  "XG:i:7"   => 1,
		  "XO:i:2"   => 1 } ],
	},

	{ name   => "Scoring params 6",
	#              012345678
	  ref    => [ "ACTATTGCGCGCATGCACATATCAATTAAGCCGTCTCTCTAAAGAGACCCCAATCTCGCGCGCTAGACGTCAGTAGTTT"."TTTATAAACACCTCGCTGCGGGG" ],
	  reads  => [         "NCGCATGCACATtTCAATTAAGCCGTCTCTCTAAAGA". "CCAATCTCGCGCGCTAGACGTCAGTAGTTTAAATTTATAAACACCTC" ],
	  #                    * -4        * -6                     **** -5 -3 -3 -3 -3               *** -5 -3 -3 -3
	  quals  => [         "GOAIYEFGFIWDSFIUYWEHRIWQWLFNSLDKkjdfg". "iuevhsiuqkAUHFIUEHGIUDJFHSKseuweyriwfskdgbiuuhh" ],
	  #                    0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567
	  #                    0         1         2         3         4         5         6         7         8
	  args   => "--ignore-quals --np 4 --score-min C,-41,0 -N 1 -L 20",
	  report => "-M 1",
	  hits   => [ { 8 => 1 } ],
	  cigar  => [ "37M4D30M3I14M" ],
	  samoptflags => [ {
		  "AS:i:-41" => 1,
		  "YT:Z:UU"  => 1,
		  "MD:Z:0G11A24^GACC44" => 1,
		  "NM:i:9"   => 1,
		  "XM:i:2"   => 1,
		  "XG:i:7"   => 1,
		  "XO:i:2"   => 1 } ],
	},

	#
	# Test XS:i with quality scaling
	#

	{ name   => "Q XS:i 1a",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  quals  => [ "IIIIIIIA" ],
	  args   => "--multiseed=0,7,C,1 --score-min=C,-6",
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:-5"  => 1, "XS:i:-5" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:7A0" => 1,
		  "NM:i:1"   => 1, "XM:i:1"  => 1 } ],
	},

	{ name   => "Q XS:i 1a ! --mp 3,3",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  quals  => [ "IIIIIII!" ],
	  args   => "-L 6 --mp 3,3 --score-min=C,-6",
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:-3"  => 1, "XS:i:-3" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:7A0" => 1,
		  "NM:i:1"   => 1, "XM:i:1"  => 1 } ],
	},

	{ name   => "Q XS:i 1a ! --mp 3,6",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  quals  => [ "IIIIIII!" ],
	  args   => "-L 6 --mp 6,3 --score-min=C,-6",
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:-3"  => 1, "XS:i:-3" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:7A0" => 1,
		  "NM:i:1"   => 1, "XM:i:1"  => 1 } ],
	},

	{ name   => "Q XS:i 1a I --mp 3,3",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  quals  => [ "IIIIIIII" ],
	  args   => "-L 6 --mp 3,3 --score-min=C,-6",
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:-3"  => 1, "XS:i:-3" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:7A0" => 1,
		  "NM:i:1"   => 1, "XM:i:1"  => 1 } ],
	},

	{ name   => "Q XS:i 1a I --mp 3,6",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  quals  => [ "IIIIIIII" ],
	  args   => "-L 6 --mp 6,3 --score-min=C,-6",
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:-6"  => 1, "XS:i:-6" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:7A0" => 1,
		  "NM:i:1"   => 1, "XM:i:1"  => 1 } ],
	},

	{ name   => "Q XS:i 1a --ignore-quals",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  quals  => [ "IIIIIIIA" ],
	  args   => "--multiseed=0,7,C,1 --score-min=C,-6 --ignore-quals",
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:-6"  => 1, "XS:i:-6" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:7A0" => 1,
		  "NM:i:1"   => 1, "XM:i:1"  => 1 } ],
	},

	{ name   => "Q XS:i 1b",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  quals  => [ "IIIIIII5" ],
	  args   => "--multiseed=0,7,C,1 --score-min=C,-6",
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:-4"  => 1, "XS:i:-4" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:7A0" => 1,
		  "NM:i:1"   => 1, "XM:i:1"  => 1 } ],
	},

	{ name   => "Q XS:i 1b --ignore-quals",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  quals  => [ "IIIIIII5" ],
	  args   => "--multiseed=0,7,C,1 --score-min=C,-6 --ignore-quals",
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:-6"  => 1, "XS:i:-6" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:7A0" => 1,
		  "NM:i:1"   => 1, "XM:i:1"  => 1 } ],
	},

	{ name   => "Q XS:i 1c",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  quals  => [ "IIIIIII4" ],
	  args   => "--multiseed=0,7,C,1 --score-min=C,-6",
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:-3"  => 1, "XS:i:-3" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:7A0" => 1,
		  "NM:i:1"   => 1, "XM:i:1"  => 1 } ],
	},

	{ name   => "Q XS:i 1c --ignore-quals",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  quals  => [ "IIIIIII4" ],
	  args   => "--multiseed=0,7,C,1 --score-min=C,-6 --ignore-quals",
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:-6"  => 1, "XS:i:-6" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:7A0" => 1,
		  "NM:i:1"   => 1, "XM:i:1"  => 1 } ],
	},

	# One mate aligns.  Ensuring that the unmapped mate gets reference
	# information filled in from the other mate.
	{ ref    => [ "CATCGACTGAGACTCGTACGACAATTACGCGCATTATTCGCATCACCAGCGCGGCGCGCGCCCCCTAT" ],
	#              01234567890123456789012345678901234567890123456789012345678901234567
	#              0         1         2         3         4         5         6
	#                                                       ATCACCAGCGTTTCGCGCGAAACCTA
	  mate1s => [ "ATCGACTGAGACTCGTACGACAATTAC" ],
	  mate2s => [ "TAGGTTTCGCGCGAAACGCTGGTGAT" ],
	  pairhits_orig => [{ "1,1" => 1}]
	},

	{ ref    => [ "TTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCGT" ],
	  args   => "--multiseed=0,4,C,1,0",
	  report => "-M 1"
	},

	# Testing that DEFAULT is -M 1
	{ ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCGT" ],
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [
		{ "YM:i:1" => 1, "YT:Z:UU" => 1, "MD:Z:8" => 1, "YM:i:1" => 1 }
	  ],
	},
	{ ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCGT" ],
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [
		{ "YM:i:1" => 1, "YT:Z:UU" => 1, "MD:Z:8" => 1, "YM:i:1" => 1 }
	  ],
	},

	#
	# Test XS:i
	#

	{ name   => "XS:i 1",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  args   => "--multiseed=0,7,C,1 --score-min=C,-6",
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:-6"  => 1, "XS:i:-6" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:7A0" => 1,
		  "NM:i:1"   => 1, "XM:i:1"  => 1 } ],
	},

	{ name   => "XS:i 2",
	  ref    => [ "TTGTTCGATTGTTCGA" ],
	  reads  => [ "TTGTTCGT" ],
	  args   => "--multiseed=0,7,C,1 --score-min=C,-5",
	  report => "",
	  cigar  => [ "*" ],
	  samoptflags => [{ "YT:Z:UU" => 1, "YM:i:0" => 1 }],
	},

	{ name   => "XS:i 3a",
	  ref    => [ "TTGTTCGATTGTTCGT" ],
	  #                    TTGTTCGT
	  reads  => [ "TTGTTCGT" ],
	  args   => "--multiseed=0,7,C,1 --score-min=C,-6",
	  report => "-M 1",
	  hits   => [ { 8 => 1 } ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:0"   => 1, "XS:i:-6" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:8"   => 1,
		  "NM:i:0"   => 1, "XM:i:0"  => 1 } ],
	},

	{ name   => "XS:i 3b",
	  ref    => [ "TTGTTCGATTGTTCGT" ],
	  #                    TTGTTCGT
	  reads  => [ "TTGTTCGT" ],
	  args   => "--multiseed=0,7,C,1 --score-min=C,-6 --seed=52",
	  report => "-M 1",
	  hits   => [ { 8 => 1 } ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:0"   => 1, "XS:i:-6" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:8"   => 1,
		  "NM:i:0"   => 1, "XM:i:0"  => 1 } ],
	},

	{ name   => "XS:i 3c",
	  ref    => [ "TTGTTCGATTGTTCGT" ],
	  #                    TTGTTCGT
	  reads  => [ "TTGTTCGT" ],
	  args   => "--multiseed=0,7,C,1 --score-min=C,-6 --seed=53",
	  report => "-M 2",
	  hits   => [ { 8 => 1 } ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:0"   => 1, "XS:i:-6" => 1,
		  "YM:i:0"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:8"   => 1,
		  "NM:i:0"   => 1, "XM:i:0"  => 1 } ],
	},

	{ name   => "XS:i 4a",
	  ref    => [ "TTGTTCAATTGTTCGATTGTTCGT" ],
	  #            ||||||  ||||||| ||||||||
	  #            TTGTTCGT||||||| ||||||||
	  #                    TTGTTCGT||||||||
	  #                            TTGTTCGT
	  reads  => [ "TTGTTCGT" ],
	  args   => "--multiseed=0,6,C,1 --score-min=C,-12 --seed=53",
	  report => "-M 2",
	  hits   => [ { 16 => 1 } ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:0"   => 1, "XS:i:-6" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:8"   => 1,
		  "NM:i:0"   => 1, "XM:i:0"  => 1 } ],
	},

	{ name   => "XS:i 4b",
	  ref    => [ "TTGTTCAATTGTTCGATTGTTCGT" ],
	  #            ||||||  ||||||| ||||||||
	  #            TTGTTCGT||||||| ||||||||
	  #                    TTGTTCGT||||||||
	  #                            TTGTTCGT
	  reads  => [ "TTGTTCGT" ],
	  args   => "--multiseed=0,6,C,1 --score-min=C,-12 --seed=54",
	  report => "-M 3",
	  hits   => [ { 16 => 1 } ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:0"   => 1, "XS:i:-6" => 1,
		  "YM:i:0"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:8"   => 1,
		  "NM:i:0"   => 1, "XM:i:0"  => 1 } ],
	},

	{ name   => "XS:i 5a",
	  ref    => [ "TTGTTCAATTGTTCGATTGTTCGTTTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAA" ],
	  #            ||||||  ||||||| ||||||||||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||
	  #            TTGTTCGT||||||| ||||||||TTGTTCGT||||||  TTGTTCGT||||||  TTGTTCGT||||||  TTGTTCGT||||||  TTGTTCGT||||||  TTGTTCGT||||||
	  #                    TTGTTCGT||||||||        TTGTTCGT        TTGTTCGT        TTGTTCGT        TTGTTCGT        TTGTTCGT        TTGTTCGT
	  #                            TTGTTCGT
	  reads  => [ "TTGTTCGT" ],
	  args   => "--multiseed=0,6,C,1,1 --score-min=C,-12 --seed=54",
	  report => "-M 1",
	  hits   => [ { 16 => 1 } ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:0"   => 1, "XS:i:-6" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:8"   => 1,
		  "NM:i:0"   => 1, "XM:i:0"  => 1 } ],
	},

	{ name   => "XS:i 5b",
	  ref    => [ "TTGTTCAATTGTTCGATTGTTCGTTTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAATTGTTCAA" ],
	  #            ||||||  ||||||| ||||||||||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||  ||||||
	  #            TTGTTCGT||||||| ||||||||TTGTTCGT||||||  TTGTTCGT||||||  TTGTTCGT||||||  TTGTTCGT||||||  TTGTTCGT||||||  TTGTTCGT||||||
	  #                    TTGTTCGT||||||||        TTGTTCGT        TTGTTCGT        TTGTTCGT        TTGTTCGT        TTGTTCGT        TTGTTCGT
	  #                            TTGTTCGT
	  reads  => [ "TTGTTCGT" ],
	  args   => "--multiseed=0,5,C,1,1 --score-min=C,-12 --seed=55",
	  report => "-M 1",
	  hits   => [ { 16 => 1 } ],
	  cigar  => [ "8M" ],
	  samoptflags => [ {
		  "AS:i:0"   => 1, "XS:i:-6" => 1,
		  "YM:i:1"   => 1, "YT:Z:UU" => 1,
		  "MD:Z:8"   => 1,
		  "NM:i:0"   => 1, "XM:i:0"  => 1 } ],
	},

	# Testing BWA-SW-like scoring
	#
	# a*max{T,c*log(l)} = 1 * max(30, 5.5 * log(56)) = 1 * max(30, 22.139) = 30
	#
	{ name     => "BWA-SW-like 1",
	  ref      => [ "GTTTAGATTCCACTACGCTAACCATCGAGAACTCGTCTCAGAGTTTCGATAGGAAAATCTGCGA" ],
	  #                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
	  reads    => [    "TAGATTCCACTACGCTAACCATCGAGAACTCGTCTCAGAGTTTCGATAGGAAAATC" ],
	  #                 01234567890123456789012345678901234567890123456789012345
	  #                           1         2         3         4         5
	  args     => "--bwa-sw-like",
	  hits     => [{ 3 => 1 }],
	  samoptflags => [{ "AS:i:56" => 1, "NM:i:0" => 1,
	                    "MD:Z:56" => 1, "YT:Z:UU" => 1 }]
	},
	{ name     => "BWA-SW-like 2",
	  #              0123
	  ref      => [ "GTTTAGATTCCACTACGCTAACCATCGAGAACTCGTCTCAGAGTTTCGATAGGAAAATCTGCGA" ],
	  #                 ||||||||||||||||||||||||||  ||||||||||||||||||||||||||||
	  reads    => [    "TAGATTCCACTACGCTAACCATCGAGTTCTCGTCTCAGAGTTTCGATAGGAAAATC" ],
	  #                 01234567890123456789012345678901234567890123456789012345
	  #                           1         2         3         4         5
	  args     => "--bwa-sw-like -L 18",
	  hits     => [{ 3 => 1 }],
	  # Tot matches = 54
	  # Tot penalties = 6
	  samoptflags => [{ "AS:i:48" => 1, "NM:i:2" => 1, "XM:i:2" => 1,
	                    "MD:Z:26A0A28" => 1, "YT:Z:UU" => 1 }]
	},
	{ name     => "BWA-SW-like 3",
	  #              0123
	  ref      => [ "GTTTAGATTCCACTACGCTAACCATCGAGAACTCGTCTCAGAGTTTCGATAGGAAAATCTGCGA" ],
	  #                 ||||||||||||||||||||||||||   |||||||||||||||||||||||||||
	  reads    => [    "TAGATTCCACTACGCTAACCATCGAG"."TCGTCTCAGAGTTTCGATAGGAAAATC" ],
	  #                 01234567890123456789012345678901234567890123456789012345
	  #                           1         2         3         4         5
	  args     => "--bwa-sw-like -i C,1,0",
	  hits     => [{ 3 => 1 }],
	  # Tot matches = 53
	  # Tot penalties = 11
	  samoptflags => [{ "AS:i:42" => 1, "NM:i:3" => 1, "XM:i:0" => 1,
	                    "XO:i:1" => 1, "XG:i:3" => 1,
	                    "MD:Z:26^AAC27" => 1, "YT:Z:UU" => 1 }]
	},

	# Some tricky SAM FLAGS field tests

        { name     => "SAM paired-end where both alignments start at the same offset (fr case)",
	  ref      => [ "GCACTATCTACGCTTCGGCGTCGGCGAAAAAACGCACGACCGGGTGTGTGACAATCATATATAGCGCGC" ],
	  #              012345678901234567890123456789012345678901234567890123456789012345678
	  #              0         1         2         3         4         5         6
	  mate1s   => [    "CTATCTACGCTTCGGCGTCGGTGA" ],
          mate2s   => [    "TCACCGACGCCGAAGCGTAGATAG" ],
	  #                 -----------------------------------------------------
	  #                 01234567890123456789012345678901234567890123456789012
	  #                 0         1         2         3         4         5
	  #  0x1    template having multiple fragments in sequencing
	  #  0x2    each fragment properly aligned according to the aligner
	  #  0x4    fragment unmapped
	  #  0x8    next fragment in the template unmapped
	  # 0x10    SEQ being reverse complemented
	  # 0x20    SEQ of the next fragment in the template being reversed
	  # 0x40    the first fragment in the template
	  # 0x80    the last fragment in the template
	  pairhits     => [{ "3,3" => 1 }],
	  norc         => 1,
	  samflags_map => [{ 3 => [99, 147] }],
	  tlen_map     => [{ 3 => [24, -24] }] },

        { name     => "SAM paired-end where both alignments start at the same offset (ff case)",
	  ref      => [ "GCACTATCTACGCTTCGGCGTCGGCGAAAAAACGCACGACCGGGTGTGTGACAATCATATATAGCGCGC" ],
	  #              012345678901234567890123456789012345678901234567890123456789012345678
	  #              0         1         2         3         4         5         6
	  mate1s   => [    "CTATCTACGCTTCGGCGTCGGTGA" ],
          mate2s   => [    "CTATCTACGCTTCGGCGTCGGTGA" ],
	  #                 -----------------------------------------------------
	  #                 01234567890123456789012345678901234567890123456789012
	  #                 0         1         2         3         4         5
	  #  0x1    template having multiple fragments in sequencing
	  #  0x2    each fragment properly aligned according to the aligner
	  #  0x4    fragment unmapped
	  #  0x8    next fragment in the template unmapped
	  # 0x10    SEQ being reverse complemented
	  # 0x20    SEQ of the next fragment in the template being reversed
	  # 0x40    the first fragment in the template
	  # 0x80    the last fragment in the template
	  pairhits     => [{ "3,3" => 1 }],
          mate1fw      => 1,
          mate2fw      => 1,
          norc         => 1,
	  samflags_map => [{ 3 => [67, 131] }],
	  tlen_map     => [{ 3 => [24, -24] }] },

        { name     => "SAM paired-end where both alignments start at the same offset (rf case)",
	  ref      => [ "GCACTATCTACGCTTCGGCGTCGGCGAAAAAACGCACGACCGGGTGTGTGACAATCATATATAGCGCGC" ],
	  #              012345678901234567890123456789012345678901234567890123456789012345678
	  #              0         1         2         3         4         5         6
          mate1s   => [    "TCACCGACGCCGAAGCGTAGATAG" ],
          mate2s   => [    "CTATCTACGCTTCGGCGTCGGTGA" ],
	  #                 -----------------------------------------------------
	  #                 01234567890123456789012345678901234567890123456789012
	  #                 0         1         2         3         4         5
	  #  0x1    template having multiple fragments in sequencing
	  #  0x2    each fragment properly aligned according to the aligner
	  #  0x4    fragment unmapped
	  #  0x8    next fragment in the template unmapped
	  # 0x10    SEQ being reverse complemented
	  # 0x20    SEQ of the next fragment in the template being reversed
	  # 0x40    the first fragment in the template
	  # 0x80    the last fragment in the template
	  pairhits     => [{ "3,3" => 1 }],
          mate1fw      => 0,
          mate2fw      => 1,
          norc         => 1,
	  samflags_map => [{ 3 => [83, 163] }],
	  tlen_map     => [{ 3 => [-24, 24] }] },

