---
title: "metaseqR<sup>2</sup> report"
subtitle: "`r basename(PROJECT_PATH$main)`"
date: "`r format(Sys.time(), '%d/%m/%Y')`"
output:
  rmdformats::material:
    pandoc_args: ["+RTS","-K2048m","-RTS"]
    fig_width: 7
    fig_height: 7
    fig_caption: true
    highlight: kate
    lightbox: true
    thumbnails: true
    gallery: true
    cards: true
    use_bookdown: false
    mathjax: null
    self_contained: false
---

```{r knitr_init, echo=FALSE, cache=FALSE}
library(knitr)
library(rmdformats)

# Global options
options(max.print="75")
opts_chunk$set(
    echo=FALSE,
    cache=FALSE,
    prompt=FALSE,
    tidy=TRUE,
    comment=NA,
    message=FALSE,
    warning=FALSE,
    results="asis",
    eval=TRUE
)
opts_knit$set(width=75)
```

```{r load_js}
if (offlineReport) {
    #cat("<script type=\"text/javascript\" src=\"js/pace.min.js\"></script>",
    #   sep="")
    cat("<script type=\"text/javascript\" src=\"js/highcharts.js\"></script>",
        sep="")
    cat("<script type=\"text/javascript\" src=\"js/highcharts-more.js\">",
        "</script>",sep="")
    cat("<script type=\"text/javascript\" src=\"js/exporting.js\"></script>",
        sep="")
    cat("<script type=\"text/javascript\" src=\"js/offline-exporting.js\">",
        "</script>",sep="")
    cat("<script type=\"text/javascript\" src=\"js/export-data.js\"></script>",
        sep="")
    cat("<script type=\"text/javascript\" src=\"js/canvas2svg.js\"></script>",
        sep="")
    cat("<script type=\"text/javascript\" src=\"js/jvenn.min.js\"></script>",
        sep="")
    if (reportDb == "sqlite") {
        cat("<script>Module = { TOTAL_MEMORY: 134217728 };</script>")
        cat("<script type=\"text/javascript\" src=\"js/sql.js\"></script>",
            sep="")
    } else if (reportDb == "dexie") {
       cat("<script type=\"text/javascript\" src=\"js/dexie.min.js\"></script>",
            sep="")
    }
} else {
    #cat("<script type=\"text/javascript\" src=\"https://raw.github.com/",
    #   "HubSpot/pace/v1.0.0/pace.min.js\"></script>",sep="")
    cat("<script type=\"text/javascript\" src=\"https://code.highcharts.com/",
        "highcharts.js\"></script>",sep="")
    cat("<script type=\"text/javascript\" src=\"https://code.highcharts.com/",
        "highcharts-more.js\"></script>",sep="")
    cat("<script type=\"text/javascript\" src=\"https://code.highcharts.com/",
        "modules/exporting.js\"></script>",sep="")
    cat("<script type=\"text/javascript\" src=\"https://code.highcharts.com/",
        "modules/offline-exporting.js\"></script>",sep="")
    cat("<script type=\"text/javascript\" src=\"https://code.highcharts.com/",
        "modules/export-data.js\"></script>",sep="")
    cat("<script type=\"text/javascript\ ",
        "src=\"http://jvenn.toulouse.inra.fr/app/js/canvas2svg.js\">",
        "</script>",sep="")
    cat("<script type=\"text/javascript\" ",
        "src=\"http://jvenn.toulouse.inra.fr/app/js/jvenn.min.js\">",
        "</script>",sep="")
    if (reportDb == "sqlite") {
        cat("<script>Module = { TOTAL_MEMORY: 134217728 };</script>")
        cat("<script type=\"text/javascript\" src=\"",
        "https://cdnjs.cloudflare.com/ajax/libs/sql.js/0.5.0/js/sql.js\">",
        "</script>",sep="")
    } else if (reportDb == "dexie") {
        cat("<script type=\"text/javascript\" ",
            "src=\"https://unpkg.com/dexie@2.0.4/dist/dexie.min.js\">",
            "</script>",sep="")
    }
}
if (reportDb == "dexie")
    cat("<script type=\"text/javascript\" src=\"data/reportdb.js\"></script>")
```

```{js pace_opts}
Pace.options = {
    ajax: false,
    restartOnPushState: false,
    restartOnRequestAfter: false
}
```

<div id="loader" class="loading"></div>

```{r decide_dexie}
if (reportDb == "dexie") {
    code <- paste0(
        "var DEXIE = true;\n",
        "var db = new Dexie('plotdb');\n",
        "db.version(1).stores({\n",
        "    plots: '++_id,[name+type+subtype]'\n",
        "});\n",
        "db.plots.clear().then(console.log('Previous plots cleared!'));\n",
        "db.plots.bulkAdd(plotData);"
    )
} else if (reportDb == "sqlite") {
    code <- "var DEXIE = false;"
}
cat("<script>\n",code,"\n</script>")
```

```{js define_getplot_funs}
getPlotFromDb = function(query,renderId) {
    if (DEXIE) {
        $("#loader").show();
        db.plots.get({
            name: query.name,
            type: query.type,
            subtype: query.subtype
        },function(res) {
            var chartData = res.json;
            chartData.chart.renderTo = renderId;
            chartData.chart.events = {
                load: function() {
                    $("#loader").hide();
                }
            };
            var chart = new Highcharts.Chart(chartData);
        });
    }
    else {
        var xhr = new XMLHttpRequest();
        xhr.open('GET', './data/reportdb.sqlite',true);
        xhr.responseType = 'arraybuffer';
        xhr.onload = function(e) {
            var sqlQuery = "SELECT json FROM plot WHERE name='" + query.name + 
                "'" +" AND type='" + query.type + "'" + " AND subtype='" + 
                query.subtype + "';";
            var uInt8Array = new Uint8Array(this.response);
            var db = new SQL.Database(uInt8Array);
            var contents = db.exec(sqlQuery);
            var chartData = JSON.parse(contents[0].values[0][0]);
            //console.log(chartData)
            chartData.chart.renderTo = renderId;
            chartData.chart.events = {
                load: function() {
                    $("#loader").hide();
                }
            };
            var chart = new Highcharts.Chart(chartData);
        };
        xhr.send();
        $("#loader").show();
    }
}

getPlotWithFunFromDb = function(query,renderId) {
    if (DEXIE) {
        $("#loader").show();
        db.plots.get({
            name: query.name,
            type: query.type,
            subtype: query.subtype
        },function(res) {
            var chartData = res.json;
            chartData.chart.renderTo = renderId;
            
            // Deserialize the stored functions - only in a couple of cases
            if (query.type === "countsbio") {
                chartData.yAxis.labels.formatter = new Function('return ' +
                    chartData.yAxis.labels.formatter)()
                    
                chartData.plotOptions.scatter.tooltip.pointFormatter = 
                    new Function('return ' +
                        chartData.plotOptions.scatter.tooltip.pointFormatter)()
                
                chartData.plotOptions.boxplot.events.legendItemClick = 
                    new Function('return ' + 
                        chartData.plotOptions.boxplot.events.legendItemClick)()
            }
            if (query.type === "boxplot") {
                chartData.plotOptions.boxplot.tooltip.pointFormatter = 
                    new Function('return ' +
                        chartData.plotOptions.boxplot.tooltip.pointFormatter)()
                
                chartData.plotOptions.boxplot.events.legendItemClick = 
                    new Function('return ' + 
                        chartData.plotOptions.boxplot.events.legendItemClick)()
            }
            
            chartData.chart.events = {
                load: function() {
                    $("#loader").hide();
                }
            };
            var chart = new Highcharts.Chart(chartData);
        });
    }
    else {
        var xhr = new XMLHttpRequest();
        xhr.open('GET', './data/reportdb.sqlite',true);
        xhr.responseType = 'arraybuffer';
        xhr.onload = function(e) {
            var sqlQuery = "SELECT json FROM plot WHERE name='" + query.name + 
                "'" + " AND type='" + query.type + "'" + " AND subtype='" + 
                query.subtype + "';";
            var uInt8Array = new Uint8Array(this.response);
            var db = new SQL.Database(uInt8Array);
            var contents = db.exec(sqlQuery);
            var chartData = JSON.parse(contents[0].values[0][0]);
            
            // Deserialize the stored functions - only in a couple of cases
            if (query.type === "countsbio") {
                chartData.yAxis.labels.formatter = new Function('return ' +
                    chartData.yAxis.labels.formatter)()
                    
                chartData.plotOptions.scatter.tooltip.pointFormatter = 
                    new Function('return ' +
                        chartData.plotOptions.scatter.tooltip.pointFormatter)()
                
                chartData.plotOptions.boxplot.events.legendItemClick = 
                    new Function('return ' + 
                        chartData.plotOptions.boxplot.events.legendItemClick)()
            }
            if (query.type === "boxplot") {
                chartData.plotOptions.boxplot.tooltip.pointFormatter = 
                    new Function('return ' +
                        chartData.plotOptions.boxplot.tooltip.pointFormatter)()
                
                chartData.plotOptions.boxplot.events.legendItemClick = 
                    new Function('return ' + 
                        chartData.plotOptions.boxplot.events.legendItemClick)()
            }
            
            chartData.chart.renderTo = renderId;
            chartData.chart.events = {
                load: function() {
                    $("#loader").hide();
                }
            };
            var chart = new Highcharts.Chart(chartData);
        };
        xhr.send();
        $("#loader").show();
    }
}

getVennFromDb = function(query,renderId) {
    if (DEXIE) {
        $("#loader").show();
        db.plots.get({
            name: query.name,
            type: query.type,
            subtype: query.subtype
        },function(res) {
            var chartData = res.json;
            chartData.fnClickCallback = function() {
                var value = '';
                if (this.listnames.length == 1) {
                    value += 'Elements only in ';
                } else {
                    value += 'Common elements in ';
                }
                for (name in this.listnames) {
                    value += this.listnames[name] + ' ';
                }
                value += ':\n';
                for (val in this.list) {
                    value += this.list[val] + '\n';
                }
                if (query.subtype === "stat_dereg" 
                    || query.subtype === "stat_up"
                    || query.subtype === "stat_down") {
                    $('#jvenn_stat_list').text(value);
                }
                if (query.subtype === "fold_dereg" 
                    || query.subtype === "fold_up"
                    || query.subtype === "fold_down") {
                    $('#jvenn_fold_list').text(value);
                }
            }
            $('#'+renderId).jvenn(chartData);
            $("#loader").hide();
        });
    }
    else {
        var xhr = new XMLHttpRequest();
        xhr.open('GET', './data/reportdb.sqlite',true);
        xhr.responseType = 'arraybuffer';
        xhr.onload = function(e) {
            var sqlQuery = "SELECT json FROM plot WHERE name='" + query.name + 
                "'" +" AND type='" + query.type + "'" + " AND subtype='" + 
                query.subtype + "';";
            
            var uInt8Array = new Uint8Array(this.response);
            var db = new SQL.Database(uInt8Array);
            var contents = db.exec(sqlQuery);
            var chartData = JSON.parse(contents[0].values[0][0]);
            chartData.fnClickCallback = function() {
                var value = '';
                if (this.listnames.length == 1) {
                    value += 'Elements only in ';
                } else {
                    value += 'Common elements in ';
                }
                for (name in this.listnames) {
                    value += this.listnames[name] + ' ';
                }
                value += ':\n';
                for (val in this.list) {
                    value += this.list[val] + '\n';
                }
                if (query.subtype === "stat_dereg" 
                    || query.subtype === "stat_up"
                    || query.subtype === "stat_down") {
                    $('#jvenn_stat_list').text(value);
                }
                if (query.subtype === "fold_dereg" 
                    || query.subtype === "fold_up"
                    || query.subtype === "fold_down") {
                    $('#jvenn_fold_list').text(value);
                }
            }
            $('#'+renderId).jvenn(chartData);
            $("#loader").hide();
        };
        xhr.send();
        $("#loader").show();
    }
}
```

<style>
p {
    margin: 0 0 10px;
    text-align: justify;
}

.detable-container {
    font-size: 0.8em;
}

.header-panel {
    background-color: #0E2B8A;
    min-height: 144px;
    position: relative;
    z-index: 3;
}

.header-panel h1.subtitle {
    font-size: 16px;
    font-weight: 600;
}

.figure-hint {
    text-align: justify;
    font-size: 0.9em;
}

.hc-rect-sm {
    width: 640px;
    height: 640px;
    margin: auto;
}

.hc-rect-xs {
    width: 320px;
    height: 320px;
    margin: auto;
}

.hc-selector-label {
    color: #0030CE;
    font-size: 0.9em;
    font-weight: 600;
}

.deheatmap-container {
    width: 740px;
    height: 860px;
    text-align: center;
    margin: 25px auto 5px;
}

.coheatmap-container {
    width: 640px;
    height: 640px;
    text-align: center;
    margin: auto;
}

.link-box {
    background-color: #D5EEEB; 
    border-style: solid; 
    border-color: #00887A; 
    border-width: 1px; 
    width: 80%; 
    padding: 10px;
    margin-top: 20px;
    -moz-border-radius: 5px;
    -webkit-border-radius: 5px;
    -khtml-border-radius: 5px;
    border-radius: 5px;
}

.hidden-element {
    visibility: hidden;
    width: 10px;
    height: 10px;
}

.jvenn_list {
    font-size: 0.9em;
    font-wight: 600;
    width: 100%;
    height: 300px;
}

#summary_log_container {
    font-family: "Courier New", Courier, monospace;
    font-size: 0.9em;
    height: 400px;
    overflow-y: scroll;
    overflow-x: hidden;
    overflow-wrap: normal;
}

/******************************* Chart loading ********************************/
.loading {
  display: none;
  position: fixed;
  z-index: 999;
  height: 100%;
  width: 100%;
  overflow: show;
  margin: auto;
  top: 0;
  left: 0;
  bottom: 0;
  right: 0;
  background: radial-gradient(rgba(76, 51, 204, 0.7), rgba(0, 0, 0, .8));
  background: -webkit-radial-gradient(rgba(76, 51, 204, 0.7), rgba(0, 0, 0,.8));
}

.loading:before {
  content: 'Loading plots...';
  font-size: 92px;
  font-weight: 400;
  color: white;
  display: block;
  position: fixed;
  top: 40%;
  left: 35%;
  width: 100%;
  height: 100%;
}

/********************************** Pace.js ***********************************/
.pace {
  -webkit-pointer-events: none;
  pointer-events: none;

  -webkit-user-select: none;
  -moz-user-select: none;
  user-select: none;
}

.pace.pace-inactive .pace-progress {
  display: none;
}

.pace .pace-progress {
  position: fixed;
  z-index: 2000;
  top: 50%;
  left: 50%;
  height: 240px;
  width: 240px;
  margin-top: -120px; /* Negative half of height. */
  margin-left: -220px; /* Negative half of width. */

  -webkit-transform: translate3d(0, 0, 0) !important;
  -ms-transform: translate3d(0, 0, 0) !important;
  transform: translate3d(0, 0, 0) !important;
}