        { name     => "SAM paired-end where both mates align 1",
	  ref      => [ "GCACTATCTACGCTTCGGCGTCGGCGAAAAAACGCACGACCGGGTGTGTGACAATCATATATAGCGCGC" ],
	  #              012345678901234567890123456789012345678901234567890123456789012345678
	  #              0         1         2         3         4         5         6
	  mate1s   => [    "CTATCTACGCTTCGGCGTCGGTGA" ],
	  mate2s   =>                                    [ "GATTGTCACACACCCGGTCGT" ],
	  #                 -----------------------------------------------------
	  #                 01234567890123456789012345678901234567890123456789012
	  #                 0         1         2         3         4         5
	  #  0x1    template having multiple fragments in sequencing
	  #  0x2    each fragment properly aligned according to the aligner
	  #  0x4    fragment unmapped
	  #  0x8    next fragment in the template unmapped
	  # 0x10    SEQ being reverse complemented
	  # 0x20    SEQ of the next fragment in the template being reversed
	  # 0x40    the first fragment in the template
	  # 0x80    the last fragment in the template
	  pairhits     => [{ "3,35" => 1 }],
	  norc         => 1,
	  samflags_map => [{ 3 => (1 | 2 | 32 | 64), 35 => (1 | 2 | 16 | 128) }],
	  tlen_map     => [{ 3 => 53, 35 => -53 }] },

	{ name     => "SAM paired-end where both mates align 2",
	  ref      => [ "GCACTATCTACGCTTCGGCGTCGGCGAAAAAACGCACGACCGGGTGTGTGACAATCATATATAGCGCGC" ],
	  #              012345678901234567890123456789012345678901234567890123456789012345678
	  #              0         1         2         3         4         5         6
	  mate1s   => [    "TCACCGACGCCGAAGCGTAGATAG" ],
	  mate2s   =>                                    [ "ACGACCGGGTGTGTGACAATC" ],
	  #                 -----------------------------------------------------
	  #                 01234567890123456789012345678901234567890123456789012
	  #                 0         1         2         3         4         5
	  #  0x1    template having multiple fragments in sequencing
	  #  0x2    each fragment properly aligned according to the aligner
	  #  0x4    fragment unmapped
	  #  0x8    next fragment in the template unmapped
	  # 0x10    SEQ being reverse complemented
	  # 0x20    SEQ of the next fragment in the template being reversed
	  # 0x40    the first fragment in the template
	  # 0x80    the last fragment in the template
	  mate1fw      => 0,
	  mate2fw      => 1,
	  pairhits     => [{ "3,35" => 1 }],
	  norc         => 1,
	  samflags_map => [{ 3 => (1 | 2 | 16 | 64), 35 => (1 | 2 | 32 | 128) }],
	  tlen_map     => [{ 3 => 53, 35 => -53 }] },

	{ name     => "SAM paired-end where both mates align 3",
	  ref      => [ "GCACTATCTACGCTTCGGCGTCGGCGAAAAAACGCACGACCGGGTGTGTGACAATCATATATAGCGCGC" ],
	  #              012345678901234567890123456789012345678901234567890123456789012345678
	  #              0         1         2         3         4         5         6
	  mate1s   => [    "CTATCTACGCTTCGGCGTCGGTGA" ],
	  mate2s   =>                                    [ "ACGACCGGGTGTGTGACAATC" ],
	  #                 -----------------------------------------------------
	  #                 01234567890123456789012345678901234567890123456789012
	  #                 0         1         2         3         4         5
	  #  0x1    template having multiple fragments in sequencing
	  #  0x2    each fragment properly aligned according to the aligner
	  #  0x4    fragment unmapped
	  #  0x8    next fragment in the template unmapped
	  # 0x10    SEQ being reverse complemented
	  # 0x20    SEQ of the next fragment in the template being reversed
	  # 0x40    the first fragment in the template
	  # 0x80    the last fragment in the template
	  mate1fw      => 1,
	  mate2fw      => 1,
	  pairhits     => [{ "3,35" => 1 }],
	  norc         => 1,
	  samflags_map => [{ 3 => (1 | 2 | 64), 35 => (1 | 2 | 128) }],
	  tlen_map     => [{ 3 => 53, 35 => -53 }] },

	{ name     => "SAM paired-end where mate #1 aligns but mate #2 doesn't",
	  ref      => [ "GCACTATCTACGCTTCGGCGTCGGCGAAAAAACGCACGACCGGGTGTGTGACAATCATATATAGCGCGC" ],
	  #              012345678901234567890123456789012345678901234567890123456789012345678
	  #              0         1         2         3         4         5         6
	  mate1s   => [    "CTATCTACGCTTCGGCGTCGGCGA" ],
	  mate2s   =>                                    [ "GATTGTCTTTTCCCGGAAAAATCGT" ],
	  #  0x1    template having multiple fragments in sequencing
	  #  0x2    each fragment properly aligned according to the aligner
	  #  0x4    fragment unmapped
	  #  0x8    next fragment in the template unmapped
	  # 0x10    SEQ being reverse complemented
	  # 0x20    SEQ of the next fragment in the template being reversed
	  # 0x40    the first fragment in the template
	  # 0x80    the last fragment in the template
	  pairhits     => [{ "*,3" => 1 }],
	  norc         => 1,
	  samflags_map => [{ 3 => (1 | 8 | 64), "*" => (1 | 4 | 128) }] },

	{ name     => "SAM paired-end where neither mate aligns",
	  ref      => [ "GCACTATCTACGCTTCGGCGTCGGCGAAAAAACGCACGACCGGGTGTGTGACAATCATATATAGCGCGC" ],
	  #              012345678901234567890123456789012345678901234567890123456789012345678
	  #              0         1         2         3         4         5         6
	  mate1s   => [    "CTATATACGAAAAAGCGTCGGCGA" ],
	  mate2s   =>                                    [ "GATTGTCTTTTCCCGGAAAAATCGT" ],
	  #  0x1    template having multiple fragments in sequencing
	  #  0x2    each fragment properly aligned according to the aligner
	  #  0x4    fragment unmapped
	  #  0x8    next fragment in the template unmapped
	  # 0x10    SEQ being reverse complemented
	  # 0x20    SEQ of the next fragment in the template being reversed
	  # 0x40    the first fragment in the template
	  # 0x80    the last fragment in the template
	  pairhits     => [{ "*,*" => 1 }],
	  norc         => 1,
	  samoptflags_flagmap => [{
		(1 | 4 | 8 |  64) => { "YT:Z:UP" => 1 },
		(1 | 4 | 8 | 128) => { "YT:Z:UP" => 1 }
	}] },

	{ name     => "SAM paired-end where both mates align, but discordantly",
	  ref      => [ "GCACTATCTACGCTTCGGCGTCGGCGAAAAAACGCACGACCGGGTGTGTGACAATCATATATAGCGCGC" ],
	  #              012345678901234567890123456789012345678901234567890123456789012345678
	  #              0         1         2         3         4         5         6
	  mate1s   => [    "CTATCTACGCTTCGGCGTCGGCGA" ],
	  mate2s   =>                                    [ "ACGACCGGGTGTGTGACAATC" ],
	  #                 -----------------------------------------------------
	  #                 01234567890123456789012345678901234567890123456789012
	  #                 0         1         2         3         4         5
	  #  0x1    template having multiple fragments in sequencing
	  #  0x2    each fragment properly aligned according to the aligner
	  #  0x4    fragment unmapped
	  #  0x8    next fragment in the template unmapped
	  # 0x10    SEQ being reverse complemented
	  # 0x20    SEQ of the next fragment in the template being reversed
	  # 0x40    the first fragment in the template
	  # 0x80    the last fragment in the template
	  pairhits     => [{ "3,35" => 1 }],
	  norc         => 1,
	  samflags_map => [{ 3 => (1 | 64), 35 => (1 | 128) }],
	  # Which TLEN is right?  Depends on criteria for when to infer TLEN.  If
	  # criterion is mates are concordant, then it should be 0 here.  If the
	  # criterion is that both mates align to the same chromosome, should be
	  # +-53
	  #tlen_map     => [{ 3 => 0, 35 => 0 }] },
	  tlen_map     => [{ 3 => 53, 35 => -53 }] },

	{ name   => "matchesRef regression 4",
	  ref    => [ "CCGGGTCGTCACGCCCCGCTTGCGTCANGCCCCTCACCCTCCCTTTGTCGGCTCCCACCCCTCCCCATCCGTTGTCCCCGCCCCCGCCCGCCGGGTCGTCACGCCCCGCTTGCGTCANGC",
	              "GCTCGGAATTCGTGCTCCGNCCCGTACGGTT" ],
	  #
	  #       NNNNNGA------A-------------------G-NTTT
	  #            ||||||||||||||||||||||||||||||||||
	  #       CCAAT-ATTTTTAATTTCCTCTATTTTTCTCTCGTCTTG
	  args   => "--policy \"NP=Q\\;RDG=46.3220993654702\\;RFG=41.3796024365659\\;MIN=L,5.57015383125426,-3.28597145122829\\;NCEIL=L,0.263054599454459,0.130843661549367\\;SEED=1\\;SEEDLEN=29\\;IVAL=L,0.0169183264663712,3.75762168662522\" --overhang --trim5 6",
	  reads  => [ "CTTTGCACCCCTCCCTTGTCGGCTCCCACCCATCCCCATCCGTTGTCCCCGCCCCCGCCCGCCGGTCGTCACTCCCCGTTTGCGTCATGCCCCTCACCCTCCCTTTGTCGGCTCGCACCCCTCCCCATCCGTTGTCCCCGCCCCCGCTCTCGGGGTCTTCACGCCCCGCTTGCTTCATGCCCCTCACTCGCACCCCG" ],
	},

	{ name   => "matchesRef regression 3",
	  ref    => [ "GAAGNTTTTCCAATATTTTTAATTTCCTCTATTTTTCTCTCGTCTTGNTCTAC" ],
	  #
	  #       NNNNNGA------A-------------------G-NTTT
	  #            ||||||||||||||||||||||||||||||||||
	  #       CCAAT-ATTTTTAATTTCCTCTATTTTTCTCTCGTCTTG
	  args   => "--policy \"MMP=R\\;MIN=L,8.8,-8.1\" --overhang",
	  reads  => [ "CAAGACGAGAGAAAAATAGAGGAAATTAAAAATATTGG" ],
	},

	{ name   => "matchesRef regression 2",
	  ref    => ["GTTGTCGGCAGCTCTGGATATGTGNTCTCGGGTTTATNTCGTTGTCG",
	             "CCTTGTTNTTAATGCTGCCTGGTTTNG"],
	  args   =>  "--policy \"RDG=2.02030755427021,2.81949533273331\\;MIN=L,-6.52134769703939,-3.39889659588514\\;IVAL=L,0.127835912101927\" --overhang --trim5 5",
	  mate1s => ["TCTGGCGGTTGCGAAGGCCCCTGGCGGTTGCTATGTCCTCTGGCGGTTGCGTTGTCGGCAGCTCG"],
	  mate2s => ["AGAACACATATCCAGAGCTGCCGACAACGAAATGAACCCGAGAGCACAAATCCAGAG"] },

	# Regression test for an issue observed once
	{ name   => "matchesRef regression 1",
	  #            0         1         2         3         4         5         6         7
	  #            01234567890123456789012345678901234567890123456789012345678901234567890
	  ref    => [ "AGGTCGACCGAAAGGCCTAGAGGTCGACCGACAATCTGACCATGGGGCGAGGAGCGAGTAC" ],
	  #                       ||||||||||||||||||||||||||||||||||||||||||||||||||
	  reads  => [            "AAGGCCTAGAGGTCGACCGACAATCTGACCATGGGGCGAGGAGCGAGTACTGGTCTGGGG" ],
	  #                       012345678901234567890123456789012345678901234567890123456789
	  #                       0         1         2         3         4         5
	  args   => "--overhang" },

	# 1 discordant alignment and one concordant alignment.  Discordant because
	# the fragment is too long.

	{ name => "Discordant with different chromosomes",
	  ref    => [ "TTTATAAAAATATTTCCCCCCCC",
										 "CCCCCCTGTCGCTACCGCCCCCCCCCCC" ],
	#                 ATAAAAATAT                 GTCGCTACCG
	#                 ATAAAAATAT                TGTCGCTACC
	#              01234567890123456789012
	#              0         1         2
	#                                     0123456789012345678901234567
	#                                     0         1         2
	  mate1s    => [ "ATAAAAATAT", "ATAAAAATAT" ],
	  mate2s    => [ "GTCGCTACCG", "TGTCGCTACC" ],
	  mate1fw   => 1,
	  mate2fw   => 1,
	  args      =>   "-I 0 -X 35",
	  # Not really any way to flag an alignment as discordant
	  pairhits  => [ { "3,7" => 1 }, { "3,6" => 1 } ],
	  rnext_map => [ { 3 => 1, 7 => 0 }, { 3 => 1, 6 => 0 } ],
	  pnext_map => [ { 3 => 7, 7 => 3 }, { 3 => 6, 6 => 3 } ] },

	# Paired-end reads that should align
	#{ name     => "Fastq paired 4",
	#  ref      => [     "AGCATCGATCAAAAACTGA" ],
	#  args     => "-s 1 -L 4 -i C,1,0",
	#  #                  AGCATCGATC
	#  #                          TCAAAAACTGA
	#  #                  0123456789012345678
	#  fastq1  => "\n\n\r\n\@r0\nAGCATCGATC\r\n+\n\nIIIIIIIIII\n\n".
	#             #"\n\n\@r1\nTC\r\n+\n\nII\n\n".
	#             "\n\n\@r2\nTCAGTTTTTGA\r\n+\n\nIIIIIIIIIII\n\n",
	#  fastq2  => "\n\n\r\n\@r0\nTCAGTTTTTGA\n+\n\nIIIIIIIIIII\n\n".
	#             #"\n\n\r\n\@r1\nAG\r\n+\nII".
	#             "\n\@r2\nAGCATCGATC\r\n+\nIIIIIIIIII",
	#  paired   => 1,
	#  pairhits =>    [ { }, { "*,*" => 1 }, { "0,8" => 1 } ],
	#  pairhits =>    [ { "0,8" => 1 } ],
	#  samoptflags_map => [
	#  { },
	#  { "*" => { "YT:Z:UP" => 1, "YF:Z:LN"  => 1 } },
	##  { 0   => { "MD:Z:10" => 1, "YT:Z:CP" => 1 },
	#	8   => { "MD:Z:11" => 1, "YT:Z:CP" => 1 } }]
	#},

	#{ name     => "Tabbed paired 4",
	#  ref      => [     "AGCATCGATCAAAAACTGA" ],
	#  args     => "-s 1 -L 4 -i C,1,0",
	#  #                  AGCATCGATC
	#  #                          TCAAAAACTGA
	#  #                  0123456789012345678
	#  tabbed   => "\n\n\r\nr0\tAGCATCGATC\tIIIIIIIIII\tTCAGTTTTTGA\tIIIIIIIIIII\n\n".
	#              "\n\nr1\tTC\tII\tAG\tII".
	#              "\n\nr2\tTCAGTTTTTGA\tIIIIIIIIIII\tAGCATCGATC\tIIIIIIIIII\n\n",
	#  paired   => 1,
	#  #pairhits =>    [ { }, { "*,*" => 1 }, { "0,8" => 1 } ],
	#  pairhits =>    [ { }, { "0,8" => 1 } ],
	#  samoptflags_map => [
	#  { },
	#  #{ "*" => { "YT:Z:UP" => 1, "YF:Z:LN"  => 1 } },
	#  { 0   => { "MD:Z:10" => 1, "YT:Z:CP" => 1 },
#		8   => { "MD:Z:11" => 1, "YT:Z:CP" => 1 } }]
	#},

	#{ name     => "Fasta paired 4",
	#  ref      => [     "AGCATCGATCAAAAACTGA" ],
	#  args     => "-s 1 -L 4 -i C,1,0",
	#  #                  AGCATCGATC
	#  #                          TCAAAAACTGA
	#  #                  0123456789012345678
	#  fasta1  => "\n\n\r\n>r0\nAGCATCGATC\r\n".
	#  #           "\n\n>r1\nTC\r\n".
	#             "\n\n>r2\nTCAGTTTTTGA\r\n",
	#  fasta2  => "\n\n\r\n>r0\nTCAGTTTTTGA\n\n".
	#  #           "\n\n\r\n>r1\nAG".
	#             "\n>r2\nAGCATCGATC",
	# # pairhits =>    [ { }, { "*,*" => 1 }, { "0,8" => 1 } ],
	#  pairhits =>    [ { }, { "0,8" => 1 } ],
	#  samoptflags_map => [
	#  { },
	#  #{ "*" => { "YT:Z:UP" => 1, "YF:Z:LN"  => 1 } },
	#  { 0   => { "MD:Z:10" => 1, "YT:Z:CP" => 1 },
	#	8   => { "MD:Z:11" => 1, "YT:Z:CP" => 1 } }]
	#},

	#{ name     => "Raw paired 4",
	#  ref      => [     "AGCATCGATCAAAAACTGA" ],
	#  args     => "-s 1 -L 4 -i C,1,0",
	#  #                  AGCATCGATC
	#  #                          TCAAAAACTGA
	#  #                  0123456789012345678
	#  raw1    => "\n\n\r\nAGCATCGATC\r\n".
	##             "\n\nTC\r\n".
	#             "\n\nTCAGTTTTTGA\r\n",
	#  raw2    => "\n\n\r\nTCAGTTTTTGA\n\n".
	#             "\n\n\r\nAG".
	#             "\nAGCATCGATC",
	#  pairhits =>    [ { }, { "*,*" => 1 }, { "0,8" => 1 } ],
	#  pairhits =>    [ { }, { "0,8" => 1 } ],
	#  samoptflags_map => [
	#  { },
	#  { "*" => { "YT:Z:UP" => 1, "YF:Z:LN"  => 1 } },
	#  { 0   => { "MD:Z:10" => 1, "YT:Z:CP" => 1 },
#		8   => { "MD:Z:11" => 1, "YT:Z:CP" => 1 } }]
	#},

	#
	# Check that skipping of empty reads is handled correctly.  A read that is
	# empty or becomes empty after --trim3/--trim5 are applied should still
	# count as a first-class read that gets propagated up into the alignment
	# loop.  And it should be counted in the -s/-u totals.
	#

	{ ref      => [     "AGCATCGATCAGTATCTGA" ],
	  reads    => [ "",    "ATCGATCAGTA" ],
	  args     => "-s 1",
	  hits     => [ {}, { 3 => 1 }] },

	{ ref      => [     "AGCATCGATCAGTATCTGA" ],
	  mate1s   => [ "", "AGCATCGATC" ],
	  mate2s   => [ "",          "TCAGATACTG" ],
	  args     => "-s 1",
	  pairhits => [ {}, { "0,9" => 1 }] },

	{ ref      => [     "AGCATCGATCAGTATCTGA" ],
	  reads    => [ "",    "ATCGATCAGTA" ],
	  args     => "-s 2",
	  hits     => [ {}, {} ] },

	{ ref      => [     "AGCATCGATCAGTATCTGA" ],
	  mate1s   => [ "", "AGCATCGATC" ],
	  mate2s   => [ "",          "TCAGATACTG" ],
	  args     => "-s 2",
	  pairhits => [ {}, {} ] },

	{ ref    => [     "AGCATCGATCAGTATCTGA" ],
	  reads  => [ "",    "ATCGATCAGTA", "AGTATCTGA" ],
	  args   => "-s 1 -u 1",
	  hits   => [ {}, { 3 => 1 }] },

	{ ref    => [     "AGCATCGATCAGTATCTGA" ],
	  reads  => [ "AC",  "ATCGATCAGTA" ],
	  args   => "-s 1 --trim3 2",
	  norc   => 1,
	  hits   => [ {}, { 3 => 1 }] },

	{ ref    => [     "AGCATCGATCAGTATCTGA" ],
	  reads  => [ "AC",  "ATCGATCAGTA" ],
	  args   => "-s 1 --trim3 2",
	  nofw   => 1,
	  hits   => [ {}, { 5 => 1 }] },

	{ ref    => [     "AGCATCGATCAGTATCTGA" ],
	  reads  => [ "AC",  "ATCGATCAGTA" ],
	  args   => "-s 1 --trim5 2",
	  nofw   => 1,
	  hits   => [ {}, { 3 => 1 }] },

	{ ref    => [     "AGCATCGATCAGTATCTGA" ],
	  reads  => [ "AC",  "ATCGATCAGTA" ],
	  args   => "-s 1 --trim5 2",
	  norc   => 1,
	  hits   => [ {}, { 5 => 1 }] },

	#
	# Alignment with overhang
	#

	{ ref    => [ "TGC" ],
	  reads  => [ "ATGC" ],
	  args   => "--overhang --policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,1,0\"",
	  hits   => [ { 0 => 1 } ],
	  cigar  => [ "1S3M" ],
	  samoptflags => [
		{ "AS:i:-1" => 1, "YT:Z:UU" => 1, "MD:Z:3" => 1, "XN:i:1" => 1 } ]
	},

	{ ref    => [ "TTGTTCGT" ],
	  reads  => [ "TTGTTCG" ],
	  args   => "--policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,2,0\"",
	  hits   => [ { 0 => 1 } ],
	  cigar  => [ "7M" ],
	  samoptflags => [ { "AS:i:0" => 1, "YT:Z:UU" => 1, "MD:Z:7" => 1 } ]
	},

	{ ref    => [ "TTGTTCGT" ],
	  reads  => [ "TTGTTCG" ],
	  args   => "",
	  hits   => [ { 0 => 1 } ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:7=" ],
	  cigar  => [ "7M" ],
	  samoptflags => [ { "AS:i:0" => 1, "YT:Z:UU" => 1, "MD:Z:7" => 1 } ]
	},

	{ ref    => [ "TTGTTCGT" ],
	  reads  => [  "TGTTCGT", "TTGTTCG" ],
	  args   => "--overhang",
	  hits   => [ { 1 => 1 }, { 0 => 1 } ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:7=", "XM:0,XP:0,XT:UU,XC:7=" ],
	  cigar  => [ "7M", "7M" ],
	  samoptflags => [
		{ "YT:Z:UU" => 1, "MD:Z:7" => 1 },
		{ "YT:Z:UU" => 1, "MD:Z:7" => 1 }
	  ]
	},

	{ ref    => [ "TTGTTCGT" ],
	  reads  => [ "TGTTCGT", "TTGTTCG" ],
	  args   => "",
	  hits   => [ { 1 => 1 }, { 0 => 1 } ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:7=", "XM:0,XP:0,XT:UU,XC:7=" ],
	  cigar  => [ "7M", "7M" ],
	  samoptflags => [
		{ "YT:Z:UU" => 1, "MD:Z:7" => 1 },
		{ "YT:Z:UU" => 1, "MD:Z:7" => 1 }
	  ]
	},

	# Reads 1 and 2 don't have overhang, reads 3 and 4 overhang opposite ends
	{ ref    => [ "TTGTTCGT" ],
	#              TGTTCGT
	#                GTTCGTA
	#             ATTGTTC
	  reads  => [ "TGTTCGT", "GTTCGTA", "ATTGTTC" ],
	  args   => "--overhang --policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,2,0\"",
	  hits   => [ { 1 => 1 }, { 2 => 1 }, { 0 => 1 } ],
	  cigar  => [ "7M", "6M1S", "1S6M" ],
	  samoptflags => [
		{ "YT:Z:UU" => 1, "MD:Z:7" => 1 },
		{ "AS:i:-1" => 1, "XN:i:1" => 1, "YT:Z:UU" => 1, "MD:Z:6" => 1 },
		{ "AS:i:-1" => 1, "XN:i:1" => 1, "YT:Z:UU" => 1, "MD:Z:6" => 1 }
	  ]},

	# Same as previous case but --overhang not specified
	{ ref    => [ "TTGTTCGT" ],
	  reads  => [ "TGTTCGT", "TTGTTCG", "GTTCGTA", "ATTGTTC" ],
	  args   => "--policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,2,0\"",
	  hits   => [ { 1 => 1 }, { 0 => 1 } ], # only the internal hits
	  cigar  => [ "7M", "7M", "*", "*" ],
	  samoptflags => [
		{ "YT:Z:UU" => 1, "MD:Z:7" => 1 },
		{ "YT:Z:UU" => 1, "MD:Z:7" => 1 },
		{ "YT:Z:UU" => 1 },
		{ "YT:Z:UU" => 1 }
	  ]
	},

	# A simple case that should align with or without overhang, with or without
	# a special NCEIL setting.
	{ ref    => [ "TTGTTCGT" ],
	  reads  => [ "TTGTTCG" ],
	  args   => "--overhang --policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,2,0\"",
	  hits   => [ { 0 => 1 } ]},

	{ ref    => [ "TTGTTCGT" ],
	  reads  => [ "TTGTTCG" ],
	  args   => "--overhang",
	  hits   => [ { 0 => 1 } ]},

	#
	# Testing the various -M/-m/-k/-a alignment modes in both unpaired and
	# paired-end modes.  Ensuring that SAM optional flags such as YM:i, YP:i
	# are set properly in all cases.
	#

	#
	# Paired-end
	#

	{ name   => "P.M.58.G.b Unpaired -M 5 w/ 8 hits global, but mate #1 has just 1",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2                                             0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               0123456789012345678901234567                                      0123456789012345678901234567                                                                                                                                               0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGT" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3                                                                                                   4                                                                                                   5                                                                                                   6                                                                                                   7                                                                                                   8                                                                                                   9
	  #            0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  args     => "-X 1000",
	  report   => "-M 5",
	  pairhits   => [{ "12,78"  => 1, "12,249" => 1, "12,315" => 1,
	                   "12,486" => 1, "12,552" => 1, "12,723" => 1,
					   "12,789" => 1, "12,960" => 1 }],
	  hits_are_superset => [ 1 ],
	  cigar_map => [{
		12   => "33M",   78 => "28M",
		249  => "28M",  315 => "28M",
		486  => "28M",  552 => "28M",
		723  => "28M",  789 => "28M",
		960  => "28M"
	  }],
	  samoptflags_map => [ {
	    12 => {   "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:0" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    78 => {   "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    249 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    315 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    486 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    552 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    723 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    789 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    960 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	} ] },

	{ name   => "P.M.58.L.b Unpaired -M 5 w/ 8 hits local, but mate #1 has just 1",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2                                             0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               0123456789012345678901234567                                      0123456789012345678901234567                                                                                                                                               0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGT" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3                                                                                                   4                                                                                                   5                                                                                                   6                                                                                                   7                                                                                                   8                                                                                                   9
	  #            0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  args   =>  "-X 1000 --local",
	  report =>  "-M 5",
	  pairhits   => [{ "12,78"  => 1, "12,249" => 1, "12,315" => 1,
	                   "12,486" => 1, "12,552" => 1, "12,723" => 1,
					   "12,789" => 1, "12,960" => 1 }],
	  hits_are_superset => [ 1 ],
	  cigar_map => [{
		12   => "33M",   78 => "28M",
		249  => "28M",  315 => "28M",
		486  => "28M",  552 => "28M",
		723  => "28M",  789 => "28M",
		960  => "28M"
	  }],
	  samoptflags_map => [ {
	    12 => {   "AS:i:66" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:0" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:56"  => 1 },
	    78 => {   "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    249 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    315 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    486 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    552 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    723 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    789 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    960 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	} ] },

	{ name   => "P.k.58.G.b Unpaired -k 5 w/ 8 hits global, but mate #1 has just 1",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2                                             0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               0123456789012345678901234567                                      0123456789012345678901234567                                                                                                                                               0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGT" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3                                                                                                   4                                                                                                   5                                                                                                   6                                                                                                   7                                                                                                   8                                                                                                   9
	  #            0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  args   =>   "-X 1000",
	  report =>   "-k 5",
	  pairhits   => [{ "12,78"  => 1, "12,249" => 1, "12,315" => 1,
	                   "12,486" => 1, "12,552" => 1, "12,723" => 1,
					   "12,789" => 1, "12,960" => 1 }],
	  hits_are_superset => [ 1 ],
	  cigar_map => [{
		12   => "33M",   78 => "28M",
		249  => "28M",  315 => "28M",
		486  => "28M",  552 => "28M",
		723  => "28M",  789 => "28M",
		960  => "28M"
	  }],
	  samoptflags_map => [ {
	    12 => {   "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:0" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    78 => {   "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    249 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    315 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    486 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    552 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    723 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    789 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    960 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	} ] },

	{ name   => "P.k.58.L.b Unpaired -k 5 w/ 8 hits local, but mate #1 has just 1",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2                                             0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               0123456789012345678901234567                                      0123456789012345678901234567                                                                                                                                               0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG                                      ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACACACACCCCTATAGCTCGGAGCTGACTGGATCGACGACGT" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3                                                                                                   4                                                                                                   5                                                                                                   6                                                                                                   7                                                                                                   8                                                                                                   9
	  #            0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  args   =>   "-X 1000 --local",
	  report =>   "-k 5",
	  pairhits   => [{ "12,78"  => 1, "12,249" => 1, "12,315" => 1,
	                   "12,486" => 1, "12,552" => 1, "12,723" => 1,
					   "12,789" => 1, "12,960" => 1 }],
	  hits_are_superset => [ 1 ],
	  cigar_map => [{
		12   => "33M",   78 => "28M",
		249  => "28M",  315 => "28M",
		486  => "28M",  552 => "28M",
		723  => "28M",  789 => "28M",
		960  => "28M"
	  }],
	  samoptflags_map => [ {
	    12 => {   "AS:i:66" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:0" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:56"  => 1 },
	    78 => {   "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    249 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    315 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    486 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    552 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    723 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    789 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    960 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	} ] },

	{ name   => "P.M.22.G. Paired -M 2 w/ 2 paired hit, 2 unpaired hits each, global",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2         3                                   0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               012345678901234567890123456789012                                 0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGTATCGA" ],
	  #            012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  mate1fw => 1, mate2fw => 0,
	  args    => "-X 150",
	  report  => "-M 2",
	  pairhits  => [ { "12,78" => 1, "249,315" => 1 } ],
	  cigar_map => [{
		12 => "33M", 249 => "33M",
		78 => "28M", 315 => "28M"
	  }],
	  hits_are_superset => [ 1 ],
	  samoptflags_map => [{
		12  => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		         "YM:i:0" => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
		78  => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		         "YM:i:0" => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
		249 => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		         "YM:i:0" => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
		315 => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		         "YM:i:0" => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
	  }]
	},

	{ name   => "P.M.22.L. Paired -M 2 w/ 2 paired hit, 2 unpaired hits each, local",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2         3                                   0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               012345678901234567890123456789012                                 0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGTATCGA" ],
	  #            012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  mate1fw => 1, mate2fw => 0,
	  args    => "--local -X 150",
	  report  => "-M 2",
	  pairhits  => [ { "12,78" => 1, "249,315" => 1 } ],
	  cigar_map => [{
		12 => "33M", 249 => "33M",
		78 => "28M", 315 => "28M"
	  }],
	  hits_are_superset => [ 1 ],
	  samoptflags_map => [{
		12  => { "AS:i:66" => 1, "XS:i:66" => 1, "MD:Z:33" => 1,
		         "YM:i:0"  => 1, "YP:i:0"  => 1, "YT:Z:CP" => 1, "YS:i:56" => 1 },
		78  => { "AS:i:56" => 1, "XS:i:56" => 1, "MD:Z:28" => 1,
		         "YM:i:0"  => 1, "YP:i:0"  => 1, "YT:Z:CP" => 1, "YS:i:66" => 1 },
		249 => { "AS:i:66" => 1, "XS:i:66" => 1, "MD:Z:33" => 1,
		         "YM:i:0"  => 1, "YP:i:0"  => 1, "YT:Z:CP" => 1, "YS:i:56" => 1 },
		315 => { "AS:i:56" => 1, "XS:i:56" => 1, "MD:Z:28" => 1,
		         "YM:i:0"  => 1, "YP:i:0"  => 1, "YT:Z:CP" => 1, "YS:i:66" => 1 },
	  }]
	},

	{ name   => "P.k.2.G. Paired -k 1 w/ 2 paired hit, 2 unpaired hits each, global",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2         3                                   0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               012345678901234567890123456789012                                 0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGTATCGA" ],
	  #            012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  mate1fw => 1, mate2fw => 0,
	  args    => "-X 150",
	  report  => "-k 1",
	  pairhits  => [ { "12,78" => 1, "249,315" => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar_map => [{
		12 => "33M", 249 => "33M",
		78 => "28M", 315 => "28M"
	  }],
	  samoptflags_map => [{
		12  => { "AS:i:0"  => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		         "YT:Z:CP" => 1, "YS:i:0" => 1 },
		78  => { "AS:i:0"  => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		         "YT:Z:CP" => 1, "YS:i:0" => 1 },
		249 => { "AS:i:0"  => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		         "YT:Z:CP" => 1, "YS:i:0" => 1 },
		315 => { "AS:i:0"  => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		         "YT:Z:CP" => 1, "YS:i:0" => 1 },
	  }]
	},

	{ name   => "P.k.2.L. Paired -k 1 w/ 2 paired hit, 2 unpaired hits each, local",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2         3                                   0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               012345678901234567890123456789012                                 0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGTATCGA" ],
	  #            012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  mate1fw => 1, mate2fw => 0,
	  args    => "--local -X 150",
	  report  => "-k 1",
	  pairhits  => [ { "12,78" => 1, "249,315" => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar_map => [{
		12 => "33M", 249 => "33M",
		78 => "28M", 315 => "28M"
	  }],
	  samoptflags_map => [{
		12  => { "AS:i:66" => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		         "YT:Z:CP" => 1, "YS:i:56" => 1 },
		78  => { "AS:i:56" => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		         "YT:Z:CP" => 1, "YS:i:66" => 1 },
		249 => { "AS:i:66" => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		         "YT:Z:CP" => 1, "YS:i:56" => 1 },
		315 => { "AS:i:56" => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		         "YT:Z:CP" => 1, "YS:i:66" => 1 },
	  }]
	},

	{ name   => "P.M.2.G. Paired -M 1 w/ 2 paired hit, 2 unpaired hits each, global",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2         3                                   0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               012345678901234567890123456789012                                 0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGTATCGA" ],
	  #            012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  mate1fw => 1, mate2fw => 0,
	  report  =>   "-M 1 -X 150",
	  pairhits  => [ { "12,78" => 1, "249,315" => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar_map => [{
		12 => "33M", 249 => "33M",
		78 => "28M", 315 => "28M"
	  }],
	  samoptflags_map => [{
		12  => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		         "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
		78  => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		         "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
		249 => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		         "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
		315 => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		         "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
	  }]
	},

	{ name   => "P.M.2.L. Paired -M 1 w/ 2 paired hit, 2 unpaired hits each, local",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2         3                                   0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               012345678901234567890123456789012                                 0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGTATCGA" ],
	  #            012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  mate1fw => 1, mate2fw => 0,
	  report  =>   "-M 1 --local -X 150",
	  pairhits  => [ { "12,78" => 1, "249,315" => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar_map => [{
		12 => "33M", 249 => "33M",
		78 => "28M", 315 => "28M"
	  }],
	  samoptflags_map => [{
		12  => { "AS:i:66" => 1, "XS:i:66" => 1, "MD:Z:33" => 1,
		         "YM:i:1"  => 1, "YP:i:1"  => 1, "YT:Z:CP" => 1, "YS:i:56" => 1 },
		78  => { "AS:i:56" => 1, "XS:i:56" => 1, "MD:Z:28" => 1,
		         "YM:i:1"  => 1, "YP:i:1"  => 1, "YT:Z:CP" => 1, "YS:i:66" => 1 },
		249 => { "AS:i:66" => 1, "XS:i:66" => 1, "MD:Z:33" => 1,
		         "YM:i:1"  => 1, "YP:i:1"  => 1, "YT:Z:CP" => 1, "YS:i:56" => 1 },
		315 => { "AS:i:56" => 1, "XS:i:56" => 1, "MD:Z:28" => 1,
		         "YM:i:1"  => 1, "YP:i:1"  => 1, "YT:Z:CP" => 1, "YS:i:66" => 1 },
	  }]
	},