.pace .pace-progress:after {
  display: block;
  position: absolute;
  top: 0;
  content: attr(data-progress-text);
  font-family: "Helvetica Neue", sans-serif;
  font-weight: 200;
  font-size: 240px;
  line-height: 1;
  text-align: right;
  color: rgba(0, 0, 220, 0.3);
}

.pace .pace-progress:before {
  display: block;
  position: absolute;
  top: 0;
  content: url(media/dna_loader.gif);
  margin-left: -300px;
  margin-top: -120px;
}

.pace-running > *:not(.pace) {
  opacity:0;
}
/******************************** End Pace.js *********************************/
</style>

```{r report_init}
library(DT)
library(GenomicRanges)
library(gplots)
library(heatmaply)
library(htmltools)
library(magrittr)
library(pander)

makeFoldChange <- metaseqR2:::makeFoldChange
nat2log <- metaseqR2:::nat2log
asClassVector <- metaseqR2:::asClassVector
makeReportMessages <- metaseqR2:::makeReportMessages
.formatForReport <- metaseqR2:::.formatForReport

# Initialize some variables used throughout the report
samples <- unlist(sampleList,use.names=FALSE)
biotypes <- unique(as.character(geneData$biotype))
reportMessages <- makeReportMessages("en")

qualPlots <- c("mds","biodetection","countsbio","saturation","readnoise",
    "correl","pairwise","filtered")
normPlots <- c("boxplot","gcbias","lengthbias","meandiff","meanvar","rnacomp")
statPlots <- c("deheatmap","volcano","mastat","biodist","statvenn","foldvenn",
    "deregulogram")

covarsRaw <- covarsStat <- NULL
if (any(qcPlots %in% c("biodetection","countsbio","saturation","rnacomp",
    "readnoise"))) {
    covarsRaw <- list(
        data=geneCounts,
        length=width(geneData),
        gc=as.numeric(geneData$gc_content),
        chromosome=data.frame(
            chromosome=as.character(seqnames(geneData)),
            start=start(geneData),
            end=end(geneData)
        ),
        factors=data.frame(class=asClassVector(sampleList)),
        biotype=as.character(geneData$biotype),
        gene_name=as.character(geneData$gene_name)
    )
}

if ("biodist" %in% qcPlots) {
    covarsStat <- list(
        data=normGenesExpr,
        length=width(geneDataExpr),
        gc=as.numeric(geneDataExpr$gc_content),
        chromosome=data.frame(
            chromosome=as.character(seqnames(geneDataExpr)),
            start=start(geneDataExpr),
            end=end(geneDataExpr)
        ),
        factors=data.frame(class=asClassVector(sampleList)),
        biotype=as.character(geneDataExpr$biotype),
        gene_name=as.character(geneDataExpr$gene_name)
    )
}
```

# Instructions {.tabset .tabset-pills}

Welcome to the **metaseqR2** report! If you are familiar with the metaseqR 
report, then you will find that there are not many differences with respect to 
the presented information. Some diagnostic and exploration plots were added. The 
most notable difference is that __*all*__ plots are interactive. This helps a 
lot with exploration and interpretation but also adds a lot of computational
burden. However, relatively modern systems with recent browser versions should
be capable of rendering all the graphics. The metaseqR2 report has been tested 
with Google Chrome, Mozilla Firefox and Microsoft Edge. It has **not** been 
tested with Internet Explorer, Opera and Safari and most probably will not be. 
Other Chromium browsers (e.g. Brave) should also be fine.

One particular characteristic of the metaseqR2 report is that all plots are
interactive. This is achieved by using the standard graphics underlying data
with libraries including [Highcharts](https://www.highcharts.com/),
[Plotly](https://plot.ly/) and 
[jvenn](http://jvenn.toulouse.inra.fr/app/index.html) to create more 
user-friendly and directly explorable plots. Instructions on the usage of these 
plots follow:

* All plots are interactively **explorable**. This means that if you move your 
mouse **inside** the plot area (a move called *mouse-over*), you can retrieve 
information on each single data point. This applies to all plots. More 
specifically:
  + In **scatterplots**, if you mouse-over each point, information about this 
  point is presented, depending on the type of the plot. The data series from 
  which the point comes is also presented. For example, in a Volcano plot, fold 
  change and significance, as well as the name of the gene and the data category
  (e.g. up-regulated) will be presented.
  + In **barplots**, if you mouse-over each bar, information about this bar is 
  presented, such as the value it represents and the data series from which it
  comes. If the barplot contains groups of bars, then information about each 
  group is displayed. For example, in a Biodetection plot, each bar group
  presents the percentage of a biotype in the examined genome, the percentage
  in the sample and the detected percentage according to read counts.
  + In **boxplots**, if you mouse-over the boxes, the information about the 
  underlying distribution is displayed (maximum, upper quartile, median, lower
  quartile and minimum) as well as the data series. If you mouse-over an 
  outlier, then information on this single point is presented (e.g. value).
  + Some **barplots** have a **double** y-axis system corresponding to 
  **different** measurements or scales. For example in Biodetection barplots,
  the left y-axis presents abundant features while the right y-axis presents
  non-abundant features. In the Filtered barplot, y-axes present different 
  values (numbers and fractions).
  + **Line plots** can be moused-over too. Depending on the plot type, exact 
  values may or may not be shown, depending on how important it is to display 
  them, and to avoid over-crowding the plots. For example in Reads noise plot,
  we are interested in the trend and not so much in exact values.
  + **Heatmaps** can be moused-over too. Information on each heatmap cell will 
  be displayed.
* All scatterplots and heatmaps are **zoomable**. You need to press the left 
mouse button inside a plot area and draw a square area to zoom-in. If you wish 
to reset the zoom, there is a **button** appearing for this when zooming-in.
* Data series in scatterplots, barplots and boxplots can be **toggled** on or 
off by **clicking** on the **legend** name of each data series which is placed 
**below** each plot. For example, in Volcano plots, if you click on the name 
"Unregulated", then the respective data series will stop appearing in the plot. 
You can bring it **back** by clicking the legend again.
* All plots are **exportable**. On the **top right** corner of each scatterplot, 
barplot and boxplot, there is a menu button with several functionalities, 
including **exporting** in various formats and presenting the plot in 
**full-screen** mode. For heatmaps, this functionality is offered by a set of 
small buttons that appear if you mouse-over at the **top** of the heatmap.
* In Venn diagrams, if you **click** on the **number** for each category, the 
respective gene/transcript names will appear in the **box** on the right of the 
diagram.
* All plots can be **downloaded** in static formats (in formats according to 
```metaseqr2``` call) from the Results section.

The metaseqR2 report contains the sections described below, depending on which 
diagnostic and exploration plots have been asked for from the run command. As 
plots are categorized, if no plot from a specific category is asked for, then 
this category will not appear. Below, are the categories:

## Summary

The **Summary** section is further categorized in several subsections. 
Specifically:

* **Analysis summary:** This section contains an auto-generated text that 
analytically describes the computational process followed and summarized the
results of each step. This text can be used as is or with slight modifications
in the <u>Methods</u> section of an article.
* **Input options:** This section provides a list of the input arguments to the
pipeline in a more human-readable format.
* **Filtering:** This section reports in detail the number of filtered genes
decomposed according to the number of genes removed by each applied filter.
* **Differential expression:** This section reports in detail the number of
differentially expressed genes for each contrast, both when using only a p-value
cutoff as well as an FDR cutoff (numbers in parentheses), that is, genes passing
the multiple testing correction procedure selected. These numbers are also
calculated based on a simple fold change cutoff in log<sub>2</sub> scale. 
Finally, when multiple algorithms are used with p-value combination, this
section reports all the findings analytically per algorithm.
* **Command:** This section contains the command used to run the ```metaseqr2```
pipeline for users that want to experiment as well as a critical messages
displayed within the ```R``` session running ```metaseqr2``` displayed as a log.
Finally, if a targets file has been used to perform the analysis, a table
depicting the parameters in the targets files is created and a link to download
the actual targets file, but any relative paths to BAM files are stripped and
the user is responsible to prepend them if the targets file has to be reused in
another location, e.g. locally.
* **Tracks:** This section contains a link which opens a new window to the UCSC
Genome Browser where normalized tracks based on the input BAM files are 
displayed. If stranded tracks have been requested (according to the
sequencing protocol or technology), the a track hub is created to display the
stranded tracks. From this tab, you can also download bigWig files as well as 
copy track lines for manual input to the UCSC Genome Browser.

## Quality control 

The **Quality** control section contains several interactive plots concerning 
the overall quality control of each sample provided as well as overall 
assessments. The quality control plots are the *Multidimensional Scaling* 
(**MDS**) plot, the *Biotypes detection* (**Biodetection**) plot, the 
*Biotype abundance* (**Countsbio**) plot, the *Read saturation* (**Saturation**) 
plot, the *Read noise* (**ReadNoise**) plot, the *Correlation heatmap* 
(**Correlation**), the *Pairwise sample scatterplots* (**Pairwise**) and the 
*Filtered entities* (**Filtered**) plot. Each plot is accompanied by a detailed
description of what it depicts. Where multiple plot are available (e.g. one for
each sample), a selection list on the top of the respective  section allows the
selection of the sample to be displayed.

## Normalization

The **Normalization** section contains several interactive plots that can be 
used to inspect and assess the normalization procedure. Therefore, normalization
plots are usually paired, showing the same data instance normalized and not
normalized. The normalization plots are the *Expression boxplots* (**Boxplots**)
plots, the *GC content bias* (**GC bias**) plots, the *Gene length bias* 
(**Length bias**) plots, the *Within condition mean-difference*
(**Mean-Difference**) plots, the *Mean-variance relationship* 
(**Mean-Variance**) plot and the *RNA composition* (**Rna composition**) plot.
Each plot is accompanied by a detailed description of what it depicts. Where 
multiple plot are available (e.g. one for each sample), a selection list on the 
top of the respective section allows the selection of the sample to be 
displayed.

## Statistics

The **Statistics** section contains several interactive plots that can be used
to inspect and explore the outcome of statistical testing procedures. The 
statistics plots are the *Volcano plot* (**Volcano**), the 
*MA or Mean-Difference across conditions* (**MA**) plot, the 
*Expression heatmap* (**Heatmap**) plot, the 
*Chromosome and biotype distributions* (**Biodist**) plot, the
*Venn diagram across statistical tests* (**StatVenn**), the 
*Venn diagram across contrasts* (**FoldVenn**) and the *Deregulogram*. Each plot 
is accompanied by a detailed description of what it depicts. Please note that 
the heatmap plots only show the top percentage of differentially expressed genes 
as this is controlled by the ```reportTop``` parameter of the ```metaseqr2``` 
pipeline. When multiple plots are available (e.g. one for each contrast), a 
selection list on the top of the respective section allows the selection of the 
sample to be displayed.

## Results

The **Results** section contains a snapshot of differentially expressed genes in 
table format with basic information about each gene and links to external 
resources. Certain columns of the table are colored according to significance. 
Larger bars and more intense colors indicate higher significance. For example, 
the bar in the *p_value* column is larger if the genes has higher statistical 
significance and the fold change cell background is bright red if the gene is 
highly up-regulated. From the **Results** section, full gene lists can be 
downloaded in text tab-delimited format and viewed with a spreadsheet
application such as MS Excel. A selector on the top of the section above the 
table allows the display of different contrasts.

## References

The **References** section contains bibliographical references regading the
algorithms used by the ```metaseqr2``` pipeline and is adjusted according to the
algorithms selected.
 
# Summary {.tabset .tabset-pills}

## Analysis summary

### Analysis summary

```{r summary_analysis_summary}
if (fromRaw) {
    cat("The raw ",fileType," files, one for each RNA-Seq sample, were ",
        "summarized to ")
    if (transLevel == "gene") {
        if (countType=="exon") {
            cat("an exon ") 
        } else if (countType=="gene") {
            cat("a gene ")
        } else if (countType=="utr") {
            cat("a 3'UTR ")
        }
    } else if (transLevel=="transcript") {
        if (countType=="exon") {
            cat("an exon ")
        } else if (countType=="utr") {
            cat("a 3'UTR ")
        }
    } else if (transLevel=="exon") {
        if (countType=="exon")
            cat("an exon ")
    }
    cat("read counts table, ")
    if (fileType=="bam" || fileType=="sam") {
        cat("using the Bioconductor package GenomicRanges. ")
    } else if (fileType=="bed") {
        cat("using the Bioconductor packages rtracklayer and GenomicRanges. ")
    }
    cat("In the final read counts table, each row represented ")
    if (countType=="exon") {
        cat("one exon, ") 
    } else if (countType=="gene") {
        cat("one gene, ")
    }
    cat("each column one RNA-Seq sample and each cell, the corresponding ",
        "read counts associated with each row and column.")
}

if (countType == "exon") {
    if (!is.null(exonFilters)) {
        cat("The exon read counts were filtered for artifacts that could",
            "affect the subsequent normalization and statistical testing",
            "procedures as follows: ")
        msgExonFiltersMinActiveExons <- NULL
        if (!is.null(exonFilters$minActiveExons)) {
            msgExonFiltersMinActiveExons <- paste(
                "if an annotated ",transLevel," had up to ", 
                exonFilters$minActiveExons$exonsPerGene," exons, read ",
                "presence was required in at least ",
                exonFilters$minActiveExons$minExons," of the exons, else if ", 
                "an annotated ",transLevel," had more than ",
                exonFilters$minActiveExons$exonsPerGene," exons, then read ",
                "presence was required in at least ",
                exonFilters$minActiveExons$frac,
                "x&lceil;E&rceil; exons, where &lceil;<sup>.</sup>&rceil; is ",
                "the <em>ceiling</em> mathematical function. The application ",
                "of this filter resulted in the exclusion of ",
                length(exonFilterResult$minActiveExons)," ",transLevel,
                "s from further analysis. ",sep=""
            )
        }
        cat(msgExonFiltersMinActiveExons,sep=", ")
        cat("The total number of ",transLevel,"s excluded due to the ",
            "application of exon filters was ",
             sum(as.numeric(sapply(exonFilterResult,length))),". ",sep="")
    }
    cat("The final read counts for each ",transLevel," model were calculated",
        "as the sums of their exon reads, creating a ",transLevel," counts",
        "table where each row corresponded to an Ensembl",transLevel,
        " model and each column corresponded to an RNA-Seq sample. ")
}

cat("The ",transLevel," counts table was normalized for inherent systematic ",
    "or experimental biases (e.g. sequencing depth, ",transLevel," length, ",
    "GC content bias etc. using the Bioconductor package ",
     reportMessages$norm[[normalization]]," after removing ",transLevel,
    "s that had zero counts over all the RNA-Seq samples (",length(theZeros),
     " ",transLevel,"s). The output of the normalization algorithm ",sep="")
cat("was a table with normalized counts, which can be used for differential",
    "expression analysis with statistical algorithms developed specifically",
    "for count data. ")
    