	{ name   => "P.k.1.G. Paired -k w/ 1 paired hit, 1 unpaired hit each, global",
	  #                        0         1         2         3                                   0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGTATCGA" ],
	  #            012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  mate1fw => 1,  mate2fw => 0,
	  report  =>   "-k 1 -X 150",
	  pairhits  => [ { "12,78" => 1 } ],
	  cigar_map => [{
		12 => "33M",
		78 => "28M"
	  }],
	  samoptflags_map => [{
		12 => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		        "YM:i:0" => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
		78 => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		        "YM:i:0" => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
	  }]
	},

	{ name   => "P.k.1.L. Paired -k 1 w/ 1 paired hit, 1 unpaired hit each, local",
	  #                        0         1         2         3                                   0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGTATCGA" ],
	  #            012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  mate1fw => 1,  mate2fw => 0,
	  args    => "--local -X 150",
	  report  => "-k 1",
	  pairhits  => [ { "12,78" => 1 } ],
	  cigar_map => [{
		12 => "33M",
		78 => "28M"
	  }],
	  samoptflags_map => [{
		12 => { "AS:i:66" => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		        "YM:i:0"  => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:56" => 1 },
		78 => { "AS:i:56" => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		        "YM:i:0"  => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:66" => 1 },
	  }]
	},

	{ name   => "P.M.1.G. Paired -M w/ 1 paired hit, 1 unpaired hit each, global",
	  #                        0         1         2         3                                   0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGTATCGA" ],
	  #            012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  mate1fw => 1,  mate2fw => 0,
	  args    =>   "-X 150",
	  report  =>   "-M 1",
	  pairhits  => [ { "12,78" => 1 } ],
	  cigar_map => [{
		12 => "33M",
		78 => "28M"
	  }],
	  samoptflags_map => [{
		12 => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		        "YM:i:0" => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
		78 => { "AS:i:0" => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		        "YM:i:0" => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:0" => 1 },
	  }]
	},

	{ name   => "P.M.1.L. Paired -M w/ 1 paired hit, 1 unpaired hit each, local",
	  #                          0         1         2         3                                   0         1         2
	  #                          012345678901234567890123456789012                                 0123456789012345678901234567
	  #                          CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref      => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGTATCGA" ],
	  #              012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345
	  #              0         1         2         3         4         5         6         7         8         9         0         1         2
	  mate1s   => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s   => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  mate1fw  => 1,  mate2fw => 0,
	  args     =>   "-X 150 --local",
	  report   =>   "-M 1",
	  pairhits => [ { "12,78" => 1 } ],
	  cigar_map => [{
		12 => "33M",
		78 => "28M"
	  }],
	  samoptflags_map => [{
		12 => { "AS:i:66" => 1, "XS:i:0" => 1, "MD:Z:33" => 1,
		        "YM:i:0"  => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:56" => 1 },
		78 => { "AS:i:56" => 1, "XS:i:0" => 1, "MD:Z:28" => 1,
		        "YM:i:0"  => 1, "YP:i:0" => 1, "YT:Z:CP" => 1, "YS:i:66" => 1 },
	  }]
	},

	{ name   => "P.M.58.G. Unpaired -M 5 w/ 8 hits global",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2         3                                   0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               012345678901234567890123456789012                                 0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGA" ],
	  #            012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3                                                                                                   4                                                                                                   5                                                                                                   6                                                                                                   7                                                                                                   8                                                                                                   9                                                                                                   0                                                                                                   1                                                                                                   2                                                                                                   3                                                                                                   4                                                                                                   5                                                                                                   6                                                                                                   7                                                                                                   8                                                                                                   9                                                                                                   0                                                                                                   1                                                                                                   2                                                                                                   3                                                                                                   4                                                                                                   5                                                                                                   6
	  #            0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   2                                                                                                   2                                                                                                   2                                                                                                   2                                                                                                   2                                                                                                   2                                                                                                   2
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  args   =>   "-X 150",
	  report =>   "-M 5",
	  pairhits  => [ { "12,78"     => 1, "249,315"   => 1, "486,552"   => 1,
	                   "723,789"   => 1, "960,1026"  => 1, "1197,1263" => 1,
					   "1434,1500" => 1, "1671,1737" => 1, "1908,1974" => 1,
					   "2145,2211" => 1, "2382,2448" => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar_map => [{
		12   => "33M",   78 => "28M",
		249  => "33M",  315 => "28M",
		486  => "33M",  552 => "28M",
		723  => "33M",  789 => "28M",
		960  => "33M", 1026 => "28M",
		1197 => "33M", 1263 => "28M",
		1434 => "33M", 1500 => "28M",
		1671 => "33M", 1737 => "28M",
		1908 => "33M", 1974 => "28M",
		2145 => "33M", 2211 => "28M",
		2382 => "33M", 2448 => "28M",
	  }],
	  samoptflags_map => [ {
	    12 => {   "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    78 => {   "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    249 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    315 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    486 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    552 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    723 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    789 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    960 => {  "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    1026 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    1197 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    1263 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    1434 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    1500 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    1671 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    1737 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    1908 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    1974 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    2145 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    2211 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    2382 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	    2448 => { "AS:i:0" => 1, "XS:i:0" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:0"  => 1 },
	} ] },

	{ name   => "P.M.58.L. Unpaired -M 5 w/ 8 hits local",
	  #                        0         1         2         3                                   0         1         2                                                                                                                                                      0         1         2         3                                   0         1         2
	  #                        012345678901234567890123456789012                                 0123456789012345678901234567                                                                                                                                               012345678901234567890123456789012                                 0123456789012345678901234567
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG                                                                                                                                               CAGCGTACGGTATCTAGCTATGGGCATCGATCG                                 ACACACCCCTATAGCTCGGAGCTGACTG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGAGCGGTATCTACAGCCACTCATCACACACCCCTATAGCTCGGAGCTGACTGGGTTACTGGGGGGGATGCGTATCGACTATCGACAATATGACGCGTCGGTCACCCCATAATATGCAAAAATTATAGCTCACGACGCGTACTAATAGAAAACGCGCTATCAGCCTCCGACGCGGCGGTATCGAAGACGCAGTC" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3                                                                                                   4                                                                                                   5                                                                                                   6                                                                                                   7                                                                                                   8                                                                                                   9                                                                                                   0                                                                                                   1                                                                                                   2                                                                                                   3                                                                                                   4                                                                                                   5                                                                                                   6                                                                                                   7                                                                                                   8                                                                                                   9
	  #            0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   0                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1                                                                                                   1
	  mate1s => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  mate2s => [ "CAGTCAGCTCCGAGCTATAGGGGTGTGT" ], # rev comped
	  args   =>   "--local -X 150",
	  report =>   "-M 5",
	  pairhits  => [ { "12,78"     => 1, "249,315"   => 1, "486,552"   => 1,
	                   "723,789"   => 1, "960,1026"  => 1, "1197,1263" => 1,
					   "1434,1500" => 1, "1671,1737" => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar_map => [{
		12   => "33M",   78 => "28M",
		249  => "33M",  315 => "28M",
		486  => "33M",  552 => "28M",
		723  => "33M",  789 => "28M",
		960  => "33M", 1026 => "28M",
		1197 => "33M", 1263 => "28M",
		1434 => "33M", 1500 => "28M",
		1671 => "33M", 1737 => "28M"
	  }],
	  samoptflags_map => [ {
	    12 => {   "AS:i:66" => 1, "XS:i:66" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:56"  => 1 },
	    78 => {   "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    249 => {  "AS:i:66" => 1, "XS:i:66" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:56"  => 1 },
	    315 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    486 => {  "AS:i:66" => 1, "XS:i:66" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:56"  => 1 },
	    552 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    723 => {  "AS:i:66" => 1, "XS:i:66" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:56"  => 1 },
	    789 => {  "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    960 => {  "AS:i:66" => 1, "XS:i:66" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:56"  => 1 },
	    1026 => { "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    1197 => { "AS:i:66" => 1, "XS:i:66" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:56"  => 1 },
	    1263 => { "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    1434 => { "AS:i:66" => 1, "XS:i:66" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:56"  => 1 },
	    1500 => { "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	    1671 => { "AS:i:66" => 1, "XS:i:66" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:33" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:56"  => 1 },
	    1737 => { "AS:i:56" => 1, "XS:i:56" => 1, "XN:i:0"   => 1, "XM:i:0"  => 1,
		          "XO:i:0" => 1, "XG:i:0" => 1, "NM:i:0"   => 1, "MD:Z:28" => 1,
		          "YM:i:1" => 1, "YP:i:1" => 1, "YT:Z:CP"  => 1, "YS:i:66"  => 1 },
	} ] },

	#
	# Unpaired
	#

	{ name   => "U.M.1.G. Unpaired -M w/ 1 hit global",
	  #                        0         1         2         3
	  #                        012345678901234567890123456789012
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG
	  #                        CAGCGTACGGTATCTAGCTATG
	  #                                GGTATCTAGCTATGGGCATCGA
	  #            AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGA
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGA" ],
	  #            01234567890123456789012345678901234567890123456789012345
	  #            0         1         2         3         4         5
	  reads  => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  report =>   "-M 1",
	  hits   => [ { 12 => 1 } ],
	  cigar  => [ "33M" ],
	  samoptflags => [{
		"AS:i:0"   => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:33"  => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	}]
	},

	{ name   => "U.M.1.L. Unpaired -M w/ 1 hit local",
	  #                        0         1         2         3
	  #                        012345678901234567890123456789012
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGA" ],
	  #            01234567890123456789012345678901234567890123456789012345
	  #            0         1         2         3         4         5
	  reads  => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  args   =>   "--local",
	  report =>   "-M 1",
	  hits   => [ { 12 => 1 } ],
	  cigar  => [ "33M" ],
	  samoptflags => [{
		"AS:i:66"  => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:33"  => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	}]
	},

	{ name   => "U.k.1.G. Unpaired -k 1 w/ 1 hit global",
	  #                        0         1         2         3
	  #                        012345678901234567890123456789012
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGA" ],
	  #            01234567890123456789012345678901234567890123456789012345
	  #            0         1         2         3         4         5
	  reads  => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  report =>   "-k 1",
	  hits   => [ { 12 => 1 } ],
	  cigar  => [ "33M" ],
	  samoptflags => [ {
		"AS:i:0"   => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:33"  => 1, # mismatching positions/bases
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.M.1.L. Unpaired -m w/ 1 hit local",
	  #                        0         1         2         3
	  #                        012345678901234567890123456789012
	  #                        CAGCGTACGGTATCTAGCTATGGGCATCGATCG
	  ref    => [ "AGACGCAGTCACCAGCGTACGGTATCTAGCTATGGGCATCGATCGACGACGTACGA" ],
	  #            01234567890123456789012345678901234567890123456789012345
	  #            0         1         2         3         4         5
	  reads  => [ "CAGCGTACGGTATCTAGCTATGGGCATCGATCG" ],
	  args   => "--local",
	  report => "-k 1",
	  hits   => [ { 12 => 1 } ],
	  cigar  => [ "33M" ],
	  samoptflags => [ {
		"AS:i:66"  => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:33"  => 1, # mismatching positions/bases
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.M.2.G. Unpaired -M 1 w/ 2 hit global",
	  #                  0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234
	  #            0         1         2         3         4         5         6         7         8
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  args   =>   "",
	  report =>   "-M 1",
	  hits   => [ { 6 => 1, 48 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "30M" ],
	  samoptflags => [ {
		"AS:i:0"   => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:1"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.M.2.L. Unpaired -M 1 w/ 2 hit local",
	  #                  0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234
	  #            0         1         2         3         4         5         6         7         8
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  args   =>   "--local",
	  report =>   "-M 1",
	  hits   => [ { 6 => 1, 48 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "30M" ],
	  samoptflags => [ {
		"AS:i:60"  => 1, # alignment score
		"XS:i:60"  => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:1"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.k.2.G. Unpaired -k 1 w/ 2 hit global",
	  #                  0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234
	  #            0         1         2         3         4         5         6         7         8
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  report =>   "-k 1",
	  hits   => [ { 6 => 1, 48 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "30M" ],
	  samoptflags => [ {
		"AS:i:0"   => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.k.2.L. Unpaired -k 1 w/ 2 hit local",
	  #                  0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234
	  #            0         1         2         3         4         5         6         7         8
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  report =>   "-k 1",
	  hits   => [ { 6 => 1, 48 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "30M" ],
	  samoptflags => [ {
		"AS:i:0"   => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.M.22.G. Unpaired -M 2 w/ 2 hit global",
	  #                  0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234
	  #            0         1         2         3         4         5         6         7         8
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  report =>   "-M 2",
	  hits   => [ { 6 => 1, 48 => 1 } ],
	  cigar  => [ "30M" ],
	  hits_are_superset => [ 1 ],
	  samoptflags => [ {
		"AS:i:0"   => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.M.22.L. Unpaired -M 2 w/ 2 hit local",
	  #                  0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234
	  #            0         1         2         3         4         5         6         7         8
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  report =>   "-M 2 --local",
	  hits   => [ { 6 => 1, 48 => 1 } ],
	  cigar  => [ "30M" ],
	  hits_are_superset => [ 1 ],
	  samoptflags => [ {
		"AS:i:60"  => 1, # alignment score
		"XS:i:60"  => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.k.22.G. Unpaired -k 2 w/ 2 hit global",
	  #                  0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234
	  #            0         1         2         3         4         5         6         7         8
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  report =>   "-k 2",
	  hits   => [ { 6 => 1, 48 => 1 } ],
	  cigar  => [ "30M" ],
	  samoptflags => [ {
		"AS:i:0"   => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.k.22.L. Unpaired -k 2 w/ 2 hit local",
	  #                  0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234
	  #            0         1         2         3         4         5         6         7         8
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  args   => "--local",
	  report => "-k 2",
	  hits   => [ { 6 => 1, 48 => 1 } ],
	  cigar  => [ "30M" ],
	  samoptflags => [ {
		"AS:i:60"  => 1, # alignment score
		"XS:i:60"  => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.M.58.G. Unpaired -M 5 w/ 8 hits global",
	  #                  0         1         2                     0         1         2                      0         1         2                     0         1         2                      0         1         2                     0         1         2                      0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  args   =>   "-X 150",
	  report =>   "-M 5",
	  hits   => [ { 6 => 1, 48 => 1, 91 => 1, 133 => 1, 176 => 1, 218 => 1, 261 => 1, 303 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "30M" ],
	  samoptflags => [ {
		"AS:i:0"   => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:1"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.M.58.L. Unpaired -M 5 w/ 8 hits global",
	  #                  0         1         2                     0         1         2                      0         1         2                     0         1         2                      0         1         2                     0         1         2                      0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  args   =>   "--local",
	  report =>   "-M 5",
	  hits   => [ { 6 => 1, 48 => 1, 91 => 1, 133 => 1, 176 => 1, 218 => 1, 261 => 1, 303 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "30M" ],
	  samoptflags => [ {
		"AS:i:60"  => 1, # alignment score
		"XS:i:60"  => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:1"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.k.58.G. Unpaired -k 5 w/ 8 hits global",
	  #                  0         1         2                     0         1         2                      0         1         2                     0         1         2                      0         1         2                     0         1         2                      0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  report =>   "-k 5",
	  hits   => [ { 6 => 1, 48 => 1, 91 => 1, 133 => 1, 176 => 1, 218 => 1, 261 => 1, 303 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "30M" ],
	  samoptflags => [ {
		"AS:i:0"   => 1, # alignment score
		"XS:i:0"   => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	{ name   => "U.k.58.L. Unpaired -k 5 w/ 8 hits local",
	  #                  0         1         2                     0         1         2                      0         1         2                     0         1         2                      0         1         2                     0         1         2                      0         1         2                     0         1         2
	  #                  012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789             012345678901234567890123456789            012345678901234567890123456789
	  #                  AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA             AGATTACGGATCTACGATTCGAGTCGGTCA            AGATTACGGATCTACGATTCGAGTCGGTCA
	  ref    => [ "AGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGAAGACGCAGATTACGGATCTACGATTCGAGTCGGTCAGTCACCAGCGTAAGATTACGGATCTACGATTCGAGTCGGTCAAGTGCGA" ],
	  #            0123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789
	  #            0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3         4         5         6         7         8         9         0         1         2         3
	  #            0                                                                                                   1                                                                                                   2                                                                                                   3
	  reads  => [ "AGATTACGGATCTACGATTCGAGTCGGTCA" ],
	  args   => "--local",
	  report => "-k 5",
	  hits   => [ { 6 => 1, 48 => 1, 91 => 1, 133 => 1, 176 => 1, 218 => 1, 261 => 1, 303 => 1 } ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "30M" ],
	  samoptflags => [ {
		"AS:i:60"  => 1, # alignment score
		"XS:i:60"  => 1, # suboptimal alignment score
		"XN:i:0"   => 1, # num ambiguous ref bases
		"XM:i:0"   => 1, # num mismatches
		"XO:i:0"   => 1, # num gap opens
		"XG:i:0"   => 1, # num gap extensions
		"NM:i:0"   => 1, # num edits
		"MD:Z:30"  => 1, # mismatching positions/bases
		"YM:i:0"   => 1, # read aligned repetitively in unpaired fashion
		"YT:Z:UU"  => 1, # unpaired read aligned in unpaired fashion
	} ] },

	# Following cases depend on this being the case:
	#
	# static const float DEFAULT_CEIL_CONST = 3.0f;
	# static const float DEFAULT_CEIL_LINEAR = 3.0f;

	# Just enough budget for hits, so it should align
	{ ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCAT" ], # budget = 3 + 8 * 3 = 27
	  args   => "-L 6 -i C,1,0 --policy \"MMP=C27\\;MIN=L,-3,-3\\;RDG=25,15\\;RFG=25,15\"", # penalty = 27
	  report => "-a",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU,XC:6=1X1=" ],
	  cigar  => [ "8M" ],
	  samoptflags => [ { "AS:i:-27" => 1, "XS:i:-27" => 1, "NM:i:1" => 1,
	                     "XM:i:1" => 1, "YT:Z:UU" => 1, "MD:Z:6G1" => 1 } ] },

	# Not quite enough budget for hits, so it should NOT align
	{ ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCAT" ], # budget = 3 + 8 * 3 = 27
	  args   =>   "-L 6 -i C,1,0 --policy \"MMP=C28\\;MIN=L,-3,-3\\;RDG=25,15\\;RFG=25,15\"", # penalty = 28
	  report =>   "-a",
	  hits   => [ { "*" => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU" ],
	  cigar  => [ "*" ],
	  samoptflags => [ { "YT:Z:UU" => 1 } ] },

	# Check that using a seed of length 1 with 1-mismatch doesn't crash.
	# Perhaps we should disallow it though?

	{ ref    => [ "AAAAAAAAAAAAAAAAAAAAAAAAACCCCCCCCCCCCCCCCCCCCCCCC" ],
	  reads  => [ "AA", "AA", "AA", "AA", "CC", "CC", "CC", "CC", "AA", "AA", "AA", "AA", "CC", "CC", "CC", "CC" ],
	  names  => [ "r1", "r1", "r1", "r1", "r2", "r2", "r2", "r2", "r3", "r3", "r3", "r3", "r4", "r4", "r4", "r4" ],
	  args   => "--policy \"SEED=1\\;SEEDLEN=2\"",
	  check_random => 1,
	  report => "-k 1" },

	#
	# Gap penalties
	#

	# Alignment with 1 read gap
	{ name   => "Gap penalties 1",
	  ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCTTTGTT" ], # budget = 3 + 12 * 3 = 39
	  args   =>   "--policy \"MMP=C30\\;SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;RDG=29,10\\;RFG=25,15\\;MIN=L,-3,-3\"",
	  report =>   "-a",
	  hits   => [ { 0 => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU,XC:6=1D6=" ],
	  cigar  => [ "6M1D6M" ],
	  samoptflags => [{
		"AS:i:-39" => 1, "NM:i:1" => 1, "XO:i:1" => 1, "XG:i:1" => 1,
		"YT:Z:UU" => 1, "MD:Z:6^G6" => 1 }]
	},

	# Alignment with 1 read gap, but not enough budget
	{ name   => "Gap penalties 2",
	  ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCTTTGTT" ], # budget = 3 + 12 * 3 = 39
	  args   =>   "--policy \"MMP=C30\\;SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;RDG=30,10\\;RFG=25,15\\;MIN=L,-3,-3\"",
	  report =>   "-a",
	  hits   => [ { "*" => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU" ],
	  cigar  => [ "*" ],
	  samoptflags => [{ "YT:Z:UU" => 1 }]
	},

	# Alignment with 1 reference gap
	{ name   => "Gap penalties 3",
	  ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCGATTTGTT" ], # budget = 3 + 14 * 3 = 45
	  args   =>   "--policy \"MMP=C30\\;SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;RDG=25,15\\;RFG=30,15\\;MIN=L,-3,-3\"",
	  report =>   "-a",
	  hits   => [ { 0 => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU,XC:7=1I6=" ],
	  cigar  => [ "7M1I6M" ],
	  samoptflags => [{ "AS:i:-45" => 1, "NM:i:1" => 1, "XO:i:1" => 1,
	                    "XG:i:1" => 1, "YT:Z:UU" => 1, "MD:Z:13" => 1 }]
	},

	#      0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5
	#      T T G T T C G T T T G T T C G T
	#       0 1 1 0 2 3 1 0 0 1 1 0 2 3 1
	# 0 T  x
	# 0  0  x
	# 1 T    x
	# 1  1    x
	# 2 G      x
	# 2  1      x
	# 3 T        x
	# 3  0        x
	# 4 T          x
	# 4  2          x
	# 5 C            x
	# 5  3            x
	# 6 G
	# 6  2            x
	# 7 A
	# 7  3              x
	# 8 T                x
	# 8  0                x
	# 9 T                  x
	# 9  0                  x
	# 0 T                    x
	# 0  1                    x
	# 1 G                      x
	# 1  1                      x
	# 2 T                        x
	# 2  0                        x
	# 3 T
	#

	# Alignment with 1 reference gap, but not enough budget
	{ name   => "Gap penalties 4",
	  ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCGATTTGTT" ], # budget = 3 + 14 * 3 = 45
	  args   =>   "--policy \"MMP=C30\\;SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;RDG=25,15\\;RFG=30,16\\;MIN=L,-3,-3\"",
	  report =>   "-a",
	  hits   => [ { "*" => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU" ],
	  cigar  => [ "*" ],
	  samoptflags => [ { "YT:Z:UU" => 1 } ] },

	# Alignment with 1 reference gap, but not enough budget
	{ name   => "Gap penalties 5",
	  ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCGATTTGTT" ], # budget = 3 + 14 * 3 = 45
	  args   =>   "--policy \"MMP=C30\\;SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;RDG=25,15\\;RFG=31,15\\;MIN=L,-3,-3\"",
	  report =>   "-a",
	  hits   => [ { "*" => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU" ],
	  cigar  => [ "*" ],
	  samoptflags => [ { "YT:Z:UU" => 1 } ] },

	# Alignment with 1 reference gap and 1 read gap
	{ name   => "Gap penalties 6",
	  ref    => [ "ATTGTTCGTTTGTTCGTA" ],
	  reads  => [ "ATTGTTGTTTGATTCGTA" ], # budget = 3 + 18 * 3 = 57
	  args   =>   "--policy \"MMP=C30\\;SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;RDG=19,10\\;RFG=18,10\\;MIN=L,-3,-3\"",
	  report =>   "-a",
	  hits   => [ { 0 => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU,XC:6=1D5=1I6=" ],
	  cigar  => [ "6M1D5M1I6M" ] },

	# Alignment with 1 reference gap and 1 read gap, but not enough budget
	{ name   => "Gap penalties 7",
	  ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTGTTTGATTCGT" ], # budget = 3 + 16 * 3 = 51
	  args   =>   "--policy \"MMP=C30\\;SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;RDG=16,10\\;RFG=16,10\\;MIN=L,-3,-3\"",
	  report =>   "-a",
	  hits   => [ { "*" => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU" ],
	  cigar  => [ "*" ] },

	# Experiment with N filtering

	{ name => "N filtering 1",
	  ref      => [ "GAGACTTTATACGCATCGAACTATCGCTCTA" ],
	  reads    => [         "ATACGCATCGAAC" ],
	  #              0123456789012345678901234567890
	  #                        1         2         3
	  args     =>   "--policy \"NCEIL=L,0,0\"",
	  report   =>   "-a",
	  hits     => [ { 8 => 1 } ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:13=" ] },

	{ name => "N filtering 2",
	  ref      => [ "GAGACTTTATNCGCATCGAACTATCGCTCTA" ],
	  reads    => [         "ATACGCATCGAAC" ],
	  #              0123456789012345678901234567890
	  #                        1         2         3
	  args     =>   "--policy \"NCEIL=L,0,0\"",
	  report   =>   "-a",
	  hits     => [ { "*" => 1 } ] },

	{ name => "N filtering 3",
	  ref      => [ "GAGACTTTATACGCATCGAANTATCGCTCTA" ],
	  reads    => [         "ATACGCATCGAAC" ],
	  #              0123456789012345678901234567890
	  #                        1         2         3
	  args     =>   "--policy \"NCEIL=L,0,0\"",
	  report   =>   "-a",
	  hits     => [ { "*" => 1 } ] },

	{ name => "N filtering 4",
	  ref      => [ "GAGACTTTNTACGCATCGAACTATCGCTCTA" ],
	  reads    => [         "ATACGCATCGAAC" ],
	  #              0123456789012345678901234567890
	  #                        1         2         3
	  args     =>   "--policy \"NCEIL=L,0,0\"",
	  report   =>   "-a",
	  hits     => [ { "*" => 1 } ] },

	{ name => "N filtering 5",
	  ref      => [ "GAGACTTTATNCGCATCGAACTATCGCTCTA" ],
	  reads    => [         "ATACGCATCGAAC" ],
	  #              0123456789012345678901234567890
	  #                        1         2         3
	  args     =>   "--policy \"NCEIL=L,0,0.1\\;SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\"",
	  report   =>   "-a",
	  hits     => [ { 8 => 1 } ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:2=1X10=" ] },

	{ name => "N filtering 6",
	  ref      => [ "GAGACTTTNTACGCATCGAANTATCGCTCTA" ],
	  reads    => [         "ATACGCATCGAAC" ],
	  #              0123456789012345678901234567890
	  #                        1         2         3
	  args     =>   "--policy \"NCEIL=L,0,0.1\\;SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\"",
	  report   =>   "-a",
	  hits     => [ { "*" => 1 } ] },

	# No discordant alignment because one mate is repetitive.

	# Alignment with 1 reference gap
	{ ref    => [ "TTTTGTTCGTTTG" ],
	  reads  => [ "TTTTGTTCGATTTG" ], # budget = 3 + 14 * 3 = 45
	  args   =>   "--policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;MMP=C30\\;RDG=25,15\\;RFG=25,20\\;MIN=L,-3,-3\"",
	  report =>   "-a",
	  hits   => [ { 0 => 1 } ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:9=1I4=" ],
	  cigar  => [ "9M1I4M" ],
	  samoptflags => [
		{ "AS:i:-45" => 1, "NM:i:1"  => 1, "XO:i:1" => 1, "XG:i:1" => 1,
		  "YT:Z:UU" => 1, "MD:Z:13" => 1 },
	  ]
	},

	#  TTGTTCGTTTGTT
	# Tx
	# T x
	# G  x
	# T   x
	# T    x
	# C     x
	# G      x
	# A      x
	# T       x
	# T        x
	# T         x
	# G          x
	# T           x
	# T            x

	# Alignment with 1 reference gap
	{ ref    => [ "TTGTTCGTTTGTT" ],
	  reads  => [ "TTGTTCGATTTGTT" ], # budget = 3 + 14 * 3 = 45
	  args   =>   "--policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;MMP=C30\\;RDG=25,15\\;RFG=25,20\\;MIN=L,-3,-3\"",
	  report =>   "-a",
	  hits   => [ { 0 => 1 } ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:7=1I6=" ],
	  cigar  => [ "7M1I6M" ],
	  samoptflags => [
		{ "AS:i:-45" => 1, "NM:i:1"  => 1, "XO:i:1" => 1, "XG:i:1" => 1,
		  "YT:Z:UU" => 1, "MD:Z:13" => 1 },
	  ]
	},

	{ ref    => [ "ACNCA" ],
	  reads  => [ "CA" ],
	  args   => "",
	  report => "-a --policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,0,0\"",
	  hits   => [ { 3 => 1 } ],
	  edits  => [ ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:2=" ],
	  cigar  => [ "2M" ],
	  samoptflags => [
		{ "YT:Z:UU" => 1, "MD:Z:2" => 1 },
	  ]
	},

	{ name   => "N ceil = 0, 2 legit hits (1)",
	  ref    => [ "ACNCA" ],
	  reads  => [ "AC" ],
	  args   => "",
	  report => "-a --policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,0,0\"",
	  hits   => [ { 0 => 1 } ],
	  edits  => [ ],
	  flags => [ ] },

	{ name   => "N ceil = 0, 2 legit hits (2)",
	  ref    => [ "ACNCANNNNNNNNCGNNNNNNNNCG" ],
	#              0123456789012345678901234
	#              0         1         2
	  reads  => [ "CG" ],
	  args   => "",
	  report => "-a --policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,0,0\"",
	  hits   => [ { 13 => 2, 23 => 2 } ],
	  edits  => [ ],
	  cigar  => [ "2M", "2M" ],
	  samoptflags => [
		{ "YT:Z:UU" => 1, "MD:Z:2" => 1 },
		{ "YT:Z:UU" => 1, "MD:Z:2" => 1 },
	  ]
	},

	{ ref    => [ "ACNCANNNNNNAACGNNNNNNNACGAANNNNCGAAAN" ],
	#              0123456789012345678901234567890123456
	#              0         1         2         3
	  reads  => [ "CG" ],
	  args   => "",
	  report => "-a --policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,0,0\"",
	  hits   => [ { 13 => 2, 23 => 2, 31 => 2 } ],
	  edits  => [ ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:2=",
	             "XM:0,XP:0,XT:UU,XC:2=",
				 "XM:0,XP:0,XT:UU,XC:2=" ],
	  cigar  => [ "2M", "2M", "2M" ],
	  samoptflags => [
		{ "YT:Z:UU" => 1, "MD:Z:2" => 1 },
		{ "YT:Z:UU" => 1, "MD:Z:2" => 1 },
		{ "YT:Z:UU" => 1, "MD:Z:2" => 1 },
	  ]
	},

	{ ref    => [ "ACNCANNNNNNAACGNNNNNNNACGAANNNNCGAAAN" ],
	#              0123456789012345678901234567890123456
	#              0         1         2         3
	  reads  => [ "CG" ],
	  args   => "",
	  report => "-a --policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,0,0\"",
	  hits   => [ { 13 => 2, 23 => 2, 31 => 2 } ],
	  edits  => [ ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:2=",
	             "XM:0,XP:0,XT:UU,XC:2=",
	             "XM:0,XP:0,XT:UU,XC:2=" ],
	  cigar  => [ "2M", "2M", "2M" ],
	  samoptflags => [
		{ "YT:Z:UU" => 1, "MD:Z:2" => 1 },
		{ "YT:Z:UU" => 1, "MD:Z:2" => 1 },
		{ "YT:Z:UU" => 1, "MD:Z:2" => 1 },
	  ]
	},

	#
	# Alignment involving ambiguous reference character
	#

	# First read has non-compatible unambiguous charcacter (G for Y),
	# second read has compatible one
	{ ref    => [ "TTGTTYGT" ],
	  reads  => [ "TTGTTGGT", "TTGTTCGT" ],
	  args   => "",
	  report => "-a --policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,2,0\"",
	  hits   => [ { 0 => 1 }, { 0 => 1 } ],
	  norc   => 1,
	  edits  => [ "5:N>G", "5:N>C" ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:5=1X2=", "XM:0,XP:0,XT:UU,XC:5=1X2=" ],
	  cigar  => [ "8M", "8M" ],
	  samoptflags => [
		{ "AS:i:-1" => 1, "NM:i:1" => 1, "XM:i:1" => 1, "XN:i:1" => 1,
		  "YT:Z:UU" => 1, "MD:Z:5N2" => 1 },
		{ "AS:i:-1" => 1, "NM:i:1" => 1, "XM:i:1" => 1, "XN:i:1" => 1,
		  "YT:Z:UU" => 1, "MD:Z:5N2" => 1 },
	  ]
	},

	#
	# Alignment with multi-character read gap
	#

	# Relatively small example with a read gap extend
	{ ref    => [ "ATAACCTTCG" ],
	  reads  => [ "ATAATTCG" ], # 3 * 19 + 3 = 60
	  #                ^
	  #                4:CC>-
	  args   => "",
	  report => "-a --overhang --gbar 3 --policy \"MMP=C30\\;RDG=5,5\\;SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;RFG=25,20\\;MIN=L,-3,-3\"",
	  hits   => [ { 0 => 1 } ],
	  edits  => [ "4:CC>-" ],
	  flags  => [ "XM:0,XP:0,XT:UU,XC:4=2D4=" ],
	  cigar  => [ "4M2D4M" ],
	  samoptflags => [
		{ "AS:i:-15" => 1, "NM:i:2" => 1,
		  "XO:i:1" => 1, "XG:i:2" => 3, "YT:Z:UU" => 1, "MD:Z:4^CC4" => 1 }
	  ]
	},

	# Reads 1 and 2 don't have overhang, reads 3 and 4 overhang opposite ends
	{ ref    => [ "ATATGCCCCATGCCCCCCTCCG" ],
	  reads  => [ "ATATGCCCCCCCCCCTCCG" ], # 3 * 19 + 3 = 60
	  #                     ^
	  #                     9:ATG>-
	  args   =>   "--policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;MMP=C30\\;RDG=5,5\\;RFG=25,15\\;MIN=L,-3,-3\"",
	  hits   => [ { 0 => 1 } ],
	  edits  => [ "9:ATG>-" ],
	  norc   => 1,
	  flags => [ "XM:0,XP:0,XT:UU,XC:9=3D10=" ],
	  cigar  => [ "9M3D10M" ],
	  samoptflags => [
		{ "AS:i:-20" => 1, "NM:i:3" => 1,
		  "XO:i:1" => 1, "XG:i:3" => 3, "YT:Z:UU" => 1, "MD:Z:9^ATG10" => 1 }
	  ]
	},

	# Reads 1 and 2 don't have overhang, reads 3 and 4 overhang opposite ends
	{ ref    => [ "ATATGCCCCATGCCCCCCTCCG" ],
	  reads  => [ "CGGAGGGGGGGGGGCATAT" ],
	  #            ATATGCCCCCCCCCCTCCG
	  #                     ^
	  #                     10:GTA>-
	  args   => "",
	  report => "-a --overhang --policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;MMP=C30\\;RDG=5,5\\;RFG=25,20\\;MIN=L,-3,-3\"",
	  hits   => [ { 0 => 1 } ],
	  edits  => [ "10:GTA>-" ],
	  norc   => 1,
	  flags => [ "XM:0,XP:0,XT:UU,XC:9=3D10=" ],
	  cigar  => [ "9M3D10M" ],
	  samoptflags => [
		{ "AS:i:-20" => 1, "NM:i:3" => 1,
		  "XO:i:1" => 1, "XG:i:3" => 3, "YT:Z:UU" => 1, "MD:Z:9^ATG10" => 1 }
	  ]
	},