if (!is.null(geneFilters)) {
    cat("Prior to the statistical testing procedure, the ",transLevel," read ",
    "counts were filtered for possible artifacts that may affect the ",
    "subsequent statistical testing procedures. Genes/transcripts presenting ",
    "any of the following were excluded from further analysis: ")
    
    latinNumbered <- c("i)","ii)","iii)","iv)","v)","vi)","vii)","viii)","ix)",
        "x)","xi)","xii)","xiii)","xiv)","xv)")
    counter <- 0
    msgGeneFiltersLength <- msgGeneFiltersExpressionMedian <- 
        msgGeneFiltersExpressionMean <- msgGeneFiltersExpressionQuantile <-
        msgGeneFiltersExpressionKnown <- msgGeneFiltersExpressionCustom <-
        msgGeneFiltersBiotype <- msgGeneFiltersAvgReads <- 
        msgGeneFiltersPresence <- NULL
    if (!is.null(geneFilters$length)) {
        counter <- counter + 1
        msgGeneFiltersLength <- paste(
            latinNumbered[counter]," ",transLevel,"s with length less than ", 
            geneFilters$length$length,"bp (",length(geneFilterResult$length),
            " genes)",sep=""
        )
    }
    if (!is.null(geneFilters$avgReads)) {
        counter <- counter + 1
        msgGeneFiltersAvgReads <- paste(
            latinNumbered[counter]," ",transLevel,"s whose average numbers of ",
            "reads per ",geneFilters$avgReads$averagePerBp," bp was less than ",
            "the ",100*geneFilters$avgReads$quantile,"<sup>th</sup> quantile ",
            "of the total normalized distribution of average reads per ",
            geneFilters$avgReads$averagePerBp,"bp (",
            length(geneFilterResult$averagePerBp)," ",transLevel,"s with ",
            "cutoff value ",round(geneFilterCutoff$avgReads,digits=5)," ",
            "average reads per ",geneFilters$avgReads$averagePerBp," bp)",sep=""
        )
    }
    if (!is.null(geneFilters$expression)) {
        if (!is.null(geneFilters$expression$median)
            && geneFilters$expression$median) {
            counter <- counter + 1
            msgGeneFiltersExpressionMedian <- paste(
                latinNumbered[counter]," ",transLevel,"s with read counts ",
                "below the median read counts of the total normalized count ",
                "distribution (",length(geneFilterResult$expression$median)," ",
                transLevel,"s with cutoff value ",
                geneFilterCutoff$expression$median," normalized read counts)",
                sep=""
            )
        }
        if (!is.null(geneFilters$expression$mean) 
            && geneFilters$expression$mean) {
            counter <- counter + 1
            msgGeneFiltersExpressionMean <- paste(
                latinNumbered[counter]," ",transLevel,"s with read counts ",
                "below the mean read counts of the total normalized counts ",
                "distribution (",length(geneFilterResult$expression$mean)," ",
                transLevel,"s with cutoff value ",
                geneFilterCutoff$expression$mean," normalized read counts)",
                sep=""
            )
        }
        if (!is.null(geneFilters$expression$quantile) 
            && !is.na(geneFilters$expression$quantile)) {
            counter <- counter + 1
            msgGeneFiltersExpressionQuantile <- paste(
                latinNumbered[counter]," ",transLevel,"s with read counts ",
                "below the ",100*geneFilters$expression$quantile,
                "<sup>th</sup> quantile of the normalized counts distribution ",
                "(",length(geneFilterResult$expression$quantile)," ",transLevel,
                "s with cutoff value ",geneFilterCutoff$expression$quantile," ",
                "normalized read counts)",sep=""
            )
        }
        if (!is.null(geneFilters$expression$known) 
            && !is.na(geneFilters$expression$known)) {
            counter <- counter + 1
            msgGeneFiltersExpressionKnown <- paste(
                latinNumbered[counter]," ",transLevel,"s with read counts ",
                "below the 90<sup>th</sup> quantile of the counts of the ",
                "following ",transLevel,"s, known to not being expressed from ",
                "the related literature: ",
                paste(geneFilters$expression$known,collapse=", "),
                "(",length(geneFilterResult$expression$known)," ",transLevel,
                "s with cutoff value",geneFilterCutoff$expression$known,
                " normalized read counts)",sep=""
            )
        }
        if (!is.null(geneFilters$expression$custom) 
            && !is.na(geneFilters$expression$custom)) {
            counter <- counter + 1
            msgGeneFiltersExpressionCustom <- paste(
                latinNumbered[counter]," genes not passing the user defined ",
                "filter provided to the metaseqr pipeline (",
                length(geneFilterResult$expression$custom)," ",transLevel,
                "s with cutoff value",geneFilterCutoff$expression$custom,")",
                sep=""
            )
        }
    }
    if (!is.null(geneFilters$biotype)) {
        counter <- counter + 1
        msgGeneFiltersBiotype <- paste(
            latinNumbered[counter]," ",transLevel,"s whose biotype matched ",
            "the following: ",
            paste(
                names(geneFilters$biotype)[which(unlist(geneFilters$biotype))],
                collapse=", "
            ),
            " (",length(geneFilterResult$biotype)," ",transLevel,"s)",sep=""
        )
    }
    if (!is.null(geneFilters$presence)) {
        counter <- counter + 1
        msgGeneFiltersPresence <- paste(
            latinNumbered[counter]," ",transLevel,"s which in ",
            geneFilters$presence$frac*100,"% of samples did not exceed ",
            geneFilters$presence$minCount," counts (",
            length(geneFilterResult$presence)," ",transLevel,"s)",sep=""
        )
        if (geneFilters$presence$perCondition) {
            msgGeneFiltersPresence <- 
                paste(msgGeneFiltersPresence," condition-wise",sep="")
        } else {
            msgGeneFiltersPresence <- 
                paste(msgGeneFiltersPresence," across all samples",sep="")
        }
    }
    
    cat(msgGeneFiltersLength,msgGeneFiltersAvgReads,
        msgGeneFiltersExpressionMedian,msgGeneFiltersExpressionMean,
        msgGeneFiltersExpressionQuantile,msgGeneFiltersExpressionKnown,
        msgGeneFiltersExpressionCustom,msgGeneFiltersBiotype,
        msgGeneFiltersPresence,sep=", ")
    cat(". The total number of ",transLevel,"s excluded due to the ",
        "application of ",transLevel," filters was ",
        sum(as.numeric(sapply(geneFilterResult,length))),". ",sep="")
    cat("The total (unified) number of ",transLevel,"s  excluded due to the ",
        "application of all filters was ",length(theZeros) + length(theDead),
        ". ",sep="")
}

cat("The resulting ",transLevel," counts table was subjected to differential ",
    "expression analysis for the contrasts ",
    paste(gsub("_vs_"," versus ",contrast),collapse=", "),sep="")
if (length(statistics)>1) {
    cat(" using the Bioconductor packages ",paste(unlist(
        reportMessages$stat[statistics],use.names=FALSE),collapse=", "),". ",
        sep="")
    if (metaP!="none") {
        cat("In order to combine the statistical significance from multiple ",
            "algorithms and perform meta-analysis, the ")
            cat(reportMessages$meta[[metaP]],"method was applied. ")
    }
} else {
    cat(" using the Bioconductor package ",reportMessages$stat[[statistics]],
        ". ",sep="")
}

if (!is.na(pcut))
    if (pcut==1) plasm <- 0.05 else plasm <- pcut

if (!is.null(contrast)) {
    cat("The final numbers of differentially expressed ",transLevel,"s were ",
        "(per contrast): ",sep="")
    msgContrast <- character(length(contrast))
    names(msgContrast) <- contrast

    for (cnt in contrast) {
        if (!is.na(pcut) && length(which(sumpList[[cnt]]<plasm))==0) {
            msgContrast[cnt] <- paste(
                "for the contrast ",gsub("_vs_"," versus ",cnt),
                ", no differentially expressed ",transLevel,"s were found",
                " with a p-value threshold of ",plasm,sep=""
            )
        } else if (is.na(pcut)) {
            msgContrast[cnt] <- paste(
                "for the contrast ",gsub("_vs_"," versus ",cnt),
                ", no statistical threshold defined",sep=""
            )
        } else {
            if (adjustMethod != "none") {
                addTextP <- paste(
                    " (",length(which(p.adjust(sumpList[[cnt]],
                    adjustMethod)<plasm)),")",sep=""
                )
                hasFdrText <- " (FDR or adjusted p-value)"
            } else {
                addTextP <- hasFdrText <- NULL
            }
            if (length(strsplit(cnt,"_vs_")[[1]]) == 2) {
                tmp <- log2(makeFoldChange(cnt,sampleList,normGenesExpr[which(
                    sumpList[[cnt]]<plasm),,drop=FALSE],logOffset))
                if (adjustMethod != "none") {
                    areThere <- which(p.adjust(sumpList[[cnt]],
                        adjustMethod)<plasm)
                    if (length(areThere) > 0) {
                        if (length(areThere)==1) {
                            tmpF <- log2(makeFoldChange(cnt,sampleList,
                                normGenesExpr[areThere,,drop=FALSE],logOffset))
                            addTextFu <- 
                                paste(" (",length(which(tmpF>=1)),")",sep="")
                            addTextFd <- 
                                paste(" (",length(which(tmpF<=-1)),")",sep="")
                            addTextFn <- 
                                paste(" (",length(which(abs(tmpF)<1)),")",
                                    sep="")
                        } else {
                            tmpF <- log2(makeFoldChange(cnt,sampleList,
                                normGenesExpr[areThere,,drop=FALSE],logOffset))
                            addTextFu <- 
                                paste(" (",length(which(tmpF>=1)),")",sep="")
                            addTextFd <- 
                                paste(" (",length(which(tmpF<=-1)),")",sep="")
                            addTextFn <- 
                                paste(" (",length(which(abs(tmpF)<1)),")",
                                    sep="")
                        }
                    } else {
                        addTextFu <- addTextFd <- addTextFn <- NULL
                    }
                } else {
                    addTextFu <- addTextFd <- addTextFn <- NULL
                }
                msgContrast[cnt] <- paste(
                    "for the contrast ",gsub("_vs_"," versus ",cnt),", ",
                    length(which(sumpList[[cnt]]<plasm)),addTextP,
                    " statistically significant ",transLevel,"s were found ",
                    "with a p-value",hasFdrText," threshold of ",plasm," and ",
                    "of these, ",length(which(tmp>=1)),addTextFu,
                    " were up-regulated, ",length(which(tmp<=-1)),addTextFd,
                    " were down-regulated and ",addTextFn,
                    " were not differentially expressed according to an ",
                    "absolute fold change cutoff value of 1 in ",
                    "log<sub>2</sub> scale",sep=""
                )
            } else {
                msgContrast[cnt] <- paste(
                    "for the contrast ",gsub("_vs_"," versus ",cnt),", ",
                    length(which(sumpList[[cnt]]<plasm)),addTextP,
                    " differentially expressed ",transLevel,"s were found ",
                    "with a p-value",hasFdrText," threshold of ",plasm," at ",
                    "least in one condition",sep=""
                )
            }
        }
    }
    cat(paste(msgContrast,collapse=", "))
} else {
    cat("No statistical testing was performed")
}

cat(". Literature references for all the algorithms used can be found at the ",
    "end of this report.")
```

## Input options

### Input options

**Read counts file: **
```{r summary_input_options_1}
cat(countsName)
```

**Conditions:** 
```{r summary_input_options_2}
cat(paste(names(sampleList),collapse=", "))
```

**Samples included:** 
```{r summary_input_options_3}
cat(paste(unlist(sampleList),collapse=", "))
```

**Samples excluded:** 
```{r summary_input_options_4}
if (!is.null(excludeList) && !is.na(excludeList)) {
    cat(paste(unlist(excludeList),collapse=", ")) 
} else {
    cat("none")
}

```

**Requested contrasts:** 
```{r summary_input_options_5}
if (!is.null(contrast)) {
    cat(paste(contrast,collapse=", "))
} else {
    cat("No contrasts were defined.")
}
```

**Library sizes:**
```{r summary_input_options_6}
if (!is.null(libsizeList)) {
    cat("<ul>")
    for (n in names(libsizeList)) 
        cat("<li>",paste("<em>",n,"</em>: ",libsizeList[[n]],sep=""),"</li>")
    cat("</ul>")
} else {
    cat("not available")
}
```

**Organism:** 
```{r summary_input_options_7}
cat(reportMessages$org[[org]])
```

**Annotation source:** 
```{r summary_input_options_8}
cat(reportMessages$refdb[[refdb]])
```

**Count type:** 
```{r summary_input_options_9}
cat(countType)
```

```{r summary_input_options_10}
if (countType=="utr" && !is.null(utrOpts)) {
    cat("<strong>3' UTR fraction:</strong> ",utrOpts$frac,"<br/>")
    cat("<strong>3' UTR minimum length:</strong> ",utrOpts$minLength,"bps<br/>")
    cat("<strong>3' UTR downstream:</strong> ",utrOpts$downstream,"bps<br/>")
}
```

**Exon filters:**
```{r summary_input_options_11}
if (!is.null(exonFilters)) {
    cat(paste(names(exonFilters),collapse=", "),"<br/>")
    for (ef in names(exonFilters)) {
        cat("<ul>")
        cat("<li><em><span style=\"font-size:1em\">",ef,"</span></em><ul>",
            sep="")
        for (efp in names(exonFilters[[ef]])) {
            if (length(exonFilters[[ef]][[efp]]) == 1 
                && is.function(exonFilters[[ef]][[efp]])) {
                cat("<li>custom function</li>")
            } else if (length(exonFilters[[ef]][[efp]]) == 1) {
                cat("<li>",paste(efp,exonFilters[[ef]][[efp]],sep=": "),
                    "</li>",sep="")
            } else if (length(exonFilters[[ef]][[efp]]) > 1) {
                cat("<li>",paste(efp,paste(exonFilters[[ef]][[efp]],
                    collapse=", "),sep=": "),"</li>",sep="")
            }
        }
        cat("</ul></li></ul>")
    }
} else {
    cat("none applied")
}
```