	# 1 discordant alignment and one concordant alignment.  Discordant because
	# the fragment is too long.

	{ name => "Simple paired-end 13",
	  ref    => [ "TTTATAAAAATATTTCCCCCCCCCCCCCCTGTCGCTACCGCCCCCCCCCCC" ],
	#              012345678901234567890123456789012345678901234567890
	#              0         1         2         3         4         5
	#                 ATAAAAATAT                 GTCGCTACCG
	#                 ATAAAAATAT                TGTCGCTACC
	#                 ATAAAAATAT               CTGTCGCTAC
	#                 ATAAAAATAT              CCTGTCGCTA
	#                  TAAAAATATT                GTCGCTACCG
	#                  TAAAAATATT               TGTCGCTACC
	#                  TAAAAATATT              CTGTCGCTAC
	#                  TAAAAATATT             CCTGTCGCTA
	#              012345678901234567890123456789012345678901234567890
	#              0         1         2         3         4         5
	#                 -----------------------------------
	#                 012345678901234567890123456789012345678901234567
	#                 0         1         2         3         4
	  mate1s   => [ "ATAAAAATAT", "ATAAAAATAT", "ATAAAAATAT", "ATAAAAATAT",
	                "TAAAAATATT", "TAAAAATATT", "TAAAAATATT", "TAAAAATATT", ],
	  mate2s   => [ "GTCGCTACCG", "TGTCGCTACC", "CTGTCGCTAC", "CCTGTCGCTA",
	                "GTCGCTACCG", "TGTCGCTACC", "CTGTCGCTAC", "CCTGTCGCTA" ],
	  mate1fw => 1,  mate2fw => 1,
	  args     =>   "-I 0 -X 35",
	  # Not really any way to flag an alignment as discordant
	  pairhits => [ { "3,30" => 1 }, { "3,29" => 1 }, { "3,28" => 1 }, { "3,27" => 1 },
	                { "4,30" => 1 }, { "4,29" => 1 }, { "4,28" => 1 }, { "4,27" => 1 } ],
	  flags    => [ "XM:0,XP:0,XT:DP,XC:10=", "XM:0,XP:0,XT:DP,XC:10=",
	                "XM:0,XP:0,XT:CP,XC:10=", "XM:0,XP:0,XT:CP,XC:10=",
	                "XM:0,XP:0,XT:DP,XC:10=", "XM:0,XP:0,XT:CP,XC:10=",
					"XM:0,XP:0,XT:CP,XC:10=", "XM:0,XP:0,XT:CP,XC:10=" ] },

	# 1 discordant alignment and one concordant alignment.  Discordant because
	# the fragment is too long.

	{ name => "Simple paired-end 12",
	  ref    => [ "TTTATAAAAATATTTCCCCCCCCCCCCCCGGGCCCGCCCGCCCCCCCCCCC" ],
	#                 ATAAAAATAT                 GGCCCGCCCG
	#                 ATAAAAATAT              CCGGGCCCGC
	#              012345678901234567890123456789012345678901234567890
	#              0         1         2         3         4         5
	#                 -------------------------------------
	#                 012345678901234567890123456789012345678901234567
	  mate1s => [ "ATAAAAATAT", "ATAAAAATAT" ],
	  mate2s => [ "GGCCCGCCCG", "CCGGGCCCGC" ],
	  mate1fw => 1,  mate2fw => 1,
	  args   =>   "-I 0 -X 36",
	  # Not really any way to flag an alignment as discordant
	  pairhits => [ { "3,30" => 1 }, { "3,27" => 1 } ],
	  flags => [ "XM:0,XP:0,XT:DP,XC:10=", "XM:0,XP:0,XT:CP,XC:10=" ] },

	# 1 discordant alignment.  Discordant because the fragment is too long.

	{ name => "Simple paired-end 11",
	  ref    => [ "TTTATAAAAATATTTCCCCCCCCCCCCCCCCGATCGCCCGCCCCCCCCCCC" ],
	#                 ATAAAAATAT                 CGATCGCCCG
	#              012345678901234567890123456789012345678901234567890
	#              0         1         2         3         4         5
	#                 -------------------------------------
	#                 012345678901234567890123456789012345678901234567
	  mate1s => [ "ATAAAAATAT" ],
	  mate2s => [ "CGATCGCCCG" ],
	  mate1fw => 1,  mate2fw => 1,
	  args   =>   "-I 0 -X 36",
	  # Not really any way to flag an alignment as discordant
	  pairhits => [ { "3,30" => 1 } ],
	  flags => [ "XM:0,XP:0,XT:DP,XC:10=" ] },

	# 1 discordant alignment.  Discordant because the fragment is too short.

	{ name => "Simple paired-end 10",
	  ref    => [ "TTTATAAAAATATTTCCCCCCGATCGCCCGCCCCCCCCCCC" ],
	#                 ATAAAAATAT       CGATCGCCCG
	#              01234567890123456789012345678901234567890
	#              0         1         2         3         4
	#                 ---------------------------
	#                 012345678901234567890123456
	  mate1s => [ "ATAAAAATAT" ],
	  mate2s => [ "CGATCGCCCG" ],
	  mate1fw => 1,  mate2fw => 1,
	  args   =>   "-I 28 -X 80",
	  # Not really any way to flag an alignment as discordant
	  pairhits => [ { "3,20" => 1 } ],
	  flags => [ "XM:0,XP:0,XT:DP,XC:10=" ] },

	# Like 6, but with -M limit

	{ name => "Simple paired-end 9",
	  ref    => [ "CCCATATATATATCCTCCCATATATATATCCCTCCCCATATATATATCCCTTTTCCTTTCGCGCGCGCGTTTCCCCCCCCC" ],
	#                 ATATATATAT      ATATATATAT        ATATATATAT            CGCGCGCGCG
	#              012345678901234567890123456789012345678901234567890123456789012345678901234567890
	#              0         1         2         3         4         5         6         7         8
	  mate1s  => [ "ATATATATAT" ],
	  mate2s  => [ "CGCGCGCGCG" ],
	  mate1fw => 1,  mate2fw => 0,
	  args    => "-I 0 -X 80",
	  report  => "-M 2",
	  lines   => 2,
	  pairhits => [ { "3,59" => 1, "19,59" => 1, "37,59" => 1 } ],
	  hits_are_superset => [ 1 ],
	  flags  => [ "XM:1,XP:1,XT:CP,XC:10=", "XM:1,XP:1,XT:CP,XC:10=" ] },

	# Like 6, but without -m limit

	{ name => "Simple paired-end 8",
	  ref    => [ "CCCATATATATATCCTCCCATATATATATCCCTTCCCATATATATATCCCTTTTTTTTTCGCGCGCGCGTTTCCCCCCCCC" ],
	#                 ATATATATAT      ATATATATAT        ATATATATAT            CGCGCGCGCG
	#              012345678901234567890123456789012345678901234567890123456789012345678901234567890
	#              0         1         2         3         4         5         6         7         8
	  mate1s => [ "ATATATATAT" ],
	  mate2s => [ "CGCGCGCGCG" ],
	  mate1fw => 1,  mate2fw => 0,
	  args   =>   "-I 0 -X 80",
	  pairhits => [ { "3,59" => 1, "19,59" => 1, "37,59" => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:CP,XC:10=" ] },

	# Paired-end read, but only one mate aligns

	{ name => "Simple paired-end 2; no --no-mixed",
	  ref    => [ "CCCATATATATATCCCTTTTTTTCCCCCCCCCCTTCGCGCGCGCGTTTCCCCC" ],
	#                 ATATATATAT                      CGCGCGCGCG
	#              01234567890123456789012345678901234567890123456789012
	#              0         1         2         3         4         5
	  mate1s => [ "ATATATATAT" ],
	  mate2s => [ "CCCCCGGGGG" ],
	  mate1fw => 1,  mate2fw => 1,
	  args   =>   "-I 0 -X 50 --nofw",
	  nofw   => 1,
	  pairhits  => [ { "*,3"  => 1 } ],
	  flags_map => [ {  3     => "XM:0,XP:0,XT:UP,XC:10=",
	                   "*"    => "XM:0,XP:0,XT:UP" } ],
	  cigar_map => [{
		3 => "10M",
		"*" => "*"
	  }],
	  samoptflags_map => [{
		3 => {
			"MD:Z:10"  => 1, # mismatching positions/bases
			"YT:Z:UP"  => 1, # type of alignment (concordant/discordant/etc)
		},
		"*" => {
			"YT:Z:UP"  => 1, # type of alignment (concordant/discordant/etc)
		}
	  }]
	},

	{ name => "Simple paired-end 2; --no-mixed",
	  ref    => [ "CCCATATATATATCCCTTTTTTTCCCCCCCCTTTTCGCGCGCGCGTTTCCCCC" ],
	#                 ATATATATAT                      CGCGCGCGCG
	#              01234567890123456789012345678901234567890123456789012
	#              0         1         2         3         4         5
	  mate1s => [ "ATATATATAT" ],
	  mate2s => [ "CCCCCGGGGG" ],
	  mate1fw => 1,  mate2fw => 1,
	  args   =>   "-I 0 -X 50 --no-mixed",
	  pairhits => [ { "*,*" => 1 } ] },

	# Simple paired-end alignment

	{ name => "Simple paired-end 1",
	  ref    => [ "CCCATATATATATCCCTTTTTTTCCCCCCCCTTTTCGCGCGCGCGTTTTCCCC" ],
	#                 ATATATATAT                      CGCGCGCGCG
	#              01234567890123456789012345678901234567890123456789012
	#              0         1         2         3         4         5
	  mate1s => [ "ATATATATAT" ],
	  mate2s => [ "CGCGCGCGCG" ],
	  mate1fw => 1,  mate2fw => 1,
	  args   =>   "-I 0 -X 50",
	  pairhits => [ { "3,35" => 1 } ],
	  flags => [ "XM:0,XP:0,XT:CP,XC:10=" ],
	  cigar_map => [{
		3  => "10M",
		35 => "10M"
	  }],
	  samoptflags_map => [{
		3 => {
			"MD:Z:10"  => 1, # mismatching positions/bases
			"YT:Z:CP"  => 1, # type of alignment (concordant/discordant/etc)
		},
		35 => {
			"MD:Z:10"  => 1, # mismatching positions/bases
			"YT:Z:CP"  => 1, # type of alignment (concordant/discordant/etc)
		}
	  }]
	},

	# Check that pseudo-random generation is always the same for
	# same-sequence, same-name reads

	{ ref    => [ "AAAAAAAAAAAAAAAAAAAAAAAAACCCCCCCCCCCCCCCCCCCCCCCC" ],
	  reads  => [ "AA", "AA", "AA", "AA", "CC", "CC", "CC", "CC", "AA", "AA", "AA", "AA", "CC", "CC", "CC", "CC" ],
	  names  => [ "r1", "r1", "r1", "r1", "r2", "r2", "r2", "r2", "r3", "r3", "r3", "r3", "r4", "r4", "r4", "r4" ],
	  args   => "",
	  check_random => 1,
	  report => "-k 1" },

	{ ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTGTTCGT" ],
	  report => "-M 1",
	  hits   => [ { 0 => 1, 8 => 1 } ],
	  flags  => [ "XM:1,XP:0,XT:UU,XC:8=" ],
	  hits_are_superset => [ 1 ],
	  cigar  => [ "8M" ],
	  samoptflags => [
		{ "YM:i:1" => 1, "YT:Z:UU" => 1, "MD:Z:8" => 1, "YM:i:1" => 1 }
	  ],
	},

	# Read 3 overhangs right end
	{ ref    => [ "TTGTTCGT"  ],
	  reads  => [   "GTTCGTA" ],
	  args   => "--overhang --policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;NCEIL=L,2,0\"",
	  hits   => [ { 2 => 1 } ],
	  flags => [ "XM:0,XP:0,XT:UU,XC:6=1X" ] },

	# Mess with arguments

	# Default should be 1-mismatch, so this shouldn't align
	{ ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTATTAGT" ],
	  args   => "",
	  hits   => [ { "*" => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU" ],
	  cigar  => [ "*" ],
	  samoptflags => [{ "YT:Z:UU" => 1 }],
	},

	# Shouldn't align with 0 mismatches either
	{ ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTATTAGT" ],
	  args   => "--policy SEED=0",
	  hits   => [ { "*" => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU" ],
	  cigar  => [ "*" ],
	  samoptflags => [{ "YT:Z:UU" => 1 }],
	},

	# Should align with 0 mismatches if we can wedge a seed into the 2
	# matching characters between the two mismatches.  Here we fail to
	# wedge a length-3 seed in (there's no room)
	{ ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [ "TTATTAGT" ],
	  args   => "--policy \"SEED=0\\;SEEDLEN=3\\;IVAL=C,1,0\\;MMP=C1\"",
	  hits   => [ { "*" => 1 } ],
	  flags  => [ "XM:0,XP:0,XT:UU" ],
	  cigar  => [ "*" ],
	  samoptflags => [{ "YT:Z:UU" => 1 }],
	},

	# Should align with 0 mismatches if we can wedge a seed into the 2
	# matching characters between the two mismatches.  Here we wedge a
	# length-2 seed in
	{ ref    => [ "TTGTTCGTTTGTTCGT" ],
	  reads  => [      "TTATTAGT" ],
	  args   => "--policy \"SEED=0\\;SEEDLEN=2\\;IVAL=C,1,0\\;MMP=C1\"",
	  #
	  # TTGTTCGTTTGTTCGT TTGTTCGTTTGTTCGT TTGTTCGTTTGTTCGT
	  # || || ||            ||  |             |  || ||
	  # TTATTAGT            TTATTAGT          TTATTAGT
	  #
	  # TTGTTCGTTTGTTCGT TTGTTCGTTTGTTCGT TTGTTCGTTTGTTCGT
	  #         ||  |           ||  |             || || ||
	  #      TTATTAGT           TTATTAGT          TTATTAGT
	  #
	  hits   =>   [ { 0 => 1, 3 => 1, 4 => 1,
	                  5 => 1, 7 => 1, 8 => 1} ],
	  flag_map => [ { 0 => "XM:0,XP:0,XT:UU,XC:2=1X2=1X2=",
	                  3 => "XM:0,XP:0,XT:UU,XC:2=2X1=3X",
					  4 => "XM:0,XP:0,XT:UU,XC:1=2X2=1X2=",
					  5 => "XM:0,XP:0,XT:UU,XC:3X2=2X1=",
					  7 => "XM:0,XP:0,XT:UU,XC:2=2X1=3X",
					  8 => "XM:0,XP:0,XT:UU,XC:2=1X2=1X2="} ],
	  cigar_map => [ { 0 => "8M", 3 => "8M", 4 => "8M",
	                   5 => "8M", 7 => "8M", 8 => "8M" } ],
	  samoptflags_map => [{
		0 => { "AS:i:-2" => 1, "XS:i:-2" => 1, "NM:i:2" => 1, "XM:i:2" => 1,
		       "YT:Z:UU" => 1, "MD:Z:2G2C2"    => 1 },
		3 => { "AS:i:-5" => 1, "XS:i:-2" => 1, "NM:i:5" => 1, "XM:i:5" => 1,
		       "YT:Z:UU" => 1, "MD:Z:2C0G1T0T0G0" => 1 },
		4 => { "AS:i:-3" => 1, "XS:i:-2" => 1, "NM:i:3" => 1, "XM:i:3" => 1,
		       "YT:Z:UU" => 1, "MD:Z:1C0G2T2"   => 1 },
		5 => { "AS:i:-5" => 1, "XS:i:-2" => 1, "NM:i:5" => 1, "XM:i:5" => 1,
		       "YT:Z:UU" => 1, "MD:Z:0C0G0T2G0T1" => 1 },
		7 => { "AS:i:-5" => 1, "XS:i:-2" => 1, "NM:i:5" => 1, "XM:i:5" => 1,
		       "YT:Z:UU" => 1, "MD:Z:2T0G1T0C0G0" => 1 },
		8 => { "AS:i:-2" => 1, "XS:i:-2" => 1, "NM:i:2" => 1, "XM:i:2" => 1,
		       "YT:Z:UU" => 1, "MD:Z:2G2C2"    => 1 },
	  }],
	},
);

##
# Take a list of reference sequences and write them to a temporary
# FASTA file of the given name.
#
sub writeFasta($$) {
	my ($l, $fa) = @_;
	open(FA, ">$fa") || die "Could not open $fa for writing";
	my $idx = 0;
	for(my $i = 0; $i < scalar(@$l); $i++) {
		print FA ">$idx\n".$l->[$i]."\n";
		$idx++;
	}
	close(FA);
}

sub convertFastqToSRA($$$) {
	my ($fq1, $fq2, $pe) = @_;
	my $latf_cmd;

	if ($pe) {
		if (ref $fq1 eq "ARRAY") {
			$latf_cmd = "latf-load -p ILLUMINA -o .test_sra --quality PHRED_33 " . join(" ", @$fq1) . " " . join(" ", @$fq2);
		} else {
			$latf_cmd = "latf-load -p ILLUMINA -o .test_sra --quality PHRED_33 " . $fq1 . " " . $fq2;
		}
	} else {
		if (ref $fq1 eq "ARRAY") {
			$latf_cmd = "latf-load -p ILLUMINA -o .test_sra --quality PHRED_33 " . join(" ", @$fq1);
		} else {
			$latf_cmd = "latf-load -p ILLUMINA -o .test_sra --quality PHRED_33 " . $fq1;
		}
	}

	print "$latf_cmd\n";
	system($latf_cmd);
}

sub convertFastqToBAM($$$) {
        my ($fq1, $fq2, $pe) = @_;
        my $bam_cmd;

        if (! -e ".dummy_index.1.bt2") {
                system("./bowtie2-build -c NNNGNNN .dummy_index");
        }

        if ($pe) {
                if (ref $fq1 eq "ARRAY") {
                        $bam_cmd = "./bowtie2 -x .dummy_index -1" . join(",", @$fq1) . " -2 " . join(",", @$fq2) . "| samtools view -b -o .test.bam";
                } else {
                        $bam_cmd = "./bowtie2 -x .dummy_index -1 $fq1 -2 $fq2 | samtools view -b -o .test.bam";
                }
        } else {
                if (ref $fq1 eq "ARRAY") {
                        $bam_cmd = "./bowtie2 -x .dummy_index " . join(",", @$fq1) . " | samtools view -b -o .test.bam";
                } else {
                        $bam_cmd = "./bowtie2 -x .dummy_index $fq1 | samtools view -b -o .test.bam";
                }
        }

        print "$bam_cmd\n";
        system($bam_cmd);
}

##
# Take a lists of named reads/mates and write them to appropriate
# files.
#
sub writeReads($$$$$$$$$$$) {
	my (
		$reads,
		$quals,
		$mate1s,
		$qual1s,
		$mate2s,
		$qual2s,
		$names,
		$test_sra,
	        $has_poison_reads,
		$fq1,
		$fq2) = @_;

	my $pe = (defined($mate1s) && $mate1s ne "");
	if($pe) {
		for (0..scalar(@$mate1s)-1) {
			open(FQ1, defined($compressed) ? "| gzip -c >$fq1->[$_]" : ">$fq1->[$_]") || die "Could not open '$fq1->[$_]' for writing";
			open(FQ2, defined($compressed) ? "| gzip -c >$fq2->[$_]" : ">$fq2->[$_]") || die "Could not open '$fq2->[$_]' for writing";

			my $m1 = $mate1s->[$_];
			my $m2 = $mate2s->[$_];
			my $q1 = $qual1s->[$_];
			my $q2 = $qual2s->[$_];
			my $nm = $names->[$_];

			defined($m1) || die;
			defined($m2) || die;

			if ($m1 eq "" || $m2 eq "") {
				$$has_poison_reads = 1;
			}

			$q1 = $q1 || ("I" x length($m1));
			$q2 = $q2 || ("I" x length($m2));
			$nm = $nm || "r$_";
			if ($test_sra) {
				print FQ1 "\@$nm#$_/1\n$m1\n+\n$q1\n";
				print FQ2 "\@$nm#$_/2\n$m2\n+\n$q2\n";
			} else {
				print FQ1 "\@$nm/1\n$m1\n+\n$q1\n";
				print FQ2 "\@$nm/2\n$m2\n+\n$q2\n";
			}

			close(FQ1);
			close(FQ2);
		}
	} else {
		for (0..scalar(@$reads)-1) {
			open(FQ1, defined($compressed) ? "| gzip -c >$fq1->[$_]" : ">$fq1->[$_]") || die "Could not open '$fq1->[$_]' for writing";

			my $read = $reads->[$_];
			defined($read) || die;

			if ($read eq "") {
				$$has_poison_reads = 1;
			}

			my $qual = $quals->[$_];
			my $nm = $names->[$_];