```{r summary_input_options_12}
if (!is.null(preset))
    cat("<strong>Analysis preset:</strong>",reportMessages$preset[[preset]])
```

**Gene filters:**
```{r summary_input_options_13}
if (!is.null(geneFilters)) {
    cat(paste(names(geneFilters),collapse=", "),"<br/>")
    for (gf in names(geneFilters)) {
        cat("<ul>")
        cat("<li><em><span style=\"font-size:1em\">",gf,"</span></em><ul>",
            sep="")
        for (gfp in names(geneFilters[[gf]])) {
            if (length(geneFilters[[gf]][[gfp]]) == 1 
                && is.function(geneFilters[[gf]][[gfp]])) {
                cat("<li>custom function</li>")
            } else if (length(geneFilters[[gf]][[gfp]]) == 1) {
                cat("<li>",paste(gfp,geneFilters[[gf]][[gfp]],sep=": "),"</li>",
                    sep="")
            } else if (length(geneFilters[[gf]][[gfp]]) > 1) {
                cat("<li>",paste(gfp,paste(geneFilters[[gf]][[gfp]],
                    collapse=", "),sep=": "),"</li>",sep="")
            }
        }
        cat("</ul></li></ul>")
    }
} else {
    cat("none applied")
}
```

**Filter application:** 
```{r summary_input_options_14}
cat(reportMessages$whenfilter[[whenApplyFilter]])
```

**Normalization algorithm:** 
```{r summary_input_options_15}
cat(reportMessages$norm[[normalization]])
```

**Normalization arguments:**
```{r summary_input_options_16}
if (!is.null(normArgs)) {
    if (normalization == "each") {
        for (n in names(normArgs)) {
            cat("<strong>Statistical arguments for",
                reportMessages$norm[[n]],": </strong>",
                paste(names(normArgs[[n]]),collapse=", "))
            if (length(normArgs[[n]]) > 0) {
                cat("<ul>")
                for (na in names(normArgs[[n]])) {
                    if (length(normArgs[[n]][[na]]) == 1 
                        && is.function(normArgs[[n]][[na]])) {
                        cat("<li>",na,": ",
                            as.character(substitute(normArgs[[na]])),
                        "</li>",sep="")
                    } else if (length(normArgs[[n]][[na]]) == 1) {
                        cat("<li>",paste(na,normArgs[[n]][[na]],sep=": "),
                            "</li>")
                    } else if (length(normArgs[[n]][[na]]) > 1) {
                        cat("<li>",paste(na,paste(normArgs[[n]][[na]],
                            collapse=", "),sep=": "),"</li>")
                    }
                }
                cat("</ul>")
            } else {
                cat("not available or not required")
            }
        }
    } else {
        cat(paste(names(normArgs),collapse=", "),"<br/>")
        cat("<ul>")
        for (na in names(normArgs)) {
            if (length(normArgs[[na]])==1 && is.function(normArgs[[na]])) {
                cat("<li>",as.character(substitute(normArgs[[na]])),"</li>",
                    sep="")
            } else if (length(normArgs[[na]]) == 1) {
                cat("<li>",paste(na,normArgs[[na]],sep=": "),"</li>")
            } else if (length(normArgs[[na]]) > 1) {
                cat("<li>",paste(na,paste(normArgs[[na]],collapse=", "),
                    sep=": "),"</li>")
            }
        }
        cat("</ul>")
    }
} else {
    cat("not available")
}
```

**Statistical algorithm(s):** 
```{r summary_input_options_17}
if (!any(is.na(statistics))) {
    cat(paste(unlist(reportMessages$stat[statistics],use.names=FALSE),
        collapse=", "))
} else {
    cat("No statitical testing performed.")
}
```

```{r summary_input_options_18}
if (!is.null(statArgs)) {
    for (s in names(statArgs)) {
        cat("<strong>Statistical arguments for ",reportMessages$stat[[s]],
            ": </strong>",paste(names(statArgs[[s]]),collapse=", "),sep="")
        if (length(statArgs[[s]]) > 0) {
            cat("<ul>")
            for (sa in names(statArgs[[s]])) {
                if (length(statArgs[[s]][[sa]]) == 1 
                    && is.function(statArgs[[s]][[sa]])) {
                    cat("<li>",sa,": ",as.character(substitute(statArgs[[na]])),
                        "</li>",sep="")
                } else if (length(statArgs[[s]][[sa]])==1) {
                    cat("<li>",paste(sa,statArgs[[s]][[sa]],sep=": "),"</li>")
                } else if (length(statArgs[[s]][[sa]])>1) {
                    cat("<li>",paste(sa,paste(statArgs[[s]][[sa]],
                        collapse=", "),sep=": "),"</li>")
                }
            }
            cat("</ul>")
        } else {
            cat("not available or not required<br/>")
        }
    }
} else {
    cat("<strong>Statistical arguments not available</strong>")
}
```

**Meta-analysis method:** 
```{r summary_input_options_19}
cat(reportMessages$meta[[metaP]])
```

**Multiple testing correction:** 
```{r summary_input_options_20}
cat(reportMessages$adjust[[tolower(adjustMethod)]])
```

**p-value threshold:** 
```{r summary_input_options_21}
if (!is.na(pcut)) cat(pcut) else cat("not available")
```

**Logarithmic tranformation offset: ** 
```{r summary_input_options_22}
cat(logOffset)
```

**Analysis preset:** 
```{r summary_input_options_23}
if (!is.null(preset)) cat(preset) else cat("not available")
```

**Quality control plots:**
```{r summary_input_options_24}
cat(paste(unlist(reportMessages$plots[qcPlots],use.names=FALSE),collapse=", "))
```

**Figure format:** 
```{r summary_input_options_25}
    cat(paste(figFormat,collapse=", "))
```

**Output directory:** 
```{r summary_input_options_26}
if (!is.na(exportWhere)) cat(exportWhere) else cat("default")
```

**Output data: **
```{r summary_input_options_27}
cat(paste(unlist(reportMessages$export[exportWhat],use.names=FALSE),
    collapse=", "))
```

**Output scale(s):** 
```{r summary_input_options_28}
cat(paste(unlist(reportMessages$export[exportScale],use.names=FALSE),
    collapse=", "))
```

**Output values:** 
```{r summary_input_options_29}
cat(paste(unlist(reportMessages$export[exportValues],use.names=FALSE),
    collapse=", "))
```

**Output statistics:**
```{r summary_input_options_30}
cat(paste(unlist(reportMessages$export[exportStats],use.names=FALSE),
    collapse=", "))
```

**Total run time:**
```{r summary_input_options_31}
cat(execTime)
```

## Filtering

<h3>Filtered 
```{r summary_filtered_what_1}
cat(transLevel,"s",sep="")
``` 
</h3>

**Number of filtered**
```{r summary_filtered_what_2}
cat("<strong>",transLevel,"s: </strong>",sep="")
```
```{r summary_filtered_howmany_1}
cat(length(theZeros) + length(theDead))
```
*which is the **union** of*

* Filtered because of zero reads:
```{r summary_filtered_howmany_2}
cat(length(theZeros))
```
* Filtered because of exon filters:
```{r summary_filtered_howmany_3}
cat(sum(as.numeric(sapply(exonFilterResult,length))))
if (sum(as.numeric(sapply(exonFilterResult,length))) != 0) {
    cat("<em> which is the <strong>union</strong> of</em>")
    cat("<ul>")
    for (n in names(exonFilterResult)) {
        cat("<li>",n,": ",length(exonFilterResult[[n]]),"</li>")
    }
    cat("</ul>")
}
```
* Filtered because of gene filters:
```{r summary_filtered_howmany_4}
cat(length(unique(unlist(geneFilterResult))))
```
*which is the **union** of*
```{r summary_filtered_howmany_5}
cat("<ul>")
for (n in names(geneFilterResult)) {
    if (!is.list(geneFilterResult[[n]])) {
        if (!is.null(geneFilterResult[[n]]))
            cat("<li>",n,": ",length(unlist(geneFilterResult[[n]])),
                " genes with filter cutoff value ",geneFilterCutoff[[n]],
                "</li>",sep="")
    } else {
        cat("<li>",n,": ",length(unlist(geneFilterResult[[n]])),
            " ",transLevel,"s further decomposed to (filter name, filtered ",
            transLevel,"s, filter cutoff):</li>",sep="")
        cat("<ul>")
        for (nn in names(geneFilterResult[[n]])) {
            if (!is.null(geneFilterResult[[n]][[nn]]))
                cat("<li>",nn,": ",length(unlist(geneFilterResult[[n]][[nn]])),
                " ",transLevel,"s with filter cutoff value ",
                paste(geneFilterCutoff[[n]][[nn]],collapse=", "),"</li>",sep="")
        }
        cat("</ul>")
    }
}
cat("</ul>")
```

## Differential expression

<h3>Differentially expressed 
```{r summary_de_what_1}
cat(transLevel,"s",sep="")
``` 
</h3>

**Number of differentially expressed **
```{r summary_de_what_2}
cat("<strong>",transLevel,"s per contrast: </strong>",sep="")
if (!is.null(contrast) && !any(is.na(statistics))) {
    if (!is.na(pcut) && pcut==1)
        cat("The p-value cutoff during the analysis was set to 1 so as to ",
            "retrieve the total ",transLevel," list which passed the filtering ",
            "procedure. Each ",transLevel," in the list is accompanied by its ",
            "statistical scores (p-value, FDR, etc.)")

    cat("<ul>")
    for (cnt in contrast) {
        cat("<li><strong>",cnt,": </strong>",sep="")
        if (!is.na(pcut) && length(which(sumpList[[cnt]]<pcut)) == 0) {
            cat("no differentially expressed ",transLevel,"s","</li>",sep="")
        } else if (is.na(pcut)) {
            cat("no statistical threshold defined","</li>")
        } else {
            if (!is.na(pcut) && pcut==1) {
                plasm <- 0.05
            } else {
                plasm <- pcut
            }
            if (adjustMethod!="none") {
                addTextP <- paste("(",length(which(p.adjust(sumpList[[cnt]],
                    adjustMethod) < plasm)),")",sep="")
                hasFdrText <- "(FDR or adjusted p-value)"
            } else {
                addTextP <- hasFdrText <- NULL
            }
            if (length(strsplit(cnt,"_vs_")[[1]]) == 2) {
                tmpP <- log2(makeFoldChange(cnt,sampleList,normGenesExpr[which(
                    sumpList[[cnt]] < plasm),,drop=FALSE],logOffset))
                if (adjustMethod != "none") {
                    areThere <- which(p.adjust(sumpList[[cnt]],adjustMethod)<plasm)
                    if (length(areThere)>0) {
                        if (length(areThere) == 1) {
                            tmpF <- log2(makeFoldChange(cnt,sampleList,
                                normGenesExpr[areThere,,drop=FALSE],logOffset))
                            addTextFu <- 
                                paste(" (",length(which(tmpF>=1)),")",sep="")
                            addTextFd <- 
                                paste(" (",length(which(tmpF<=-1)),")",sep="")
                            addTextFn <- 
                                paste(" (",length(which(abs(tmpF)<1)),")",sep="")
                        } else {
                            tmpF <- log2(makeFoldChange(cnt,sampleList,
                                normGenesExpr[areThere,,drop=FALSE],logOffset))
                            addTextFu <- 
                                paste(" (",length(which(tmpF>=1)),")",sep="")
                            addTextFd <- 
                                paste(" (",length(which(tmpF<=-1)),")",sep="")
                            addTextFn <- 
                                paste(" (",length(which(abs(tmpF)<1)),")",sep="")
                        }
                    } else {
                        addTextFu <- addTextFd <- addTextFn <- NULL
                    }
                } else {
                    addTextFu <- addTextFd <- addTextFn <- NULL
                }
                cat(length(which(sumpList[[cnt]]<plasm))," ",addTextP,
                    " statistically significant ",transLevel,"s of which ",
                    length(which(tmpP>=1)),addTextFu," up regulated, ",
                    length(which(tmpP<=-1)),addTextFd," down regulated and ",
                    length(which(abs(tmpP)<1)),addTextFn," not differentially ",
                    "expressed according to a p-value ",hasFdrText," threshold of ",
                    plasm," and an absolute fold change cutoff value of 1 in ",
                    "log<sub>2</sub> scale.",sep="")
            } else {
                cat(length(which(sumpList[[cnt]]<plasm))," ",addTextP,
                    " statistically significant, differentially expressed in at",
                    " least one condition at a p-value ",hasFdrText,
                    " threshold of ",plasm,".",sep="")
            }
            if (length(statistics)>1 && metaP != "none") {
                cat(" These numbers refer to the combined analysis performed by ",
                    "metaseqR2. Per statistical algorithm, the differentially ",
                    "expressed ",transLevel,"s are:",sep="")
                cat("<ul>")
                for (s in statistics) {
                    cat("<li><em>",reportMessages$stat[[s]],": </em>",sep="")
                    if (adjustMethod!="none") {
                        addTextP <- paste("(",length(which(p.adjust(
                            cpList[[cnt]][,s],adjustMethod)<plasm)),")",sep="")
                        hasFdrText <- "(FDR or adjusted p-value)"
                    } else {
                        addTextP <- hasFdrText <- NULL
                    }
                    if (length(strsplit(cnt,"_vs_")[[1]]) == 2) {
                        tmpP <- log2(makeFoldChange(cnt,sampleList,
                            normGenesExpr[which(cpList[[cnt]][,s]<plasm),,
                            drop=FALSE],logOffset))
                        if (adjustMethod != "none") {
                            tmpF <- log2(makeFoldChange(cnt,sampleList,
                                normGenesExpr[which(p.adjust(cpList[[cnt]][,s],
                                adjustMethod)<plasm),,drop=FALSE],logOffset))
                            addTextFu <- 
                                paste("(",length(which(tmpF>=1)),")",sep="")
                            addTextFd <- 
                                paste("(",length(which(tmpF<=-1)),")",sep="")
                            addTextFn <- 
                                paste("(",length(which(abs(tmpF)<1)),")",sep="")
                        } else {
                            addTextFu <- addTextFd <- addTextFn <- NULL
                        }
                        cat(length(which(cpList[[cnt]][,s]<plasm))," ",addTextP,
                            " statistically significant ",transLevel,"s of which ",
                            length(which(tmpP>=1))," ",addTextFu," up regulated, ",
                            length(which(tmpP<=-1)),addTextFd," down regulated ",
                            "and ",length(which(abs(tmpP)<1))," ",addTextFn,
                            " not differentially expressed according to a p-value ",
                            hasFdrText," threshold of ",plasm," and an absolute ",
                            "fold change cutoff value of 1 in log<sub>2</sub> ",
                            "scale.",sep="")
                    } else {
                        cat(length(which(cpList[[cnt]][,s]<plasm))," ",addTextP,
                            " statistically significant, differentially expressed ",
                            "in at least one condition at a p-value ",hasFdrText,
                            " threshold of ",plasm,".",sep="")
                    }
                    cat("</li>")
                }
                cat("</ul>")
            }
        }
    }
    cat("</ul>")
} else {
    cat("No statistical testing was performed. If contrasts were defined,",
        "then fold changes are available in the Results section.")
}
```

## Command

The differential expression analysis and this report were generated using the
following command:

```{r fun_call}
cat("<pre><code>")
cat(paste(FUN_CALL,collapse="<br/>"))
cat("</code></pre>")
```

```{r display_targets}
if (!is.null(theList)) {
    # First write targets
    tarDf <- metaseqR2:::.writeTargets(theList,outfile=file.path(
        PROJECT_PATH$main,"tracks","targets.txt"))
    tarDf$filename <- basename(as.character(tarDf$filename))
    
    # Download link
    cat("<br/>")
    cat("You can download the targets file from <strong>",
      "<a href='tracks/targets.txt' download target='_blank'>here</a>",
      "</strong><br/>",sep="")

    # Then render a table with targets
    cat("<br/>")
    cat("The following table summarizes the targets file used for the",
        "analysis. Do not forget to prepend the path to your BAM files in the",
        "<srong>filename</strong> column (also in the file that can be",
        "downloaded above).<br/>")
    print(kable(tarDf))
}
```