			$qual = $qual || ("I" x length($read));
			$nm = $nm || "r$_";
			print FQ1 "\@$nm\n$read\n+\n$qual\n";
			close(FQ1);
		}
	}
}

##
# Run bowtie2 with given arguments
#
sub runbowtie2($$$$$$$$$$$$$$$$$$$$$$$$$) {

	my (
		$do_build,
		$large_idx,
		$binary_type,
		$args,
		$fa,
		$reportargs,       #5
		$read_file_format,
		$read_file,
		$mate1_file,
		$mate2_file,
		$reads,
		$quals,
		$mate1s,
		$qual1s,
		$mate2s,
		$qual2s,
		$names,
                $test_bam,
		$test_sra,
		$has_poison_reads,
		$ls,
		$rawls,
		$header_ls,
		$raw_header_ls,
		$should_abort) = @_;

	my  $idx_type = "";
	$args .= " --quiet";
	$reportargs = "-a" unless defined($reportargs);
	$args .= " $reportargs";
	if ($large_idx){
	    $idx_type = "--large-index";
	}
	# Write the reference to a fasta file
	print "References:\n";
	open(FA, $fa) || die;
	while(<FA>) { print $_; }
	close(FA);
	if($do_build) {
		my $build_args = "";
		my $cmd = "$bowtie2_build $idx_type --quiet --sanity $build_args $fa .simple_tests.tmp";
		print "$cmd\n";
		system($cmd);
		($? == 0) || die "Bad exitlevel from bowtie2-build: $?";
	}
	my $pe = (defined($mate1s) && $mate1s ne "");
	$pe = $pe || (defined($mate1_file));
	my $cmd;
	my $mate1arg;
	my $mate2arg;
	my $readarg;
	my $formatarg = "-c";
	my $batch_size = int(rand(16) + 1);
	my ($readstr, $m1str, $m2str) = (undef, undef, undef);
	$readstr = join(",", @$reads)  if defined($reads);
	$m1str   = join(",", @$mate1s) if defined($mate1s);
	$m2str   = join(",", @$mate2s) if defined($mate2s);

	if (defined($read_file) || defined($mate1_file)) {
		defined($read_file_format) || die;
		my $ext = "";
		if($read_file_format eq "fastq") {
			$formatarg = "-q";
			$ext = ".fq";
		} elsif($read_file_format eq "tabbed") {
			$formatarg = "--tab5";
			$ext = ".tab";
		} elsif($read_file_format eq "cline_reads") {
			$formatarg = "-c";
			$readarg = $read_file;
			$mate1arg = $mate1_file;
			$mate2arg = $mate2_file;
		} elsif($read_file_format eq "cont_fasta_reads") {
			$formatarg = "";
			$readarg = $read_file;
			$mate1arg = $mate1_file;
			$mate2arg = $mate2_file;
		} elsif($read_file_format eq "fasta") {
			$formatarg = "-f";
			$ext = ".fa";
		} elsif($read_file_format eq "qseq") {
			$formatarg = "--qseq";
			$ext = "_qseq.txt";
		} elsif($read_file_format eq "raw") {
			$formatarg = "-r";
			$ext = ".raw";
		} else {
			die "Bad format: $read_file_format";
		}
		if($formatarg ne "-c") {
			my $cmd = ">";
			if (defined($compressed)) {
				$cmd = "| gzip -c" . $cmd;
				$ext = $ext . ".gz";
			}
			if(defined($read_file)) {
				# Unpaired
				open(RD, $cmd . ".simple_tests$ext") || die;
				print RD $read_file;
				close(RD);
				$readarg = ".simple_tests$ext";
			} else {
				defined($mate1_file) || die;
				defined($mate2_file) || die;

				if ($test_sra) {
					$mate1_file =~ s/r(\d+)/r#\1\/1/g;
					$mate2_file =~ s/r(\d+)/r#\1\/2/g;
				}
				# Paired
				open(M1, $cmd . ".simple_tests.1$ext") || die;
				print M1 $mate1_file;
				close(M1);
				open(M2, $cmd . ".simple_tests.2$ext") || die;
				print M2 $mate2_file;
				close(M2);
				$mate1arg = ".simple_tests.1$ext";
				$mate2arg = ".simple_tests.2$ext";
			}
		}
	} else {
		$mate1arg = [];
		$mate2arg = [];
		my $ext = $compressed ? ".fq.gz" : ".fq";
		my $base_filename = ".simple_tests";

		for (0 .. scalar($pe ? @$mate1s : @$reads) - 1) {
			my $f = $base_filename . ".1" . ('a' .. 'z')[$_] . $ext;
			push @$mate1arg, $f;

			if ($pe) {
				$f = $base_filename . ".2" . ('a' .. 'z')[$_] . $ext;
				push @$mate2arg, $f;
			}
		}

		writeReads(
			$reads,
			$quals,
			$mate1s,
			$qual1s,
			$mate2s,
			$qual2s,
			$names,
			$test_sra,
			$has_poison_reads,
			$mate1arg,
			$mate2arg);

		$formatarg = "-q";
		$readarg = $mate1arg;
	}

	if ($binary_type eq "release") {
		$binary_type = "";
	}
	else {
		$binary_type = "--" . $binary_type;
	}

	if ($test_sra) {
		convertFastqToSRA(defined($readarg) ? $readarg : $mate1arg, $mate2arg, $pe);
		$cmd = "$bowtie2 $binary_type @ARGV $idx_type $args --reads-per-batch $batch_size -x .simple_tests.tmp --sra-acc .test_sra";
	} elsif ($test_bam) {
                convertFastqToBAM(defined($readarg) ? $readarg : $mate1arg, $mate2arg, $pe);
                $cmd = "$bowtie2 $binary_type @ARGV $idx_type $args --reads-per-batch $batch_size -x .simple_tests.tmp -b .test.bam";
                if ($pe) {
                        $cmd = $cmd . " --align-paired-reads";
                }
        } else {
		if($pe) {
			# Paired-end case
			if (ref $mate1arg eq "ARRAY") {
				$cmd = "$bowtie2 $binary_type @ARGV $idx_type $args --reads-per-batch $batch_size -x .simple_tests.tmp $formatarg -1 " . join(",", @$mate1arg) . " -2 " . join(",", @$mate2arg);
			}
			else {
				$cmd = "$bowtie2 $binary_type @ARGV $idx_type $args --reads-per-batch $batch_size -x .simple_tests.tmp $formatarg -1 $mate1arg -2 $mate2arg";
			}
		} else {
			# Unpaired case
			if (ref $readarg eq "ARRAY") {
				$cmd = "$bowtie2 $binary_type @ARGV $idx_type $args --reads-per-batch $batch_size -x .simple_tests.tmp $formatarg " . join(",", @$readarg);
			}
			else {
				$cmd = "$bowtie2 $binary_type @ARGV $idx_type $args --reads-per-batch $batch_size -x .simple_tests.tmp $formatarg $readarg";
			}
		}
        }

	print "$cmd\n";
	open(BT, "$cmd |") || die "Could not open pipe '$cmd |'";
	while(<BT>) {
		print $_;
		chomp;
		if(substr($_, 0, 1) eq "@") {
			push @$header_ls, [ split(/\t/, $_, -1) ];
			push @$raw_header_ls, $_;
		} else {
			push @$ls, [ split(/\t/, $_, -1) ];
			push @$rawls, $_;
		}
	}
	close(BT);
	($? == 0 ||  $should_abort) || die "bowtie2 aborted with exitlevel $?\n";
	($? != 0 || !$should_abort) || die "bowtie2 failed to abort!\n";
}

##
# Compare a hash ref of expected SAM flags with a hash ref of observed SAM
# flags.
#
sub matchSamOptionalFlags($$) {
	my ($flags, $ex_flags) = @_;
	my %ex = ();
	for(keys %$ex_flags) {
		my ($nm, $ty, $vl) = split(/:/, $_);
		defined($vl) || die "Could not parse optional flag field \"$_\"";
		($ex{$nm}{ty}, $ex{$nm}{vl}) = ($ty, $vl);
	}
	for(keys %$flags) {
		my ($ex_ty, $ex_vl);
		if(defined($ex{$_})) {
			($ex_ty, $ex_vl) = ($ex{$_}{ty}, $ex{$_}{vl});
		} else {
			($ex_ty, $ex_vl) = ("i", "0");
		}
		defined($ex_ty) || die;
		defined($ex_vl) || die;
		my ($ty, $vl) = ($flags->{$_}{ty}, $flags->{$_}{vl});
		defined($ty) || die;
		defined($vl) || die;
		$ex_ty eq $ty ||
			die "Expected SAM optional flag $_ to have type $ex_ty, had $ty";
		$ex_vl eq $vl ||
			die "Expected SAM optional flag $_ to have value $ex_vl, had $vl";
	}
	return 1;
}

my $tmpfafn = ".simple_tests.pl.fa";
my $last_ref = undef;
foreach my $large_idx (undef,1) {
	foreach my $binary_type ("release", "debug", "sanitized") {
		for (my $ci = 0; $ci < scalar(@cases); $ci++) {
			my $c = $cases[$ci];
			last unless defined($c);
			# If there's any skipping of cases to be done, do it here prior to the
			# eq_deeply check
			my $do_build = 0;
			unless(defined($last_ref) && eq_deeply($c->{ref}, $last_ref)) {
				writeFasta($c->{ref}, $tmpfafn);
				$do_build = 1;
			}
			$last_ref = $c->{ref};
			# For each set of arguments...
			my $case_args = $c->{args};
			$case_args = "" unless defined($case_args);
			my $first = 1; # did we build the index yet?
			# Forward, then reverse-complemented
			my $fwlo = ($c->{nofw} ? 1 : 0);
			my $fwhi = ($c->{norc} ? 0 : 1);
			for(my $fwi = $fwlo; $fwi <= $fwhi; $fwi++) {
				my $fw = ($fwi == 0);
				my $sam = 1;

				my $reads      = $c->{reads};
				my $quals      = $c->{quals};
				my $m1s        = $c->{mate1s};
				my $q1s        = $c->{qual1s};
				my $m2s        = $c->{mate2s};
				my $q2s        = $c->{qual2s};

				my $read_file  = undef;
				my $mate1_file = undef;
				my $mate2_file = undef;

				$read_file  = $c->{fastq}   if defined($c->{fastq});
				$read_file  = $c->{tabbed}  if defined($c->{tabbed});
				$read_file  = $c->{fasta}   if defined($c->{fasta});
				$read_file  = $c->{qseq}    if defined($c->{qseq});
				$read_file  = $c->{raw}     if defined($c->{raw});
				$read_file  = $c->{cline_reads} if defined($c->{cline_reads});
				$read_file  = $c->{cont_fasta_reads} if defined($c->{cont_fasta_reads});

				$mate1_file = $c->{fastq1}  if defined($c->{fastq1});
				$mate1_file = $c->{tabbed1} if defined($c->{tabbed1});
				$mate1_file = $c->{fasta1}  if defined($c->{fasta1});
				$mate1_file = $c->{qseq1}   if defined($c->{qseq1});
				$mate1_file = $c->{raw1}    if defined($c->{raw1});
				$mate1_file = $c->{cline_reads1} if defined($c->{cline_reads1});
				$mate1_file = $c->{cont_fasta_reads1} if defined($c->{cont_fasta_reads1});

				$mate2_file = $c->{fastq2}  if defined($c->{fastq2});
				$mate2_file = $c->{tabbed2} if defined($c->{tabbed2});
				$mate2_file = $c->{fasta2}  if defined($c->{fasta2});
				$mate2_file = $c->{qseq2}   if defined($c->{qseq2});
				$mate2_file = $c->{raw2}    if defined($c->{raw2});
				$mate2_file = $c->{cline_reads2} if defined($c->{cline_reads2});
				$mate2_file = $c->{cont_fasta_reads2} if defined($c->{cont_fasta_reads2});

				my $read_file_format = undef;
				if(!defined($reads) && !defined($m1s) && !defined($m2s)) {
					defined($read_file) || defined($mate1_file) || die;
					$read_file_format = "fastq"  if defined($c->{fastq})  || defined($c->{fastq1});
					$read_file_format = "tabbed" if defined($c->{tabbed}) || defined($c->{tabbed});
					$read_file_format = "fasta"  if defined($c->{fasta})  || defined($c->{fasta1});
					$read_file_format = "qseq"   if defined($c->{qseq})   || defined($c->{qseq1});
					$read_file_format = "raw"    if defined($c->{raw})    || defined($c->{raw1});
					$read_file_format = "cline_reads" if defined($c->{cline_reads}) || defined($c->{cline_reads1});
					$read_file_format = "cont_fasta_reads" if defined($c->{cont_fasta_reads}) || defined($c->{cont_fasta_reads1});
					next unless $fw;
				}
				# Run bowtie2
                                my $test_bam = 0;
                                my $test_sra = 0;
				my $reads_are_fastq = 0;
				# flag to indicate whether sample fastq has empty sequences
				# qualities. latf-load cannot convert such reads to SRA, so
				# it is best if we ignore them.
				my $has_poison_reads = 0;

				print $c->{name}." " if defined($c->{name});
				print "(fw:".($fw ? 1 : 0).", sam:$sam)\n";
				my $mate1fw = 1;
				my $mate2fw = 0;
				$mate1fw = $c->{mate1fw} if defined($c->{mate1fw});
				$mate2fw = $c->{mate2fw} if defined($c->{mate2fw});
				if(!$fw) {
					# Reverse-complement the reads
					my @s = (); @s = @$reads if defined($reads);
					my @q = (); @q = @$quals if defined($quals);
					# Reverse-complement mates and switch mate1 with mate2
					my @m1 = (); @m1 = @$m1s if defined($m1s);
					my @m2 = (); @m2 = @$m2s if defined($m2s);
					my @q1 = (); @q1 = @$q1s if defined($q1s);
					my @q2 = (); @q2 = @$q2s if defined($q2s);
					for(0..scalar(@s)-1) {
						$s[$_] = DNA::revcomp($s[$_]);
						$q[$_] = reverse $q[$_] if $_ < scalar(@q);
					}
					if($mate1fw == $mate2fw) {
						for(0..$#m1) { $m1[$_] = DNA::revcomp($m1[$_]); }
						for(0..$#m2) { $m2[$_] = DNA::revcomp($m2[$_]); }
						for(0..$#q1) { $q1[$_] = reverse $q1[$_]; }
						for(0..$#q2) { $q2[$_] = reverse $q2[$_]; }
					}
					$reads = \@s if defined($reads);
					$quals = \@q if defined($quals);
					$m1s   = \@m2 if defined($m1s);
					$q1s   = \@q2 if defined($q1s);
					$m2s   = \@m1 if defined($m2s);
					$q2s   = \@q1 if defined($q2s);
				}
				my $a = $case_args;
				if(defined($m2s)) {
					$a .= " --";
					$a .= ($mate1fw ? "f" : "r");
					$a .= ($mate2fw ? "f" : "r");
				}

			      START:
				my @lines = ();
				my @rawlines = ();
				my @header_lines = ();
				my @header_rawlines = ();
				my $cmd = runbowtie2(
					$do_build && $first,
					$large_idx,
					$binary_type,
					"$a",
					$tmpfafn,
					$c->{report},
					$read_file_format, # formate of read/mate files
					$read_file,        # read file
					$mate1_file,       # mate #1 file
					$mate2_file,       # mate #2 file
					$reads,            # read list
					$quals,            # quality list
					$m1s,              # mate #1 sequence list
					$q1s,              # mate #1 quality list
					$m2s,              # mate #2 sequence list
					$q2s,              # mate #2 quality list
					$c->{names},
                                        $test_bam,
                                        $test_sra,
					\$has_poison_reads,
					\@lines,
					\@rawlines,
					\@header_lines,
					\@header_rawlines,
					$c->{should_abort});

				if (defined($c->{fastq}) || defined($c->{fastq1}) || !defined($read_file_format)) {
					$reads_are_fastq = 1;
				}