```{r summary_run_log}
if (runLog) {
    cat("<br/>The above command generated the following log output:<br/><br/>")
    cat("<div id=\"summary_log_container\">")
    logString <- paste(readLines(file.path(PROJECT_PATH$logs,
        "metaseqr_run.log")),collapse="---EOL---")
    logString <- gsub("\\$","->",logString)
    cat(gsub("---EOL---","<br/>",logString))
    cat("</div><br/>")
} else {
    cat("<h4>No logging requested</h4>")
}
```

## `r if (createTracks) 'Tracks'`

```{r tracks_1}
if (createTracks) {
    o <- metaseqR2:::getUcscOrganism(org)
    cat("You can use this ")
    if (trackInfo$stranded) {
        cat("<a href='https://genome.ucsc.edu/cgi-bin/hgTracks?genome=",o,
            "&hubUrl=",tLink,"' target='_blank'><strong>link</strong>",
            "</a>",sep="")
    } else {
        cat("<a href='https://genome.ucsc.edu/cgi-bin/hgTracks?db=",o,
            "&hgct_customText=",tLink,"' target='_blank'><strong>link</strong>",
            "</a>",sep="")
    }
    cat(" to load a UCSC Genome Browser session with the tracks derived from ",
        "this analysis. If stranded mode was chosen, a trackhub will be ",
        "loaded, otherwise, simple tracks will be loaded.",sep="")
    cat("<br/>")
}
```

```{r tracks_2}
if (createTracks) {
    if (trackInfo$stranded) {
        o <- metaseqR2:::getUcscOrganism(org)
        plusLinks <- paste0(trackInfo$urlBase,"/",o,"/",samples,"_plus.bigWig")
        minusLinks <- paste0(trackInfo$urlBase,"/",o,"/",samples,
            "_minus.bigWig")
        cat("You can download individual bigWig files, one for each sample,",
            "using the following list:<br/><br/>")
        cat("<strong>Plus (+) strand</strong><br/>")
        for (i in 1:length(plusLinks))
            cat("<a href='",plusLinks[i],"'>",samples[i],"</a><br/>",sep="")
        cat("<br/><strong>Minus (-) strand</strong><br/>")
        for (i in 1:length(minusLinks))
            cat("<a href='",minusLinks[i],"'>",samples[i],"</a><br/>",sep="")
        cat("<br/>")
    } else {
        tLines <- readLines(file.path(trackExportPath,"tracks.txt"))
        tmp <- strsplit(tLines," ")
        tNames <- sapply(tmp,function(x) {
            return(strsplit(x[6],"=")[[1]][2])
        })
        tLinks <- paste0(trackInfo$urlBase,"/",tNames,".bigWig")
        cat("You can download individual bigWig files, one for each sample,",
            "using the following list:<br/>")
        for (i in 1:length(tNames))
            cat("<a href='",tLinks[i],"'>",tNames[i],"</a><br/>",sep="")
        cat("<br/>")
        cat("You can manually load bigWig files in UCSC Genome Browser using ",
            "the following track lines:<br/><br/>")
        cat("<pre><code>")
        cat(paste(tLines,collapse="<br/>"))
        cat("</code></pre><br/>")
    }
}
```

```{r decide_qc}
if (any(qcPlots %in% qualPlots)) {
    pandoc.header("Quality control",level=1)
    pandoc.header("Quality control figures {.tabset .tabset-pills}",level=2)
    cat("The following figures summarize the quality control steps and ",
        "assessment performed by the ```metaseqr2``` pipeline. Each figure ",
        "category is accompanied by an explanatory text. All figures are ",
        "interactive wih additional controls on the top right of the figure.",
        sep="")
}
                
```

```{r analysis_figures_mds}
if ("mds" %in% qcPlots) {
    pandoc.header("MDS",level=3)
    
    ## Get chart data as serialized JSON
    #json <- diagplotMds(geneCounts,sampleList,output="json")
    
    cat("<h4><strong>Multidimensional scaling</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$mds,
        "<br/><br/></div>",sep="")
    
    # Container div
    cat("<div class=\"hc-rect-sm\" id=\"mds_container\"></div>",sep="")
    ## Render the chart
    #cat("<script>$('#mds_container').highcharts(",json,");</script>",sep="")
    # A hidden element to trigger upon page load and fetch the plot
    cat("<button id=\"_mds_trigger\" class=\"hidden-element\"></button>",sep="")
}
```

```{r analysis_figures_biodetection}
if ("biodetection" %in% qcPlots) {
    pandoc.header("Biodetection",level=3)
    
    cat("<h4><strong>Biotype detection</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$biodetection,
        "<br/><br/></div>",sep="")
        
    # Create a selector for each sample
    items <- paste("<option value=",paste("biodetection",samples,sep="_"),">",
        samples,"</option>",sep="")
    cat("<div>")
    cat("<div class=\"hc-selector-label\">Select a sample to display plot for",
        "</div>",sep="")
    cat("<select id=\"biodetection_selector\">",items,"</select>",sep="")
    cat("</div>")
    
    # Container div
    cat("<div class=\"hc-rect-sm\" id=\"biodetection_container\"></div>",sep="")
    
    #if (reportDb == "dexie") {
    #   # Get chart data as list
    #   log <- capture.output({
    #       json <- diagplotNoiseq(geneCounts,sampleList,covars=covarsRaw,
    #           whichPlot="biodetection",output="json")
    #   })
    #   
    #   # Add extra data, serialize and import the plots to Dexie
    #   preImport <- vector("list",length(json))
    #   for (i in 1:length(json)) {
    #       preImport[[i]] <- list(
    #           name=paste("biodetection",samples[i],sep="_"),
    #           type="biodetection",
    #           subtype="generic",
    #           json=json[[samples[i]]]
    #       )
    #   }
    #   toImport <- toJSON(preImport,auto_unbox=TRUE,null="null")
    #   
    #   # Import script
    #   cat("<script type=\"text/javascript\">")
    #   cat("db.plots.bulkAdd(",toImport,");",sep="")
    #   cat("</script>")
    #   
    #   # Initialize first plot with serialized JSON
    #   cat("<script>$('#biodetection_container').highcharts(",
    #       toJSON(preImport[[1]]$json,auto_unbox=TRUE,null="null"),
    #       ");</script>",sep="")
    #}
}
```

```{r analysis_figures_countsbio}
if ("countsbio" %in% qcPlots) {
    pandoc.header("Biocounts",level=3)
    
    cat("<h4><strong>Biotype representation</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$countsbio,
        "<br/><br/></div>",sep="")
    
    cat("<h4><em>Biotypes within samples</em></h4>")
    
    # Create a selector for each sample
    items <- paste("<option value=",paste("countsbio",samples,sep="_"),">",
        samples,"</option>",sep="")
    cat("<div>")
    cat("<div class=\"hc-selector-label\">Select a sample to display plot for",
        "</div>",sep="")
    cat("<select id=\"countsbio_sample_selector\">",items,"</select>",sep="")
    cat("</div>")
    
    # Container div - sample
    cat("<div class=\"hc-rect-sm\" id=\"countsbio_sample_container\"></div>",
        sep="")
    
    cat("<br/><h4><em>Biotype representation across samples</em></h4>")
    
    # Create a selector for each biotype
    items <- paste("<option value=",paste("countsbio",biotypes,sep="_"),">",
        biotypes,"</option>",sep="")
    cat("<div>")
    cat("<div class=\"hc-selector-label\">Select a biotype to display plot for",
        "</div>",sep="")
    cat("<select id=\"countsbio_biotype_selector\">",items,"</select>",sep="")
    cat("</div>")
    
    # Container div - biotype
    cat("<div class=\"hc-rect-sm\" id=\"countsbio_biotype_container\">",
        "</div>",sep="")    
}
```

```{r analysis_figures_saturation}
if ("saturation" %in% qcPlots) {
    pandoc.header("Saturation",level=3)
    
    cat("<h4><strong>Biotype representation</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$saturation,
        "<br/><br/></div>",sep="")
    
    cat("<h4><em>Read saturation per biotype for all samples</em></h4>")
    
    # Create a selector for each sample
    items <- paste("<option value=",paste("saturation",samples,sep="_"),">",
        samples,"</option>",sep="")
    cat("<div>")
    cat("<div class=\"hc-selector-label\">Select a sample to display plot for",
        "</div>",sep="")
    cat("<select id=\"saturation_sample_selector\">",items,"</select>",sep="")
    cat("</div>")
    
    # Container div - sample
    cat("<div class=\"hc-rect-sm\" id=\"saturation_sample_container\"></div>",
        sep="")
    
    cat("<br/><h4><em>Read saturation per sample for all biotypes</em></h4>")
    
    # Create a selector for each biotype
    items <- paste("<option value=",paste("saturation",biotypes,sep="_"),">",
        biotypes,"</option>",sep="")
    cat("<div>")
    cat("<div class=\"hc-selector-label\">Select a biotype to display plot for",
        "</div>",sep="")
    cat("<select id=\"saturation_biotype_selector\">",items,"</select>",sep="")
    cat("</div>")
    
    # Container div - biotype
    cat("<div class=\"hc-rect-sm\" id=\"saturation_biotype_container\">",
        "</div>",sep="")
}
```

```{r analysis_figures_readsnoise}
if ("readnoise" %in% qcPlots) {
    pandoc.header("Reads noise",level=3)
    
    cat("<h4><strong>RNA-Seq reads noise</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$readnoise,
        "<br/><br/></div>",sep="")
    cat("<div class=\"hc-rect-sm\" id=\"readsnoise_container\"></div>",
        sep="")
    cat("<button id=\"_readnoise_trigger\" class=\"hidden-element\"></button>",
        sep="")
}
```

```{r analysis_figures_correl}
if ("correl" %in% qcPlots) {
    pandoc.header("Correlation",level=3)
    
    cat("<h4><strong>Pairwise sample correlations</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$correl,
        "<br/><br/></div>",sep="")
    
    corMat <- cor(geneCounts)
    if (!is.null(colnames(geneCounts)))
        colnames(corMat) <- colnames(geneCounts)
    
    n <- dim(corMat)[1]
    labs <- matrix(NA,n,n)
    for (i in 1:n)
        for (j in 1:n)
            labs[i,j] <- sprintf("%.2f",corMat[i,j])
    if (n <= 5) {
        notecex <- 1.2
    } else if (n > 5 & n < 10) {
        notecex <- 0.9
    } else {
        notecex <- 0.7
    }
    
    h <- list(heatmaply(corMat,revC=TRUE,trace="none",symm=TRUE,Colv=TRUE,
        cellnote=labs,colors=colorRampPalette(c("yellow","grey","blue")),
        k_col=length(sampleList),k_row=length(sampleList)) %>%
        layout(width=640,height=480))
    setNames(h,NULL)
    htmltools::tagList(tags$div(id="cormap",class="coheatmap-container",h[[1]]))
    #heatmaply(corMat,revC=TRUE,trace="none",symm=TRUE,Colv=TRUE,
    #   cellnote=labs,colors=colorRampPalette(c("yellow","grey","blue")),
    #   k_col=length(sampleList),k_row=length(sampleList)) %>%
    #   layout(width=640,height=480)
}
```

```{r analysis_figures_pairwise}
if ("pairwise" %in% qcPlots) {
    pandoc.header("Pairwise",level=3)
    
    cat("<h4><strong>Pairwise sample correlations</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$pairwise,
        "<br/><br/></div>",sep="")
        
    nams <- colnames(geneCounts)
    n <- ncol(geneCounts)
    plotNames <- character(n*(n-1)/2)
    counter <- 0
    for (i in 1:(n-1)) {
        for (j in (i+1):n) {
            counter <- counter + 1
            plotNames[counter] <- paste(nams[i],nams[j],sep="_vs_")
        }
    }
    
    # Create a selector for each sample
    items <- paste("<option value=",plotNames,">",gsub("_vs_"," vs ",plotNames),
        "</option>",sep="")
    cat("<div>")
    cat("<div class=\"hc-selector-label\">Select a pair to display plot for",
        "</div>",sep="")
    cat("<select id=\"pairwise_selector\">",items,"</select>",sep="")
    cat("</div>")
    
    cat("<h4><em>X-Y plots</em></h4>")
    # Container div - sample
    cat("<div class=\"hc-rect-sm\" id=\"pairwise_xy_container\"></div>",
        sep="")
    
    cat("<br/><h4><em>M-D plots</em></h4>")
    # Container div - sample
    cat("<div class=\"hc-rect-sm\" id=\"pairwise_md_container\"></div>",
        sep="")
}
```

```{r analysis_figures_filtered}
if ("filtered" %in% qcPlots) {
    pandoc.header("Filtered",level=3)
    
    cat("<h4><strong>Chromosome and biotype distribution of filtered ",
        transLevel,"s</strong></h4>",sep="")
    cat("<div class=\"figure-hint\">",reportMessages$explain$filtered,
        "<br/><br/></div>",sep="")
    cat("<h4>Chromosome distribution of filtered",transLevel,"s</h4>",sep="")
    cat("<div class=\"hc-rect-sm\" id=\"chromosome_distr_container\"></div>",
        sep="")
    cat("<h4>Biotype distribution of filtered ",transLevel,"s</h4>",sep="")
    cat("<div class=\"hc-rect-sm\" id=\"biotype_distr_container\"></div>",
        sep="")
    cat("<button id=\"_filtered_trigger\" class=\"hidden-element\"></button>",
        sep="")
}
```

```{r decide_norm}
if (any(qcPlots %in% normPlots)) {
    pandoc.header("Normalization",level=1)
    pandoc.header("Normalization assessment figures {.tabset .tabset-pills}",
        level=2)
    cat("The following figures allow for the assessment of the ",
        "normalization procedures performed by the ```metaseqr2``` pipeline. ",
        "Each figure category is accompanied by an explanatory text. All ",
        "figures are interactive wih additional controls on the top right ",
        "corner of the figure.",sep="")
}
```

```{r analysis_figures_boxplot}
if ("boxplot" %in% qcPlots) {
    pandoc.header("Boxplots",level=3)
    
    cat("<h4><strong>Boxplots</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$boxplot,
        "<br/><br/></div>",sep="")
    cat("<div class=\"hc-rect-sm\" id=\"boxplot_unorm_container\"></div>",
        sep="")
    cat("<div class=\"hc-rect-sm\" id=\"boxplot_norm_container\"></div>",
        sep="")
    cat("<br/><br/>")
    cat("<button id=\"_boxplot_trigger\" class=\"hidden-element\"></button>",
        sep="")
}
```

```{r analysis_figures_gcbias}
if ("gcbias" %in% qcPlots) {
    pandoc.header("GC bias",level=3)
    
    cat("<h4><strong>GC bias assessment plots</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$gcbias,
        "<br/><br/></div>",sep="")
    cat("<div class=\"hc-rect-sm\" id=\"gcbias_unorm_container\"></div>",
        sep="")
    cat("<div class=\"hc-rect-sm\" id=\"gcbias_norm_container\"></div>",
        sep="")
    cat("<br/><br/>")
    cat("<button id=\"_gcbias_trigger\" class=\"hidden-element\"></button>",
        sep="")
}
```

```{r analysis_figures_lengthbias}
if ("lengthbias" %in% qcPlots) {
    pandoc.header("Length bias",level=3)
    
    cat("<h4><strong>Length bias assessment plots</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$lengthbias,
        "<br/><br/></div>",sep="")
    cat("<div class=\"hc-rect-sm\" id=\"lengthbias_unorm_container\"></div>",
        sep="")
    cat("<div class=\"hc-rect-sm\" id=\"lengthbias_norm_container\"></div>",
        sep="")
    cat("<br/><br/>")
    cat("<button id=\"_lengthbias_trigger\" class=\"hidden-element\"></button>",
        sep="")
}
```

```{r analysis_figures_meandiff}
if ("meandiff" %in% qcPlots) {
    pandoc.header("Mean-Difference",level=3)
    
    cat("<h4><strong>Mean-difference plots for normalization assessment",
        "</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$meandiff,
        "<br/><br/></div>",sep="")
    
    # Create a selector for each sample
    namList <- vector("list",length(sampleList))
    names(namList) <- names(sampleList)
    for (n in names(sampleList)) {
        pairMatrix <- combn(1:length(sampleList[[n]]),2)
        nm <- character(0)
        for (i in 1:ncol(pairMatrix)) {
            s1 <- sampleList[[n]][pairMatrix[1,i]]
            s2 <- sampleList[[n]][pairMatrix[2,i]]
            nm <- c(nm,paste(s1,"_vs_",s2,sep=""))
        }
        namList[[n]] <- nm
    }
    
    html <- ""
    for (n in names(namList)) {
        nams <- namList[[n]]
        items <- paste("<option value=",nams,">",gsub("_vs_"," vs ",nams),
            "</option>",sep="")
        html <- paste(html,"<optgroup label=\"",n,"\">",items,"</optgroup>",
            sep="")
    }
    
    cat("<div>")
    cat("<div class=\"hc-selector-label\">Select a pair to display plots for",
        "</div>",sep="")
    cat("<select id=\"meandiff_selector\">",html,"</select>",sep="")
    cat("</div>")
    
    # Container div - un-normalized
    cat("<div class=\"hc-rect-sm\" id=\"meandiff_unorm_container\"></div>",
        sep="")
    # Container div - normalized
    cat("<div class=\"hc-rect-sm\" id=\"meandiff_norm_container\"></div>",
        sep="") 
}
```

```{r analysis_figures_meanvar}
if ("meanvar" %in% qcPlots) {
    pandoc.header("Mean-Variance",level=3)
    
    cat("<h4><strong>Mean-variance plot for normalization assessment",
        "</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$meanvar,
        "<br/><br/></div>",sep="")
    cat("<div class=\"hc-rect-sm\" id=\"meanvar_unorm_container\"></div>",
        sep="")
    cat("<div class=\"hc-rect-sm\" id=\"meanvar_norm_container\"></div>",
        sep="")
    cat("<button id=\"_meanvar_trigger\" class=\"hidden-element\"></button>",
        sep="")
}
```

```{r analysis_figures_rnacomp}
if ("rnacomp" %in% qcPlots) {
    pandoc.header("Rna composition",level=3)
    
    cat("<h4><strong>RNA composition plot</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$rnacomp,
        "<br/><br/></div>",sep="")
        
    cat("<div class=\"hc-rect-sm\" id=\"rnacomp_unorm_container\"></div>",
        sep="")
    cat("<div class=\"hc-rect-sm\" id=\"rnacomp_norm_container\"></div>",sep="")
    cat("<button id=\"_rnacomp_trigger\" class=\"hidden-element\"></button>",
        sep="")
}
```

```{r decide_stat}
if (any(qcPlots %in% statPlots)) {
    pandoc.header("Statistics",level=1)
    pandoc.header(
        "Differential expression assessment figures {.tabset .tabset-pills}",
        level=2)
    cat("The following figures allow for the assessment of the statistical ",
        "testing procedures performed by the ```metaseqr2``` pipeline. Each ",
        "figure category is accompanied by an explanatory text. All figures ",
        "are interactive wih additional controls on the top right corner of ",
        "the figure.",sep="")
}
```

```{r analysis_figures_volcano}
if ("volcano" %in% qcPlots) {
    pandoc.header("Volcano",level=3)
    
    cat("<h4><strong>Volcano plots</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$volcano,
        "<br/><br/></div>",sep="")
        
    nn <- names(contrastList)
    namc <- character(0)
    for (n in nn) {
        fc <- log2(makeFoldChange(n,sampleList,normGenesExpr[1:3,],1))
        for (contr in colnames(fc))
            namc <- c(namc,contr)
    }
    
    # Create a selector for each contrast
    items <- paste("<option value=",paste("volcano",namc,sep="_"),">",
        gsub("_vs_"," vs ",namc),"</option>",sep="")
    cat("<div>")
    cat("<div class=\"hc-selector-label\">Select a contrast to display plot ",
        "for </div>",sep="")
    cat("<select id=\"volcano_selector\">",items,"</select>",sep="")
    cat("</div>")
    
    # Container div
    cat("<div class=\"hc-rect-sm\" id=\"volcano_container\"></div>",sep="")
}
```

```{r analysis_figures_mastat}
if ("mastat" %in% qcPlots) {
    pandoc.header("MA",level=3)
    
    cat("<h4><strong>Mean-Difference (MA) plots</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$mastat,
        "<br/><br/></div>",sep="")
    
    nn <- names(contrastList)
    namc <- character(0)
    for (n in nn) {
        m <- log2(makeFoldChange(n,sampleList,normGenesExpr[1:3,],1))
        for (contr in colnames(m))
            namc <- c(namc,contr)
    }
    
    # Create a selector for each contrast
    items <- paste("<option value=",paste("mastat",namc,sep="_"),">",
        gsub("_vs_"," vs ",namc),"</option>",sep="")
    cat("<div>")
    cat("<div class=\"hc-selector-label\">Select a contrast to display plot ",
        "for </div>",sep="")
    cat("<select id=\"mastat_selector\">",items,"</select>",sep="")
    cat("</div>")
    
    # Container div
    cat("<div class=\"hc-rect-sm\" id=\"mastat_container\"></div>",sep="")
}
```

```{r analysis_figures_deheatmap}
if ("deheatmap" %in% qcPlots) {
    pandoc.header("Heatmap",level=3)
    
    cat("<h4><strong>Differential expression heatmaps</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$deheatmap,
        "<br/><br/></div>",sep="")
    
    # Create a selector for each contrast
    items <- paste("<option value=",
        paste("deheatmap",gsub("\\.","__",names(contrastList)),sep="_"),">",
        gsub("_vs_"," vs ",names(contrastList)),"</option>",sep="")
    scal <- paste("<option value=",c("asis","zscore"),">",
        c("log2 reads","z-scores"),"</option>",sep="")
    cat("<div class=\"row\"><div class=\"col-sm-12\">",sep="")
    cat("<div class=\"row\"><div class=\"col-sm-6\">")
    cat("<div class=\"hc-selector-label\">Select a contrast to display ",
        "heatmap for </div>",sep="")
    cat("<select id=\"heatmap_contrast_selector\">",items,"</select>",sep="")
    cat("</div><div class=\"col-sm-6\">")
    cat("<div class=\"pull-right\">")
    cat("<div class=\"hc-selector-label\">Select a scale to display heatmap ",
        "for </div>",sep="")
    cat("<select id=\"heatmap_scale_selector\">",scal,"</select>",sep="")
    cat("</div></div></div></div></div>")
    
    hList <- vector("list",length(contrastList)*2)
    hNames <- character(length(contrastList)*2)
    hInd <- 0
    for (cnt in names(contrastList)) {
        s <- names(unlist(contrastList[[cnt]]))
        if (is.na(pcut))
            pcut <- 0.05
        ind <- which(sumpList[[cnt]]<=pcut)
        if (length(ind) > 0) {
            mat <- as.matrix(normGenesExpr[ind,s])
        } else {
            mat <- as.matrix(normGenesExpr[,s])
        }
        if (!is.null(reportTop)) {
            topg <- ceiling(reportTop*nrow(mat))
            mat <- mat[1:topg,,drop=FALSE]
        }
        # As is scale
        hInd <- hInd + 1
        hNames[hInd] <- paste0("deheatmap_",gsub("\\.","__",cnt),"_asis")
        hList[[hInd]] <- heatmaply(nat2log(mat),trace="none",colors=bluered(64),
            labRow=rep(NA,nrow(mat)),
            main=paste("DEG heatmap",cnt,"log2 reads")) %>% 
            layout(width=720,height=840)
        # z-scores
        M <- t(scale(t(nat2log(mat))))
        if (any(is.infinite(M)))
            M[which(is.infinite(M))] <- min(M)
        if (any(is.na(M)))
            M[which(is.na(M))] <- 0
        hInd <- hInd + 1
        hNames[hInd] <- paste0("deheatmap_",gsub("\\.","__",cnt),"_zscore")
        hList[[hInd]] <- heatmaply(M,trace="none",colors=bluered(64),
            labRow=rep(NA,nrow(mat)),
            main=paste("DEG heatmap",cnt,"z-scores")) %>% 
            layout(width=720,height=840)
    }
    setNames(hList,NULL)
    
    # Dirty hack
    toEval <- "htmltools::tagList("
    hItems <- character(length(hList))
    for (i in 1:length(hList))
        hItems[i] <- paste0("tags$div(id='",hNames[i],
            "', class='deheatmap-container',hList[[",i,"]])")
    toEval <- paste0(toEval,paste(hItems,collapse=","),")")
    eval(parse(text=toEval))
    
    #htmltools::tagList(
    #   tags$div(id=paste("deheatmap",gsub("\\.","__",names(contrastList)[1]),
    #       sep="_"),class="deheatmap-container",hList[[1]]),
    #   tags$div(id=paste("deheatmap",gsub("\\.","__",names(contrastList)[2]),
    #       sep="_"),class="deheatmap-container",hList[[2]])
    #)
}
```

```{js deheatmap_render}
// Chunk to initialize the display of the DE heatmaps
var hids = $('.deheatmap-container').map(function(index) {
    return(this.id);
});
$('.deheatmap-container').hide(0);
$('#'+hids[0]).show();
```

```{r analysis_figures_biodist}
if ("biodist" %in% qcPlots) {
    pandoc.header("Biodist",level=3)
    
    cat("<h4><strong>Chromosome and biotype distributions of differentially ",
        "expressed ",transLevel,"s</strong></h4>",sep="")
    cat("<div class=\"figure-hint\">",reportMessages$explain$biodist,
        "<br/><br/></div>",sep="")
    
    nn <- names(contrastList)
    
    # Create a selector for each contrast
    items <- paste("<option value=",paste("biodist",nn,sep="_"),">",
        gsub("_vs_"," vs ",nn),"</option>",sep="")
    cat("<div>")
    cat("<div class=\"hc-selector-label\">Select a contrast to display plot ",
        "for </div>",sep="")
    cat("<select id=\"biodist_selector\">",items,"</select>",sep="")
    cat("</div>")
    
    # Containers   
    cat("<h4>Chromosome distribution of differentially expressed ",transLevel,
        "s</h4>",sep="")
    cat("<div class=\"hc-rect-sm\" id=\"biodist_chromosome_container\"></div>",
        sep="")
    cat("<h4>Biotype distribution of differentially expressed ",transLevel,
        "s</h4>",sep="")
    cat("<div class=\"hc-rect-sm\" id=\"biodist_biotype_container\"></div>",
        sep="")
}
```

```{r analysis_figures_deregulogram}
if ("deregulogram" %in% qcPlots) {
    pandoc.header("Deregulogram",level=3)
    
    cat("<h4><strong>Deregulograms</strong></h4>")
    cat("<div class=\"figure-hint\">",reportMessages$explain$deregulogram,
        "<br/><br/></div>",sep="")
    
    cntPairs <- combn(names(contrastList),2)
    namc <- character(ncol(cntPairs))
    counter <- 0
    for (i in 1:ncol(cntPairs)) {
        counter <- counter + 1
        namc[counter] <- paste0(cntPairs[1,i],"__",cntPairs[2,i])
    }
    
    # Create a selector for each pair of contrasts
    items <- paste("<option value=",paste("deregulogram",namc,sep="_"),">",
        gsub("__"," against ",namc),"</option>",sep="")
    cat("<div>")
    cat("<div class=\"hc-selector-label\">Select a pair of contrasts to ",
        "display plot for </div>",sep="")
    cat("<select id=\"deregulo_selector\">",items,"</select>",sep="")
    cat("</div>")
    
    # Container div
    cat("<div class=\"hc-rect-sm\" id=\"deregulo_container\"></div>",sep="")
}
```

```{r analysis_figures_statvenn}
if ("statvenn" %in% qcPlots) {
    pandoc.header("StatVenn",level=3)
    
    cat("<h4><strong>Venn diagram of differentially expressed ",transLevel,
        "s</strong></h4>",sep="")
    cat("<div class=\"figure-hint\">",reportMessages$explain$statvenn,
        "<br/><br/></div>",sep="")
        
    # Create a selector for each contrast
    nn <- names(contrastList)
    items <- paste("<option value=",paste("statvenn",nn,sep="_"),">",
        gsub("_vs_"," vs ",nn),"</option>",sep="")
    deupdo <- paste("<option value=",c("dereg","up","down"),">",
        c("Deregulated","Up regulated","Down downregulated"),"</option>",sep="")
    cat("<div class=\"row\"><div class=\"col-sm-8\">")
    cat("<div class=\"row\"><div class=\"col-sm-6\">")
    cat("<div class=\"hc-selector-label\">Select a contrast to display Venn ",
        "diagram for </div>",sep="")
    cat("<select id=\"statvenn_contrast_selector\">",items,"</select>",sep="")
    cat("</div><div class=\"col-sm-6\">")
    cat("<div class=\"hc-selector-label\">Select a direction to display Venn ",
        "diagram for </div>",sep="")
    cat("<select id=\"statvenn_direction_selector\">",deupdo,"</select>",sep="")
    cat("</div></div></div></div>")
    
    cat("<div class=\"row\">")
    cat("<div class=\"col-sm-8\"><div id=\"jvenn_stat_container\"></div></div>")
    cat("<div class=\"col-sm-4\">")
    cat("<div class=\"hc-selector-label\">Click on a number on Venn diagrams ",
        "to display the respective genes</div>",sep="")
    cat("<textarea id=\"jvenn_stat_list\" class=\"jvenn_list\" readonly>",
        "</textarea></div>",sep="")
    cat("</div>")
}

```{r analysis_figures_foldvenn}
if ("foldvenn" %in% qcPlots) {
    pandoc.header("FoldVenn",level=3)
    
    cat("<h4><strong>Venn diagram of differentially expressed ",transLevel,
        "s</strong></h4>",sep="")
    cat("<div class=\"figure-hint\">",reportMessages$explain$foldvenn,
        "<br/><br/></div>",sep="")
        
    # Create a selector for each contrast
    if (length(statistics) > 1) {
        nn <- c(statistics,metaP)
    } else {
        nn <- statistics
    }
    
    items <- paste("<option value=",paste("foldvenn",nn,sep="_"),">",nn,
        "</option>",sep="")
    deupdo <- paste("<option value=",c("dereg","up","down"),">",
        c("Deregulated","Up regulated","Down downregulated"),"</option>",sep="")
    cat("<div class=\"row\"><div class=\"col-sm-8\">")
    cat("<div class=\"row\"><div class=\"col-sm-6\">")
    cat("<div class=\"hc-selector-label\">Select an algorithm to display Venn ",
        "diagram for </div>",sep="")
    cat("<select id=\"foldvenn_algo_selector\">",items,"</select>",sep="")
    cat("</div><div class=\"col-sm-6\">")
    cat("<div class=\"hc-selector-label\">Select a direction to display Venn ",
        "diagram for </div>",sep="")
    cat("<select id=\"foldvenn_direction_selector\">",deupdo,"</select>",sep="")
    cat("</div></div></div></div>")
    
    cat("<div class=\"row\">")
    cat("<div class=\"col-sm-8\"><div id=\"jvenn_fold_container\"></div></div>")
    cat("<div class=\"col-sm-4\">")
    cat("<div class=\"hc-selector-label\">Click on a number on Venn diagrams ",
        "to display the respective genes</div>",sep="")
    cat("<textarea id=\"jvenn_fold_list\" class=\"jvenn_list\" readonly>",
        "</textarea></div>",sep="")
    cat("</div>")
}
```