				$first = 0;
				my $pe = defined($c->{mate1s}) && $c->{mate1s} ne "";
				$pe = $pe || defined($mate1_file);
				$pe = $pe || $c->{paired};
				my ($lastchr, $lastoff, $lastoff_orig) = ("", -1, -1);
				# Keep temporary copies of hits and pairhits so that we can
				# restore for the next orientation
				my $hitstmp = [];
				$hitstmp = clone($c->{hits}) if defined($c->{hits});
				my $pairhitstmp = [];
				$pairhitstmp = clone($c->{pairhits}) if defined($c->{pairhits});
				my $pairhits_orig_tmp = [];
				$pairhits_orig_tmp = clone($c->{pairhits_orig}) if defined($c->{pairhits_orig});
				# Record map from already-seen read name, read sequence and
				# quality to the place on the reference where it's reported.
				# This allows us to check that the pseudo-random generator
				# isn't mistakenly yielding different alignments for identical
				# reads.
				my %seenNameSeqQual = ();
				if(defined($c->{lines})) {
					my $l = scalar(@lines);
					$l == $c->{lines} || die "Expected $c->{lines} lines, got $l";
				}
				for my $li (0 .. scalar(@lines)-1) {
					my $l = $lines[$li];
					my ($readname, $orient, $chr, $off_orig, $off, $seq, $qual, $mapq,
						$oms, $editstr, $flagstr, $samflags, $cigar, $rnext, $pnext,
						$tlen);
					my %samoptflags = ();
					if($sam) {
						scalar(@$l) >= 11 ||
							die "Bad number of fields; expected at least 11 got ".
								scalar(@$l).":\n$rawlines[$li]\n";
						($readname, $samflags, $chr, $off) = @$l[0..3];
						($seq, $qual) = @$l[9..10];
						$orient = ((($samflags >> 4) & 1) == 0) ? "+" : "-";
						$mapq  = $l->[4]; # mapping quality
						$cigar = $l->[5]; # CIGAR string
						$rnext = $l->[6]; # ref seq of next frag in template
						$pnext = $l->[7]; # position of next frag in template
						$tlen  = $l->[8]; # template length
						if($pnext == 0) { $pnext = "*"; } else { $pnext--; }
						for(my $m = 11; $m < scalar(@$l); $m++) {
							next if $l->[$m] eq "";
							my ($nm, $ty, $vl) = split(/:/, $l->[$m]);
							defined($vl) ||
								die "Could not parse optional flag field $m: ".
									"\"$l->[$m]\"";
							$samoptflags{$nm}{ty} = $ty;
							$samoptflags{$nm}{vl} = $vl;
						}
						if($off > 0) { $off--; }
						else { $off = "*"; }
						$off_orig = $off;
						$off = "*" if $cigar eq "*";
					} else {
						scalar(@$l) == 9 ||
							die "Bad number of fields; expected 9 got ".
								scalar(@$l).":\n$rawlines[$li]\n";
						($readname, $orient, $chr, $off, $seq,
						 $qual, $oms, $editstr, $flagstr) = @$l;
						$off_orig = $off;
					}
					if($c->{check_random}) {
						my $rsqKey = "$readname\t$orient\t$seq\t$qual";
						my $rsqVal = "$chr\t$off";
						if(defined($seenNameSeqQual{$rsqKey})) {
							$seenNameSeqQual{$rsqKey} eq $rsqVal ||
								die "Two hits for read/seq/qual:\n$rsqKey\n".
									"had different alignments:\n".
									"$seenNameSeqQual{$rsqKey}\n$rsqVal\n";
						}
						$seenNameSeqQual{$rsqKey} = $rsqVal;
					}
					$readname ne "" || die "readname was blank:\n".Dumper($c);
					my $rdi = $readname;
					if ($test_sra and $readname =~ /^\.test_sra/) {
						$rdi = substr($rdi, -1) - 1;
					} else {
						$rdi = substr($rdi, 1) if substr($rdi, 0, 1) eq "r";
					}
					my $mate = 0;
					if($readname =~ /\//) {
						($rdi, $mate) = split(/\//, $readname);
						defined($rdi) || die;
					}
					$rdi = $c->{idx_map}{$rdi} if defined($c->{idx_map}{$rdi});
					$rdi ne "" || die "rdi was blank:\nreadname=$readname\n".Dumper($c);
					if($rdi != int($rdi)) {
						# Read name has non-numeric characters.  Figure out
						# what number it is by scanning the names list.
						my $found = 0;
						for(my $i = 0; $i < scalar(@{$c->{names}}); $i++) {
							if($c->{names}->[$i] eq $readname) {
								$rdi = $i;
								$found = 1;
								last;
							}
						}
						$found || die "No specified name matched reported name $readname";
					}
					# Make simply-named copies of some portions of the test case
					# 'hits'
					my %hits = ();
					%hits = %{$c->{hits}->[$rdi]} if
						defined($c->{hits}->[$rdi]);
					# 'flags'
					my $flags = undef;
					$flags = $c->{flags}->[$rdi] if
						defined($c->{flags}->[$rdi]);
					# 'samflags'
					my $ex_samflags = undef;
					$ex_samflags = $c->{ex_samflags}->[$rdi] if
						defined($c->{ex_samflags}->[$rdi]);
					# 'samflags_map'
					my $ex_samflags_map = undef;
					$ex_samflags_map = $c->{samflags_map}->[$rdi] if
						defined($c->{samflags_map}->[$rdi]);
					# 'samoptflags'
					my $ex_samoptflags = undef;
					$ex_samoptflags = $c->{samoptflags}->[$rdi] if
						defined($c->{samoptflags}->[$rdi]);
					# 'cigar'
					my $ex_cigar = undef;
					$ex_cigar = $c->{cigar}->[$rdi] if
						defined($c->{cigar}->[$rdi]);
					# 'cigar_map'
					my $ex_cigar_map = undef;
					$ex_cigar_map = $c->{cigar_map}->[$rdi] if
						defined($c->{cigar_map}->[$rdi]);
					# 'mapq_hi' - boolean indicating whether mapq is hi/lo
					my $ex_mapq_hi = undef;
					$ex_mapq_hi = $c->{mapq_hi}->[$rdi] if
						defined($c->{mapq_hi}->[$rdi]);
					# 'mapq'
					my $ex_mapq = undef;
					$ex_mapq = $c->{mapq}->[$rdi] if
						defined($c->{mapq}->[$rdi]);
					# 'mapq_map'
					my $ex_mapq_map = undef;
					$ex_mapq_map = $c->{mapq_map}->[$rdi] if
						defined($c->{mapq_map}->[$rdi]);
					# 'rnext_map'
					my $ex_rnext_map = undef;
					$ex_rnext_map = $c->{rnext_map}->[$rdi] if
						defined($c->{rnext_map}) && defined($c->{rnext_map}->[$rdi]);
					# 'pnext_map'
					my $ex_pnext_map = undef;
					$ex_pnext_map = $c->{pnext_map}->[$rdi] if
						defined($c->{pnext_map}) && defined($c->{pnext_map}->[$rdi]);
					# 'tlen_map'
					my $ex_tlen_map = undef;
					$ex_tlen_map = $c->{tlen_map}->[$rdi] if
						defined($c->{tlen_map}) && defined($c->{tlen_map}->[$rdi]);
					# 'flags_fw'
					my $flags_fw = undef;
					$flags_fw = $c->{flags_fw}->[$rdi] if
						defined($c->{flags_fw}->[$rdi]);
					# 'flags_rc'
					my $flags_rc = undef;
					$flags_rc = $c->{flags_rc}->[$rdi] if
						defined($c->{flags_rc}->[$rdi]);
					# 'pairhits'
					my %pairhits = ();
					%pairhits = %{$c->{pairhits}->[$rdi]} if
						defined($c->{pairhits}->[$rdi]);
					# 'pairhits_orig'
					my %pairhits_orig = ();
					%pairhits_orig = %{$c->{pairhits_orig}->[$rdi]} if
						defined($c->{pairhits_orig}->[$rdi]);
					# 'pairflags'
					my %pairflags = ();
					%pairflags = %{$c->{pairflags}->[$rdi]} if
						defined($c->{pairflags}->[$rdi]);
					# 'hits_are_superset'
					my $hits_are_superset = 0;
					$hits_are_superset = $c->{hits_are_superset}->[$rdi] if
						defined($ci);
					# edits
					my $ex_edits = undef;
					$ex_edits = $c->{edits}->[$rdi] if
						defined($c->{edits}->[$rdi]);
					if(!$sam) {
						# Bowtie flags
						if(defined($flags)) {
							$flagstr eq $flags ||
								die "Expected flags=\"$flags\", got \"$flagstr\"";
						}
						if(defined($flags_fw) && $fw) {
							$flagstr eq $flags_fw ||
								die "Expected flags=\"$flags_fw\", got \"$flagstr\"";
						}
						if(defined($flags_rc) && !$fw) {
							$flagstr eq $flags_rc ||
								die "Expected flags=\"$flags_rc\", got \"$flagstr\"";
						}
						if(defined($c->{flag_map})) {
							if(defined($c->{flag_map}->[$rdi]->{$off})) {
								$flagstr eq $c->{flag_map}->[$rdi]->{$off} ||
									die "Expected flags=\"$c->{flag_map}->[$rdi]->{$off}\"".
										" at offset $off, got \"$flagstr\"";
							}
						}
					}
					if($sam) {
						# SAM flags
						if(defined($ex_samflags)) {
							$samflags eq $ex_samflags ||
								die "Expected flags $ex_samflags, got $samflags";
						}
						if(defined($ex_samflags_map)) {
							if(defined($c->{samflags_map}->[$rdi]->{$off})) {
								my $ex = $c->{samflags_map}->[$rdi]->{$off};
                                                                if (ref $ex eq "ARRAY") {
                                                                        $ex = $ex->[$off == $lastoff];
                                                                }
								$samflags eq $ex || die
									"Expected FLAGS value $ex at offset $off, got $samflags"
							} else {
								die "Expected to see alignment with offset $off parsing samflags_map";
							}
						}
						# CIGAR string
						if(defined($ex_cigar)) {
							$cigar eq $ex_cigar ||
								die "Expected CIGAR string $ex_cigar, got $cigar";
						}
						if(defined($ex_cigar_map)) {
							if(defined($c->{cigar_map}->[$rdi]->{$off})) {
								my $ex = $c->{cigar_map}->[$rdi]->{$off};
								$cigar eq $ex || die
									"Expected CIGAR string $ex at offset $off, got $cigar"
							} else {
								die "Expected to see alignment with offset $off parsing cigar_map";
							}
						}
						# MAPQ
						if(defined($ex_mapq)) {
							$mapq eq $ex_mapq ||
								die "Expected MAPQ $ex_mapq, got $mapq";
						}
						if(defined($ex_mapq_map)) {
							if(defined($c->{mapq_map}->[$rdi]->{$off})) {
								my $ex = $c->{mapq_map}->[$rdi]->{$off};
								$mapq eq $ex || die
									"Expected MAPQ string $ex at offset $off, got $mapq"
							} else {
								die "Expected to see alignment with offset $off parsing mapq_map";
							}
						}
						# MAPQ
						if(defined($ex_mapq_hi)) {
							if($ex_mapq_hi == 0) {
								$mapq < 20 || die "Expected MAPQ < 20, got $mapq";
							} else {
								$mapq >= 20 || die "Expected MAPQ >= 20, got $mapq";
							}
						}
						if(defined($ex_mapq_map)) {
							if(defined($c->{mapq_map}->[$rdi]->{$off})) {
								my $ex = $c->{mapq_map}->[$rdi]->{$off};
								$mapq eq $ex || die
									"Expected MAPQ string $ex at offset $off, got $mapq"
							} else {
								die "Expected to see alignment with offset $off parsing mapq_map";
							}
						}
						# SAM optional flags
						if(defined($ex_samoptflags)) {
							matchSamOptionalFlags(\%samoptflags, $ex_samoptflags);
						}
						if(defined($c->{samoptflags_map})) {
							if(defined($c->{samoptflags_map}->[$rdi]->{$off})) {
								matchSamOptionalFlags(
									\%samoptflags,
									$c->{samoptflags_map}->[$rdi]->{$off});
							} else {
								die "Expected to see alignment with offset $off parsing samoptflags_map";
							}
						}
						if(defined($c->{samoptflags_flagmap})) {
							if(defined($c->{samoptflags_flagmap}->[$rdi]->{$samflags})) {
								matchSamOptionalFlags(
									\%samoptflags,
									$c->{samoptflags_flagmap}->[$rdi]->{$samflags});
							} else {
								die "Expected to see alignment with flag $samflags parsing samoptflags_flagmap";
							}
						}
						# RNEXT map
						if(defined($c->{rnext_map})) {
							if(defined($c->{rnext_map}->[$rdi]->{$off})) {
								my $ex = $c->{rnext_map}->[$rdi]->{$off};
								$rnext eq $ex || die
									"Expected RNEXT '$ex' at offset $off, got '$rnext'"
							} else {
								die "Expected to see alignment with offset $off parsing rnext_map".Dumper($c);
							}
						}
						# PNEXT map
						if(defined($c->{pnext_map})) {
							if(defined($c->{pnext_map}->[$rdi]->{$off})) {
								my $ex = $c->{pnext_map}->[$rdi]->{$off};
								$pnext eq $ex || die
									"Expected PNEXT '$ex' at offset $off, got '$pnext'"
							} else {
								die "Expected to see alignment with offset $off parsing pnext_map";
							}
						}
						# TLEN map
						if(defined($c->{tlen_map})) {
							if(defined($c->{tlen_map}->[$rdi]->{$off})) {
								my $ex = $c->{tlen_map}->[$rdi]->{$off};
                                                                if (ref $ex eq "ARRAY") {
                                                                        $ex = $ex->[$off == $lastoff];
                                                                }
								$tlen eq $ex || die
									"Expected TLEN '$ex' at offset $off, got '$tlen'"
							} else {
								die "Expected to see alignment with offset $off parsing tlen_map";
							}
						}
					}
					if($pe && $lastchr ne "") {
						my $offkey_orig = $lastoff.",".$off_orig;
						$offkey_orig = $off_orig.",".$lastoff_orig if $off_orig eq "*";

						my $offkey = $lastoff.",".$off;
						$offkey = $off.",".$lastoff if $off eq "*";

						if($lastoff ne "*" && $off ne "*") {
							$offkey = min($lastoff, $off).",".max($lastoff, $off);
						}
						if(defined($c->{pairhits}->[$rdi])) {
							defined($pairhits{$offkey}) ||
								die "No such paired off as $offkey in pairhits list: ".Dumper(\%pairhits)."\n";
							$c->{pairhits}->[$rdi]->{$offkey}--;
							delete $c->{pairhits}->[$rdi]->{$offkey} if $c->{pairhits}->[$rdi]->{$offkey} == 0;
							%pairhits = %{$c->{pairhits}->[$rdi]};
						}
						if(defined($c->{pairhits_orig}->[$rdi])) {
							defined($pairhits_orig{$offkey_orig}) ||
								die "No such paired off as $offkey in pairhits_orig list: ".Dumper(\%pairhits_orig)."\n";
							$c->{pairhits_orig}->[$rdi]->{$offkey_orig}--;
							delete $c->{pairhits_orig}->[$rdi]->{$offkey_orig} if $c->{pairhits_orig}->[$rdi]->{$offkey_orig} == 0;
							%pairhits_orig = %{$c->{pairhits_orig}->[$rdi]};
						}
						($lastchr, $lastoff, $lastoff_orig) = ("", -1, -1);
					} elsif($pe) {
						# Found an unpaired alignment from aligning a pair
						my $foundSe =
							defined($c->{pairhits}->[$rdi]) &&
							$c->{pairhits}->[$rdi]->{$off};
						if($foundSe) {
							$c->{pairhits}->[$rdi]->{$off}--;
							delete $c->{pairhits}->[$rdi]->{$off}
								if $c->{pairhits}->[$rdi]->{$off} == 0;
							%pairhits = %{$c->{pairhits}->[$rdi]};
						} else {
							($lastchr, $lastoff) = ($chr, $off);
						}
						# Found an unpaired alignment from aligning a pair
						$foundSe =
							defined($c->{pairhits_orig}->[$rdi]) &&
							$c->{pairhits_orig}->[$rdi]->{$off_orig};
						if($foundSe) {
							$c->{pairhits_orig}->[$rdi]->{$off_orig}--;
							delete $c->{pairhits_orig}->[$rdi]->{$off_orig}
								if $c->{pairhits_orig}->[$rdi]->{$off_orig} == 0;
							%pairhits_orig = %{$c->{pairhits_orig}->[$rdi]};
						} else {
							($lastchr, $lastoff, $lastoff_orig) = ($chr, $off, $off_orig);
						}
					} else {
						if(defined($c->{hits}->[$rdi])) {
							defined($hits{$off}) ||
								die "No such off as $off in hits list: ".Dumper(\%hits)."\n";
							$c->{hits}->[$rdi]->{$off}--;
							delete $c->{hits}->[$rdi]->{$off} if $c->{hits}->[$rdi]->{$off} == 0;
							%hits = %{$c->{hits}->[$rdi]};
						}
					}
					if(!$sam && defined($ex_edits)) {
						my $eds = $l->[7];
						$eds eq $ex_edits ||
							die "For edit string, expected \"$ex_edits\" got \"$eds\"\n";
					}
				}

				# Go through all the per-read
				my $klim = 0;
				$klim = scalar(@{$c->{hits}}) if defined($c->{hits});
				$klim = max($klim, scalar(@{$c->{pairhits}})) if defined($c->{pairhits});
				for (my $k = 0; $k < $klim; $k++) {
					# For each read
					my %hits     = %{$c->{hits}->[$k]}     if defined($c->{hits}->[$k]);
					my %pairhits = %{$c->{pairhits}->[$k]} if defined($c->{pairhits}->[$k]);
					my %pairhits_orig = %{$c->{pairhits_orig}->[$k]} if defined($c->{pairhits_orig}->[$k]);
					my $hits_are_superset = $c->{hits_are_superset}->[$k];
					# Check if there are any hits left over
					my $hitsLeft = scalar(keys %hits);
					if($hitsLeft != 0 && !$hits_are_superset) {
						print Dumper(\%hits);
						die "Had $hitsLeft hit(s) left over at position $k";
					}
					my $pairhitsLeft = scalar(keys %pairhits);
					if($pairhitsLeft != 0 && !$hits_are_superset) {
						print Dumper(\%pairhits);
						die "Had $pairhitsLeft hit(s) left over at position $k";
					}
					my $pairhits_orig_Left = scalar(keys %pairhits_orig);
					if($pairhits_orig_Left != 0 && !$hits_are_superset) {
						print Dumper(\%pairhits_orig);
						die "Had $pairhits_orig_Left hit(s) left over at position $k";
					}
				}

				$c->{hits} = $hitstmp;
				$c->{pairhits} = $pairhitstmp;
				$c->{pairhits_orig} = $pairhits_orig_tmp;


                                if ($should_test_bam && !$c->{should_abort} && $reads_are_fastq && !$has_poison_reads && !$test_bam) {
                                        $test_bam = 1;
                                        goto START;
                                }

				if ($compiled_with_sra && $reads_are_fastq && !$has_poison_reads && !$test_sra) {
					$test_sra = 1;
					goto START;
				}
			}
			$last_ref = undef if $first;
		}
    }
}
print "PASSED\n";