# Results

## Tables of differentially expressed genes

The following tables allow for a quick exploration of the results of the
statistical analysis performed by the ```metaseqr2``` pipeline. If no
statistical testing or contrasts requested, just ignore any respective texts
and jump to tables or download the results.

```{r results_tables_header}
if (is.null(reportTop)) {
    topText <- paste("all the statistically significant ",  transLevel,"s. ",
        sep="")
} else {
    topText <- paste("the top ",round(100*reportTop),"% statistically ",
        "significant ",transLevel,"s. ",sep="")
}
restText <- paste("Use the download links below each table to retrieve the ",
    "total list of differentially expressed ",transLevel,"s or the whole ",
    transLevel," list of the selected genome irrespective of differential ",
    "expression.",sep="")
cat("Each table presents ",topText,restText,sep="")
cat("Furthermore each table can be searched using the search field on the ",
    "top right and you can also find the following information:<br/><br/>",
    sep="")

cat("<ul>")
cat("<li>The <em>chromosome</em> column is linked to the genomic location of ",
    "the ",transLevel," and opens a new tab/window to the UCSC Genome Browser",
    "</li>",sep="")
cat("<li>The *gene_id* column opens a link to the respective full annotation ",
    "source (only for Ensembl and RefSeq)</li>",sep="")
cat("<li>The background of the *p_value* and *FDR* columns displays a bar ",
    "with length proportional to the significance of each ",transLevel,"</li>",
    sep="")
cat("<li>The background color of the *fold change (_vs_)* column(s) displays ",
    "shows the deregulation of each ",transLevel," and is proportional to ",
    "the deregulation strength (red for up- green for down-regulation)</li>",
    sep="")
cat("<li>The background of the rest columns (condition average expression) ",
    "displays a bar with length proportional to the expression strength ",
    "of each condition</li>",sep="")
cat("</ul>")
```

```{r dt_hack, include=FALSE}
DT::datatable(NULL,extensions="Buttons")
```

```{r results_tables}
# Create a selector for each contrast
if (!is.null(contrast)) {
    items <- paste("<option value=",paste("detable",gsub("\\.","__",
        names(contrastList)),sep="_"),">",gsub("_vs_"," vs ",
        names(contrastList)),"</option>",sep="")
} else {
    items <- "<option value=detable_no_contrast>No contrast</option>"
}
cat("<hr><div>")
cat("<div class=\"hc-selector-label\">Select a contrast to display ",
    "DEG table for </div>",sep="")
cat("<select id=\"detable_selector\">",items,"</select>",sep="")
cat("</div><br/>")

if (!is.null(contrast) && !any(is.na(statistics))) {
    for (cnt in contrast) {
        cntn <- gsub("_vs_"," vs ",cnt)
        #pandoc.header(cntn,level=3)
        
        cat("<div class='detable-helper-wrapper' id='detable_",
            gsub("\\.","__",cnt),"'>",sep="")
        
        cat("<h4>DEG table for the contrast <strong>",cntn,"</strong></h4>",
            sep="")
        cat("<div class=\"figure-hint\">The following table presents ",topText,
            " for the contrast <strong>",cntn,"</strong>.</div>",sep="")
        
        # 15 colors should be enough for most situations...
        sColors <- c("#97F9F9","#A4DEF9","#C1E0F7","#CFBAE1","#C59FC9",
            "#EDEEC9","#DDE7C7","#BFD8BD","#98C9A3","#77BFA3","#7BDFF2",
            "#B2F7EF","#EFF7F6","#F7D6E0","#F2B5D4")
            
        # We do not need GC content really...
        gci <- grep("gc_content",colnames(reportTables[[cnt]]))
        if (length(gci) > 0)
            reportTables[[cnt]] <- reportTables[[cnt]][,-gci]
            
        # Fold change and condition means should always exist in this table 
        # defined by us...
        pi <- grep("p-value",colnames(reportTables[[cnt]]))
        di <- grep("FDR",colnames(reportTables[[cnt]]))
        fi <- grep("_vs_",colnames(reportTables[[cnt]]))
        # If more than one statistics applied, the 1st two from fi are meta 
        # p-value and meta FDR
        if (length(statistics) > 1)
            fi <- fi[-c(1,2)]
        #ci <- numeric(length(sampleList))
        #N <- names(sampleList)
        ci <- numeric(length(contrastList[[cnt]]))
        N <- unique(unlist(contrastList[[cnt]],use.names=FALSE))
        
        if (length(grep("rpgm_normalized_mean_counts_",
            colnames(reportTables[[cnt]]))) > 0) {
            for (i in 1:length(N))
                ci[i] <- grep(paste0("rpgm_normalized_mean_counts_",N[i]),
                    colnames(reportTables[[cnt]]))
        } else {
            for (i in 1:length(N))
                ci[i] <- grep(N[i],colnames(reportTables[[cnt]]),fixed=TRUE)
        }
        
        # Get values before rounding for styling
        brks <- quantile(reportTables[[cnt]][,fi],probs=seq(.05,.95,.05),
            na.rm=TRUE)
        preClrs <- col2rgb(rev(redgreen(length(brks)+1)))
        clrs <- paste("rgb(",apply(preClrs,2,paste0,collapse=","),")",sep="")
        pRangeForColor <- 1-as.numeric(reportTables[[cnt]][,pi])
        fRangeForColor <- 1-as.numeric(reportTables[[cnt]][,di])
        styleBars <- character(length(ci))
        for (i in 1:length(ci))
            styleBars[i] <- styleColorBar(range(reportTables[[cnt]][,ci[i]]),
                sColors[i])
        
        # After setting colors, format the table for the report
        if (!is.numeric(version)) # Not defined in call
            version <- NULL
        reportTables[[cnt]] <- .formatForReport(reportTables[[cnt]],o=org,
            r=refdb,v=version)
        # Hack to prevent the header name taking 2 rows because of 
        # hyphenation(?)
        names(reportTables[[cnt]])[pi] <- "p_value"
        
        # Dirty hack
        # https://stackoverflow.com/questions/32018521/
        # shiny-use-stylecolorbar-with-data-from-two-data-frames
        reportTables[[cnt]] <- 
            cbind(pRangeForColor,fRangeForColor,reportTables[[cnt]])
        jspf <- paste0("function(row,data) {",
            "var value = data[0];",
            "var backgroundValueP =",styleColorBar(pRangeForColor,
                "lightblue")[1],";",
            "var value = data[1];",
            "var backgroundValueF =",styleColorBar(fRangeForColor,
                "lightsalmon")[1],";",
            "$('td',row).eq(",pi-1,").css({",
                "'background': backgroundValueP,",
                "'background-repeat': 'no-repeat',",
                "'background-position': 'center',",
                "'background-size': '98% 88%'",
            "});",
            "$('td',row).eq(",di-1,").css({",
                "'background': backgroundValueF,",
                "'background-repeat': 'no-repeat',",
                "'background-position': 'center',",
                "'background-size': '98% 88%'",
            "});",
            "for (var i=",fi[1],";i<=",fi[length(fi)],";i++) {",
                "var value = data[i+1];",
                "$('td',row).eq(i-1).css({",
                    "'color': '#FFFFFF',",
                    "'font-weight': 'bold',",
                    "'background-color': ",styleInterval(brks,clrs),
                "});",
            "}")
        
        for (i in 1:length(ci)) {
            jsa <- paste0("var value = data[",ci[i]+1,"];",
                "$('td',row).eq(",ci[i]-1,").css({",
                    "'background': ",styleBars[i],",",
                    "'background-repeat': 'no-repeat',",
                    "'background-position': 'center',",
                    "'background-size': '98% 88%'",
                "});")
            jspf <- paste0(jspf,jsa)
        }
        jspf <- paste0(jspf,"}")
        
        theTable <- DT::datatable(
            reportTables[[cnt]],
            rownames=FALSE,
            width="95%",
            height=460,
            style="bootstrap",
            class="table-condensed table-striped table-responsive",
            escape=FALSE,
            elementId=paste("detable_",gsub("\\.","__",cnt),"_",sep=""),
            extensions="Buttons",
            options=list(
                scrollX=TRUE,
                order=list(list(pi+1,"asc")),
                columnDefs=list(
                    list(
                        targets=0:1,
                        visible=FALSE
                    )
                ),
                dom='Bfrtip',
                #buttons=I('colvis'),
                buttons=list(
                    list(
                        extend='colvis',
                        #exclude=0:1,
                        text="Show/Hide columns"
                    )
                ),
                rowCallback=JS(jspf)
            )
        ) #%>% formatStyle(
            #names(reportTables[[cnt]])[fi+2],
            #backgroundColor=styleInterval(brks,clrs),
            #fontWeight="bold",
            #color="#FFFFFF"
        #) #%>% formatStyle(
            #names(reportTables[[cnt]])[pi+2],
            #background=styleColorBar(pRangeForColor,"lightblue"),
            #fontWeight="bold",
            #backgroundSize="98% 88%",
            #backgroundRepeat="no-repeat",
            #backgroundPosition="center"
        #)
        print(htmltools::tagList(tags$div(class="detable-container",theTable)))
        
        cat("<br/><br/><div class=\"figure-hint link-box\">")
        cat("<strong><a href=\"lists/metaseqr_sig_out_",cnt,".txt.gz\"",
            " download>Download</a> the DEG result list for ",cnt,
            ".</strong><br/>",sep="")
        if (!is.null(geneCountsZero) || !is.null(geneCountsDead))
            cat("<strong><a href=\"lists/metaseqr_all_out_",cnt,".txt.gz\"",
            " download>Download</a> the whole result list for ",cnt,
            ".</strong><br/>",sep="")
        cat("</div><br/><br/>")
        
        cat("</div>")
    }
} else if (!is.null(contrast) && any(is.na(statistics))) {
    for (cnt in contrast) {
        cntn <- gsub("_vs_"," vs ",cnt)
        
        cat("<div class='detable-helper-wrapper' id='detable_",
            gsub("\\.","__",cnt),"'>",sep="")
        
        cat("<h4>DEG table for the contrast <strong>",cntn,"</strong></h4>",
            sep="")
        cat("<div class=\"figure-hint\">The following table presents ",topText,
            " for the contrast <strong>",cntn,"</strong>.</div>",sep="")
        
        # 15 colors should be enough for most situations...
        sColors <- c("#97F9F9","#A4DEF9","#C1E0F7","#CFBAE1","#C59FC9",
            "#EDEEC9","#DDE7C7","#BFD8BD","#98C9A3","#77BFA3","#7BDFF2",
            "#B2F7EF","#EFF7F6","#F7D6E0","#F2B5D4")
            
        # We do not need GC content really...
        gci <- grep("gc_content",colnames(reportTables[[cnt]]))
        if (length(gci) > 0)
            reportTables[[cnt]] <- reportTables[[cnt]][,-gci]
            
        # Fold change and condition means should always exist in this table 
        # defined by us...
        fi <- grep("_vs_",colnames(reportTables[[cnt]]))
        #ci <- numeric(length(contrastList[[cnt]]))
        #N <- unique(unlist(contrastList[[cnt]],use.names=FALSE))
        ci <- numeric(length(names(sampleList)))
        N <- names(sampleList)
        
        if (length(grep("rpgm_normalized_mean_counts_",
            colnames(reportTables[[cnt]]))) > 0) {
            for (i in 1:length(N))
                ci[i] <- grep(paste0("rpgm_normalized_mean_counts_",N[i]),
                    colnames(reportTables[[cnt]]))
        } else {
            for (i in 1:length(N))
                ci[i] <- grep(N[i],colnames(reportTables[[cnt]]),fixed=TRUE)
        }
        
        # Get values before rounding for styling
        brks <- quantile(reportTables[[cnt]][,fi],probs=seq(.05,.95,.05),
            na.rm=TRUE)
        preClrs <- col2rgb(rev(redgreen(length(brks)+1)))
        clrs <- paste("rgb(",apply(preClrs,2,paste0,collapse=","),")",sep="")
        styleBars <- character(length(ci))
        for (i in 1:length(ci))
            styleBars[i] <- styleColorBar(range(reportTables[[cnt]][,ci[i]]),
                sColors[i])
        
        # After setting colors, format the table for the report
        if (!is.numeric(version)) # Not defined in call
            version <- NULL
        reportTables[[cnt]] <- .formatForReport(reportTables[[cnt]],o=org,
            r=refdb,v=version)
        
        # Dirty hack
        # https://stackoverflow.com/questions/32018521/
        # shiny-use-stylecolorbar-with-data-from-two-data-frames
        reportTables[[cnt]] <- cbind(reportTables[[cnt]])
        jspf <- paste0("function(row,data) {",
            "for (var i=",fi[1],";i<=",fi[length(fi)],";i++) {",
                "var value = data[i-1];",
                "$('td',row).eq(i-1).css({",
                    "'color': '#FFFFFF',",
                    "'font-weight': 'bold',",
                    "'background-color': ",styleInterval(brks,clrs),
                "});",
            "}")
        
        for (i in 1:length(ci)) {
            jsa <- paste0("var value = data[",ci[i]-1,"];",
                "$('td',row).eq(",ci[i]-1,").css({",
                    "'background': ",styleBars[i],",",
                    "'background-repeat': 'no-repeat',",
                    "'background-position': 'center',",
                    "'background-size': '98% 88%'",
                "});")
            jspf <- paste0(jspf,jsa)
        }
        jspf <- paste0(jspf,"}")
        
        theTable <- DT::datatable(
            reportTables[[cnt]],
            rownames=FALSE,
            width="95%",
            height=460,
            style="bootstrap",
            class="table-condensed table-striped table-responsive",
            escape=FALSE,
            elementId=paste("detable_",gsub("\\.","__",cnt),"_",sep=""),
            extensions="Buttons",
            options=list(
                scrollX=TRUE,
                dom='Bfrtip',
                buttons=list(
                    list(
                        extend='colvis',
                        text="Show/Hide columns"
                    )
                ),
                rowCallback=JS(jspf)
            )
        )
        print(htmltools::tagList(tags$div(class="detable-container",theTable)))
        
        cat("<br/><br/><div class=\"figure-hint link-box\">")
        cat("<strong><a href=\"lists/metaseqr_sig_out_",cnt,".txt.gz\"",
            " download>Download</a> the DEG result list for ",cnt,
            ".</strong><br/>",sep="")
        if (!is.null(geneCountsZero) || !is.null(geneCountsDead))
            cat("<strong><a href=\"lists/metaseqr_all_out_",cnt,".txt.gz\"",
            " download>Download</a> the whole result list for ",cnt,
            ".</strong><br/>",sep="")
        cat("</div><br/><br/>")
        
        cat("</div>")
    }
} else {
    cat("<div class='detable-helper-wrapper' id='detable_no_contrast'>")
    cat("<h4>Quantification table for the run</h4>")
    
    # 15 colors should be enough for most situations...
    sColors <- c("#97F9F9","#A4DEF9","#C1E0F7","#CFBAE1","#C59FC9",
        "#EDEEC9","#DDE7C7","#BFD8BD","#98C9A3","#77BFA3","#7BDFF2",
        "#B2F7EF","#EFF7F6","#F7D6E0","#F2B5D4")
        
    # We do not need GC content really...
    gci <- grep("gc_content",colnames(reportTables[[1]]))
    if (length(gci) > 0)
        reportTables[[1]] <- reportTables[[1]][,-gci]
        
    #ci <- numeric(length(unlist(sampleList)))
    #N <- unique(unlist(sampleList,use.names=FALSE))
    ci <- numeric(length(names(sampleList)))
    N <- names(sampleList)
    
    if (length(grep("rpgm_normalized_mean_counts_",
        colnames(reportTables[[1]]))) > 0) {
        for (i in 1:length(N))
            ci[i] <- grep(paste0("rpgm_normalized_mean_counts_",N[i]),
                colnames(reportTables[[1]]))
    } else {
        for (i in 1:length(N))
            ci[i] <- grep(N[i],colnames(reportTables[[1]]),fixed=TRUE)
    }
    
    # Get values before rounding for styling
    styleBars <- character(length(ci))
    for (i in 1:length(ci))
        styleBars[i] <- styleColorBar(range(reportTables[[1]][,ci[i]]),
            sColors[i])
    
    # After setting colors, format the table for the report
    if (!is.numeric(version)) # Not defined in call
        version <- NULL
    reportTables[[1]] <- .formatForReport(reportTables[[1]],o=org,r=refdb,
        v=version)
    
    # Dirty hack
    # https://stackoverflow.com/questions/32018521/
    # shiny-use-stylecolorbar-with-data-from-two-data-frames
    jspf <- paste0("function(row,data) {")
    for (i in 1:length(ci)) {
        jsa <- paste0("var value = data[",ci[i]-1,"];",
            "$('td',row).eq(",ci[i]-1,").css({",
                "'background': ",styleBars[i],",",
                "'background-repeat': 'no-repeat',",
                "'background-position': 'center',",
                "'background-size': '98% 88%'",
            "});")
        jspf <- paste0(jspf,jsa)
    }
    jspf <- paste0(jspf,"}")
    
    theTable <- DT::datatable(
        reportTables[[1]],
        rownames=FALSE,
        width="95%",
        height=460,
        style="bootstrap",
        class="table-condensed table-striped table-responsive",
        escape=FALSE,
        elementId="detable_no_contrast",
        extensions="Buttons",
        options=list(
            scrollX=TRUE,
            dom='Bfrtip',
            buttons=list(
                list(
                    extend='colvis',
                    text="Show/Hide columns"
                )
            ),
            rowCallback=JS(jspf)
        )
    )
    print(htmltools::tagList(tags$div(class="detable-container",theTable)))
    
    cat("<br/><br/><div class=\"figure-hint link-box\">")
    cat("<strong><a href=\"lists/metaseqr_sig_out.txt.gz\"",
        " download>Download</a> the result list.</strong><br/>",sep="")
    if (!is.null(geneCountsZero) || !is.null(geneCountsDead))
        cat("<strong><a href=\"lists/metaseqr_all_out.txt.gz\"",
        " download>Download</a> the whole result list.</strong><br/>",sep="")
    cat("</div><br/><br/>")
    
    cat("</div>")
}
```

```{js detable_render}
var tids = $('.detable-helper-wrapper').map(function(index) {
    return(this.id);
});
$('.detable-helper-wrapper').hide(0);
$('#'+tids[0]).show();

// The only way to align columns...
var timer = setInterval(function() {
    if ($('#'+tids[0]+"_").find("table").length > 0) {
        if ($('#'+tids[0]+"_").find("table")[1].id !== undefined) {
            var id = $('#'+tids[0]+"_").find("table")[1].id;
            $('#'+id).DataTable().columns.adjust();
            clearInterval(timer);
        }
    }
},100);
```

```{r export_links}
if (exportCountsTable) {
    cat("<br/><div class=\"figure-hint link-box\">")
    if (file.exists(file.path(PROJECT_PATH[["lists"]],
        "raw_counts_table.txt.gz")))
        cat("<strong><a href=\"lists/raw_counts_table.txt.gz\" download>",
            "Download</a> the raw read counts table for the experiment.",
            "</strong><br/>",sep="")
    cat("<strong><a href=\"lists/normalized_counts_table.txt.gz\"",
        " download>Download</a> the normalized read counts table for the ",
        "experiment.</strong><br/>",sep="")
    cat("</div>")
}

if (length(qcPlots) > 0) {
    ltxt <- paste(paste("<a href=\"plots/metaseqr_figures_",figFormat,
        ".zip\" download>",figFormat,"</a>",sep=""),collapse=", ")
    cat("<div class=\"figure-hint link-box\">")
    cat("<strong>Get all the figures in ",ltxt," format.</strong>")
    cat("</div>")
}
```

# References

```{r references}
refs <- unique(c(
    reportMessages$references$main,
    reportMessages$references$filein[[fileType]],
    reportMessages$references$norm[[normalization]],
    unlist(reportMessages$references$stat[statistics],use.names=FALSE),
    unlist(reportMessages$references$figure[qcPlots],use.names=FALSE),
    reportMessages$references$multiple[[adjustMethod]],
    reportMessages$references$meta[[metaP]]
))
cat("<div class=\"figure-hint\" style=\"margin-left:25px;\"><ol>")
for (r in refs)
    cat("<li>",r,"</li>",sep="")
cat("</ol></div>")
```

```{js bind_event_changes}
$("#biodetection_selector").on('change',function() {
    var name = this.value;
    getPlotFromDb({name: name,type: "biodetection",subtype: "generic"},
        "biodetection_container");
});

$("#countsbio_sample_selector").on('change',function() {
    var name = this.value;
    getPlotWithFunFromDb({name: name,type: "countsbio",subtype: "sample"},
        "countsbio_sample_container");
});

$("#countsbio_biotype_selector").on('change',function() {
    var name = this.value;
    getPlotWithFunFromDb({name: name,type: "countsbio",subtype: "biotype"},
        "countsbio_biotype_container");
});

$("#saturation_sample_selector").on('change',function() {
    var name = this.value;
    getPlotWithFunFromDb({name: name,type: "saturation",subtype: "sample"},
        "saturation_sample_container");
});

$("#saturation_biotype_selector").on('change',function() {
    var name = this.value;
    getPlotWithFunFromDb({name: name,type: "saturation",subtype: "biotype"},
        "saturation_biotype_container");
});

$("#pairwise_selector").on('change',function() {
    var name = this.value;
    getPlotFromDb({name: name,type: "pairwise",subtype: "xy"},
        "pairwise_xy_container");
    getPlotFromDb({name: name,type: "pairwise",subtype: "md"},
        "pairwise_md_container");
});

$("#meandiff_selector").on('change',function() {
    var name = this.value;
    getPlotFromDb({name: name,type: "meandiff",subtype: "unorm"},
        "meandiff_unorm_container");
    getPlotFromDb({name: name,type: "meandiff",subtype: "norm"},
        "meandiff_norm_container");
});

$("#volcano_selector").on('change',function() {
    var name = this.value;
    getPlotFromDb({name: name,type: "volcano",subtype: "generic"},
        "volcano_container");
});

$("#mastat_selector").on('change',function() {
    var name = this.value;
    getPlotFromDb({name: name,type: "mastat",subtype: "generic"},
        "mastat_container");
});

$("#deregulo_selector").on('change',function() {
    var name = this.value;
    getPlotFromDb({name: name,type: "deregulogram",subtype: "generic"},
        "deregulo_container");
});

$("#heatmap_contrast_selector").on('change',function() {
    var name = this.value;
    var suffix = $("#heatmap_scale_selector").val();
    var newName = name + "_" + suffix;
    $(".deheatmap-container").hide(0);
    $("#"+newName).show();
});

$("#heatmap_scale_selector").on('change',function() {
    var name = this.value;
    var prefix = $("#heatmap_contrast_selector").val();
    var newName = prefix + "_" + name;
    $(".deheatmap-container").hide(0);
    $("#"+newName).show();
});

$("#biodist_selector").on('change',function() {
    var name = this.value;
    getPlotFromDb({name: name,type: "biodist",subtype: "chromosome"},
        "biodist_chromosome_container");
    getPlotFromDb({name: name,type: "biodist",subtype: "biotype"},
        "biodist_biotype_container");
});

$("#statvenn_contrast_selector").on('change',function() {
    var name = this.value;
    var suffix = $("#statvenn_direction_selector").val();
    var newName = name + "_" + suffix;
    var newSubtype = "stat_" + suffix;
    getVennFromDb({name: newName,type: "statvenn",subtype: newSubtype},
        "jvenn_stat_container");
});

$("#statvenn_direction_selector").on('change',function() {
    var name = this.value;
    var prefix = $("#statvenn_contrast_selector").val();
    var newName = prefix + "_" + name;
    var newSubtype = "stat_" + name;
    getVennFromDb({name: newName,type: "statvenn",subtype: newSubtype},
        "jvenn_stat_container");
});

$("#foldvenn_algo_selector").on('change',function() {
    var name = this.value;
    var suffix = $("#foldvenn_direction_selector").val();
    var newName = name + "_" + suffix;
    var newSubtype = "fold_" + suffix;
    getVennFromDb({name: newName,type: "foldvenn",subtype: newSubtype},
        "jvenn_fold_container");
});

$("#foldvenn_direction_selector").on('change',function() {
    var name = this.value;
    var prefix = $("#foldvenn_algo_selector").val();
    var newName = prefix + "_" + name;
    var newSubtype = "fold_" + name;
    getVennFromDb({name: newName,type: "foldvenn",subtype: newSubtype},
        "jvenn_fold_container");
});

$("#detable_selector").on('change',function() {
    var name = this.value;
    $(".detable-helper-wrapper").hide(0);
    $("#"+name).show();
    
    // Hack to make the table visible (htmlwidget known issue)
    window.dispatchEvent(new Event('resize'));
    // Hack to align the column headers
    var id = $('#'+name+"_").find("table")[1].id;
    $('#'+id).DataTable().columns.adjust();
});

// Hidden buttons
$("#_mds_trigger").on('click',function() {
    getPlotFromDb({name: "MDS",type: "mds",subtype: "generic"},"mds_container");
});
$("#_readnoise_trigger").on('click',function() {
    getPlotFromDb({name: "ReadNoise",type: "readnoise",subtype: "generic"},
        "readsnoise_container");
});
$("#_filtered_trigger").on('click',function() {
    getPlotFromDb({name: "filtered_chromosome",type: "filtered",
        subtype: "chromosome"},"chromosome_distr_container");
    getPlotFromDb({name: "filtered_biotype",type: "filtered",
        subtype: "biotype"},"biotype_distr_container");
});
$("#_boxplot_trigger").on('click',function() {
    getPlotFromDb({name: "Boxplot",type: "boxplot",subtype: "unorm"},
        "boxplot_unorm_container");
    getPlotFromDb({name: "Boxplot",type: "boxplot",subtype: "norm"},
        "boxplot_norm_container");
});
$("#_gcbias_trigger").on('click',function() {
    getPlotFromDb({name: "GCBias",type: "gcbias",subtype: "unorm"},
        "gcbias_unorm_container");
    getPlotFromDb({name: "GCBias",type: "gcbias",subtype: "norm"},
        "gcbias_norm_container");
});
$("#_lengthbias_trigger").on('click',function() {
    getPlotFromDb({name: "LengthBias",type: "lengthbias",subtype: "unorm"},
        "lengthbias_unorm_container");
    getPlotFromDb({name: "LengthBias",type: "lengthbias",subtype: "norm"},
        "lengthbias_norm_container");
});
$("#_meanvar_trigger").on('click',function() {
    getPlotFromDb({name: "MeanVar",type: "meanvar",subtype: "unorm"},
        "meanvar_unorm_container");
    getPlotFromDb({name: "MeanVar",type: "meanvar",subtype: "norm"},
        "meanvar_norm_container");
});
$("#_rnacomp_trigger").on('click',function() {
    getPlotFromDb({name: "RnaComp",type: "rnacomp",subtype: "unorm"},
        "rnacomp_unorm_container");
    getPlotFromDb({name: "RnaComp",type: "rnacomp",subtype: "norm"},
        "rnacomp_norm_container");
});

// Initialize some by changing plots (harmless for dexie, required for sqlite)
$('#_mds_trigger').click();
$("#_readnoise_trigger").click();
$("#_filtered_trigger").click();
$("#_boxplot_trigger").click();
$("#_gcbias_trigger").click();
$("#_lengthbias_trigger").click();
$("#_meanvar_trigger").click();
$("#_rnacomp_trigger").click();

$('#biodetection_selector').trigger('change');
$('#countsbio_sample_selector').trigger('change');
$('#countsbio_biotype_selector').trigger('change');
$('#saturation_sample_selector').trigger('change');
$('#saturation_biotype_selector').trigger('change');
$('#pairwise_selector').trigger('change');
$('#meandiff_selector').trigger('change');
$("#volcano_selector").trigger('change');
$("#mastat_selector").trigger('change');
$("#deregulo_selector").trigger('change');
$("#biodist_selector").trigger('change');
if ($("#statvenn_contrast_selector").length > 0) {
    $("#statvenn_contrast_selector").trigger('change');
}
if ($("#foldvenn_algo_selector").length > 0) {
    $("#foldvenn_algo_selector").trigger('change');
}
```
